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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and RNA integrity was estimated by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at −80⁰C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated for with varying concentrations of BCNU (0 to 2 mM) for an additional 48 h before incubation with Calcein AM dye (2 μM) (Invitrogen; Cat# C1430, Grand Island, NY) at 25°C for 30 m ...
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bioRxiv - Genomics 2021Quote: ... RNA Polymerase II 8WG16 (Covance #MM2-126R, 2 µg/ChIP) and Phospho-RNA pol II CTD (Ser5) (Thermo Fisher Scientific #MA1-46093, 2 µg/ChIP). ChIP assays for qPCR quantification were performed using primers shown in Table 2 ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed for 2 genes of interest and 2 internal controls (Suppl Figure S1) using Taqman® gene expression assays (Thermo Fisher, Foster City CA) and Taqman® Fast Universal Master Mix (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... Cells were starved in FCS-free DMEM for 2 hours prior to experiments and labeled during the last 20 minutes of starvation with 2 µM CellTracker™ Red CMTPX and 2 µg/ml Hoechst 33342 (both from Molecular Probes, Eugene, OR, USA). A gap was made in the cell monolayers of every well using an HTS Scratcher (Peira ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was determined by separating 2 μL of each sample on a 2% agarose gel and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA) and stored at -80°C until further use ...
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bioRxiv - Immunology 2020Quote: ... were calculated using RQ-Min = 2-(ΔΔCt + T *SD(ΔCt)) and RQ-Max = 2-(ΔΔCt - T *SD(ΔCt)) from triplicate wells as suggested by Applied Biosystems (manufacturer of qPCR machines). Results shown are representative of multiple independent experiments with similar results.
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bioRxiv - Microbiology 2024Quote: ... for 2 min before separation on an Easy-Spray™ C18 nano column (75 µm x 150 mm, 2 µm, Thermo Fisher Scientific, Waltham, MA) with a 107 min gradient at a flowrate of 0.3 µL/min ...
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bioRxiv - Neuroscience 2024Quote: ... To precipitate the RNA, 250 μL of 2-propanol (AppliChem, Darmstadt, Germany) and 2 μL of GlycoBlue Coprecipitant (15 mg/mL) (Thermo Fisher Scientific, Waltham, MA, USA) were added to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 1:125 TMRE (tetramethylrhodamine ethyl ester) (10µM) (Invitrogen™, cat. No. T669) for 30 minutes at 37°C in PBC + 1% FCS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2h with a first secondary antibody (rabbit Alexa Fluor-488–conjugated, 1:500, Invitrogen, Canada). A blocking step of antigenic sites from the first primary and secondary antibody combination was performed thereafter by a 3h incubation with normal serum from the same species as the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation in secondary antibody (goat anti-mouse Alexa 647, 1:200, Invitrogen A-21236). Slices were then mounted and scanned on a Leica TCS SP8 STED 3X confocal with white light laser and imaged with a 40X/1,3 NA oil immersion objective ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated for 2h at room temp with Alexa Fluor-conjugated secondary antibodies (1:400; Molecular Probes) and counterstained with phalloidin for F-actin (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Neuroscience 2022Quote: ... ethyl alcohol (Fisher Scientific) solution for four days to remove lipids from the sections ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then washed 3 times in PBS and incubated with appropriate fluorochrome secondary antibody diluted in blocking solution for 1-2h (AlexaFluor® 488, AlexaFluor® 568, 1:400, Life Technologies). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and HER-2 negative 24JK sarcoma cells were labelled with 5 mM or 0.5 mM CellTrace™ Violet (CTV; Invitrogen-Molecular Probes) respectively in D-PBS supplemented with 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: Proliferation was assessed through incorporation of 5-ethynyl-2’-deoxyuridine (EdU) using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, C10425). One day prior to harvest ...
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bioRxiv - Cell Biology 2020Quote: ... Fixation for 5 min at room temperature was performed by adding an equal volume of PBS 2% methanol-free formaldehyde (Thermo Scientific, USA ...
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bioRxiv - Genetics 2021Quote: ... and primers specific for target and control regions (in which the mark is expected to be enriched (positive controls) or depleted (negative controls)) (Table 2) using the QuantStudio 5 (Applied Biosystems) thermal cycler ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were subject to local UVC irradiation at 500 J/m2 and incubated with 20 μM 5-ethynyl-2-deoxyuridine (EdU, ThermoFisher Scientific) and 1 μM 5-Fluoro-2’-deoxyuridine (FUdR ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-ethynyl-2′-deoxyuridine (EdU) incorporation was detected using a Click-iT Plus EdU Alexa Fluor 555 Assay Kit (Invitrogen, C10638) prior to primary antibody incubation ...
