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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... remaining cells were seeded into a 12-well plate with 1 × 106 cells per well in 2 ml culture medium consisting of DMEM-GlutaMAXTM (Gibco, ref.no: 31966-021), supplemented with penicillin (50 I.U./mL) ...
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bioRxiv - Synthetic Biology 2023Quote: ... supernatant was harvested from 293S GnTi- TetR sender A’-C (senders 1) and 293S GnTi- TetR sender Γ’-A’ (senders 2) cells that have been continuously cultured in DMEM (41966; Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... The transfection experiments were performed with pGL3 luciferase reporter plasmids separatly and in co-transfection with the pDiPRO1 vector using a 1:2 ratio of DNA (µg) to Lipofectamine™ 2000 transfection reagent (µl) (Invitrogen, Life Technologies). Myoblasts and RMS cells were seeded on 24-well culture vessels at 3.8×104 cells per well the day before transfection ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify N gene of SARS- CoV-2 using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described (73 ...
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bioRxiv - Cell Biology 2023Quote: ... Click-iT reaction buffer additive 1 and Click-iT reaction buffer additive 2 from the Click-iT™ Protein Reaction Buffer Kit (Thermo Fisher Scientific) were added to the sample according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Cells were then plated onto Geltrex-coated surfaces at 1.5×105 cells/cm2 in the induction medium (DMEM/F12 supplemented with 1× N-2 (Thermo Fisher Scientific, cat# 17502048), 1× non-essential amino acids (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Molecular Biology 2023Quote: ... 25 µg of peptides were applied onto an Acclaim PepMap 100 C18 column (2 μm particle size, 1 mm ID × 150 mm, 100 Å pore size; Thermo Fisher Scientific) and separated using a two-stepped gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA concentrations of 30 samples were normalized to approximately 15 ng µl-1 and TSV was detected and quantified from 2 µl of sample using a TaqMan™ Fast Virus 1-Step Master Mix (Applied Biosystems™) following the OIE recommended protocol and triplicate standards (mean R2 = 0.93 ± 0.06 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Biochemistry 2023Quote: ... Every well contained a 25 µL solution of 0.4 mg/mL of enzyme (with or without 2 mM of ADP-ribose) and SyproOrange (ThermoFisher; at 1:5000 dilution) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... for 2 hours at RT and then in the blocking buffer containing monoclonal anti-GFP mouse antibody (GF28R, 1:3000, Thermo Fisher, #MA5-15256) and biotin-conjugated anti-ALFA nanobody (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich], and 0.05% Tween 20 [Thermo Fisher Scientific]) was added and plates incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The attached corneocytes were infected with 250μl bacterial suspension at 2×107 CFU/ml in CO2 independent media supplemented with 10% FBS and 1% GlutaMax™ (Thermo Fisher Scientific). After 3h incubation at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... sections were incubated for 2 h at room temperature with fluorescently conjugated secondary antibodies: Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen A11008, 1:200) or Alexa Fluor 647 goat anti-mouse IgG (Invitrogen A21235 ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: ... and reduced for 30 min at 37°C with 1:125 dilution of Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (ThermoFisher, Waltham, MA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... cell nuclei were stained with DAPI diluted to 1:5000 in PBS 1X for 2 minutes at RT in the dark (Life technologies, Fischer Scientific). Slides were finally mounted on coverslips and the Fluoromount-G mounting media (Southern Biotech) ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: We mixed 2 μl of EEEV VLP at a concentration of 1.6 mg ml-1 in DPBS (Thermo Fisher Scientific Cat #: 14190-144) with 2 μl of VLDLRLBD-Fc in TBS supplemented with 2 mM CaCl2 at a concentration of 1.6 mg ml-1 ...
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bioRxiv - Immunology 2023Quote: Ex vivo isolated and rested CD8+ T cells or transduced T cells from splenocytes were incubated with R10 (RPMI 1640 + 10% FBS + 1% penicillin/streptomycin + L-glutamine + 20 U/ml of IL-2; Gibco, Thermo Fisher Scientific) alone or R10 containing PMA/ionomycin for two hours ...
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bioRxiv - Developmental Biology 2023Quote: ... lungs were minced with forceps and transferred to a 5 ml glass tube and crosslinked with 2 ml of 1% formaldehyde diluted with PBS from 10% buffered formalin (i.e. 3.7% formaldehyde;Thermo Fisher Scientific, 23-245-685) for 10 min at room temperature on a rocker ...
