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Citations for Thermo Fisher :
701 - 750 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the control and ADARB1 encoding pcDNA3 constructs using Lipofectamine 3000 (Invitrogen) and analyzed 72hrs post-transfection.
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured at 37°C and 5% CO2 in DMEM (Thermo Fisher Scientific; 11965092) supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral supernatants were generated by transient transfection of HEK293 cells according to Lipofectamine ™ 2000 (Invitrogen) transfection protocols and harvested 48 h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... BAC DNA was transfected into HEK293/ACE2/TMPRSS2 cells using lipofectamine 3000 (Thermo Fisher, cat. L3000001). Two days after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293 cells were transfected at 70 to 80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, #L3000015) and incubated at 37°C for 2 days ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC;CRL-1573) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 11965-092) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... HEK293 cells were transfected with PX459 and pCR-Blunt II TOPO plasmids by Lipofectamine 3000 (Invitrogen) for 72 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... both plasmids containing sgRNAs were transfected into naïve HEK293 cells by Lipofectamine 3000 (Thermo Fisher Scientific) as instructed by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed in HEK293 GnTI- cells cultured in the FreeStyle 293 media (Thermo Fisher Scientific) supplemented with 1% (v/v ...
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bioRxiv - Immunology 2024Quote: HEK293 cells were cultured in DMEM medium (Cytiva, Austria) containing 8% fetal bovine serum (Gibco, USA), 25,000 U penicillin and 25 mg streptomycin (Paneko ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting constructs were transfected into HEK293 cells using the Lipofectamine 3000 Reagent (Invitrogen, Cat#L3000001) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HEK293 (ATCC CRL-1573) and Gi-KO HEK239A cells (22) were cultured in DMEM (Gibco 1196511) supplemented with 10% FBS (UCSF Cell Culture Facility) ...
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bioRxiv - Biochemistry 2024Quote: HEK293S GnTl− cells were used for protein expression and were cultured in Freestyle 293 medium (GIBCO) supplemented with 2% fetal bovine serum ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed in HEK293 GnTI- cells cultured in the FreeStyle 293 media (Thermo Fisher Scientific) supplemented with 1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 and HEK293T cells were purchased from ATCC and cultured in DMEM media (Gibco, 11965-092) containing L-glutamine and 4.5 g/L D-glucose ...
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bioRxiv - Neuroscience 2020Quote: ... Neuron like differentiation was induced by switching the medium to Neurobasal (Gibco) supplemented with 2% L-Glutamine (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... spheroids containing ring-like structures were extracted using a Scalpel (ThermoFisher, #1000044), ensuring that excess Matrigel is removed ...
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bioRxiv - Genomics 2020Quote: Flp-In T-REx 293 (HEK293-derived; Invitrogen) and HEK293T (American Type Culture Collection ...
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bioRxiv - Biophysics 2021Quote: ... was expressed in HEK293-EBNA (Thermo Fisher Scientific) cells and purified as described previously[26–28].
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bioRxiv - Systems Biology 2022Quote: The Flp-in T-REx HEK293 system (Invitrogen) was utilized for both stable plasmid transfection as well as doxycycline-induced expression ...
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bioRxiv - Biochemistry 2020Quote: ... cells were simultaneously incubated with secondary Alexa Fluor 488 goat anti-mouse immunoglobulin G (IgG) (Invitrogen, A-11001, 1:1000) and Streptavidin-Alexa Fluor 647 IgG (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with Alexa-594 coupled goat anti-rabbit immunoglobulin G (IgG) secondary antibodies (1:5000; Molecular Probes) in 1x PBS for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: African green monkey kidney epithelial cells (Vero, ATCC) and HEK293 T cells (ATCC) were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco Invitrogen) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 15 × 104 wildtype HEK293 cells or SMAP1 KO A2 cells were transfected with GFP-vWF with Lipofectamine LTX (Thermo Fisher) by reverse transfection ...
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bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
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bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
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bioRxiv - Genetics 2024Quote: HEK293 (XX female) cells and HepG2 cells were cultured in high glucose Dulbecco’s modified eagle medium (DMEM) (41965, Thermo Fisher) and supplemented with 10% fetal bovine serum (10270 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... donkey anti-rabbit immunoglobulin G–AlexaFluor647 (Thermo Fisher Scientific; Cat. No. A32795), 10 μg/ml Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... we used non-crossreacting A488-labeled donkey anti-mouse immunoglobulin (Molecular Probes, A21202 ...
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bioRxiv - Plant Biology 2021Quote: ... Rabbit polyclonal anti-AtTET8 (Homemade) and normal rabbit immunoglobulin G (Thermo Fisher), were coated with protein A beads in IP buffer (20 mM MES hydrate ...
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bioRxiv - Pathology 2020Quote: ... or HRP-conjugated goat anti-rabbit immunoglobulin (1:2000 dilution, Thermo Scientific) were used to detect specific protein bands (as noted in the figure legends ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa 594-conjugated goat anti-hamster immunoglobulin (Invitrogen, A-21113, 1:1000) was used as a secondary antibody for Podoplanin detection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa 488-conjugated donkey anti-rat immunoglobulin (Invitrogen, A-21208, 1:1000) was used as a secondary antibody for CD41 and CD44 detection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa 488-conjugated goat anti-rabbit immunoglobulin (Invitrogen, A-11034, 1:1000) was used as a secondary antibody for Clec-2 detection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Alexa 594-conjugated goat anti-rabbit immunoglobulin (Invitrogen, A-11012, 1:1000) was used as a secondary antibody for Chi3l1 detection ...
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bioRxiv - Microbiology 2023Quote: ... Polyclonal goat anti-pig immunoglobulin G (IgG) Fc Secondary Antibody HRP (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... followed by Alexa Fluor 488–conjugated goat anti-rabbit immunoglobulin (Life Technologies). Cells were stained with propidium iodide (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... The expression vectors encoding mHVEM (Q39-Q206) fused with human IgG1 and a subsequent hexa-His tag sequences were transfected into Expi293 (Gibco) cells using ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The DNA sequences encoding a protein biologic composed of mHVEM residues (Q39- Q206) followed by human IgG1 and a subsequent hexa-His tag sequences were cloned into pcDNA 3.3 vector (Life technologies) using In-fusion HD cloning enzyme premix (Clontech) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... Human T cells were activated with human CD3/CD28 Dynabeads (Invitrogen), at a bead:cell ratio of 2:1 and transduced using supernatant collected from Phoenix-AMPHO cells ...
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bioRxiv - Microbiology 2020Quote: ... Tsp-his was detected with the Anti-6X His tag antibody (ThermoFisher Scientific), which is already conjugated with HRP and does not require a secondary antibody for detection ...
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bioRxiv - Developmental Biology 2023Quote: ... For antibodies we used a His antibody (6x-His Tag Monoclonal Antibody, ThermoFisher), a MBP antibody (Anti-MBP Monoclonal Antibody ...
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bioRxiv - Cell Biology 2024Quote: ... 500 μg of protein lysates were incubated with 5 μg of His-RILP (NovoPro Labs #512098) or His-Myc using His Pull-down kit (Thermo Scientific #21277) for 2 hours at 4 °C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... For both fungi, first we obtained fresh blastospores (i.e., yeast-like single cells) suspended in Grace’s Insect Medium (Gibco, Thermo Fisher Scientific) supplemented with 2.5% FBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells expressing ACE2 receptors were suspended using TrypLE Select Enzyme solution (Thermo Fisher Scientific) and immediately added to all wells (10,000 cells in 100 μl of growth medium per well) ...
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...