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bioRxiv - Cell Biology 2020Quote: ... Differentiation was stimulated by replacing the proliferation medium with differentiation medium (high glucose DMEM with sodium pyruvate, 5% horse serum, 2% L-glutamine, ThermoFisher Scientific). The cells were maintained at 37°C and 5% CO2 throughout differentiation.
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bioRxiv - Genomics 2020Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 μm × 2 cm, nanoViper, C18, 5 μm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 μm × 50 cm ...
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bioRxiv - Biochemistry 2020Quote: ... targeting the TLR3 gene (5’-CCUGAUGAUCUUCCCUCUAACAUAA-3’) and Stealth RNAi siRNA negative control med GC Duplex #2 were obtained from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were loaded on to 2cm trap column (C18, 5 μm, 100 Å, 100 μm × 2 cm; PN: 164562, Thermo Scientific) and further loaded on 50 cm Easynano spray analytical column (2 μm ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos or ovaries dissected from 5 well-fed females per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μl TURBO DNase (2 U/μl ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap C18, 5 μm, 100 μm × 2 cm, Thermo Fisher Scientific) connected to an analytical column (Acclaim PepMan 100 ...
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bioRxiv - Plant Biology 2020Quote: ... and a final extension of 5 min at 72°C. The target band (approx. 100 bp) was extracted from a 2% E-Gel (Thermo Fisher). For multiplex sequencing ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then stained with DAPI (2 ug/mL) for 5 minutes before mounting with ProLong Diamond antifade mountant (Thermo Fisher).
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bioRxiv - Systems Biology 2020Quote: ... Peptides were trapped at 20 μl/min in loading solvent (2% acetonitrile, 0.05% TFA) on a 5 mm x 300 μm C18 pepmap cartridge pre-column (Thermo Fisher Scientific) during 5 minutes ...
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bioRxiv - Microbiology 2020Quote: HeLa cells (ATCC CCL-2) were incubated in RPMI (Roswell Park Memorial Institute) medium containing 5% FCS (fetal calf serum, Gibco®) in an incubator with 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... digests were reconstituted in 10 μL of 0.3% TFA in 2/98 MeCN/H2O and 5 μL were analyzed by LC-MS/MS using an RSLCnano system (Ultimate 3000, Thermo Scientific) interfaced on-line to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Pregnant female mice received intraperitoneal (i.p.) injections with 50µg of 5-ethynyl-2’-deoxyuridine (EdU)/g body weight (Thermo Fisher Scientific – A10044), 16 hours prior to tissue collection ...
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bioRxiv - Microbiology 2020Quote: ... and purified RNA from 2 ml of cells harvested immediately before and 5 min after stress using the RiboPure™-Bacteria Kit (Ambion), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptides were loaded onto a trap column (Acclaim C18 PepMap nano Trap x 2 cm, 100 μm I.D, 5 μm particle size and 300 Å pore size; ThermoFisher Scientific) at 15 μL/min for 3 min before switching the pre-column in line with the analytical column (Acclaim RSLC C18 PepMap Acclaim RSLC nanocolumn 75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was removed from the antibody by boiling at 100 °C for 5 min in 2 X LDS Sample buffer (Life Technologies). An equal volume of each sample and its corresponding input was loaded onto a gel for electrophoresis and western blot analysis.
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bioRxiv - Cell Biology 2021Quote: ... GFP-associated or immunoprecipitated complexes were eluted (30 μl) at pH 2 for 5 min at 25°C and neutralized according to the manufacturer’s instructions (88805; Thermo Fisher Scientific). Samples were then reduced at 70°C for 5 min by dilution in 5x sample buffer (125 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... the samples were loaded via an Acclaim PepMap 100 trap column (100 µm x 2 cm, nanoViper, C18, 5 µm, 100å; Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Microbiology 2022Quote: ... blotted for 5 s using blot force 2 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific). The data were collected on a Thermo Scientific™ Krios™ G4 Cryo Transmission Electron Microscope (Cryo-TEM ...
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bioRxiv - Molecular Biology 2022Quote: Visualisation of Fasciola proliferative cells from 21 day old juveniles maintained in vitro and in vivo was achieved by labelling nuclei undergoing DNA-synthesis with 5-ethynyl-2-deoxyuridine (EdU; ThermoFisher Scientific) as described by [15] ...
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bioRxiv - Cell Biology 2022Quote: ... cells were passaged at day 5 of differentiation into 2-well chamber slides for the remainder of hepatic directed differentiation (ThermoFisher Scientific). For mitochondrial morphology analysis MitoTracker Deep Red FM (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with 0.5 µM Lipi-Deep Red neutral lipid stain (Dojindo #LD04-10) for 2 hr and 5 µg/mL Hoeschst 33342 nucleic acid stain (Invitrogen #H3570) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... and a goat anti-mouse antibody conjugated with Alexa Fluor 488 (Life Technologies; 2 μg/ml in ND96 with 5% BSA) was applied for 1h at RT ...