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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Four microliters RNA was used for real-time RT-qPCR to detect and quantify genomic RNA of SARS-CoV-2 N gene using TaqMan™ RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) as described2 using the following primers and probes ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used as input with 2 μL of a 1:100 dilution of ERCC RNA Spike-In Mix (ThermoFisher Scientific, catalog 4456740) and the KAPA RNA Hyper + RiboErase HMR kit was used (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... 2-DG was solubilized as a 1 M stock solution in DMEM medium (without glucose, pyruvate and glutamine, Gibco, cat. no. A14430-01) and used at 10 mM ...
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bioRxiv - Microbiology 2024Quote: ... The upper and lower conjunctiva from both eyes were collected in 2 mL microcentrifuge tubes with 1 mL of RNA Later (Invitrogen, Waltham, MA, USA) and stored at −80°C for RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 hours in either of the following secondary antibodies: Alexa Fluor 594 goat anti-rabbit (Invitrogen, 1:300, A11032), and Alexa Fluor 594 goat anti-rat (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation for 2 h in a donkey anti-rat secondary antiserum (1:750; AlexaFluor-594, Thermo Fisher Scientific, catalog #A-21209). Last ...
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bioRxiv - Synthetic Biology 2024Quote: ... Overnight cultures were used to inoculate 300 µL volumes of MOPS EZ Rich media with 2% glucose and 100 mM L-Thr at a 1:100 inoculation ratio in a 96-deep-well plate (Thermo Scientific™ 260251) with appropriate antibiotic (30 µg/mL Kan) ...
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bioRxiv - Immunology 2024Quote: Cells were seeded in bottom glass dishes and stained with nucblue (2 drops per 1 mL of NucBlue™ Live ReadyProbes™ Reagent (Hoechst 33342) ThermoFisher) and mitotracker (MitoTracker™ Deep Red FM, 2 μM ThermoFisher). Cells were acquired in a confocal microscope Leica SP8 and processed with the Leica Application Suite.
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bioRxiv - Cell Biology 2024Quote: ... Testicular tissues were cut into small pieces of 1–2 mm and digested in 10 mL DMEM: F12 media (Gibco, Waltham, MA, USA) containing 1 mg/mL type IV collagenase for 15 min on a rotor at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium was changed twice a week and spheroids were splitted every 1 to 2 weeks when reaching 800 to 1000 µm of diameter using TrypLE Express Enzyme (Thermo Fisher Scientific, 12605010) preheated to 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Plant Biology 2024Quote: ... One tank was treated with a mock solution while the other tank was treated with a 1 M solution of 2-aminoisobutyric acid (AIB) (Fisher Scientific, Cat. AAA1302114) to a final concentration of 10 mM ...
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bioRxiv - Neuroscience 2024Quote: ... slices were washed for 15 min three times with 1XPBS and then incubated for 2 hr at room temperature in dye-conjugated secondary antibodies (1:1000; Invitrogen, Grand Island, NY) including BV480 (goat anti-rat)Alexa Fluor 488 (goat anti-chicken) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 2-hour incubation at RT with secondary antibodies: Alexa Fluor 594 goat anti-mouse (1:500, Thermo Fisher, A-11005) and Alexa Fluor 488 goat anti-rabbit (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Around ten µg of peptides were applied onto a commercially available Acclaim PepMap 100 C18 column (2 µm particle size, 1 mm ID × 150 mm, 100 Å pore size; Thermo Fisher Scientific) and separated using a stepped gradient with acetonitrile concentration ranging from 3% to 24% to 31% solvent B (0.1% FA ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cells were incubated with 2 µg mL-1 Hoechst 33342 for 45 min and the medium was changed for FluoroBrite DMEM (Gibco, Thermo Fisher Scientific) supplemented with 10% FBS and 1% Penicillin-Streptomycin ...
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bioRxiv - Physiology 2024Quote: ... were aspirated using a 21-gauge needle attached to a 5 mL disposable syringe in the presence of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-buffered TCM-199 medium (Gibco, Life Technologies, Milan, Italy) and 0.005% (w:v ...