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bioRxiv - Neuroscience 2023Quote: ... magnetic beads (30 µL) were incubated at room temperature for 2 hours with 1 µg mouse monoclonal anti-GFP (Invitrogen 3E6 A-11120), washed thrice with Wash Buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Biophysics 2024Quote: ... Elution fractions were dialyzed overnight in 1 x PBS and 2 mM EDTA using 10 kDa MWCO dialysis cassettes (Cat # 66380, ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times in PBS and incubated for 2 hours in a goat anti-rabbit Alexa Fluor 555 antibody (A21429, 1:500, Invitrogen, Carlsbad, CA, USA), they were washed in PBS three more times ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) from donor # 1 and donor #2 were isolated and reprogrammed into iPSCs using the Sendai Reprogramming Kit (Thermo Fisher, cat # A34546). CFLine#1 was generated from donor #1 while CFLine#2a and CFLine#2b were derived from donor # 2 ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was blocked in NDS for 2 hr (RT) and then incubated in anti-rabbit MCHR1 antibody (1:3,000; Thermo Fisher Scientific; RRID: AB_2541682) prepared in NDS for 48 hr (4°C) ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was thoroughly rinsed with two exchanges in PBS (2 min each) then incubated with a donkey anti-rabbit Alexa Fluor 647 conjugate (1:500; ThermoFisher Scientific; RRID: AB_2536183) for 30 min (RT) ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary antibodies were incubated overnight at 4°C before a 2-h incubation with secondary antibodies (1:500, Invitrogen, #A21428 and #A21235) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 hours of incubation with secondary antibodies (1:500; Alexa Fluor 488, Alexa Fluor 568, Alexa Fluor 647; Molecular Probes, Thermo Fisher Scientific). DNA was stained with DAPI for 1 min ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated with secondary antibodies for 2 h (anti-rabbit in goat Alexa-Fluor 488 nm, 1:500, Invitrogen Thermo Fisher Scientific, USA). For the second set of slices ...
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bioRxiv - Systems Biology 2023Quote: ... Followed by a quick rinse in distilled water and a 2-minute rinse in 1% acetic acid solution (Fisher Scientific, cat# A38S-500). After dehydration with Xylene ...
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bioRxiv - Microbiology 2022Quote: ... was generated using 1-2 μg of RNA as a template for the SuperScript IV Reverse Transcriptase (RT) VILO master mix (Invitrogen, Thermo Fisher Scientific) with a no-RT control reaction in a half-reaction volume following manufacturer protocols ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes of the sample were loaded onto a 12% polyacrylamide gel and electrophoresed at 100 volts for 1 hour before being transferred to a PVDF membrane using the iBlot 2 system (Thermo Fisher Scientific IB21001). Membrane blocking was performed at room temperature in 3% nonfat dried milk in PBST for 1 hour before incubating overnight with primary antibodies (1:1000 in PBST ...
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bioRxiv - Cell Biology 2022Quote: ... Breg adenovirus-transduced brown adipocytes were cultured in serum-free medium with 1× protein transport inhibitor cocktail (10.6 µM Brefeldin A and 2 µM Monensin) (Fisher scientific, #50-930-9), 10 µM oligomycin ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Immunology 2022Quote: ... were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024], 1 mM sodium pyruvate [Gibco Life Technologies, 11360-039] ...
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bioRxiv - Cell Biology 2022Quote: All PURE translation reactions were carried out for 2 h at 32°C and then ribosome-associated nascent chains were released by addition of 1 mM puromycin (Thermo Fisher Scientific, USA) and further incubation for 10 min at 32°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 35ul of Dynabeads Protein A is taken for each sample for immunoprecipitation and these beads are once washed with 1×PBS and then allowed to incubate with 2% BSA and 10ng/ml Yeast tRNA (Thermo Scientific™, AM7119) for blocking the beads to avoid non-specific binding of proteins and RNAs ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Genetics 2023Quote: Genomic DNA from cells was isolated using a lysis buffer (10 mM Tris-HCl, 2 % Triton™ X-100, 1 mM EDTA, proteinase K [20 mg/mL]; Thermo Fisher Scientific). Cells were lysed at 60°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey anti-rat 555 secondary antibody (ThermoFisher, A48270, 1:1000), donkey anti-guinea pig 647 secondary antibody (Biotium ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-DG was solubilized as a 1 M stock solution in DMEM medium (without glucose, pyruvate and glutamine, Gibco, cat. no. A14430-01) and used at 10 mM ...
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bioRxiv - Microbiology 2024Quote: ... The upper and lower conjunctiva from both eyes were collected in 2 mL microcentrifuge tubes with 1 mL of RNA Later (Invitrogen, Waltham, MA, USA) and stored at −80°C for RNA extraction ...
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bioRxiv - Cell Biology 2024Quote: ... Testicular tissues were cut into small pieces of 1–2 mm and digested in 10 mL DMEM: F12 media (Gibco, Waltham, MA, USA) containing 1 mg/mL type IV collagenase for 15 min on a rotor at 37°C ...