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bioRxiv - Immunology 2024Quote: ... Clearing was initiated by incubating samples in a 1:1 mixture of methanol and BABB (benzyl alcohol and benzyl benzoate at a 1:2 ratio, Sigma ref. 305197 and Fisher Scientific ref. 10654752), followed by overnight incubation in pure BABB ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with one or more of the following secondary antibodies at room temperature for a minimum of 1–2 hours: Alexa-fluor 488 anti-mouse IgG (Invitrogen, Cat # A-32723), Alexa-fluor 555 anti-rabbit IgG (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The IVM medium consisted of 25 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid-buffered tissue culture medium 199 (M199; Gibco, Paisley, Scotland, UK) supplemented with 5% calf serum (CS ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer containing a protease inhibitor (ThermoFisher Scientific, Massachusetts, USA). The protein concentration was measured using the Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Passage 3 hMSCs from 4 donors chosen randomly (2 male; B, G, and 2 female; C, D) were thawed and cultured in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) supplemented with RoosterNourishTM-MSC expansion medium (RoosterBio ...
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bioRxiv - Plant Biology 2020Quote: ... The RNA was treated with 2 U DNase I (Life Technologies) for 30 min at 37°C and cleaned using the RNeasy Plant Mini kit (Qiagen) ...
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bioRxiv - Biophysics 2021Quote: ... Nucleus were stained with DAPI (4’, 6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... after 2 days of 1000 ng/mL doxycycline induction (Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... after 2 days of 1000 ng/mL doxycycline induction (Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... 2 ml Trypsin-EDTA (0.25%; Gibco, Thermo Fisher Scientific, Massachusetts, USA) was added and the flask was incubated at 37°C for 2-3 minutes to detach the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... and then transfected using 2 µL of Lipofectamine 2000 (Life Technologies) and 1.6 µg of DNA per well with the GFP-LC3B expression plasmids with or without scrambled shRNA or a mixture of five FYCO1-targeting shRNAs (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... For Glucose uptake analysis 2-NBDG glucose-uptake marker (Thermofisher Scientific) was used ...
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Superresolution microscopy reveals photoreceptor-specific subciliary location and function of Cep290bioRxiv - Cell Biology 2020Quote: ... F(ab’)2-goat anti-rabbit IgG Alexa 555 (Thermo Fisher) was used for dual labeling ...
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bioRxiv - Cell Biology 2020Quote: ... After selection with 2 mg/ml G-418 (Thermo Fisher Scientific), the resulting clones were screened under a fluorescence microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were eluted in 2× NuPAGE LDS Sample Buffer (Life Technologies) by boiling at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... supplied with 2 μg MS grade Trypsin (Thermo Fisher Scientific, 90057) and incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... section were incubated with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS for 10 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were stained with 2 μg/μl Hoechst 33342 (Invitrogen H3570) in PBS for 10 minutes at RT ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was stained with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). After washing with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; Invitrogen). ProLong Gold Antifade Mountant (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... before incubation in Digestion Buffer containing 2 mg/ml Collagenase (Gibco) and 0.5 mg/ml hyaluronidase (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... iPSCs were dissociated with 2 mg/ml collagenase IV (Thermo Fisher) for 45 min at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Alexa-Fluor-555-conjugated streptavidin (Thermo Fisher #S21381, 2 µg/ml) was used for 1 hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... with 10% FBS (Biological Industries) and 2 mM GlutaMax™ (Gibco). T2 cell (ATCC ...
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bioRxiv - Microbiology 2020Quote: ... RNA (∼3 µg) was treated with 2 units turbo-DNase (Invitrogen) in 1X turbo-DNase buffer for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Tissue was treated with 2 ml of Trypsin-EDTA 0.05% (Gibco) at 37°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 5M NaCl and 2 μL RNAse A (Thermo Fisher Scientific, #EN0531). The samples were incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were stained with 2 µM BODIPY 581/591 C11 (Invitrogen) in standard medium and incubated at 37 °C for 60 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2 μL SuperScript III Reverse Transcriptase (Thermo Fisher Scientific, 18080044) were added into the above 15 μL reaction system and incubated under 50 °C for 50 min then 70 °C for 10min ...
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bioRxiv - Developmental Biology 2021Quote: ... the Mass spectrometer used was the QExactive Plus 2 (Thermo Scientific), the analytical column used was the Acclaim PepMap RSLC (75μm x 50cm ...