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Citations for Thermo Fisher :
9201 - 9250 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Samples were then resuspended in 300 μl PBS + 2% FBS together with 5 μl CountBright™ Absolute Counting Beads (ThermoFisher Scientific) and run on a 5-laser BD Fortessa (BD Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... The temperature was increased from 5 °C to 95 °C over 2 hours and readings taken using a One Step Plus RT-PCR (Applied Biosystems).
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Cell Biology 2023Quote: ... From day 0 to day 5 the medium was changed daily with 2 mL DMEM/F-12 containing 20% KnockOut™ Serum (ThermoFisher), 1 x Non Essential Amino Acid (NEAA ...
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bioRxiv - Genetics 2023Quote: ... Ovaries were then washed 3 times for 5 min with PBTx followed by a blocking step in PBTx supplemented with 2% normal goat serum (NGS, Invitrogen, ThermoFisher Scientific) and 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Assorted tissues from third instar larvae (L3 stage) of the wild type Canton-S strain were dissected during 2-5 min in PBS (Thermo Fisher) with or without 10 % glycerol after turning larvae inside out with the help of fine tip forceps ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylation and streptavidin-bead binding were performed as above with the exception that in this case the flowthrough was discarded and bead-bound nascent RNA was eluted by incubating the beads at 2 x 5 min at 65C in 100mM TCEP (Thermo Scientific), 10mM EDTA ...
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bioRxiv - Neuroscience 2023Quote: ... Two larvae at a time were cold anesthetized on ice in a custom recording chamber for 5 minutes and immobilized dorsal side up in 2% low melting point agarose (BP1260-100, Fisher Scientific). Recording chambers were placed on the stage of an upright microscope (Olympus BX-51W ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: Proliferative activity of 2D hydrogel cell cultures was measured using an EdU (5-ethynyl-2’-deoxyuridine) labeling kit (Click-iT™ EdU Cell Proliferation Kit for Imaging, Invitrogen). 5 μM EdU solution was dosed to cells 12 h prior to fixing with 4% paraformaldehyde ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were desalted on-line using a capillary trap column (Acclaim Pepmap100, 100 μm, 75 μm × 2 cm, C18, 5 μm (Thermo Scientific)) and then separated using a 180 min RP gradient (4–30% acetonitrile/0.1% formic acid ...
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bioRxiv - Neuroscience 2023Quote: ... tissues were placed in a digestion solution containing HBSS-glucose and 5 mM CaCl2 (Cat. #: 1564A930, Fischer Scientific) and a combination of collagenase II (2 mg/ml, Cat. #: 17101-015, Life Technologies) and dispase (5 mg/ml ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... 20 U/mL recombinant human IL-2 at 37°C and 5% CO2 for 24 hours in RPMI 1640 (Gibco, Canada) containing 100 Units of penicillin and 0.1 mg/mL of Streptomycin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... 50 μL lysate (∼25 ug total protein) was mixed with 2 μg 5’-biotinylated dsDNA probe and 50 μL streptavidin agarose resin (Thermo Scientific) in PBS containing protease inhibitors (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... was added per well and incubated for 2-5 minutes before adding 100 µL the ELISA stop solution (Thermo Fisher Scientific). Absorbance was measured on the Varioskan microplate reader at 450 nm (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 100% EtOH 2 min) and xylene (two 5 min incubations) and mounted using Fisher Chemical Permount Mounting Medium (Fisher Scientific SP15) and microscope cover glass (Fisher Scientific 12545F) ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... was prepared fresh with the 3’aminated 5’ caged oligo root carrying a terminal Cy3 dye (2 μM BL003 for the single-cycle experiment and 2 μM BL728 for the 4-cycle experiment) and a bifunctional crosslinker BS(5)PEG (Thermo Scientific) (5 μM in dimethylformamide) ...
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bioRxiv - Plant Biology 2024Quote: In situ hybridization experiments were performed on 2 - 5 mm ear primordia and 10-21 day old seedling apexes fixed in PFA (Thermo Scientific), dehydrated in a graded ethanol series ...
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bioRxiv - Neuroscience 2024Quote: ... the electrodes were filled with 2 µl of 5 mM of Alexa Fluor 568 hydrazide (Invitrogen, diluted in 200 mM KCl) and backfilled with 10 µl of 200 mM KCl ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... A small piece of renal CTX tissue (approx. 2 mm) and the renal IM were transferred to 5 ml ice-cold PBS (Thermo Scientific). After centrifugation at 500 x g for 5 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay was performed using Click-iT Plus EdU Alexa Fluor 488 Imaging Kit (Invitrogen, C10637). Animals were injected intraperitoneally with EdU reagent at a dose of 10 µl per 1 g animal (stock solution 20 mM solution in saline) ...
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bioRxiv - Molecular Biology 2024Quote: ... Trapping was performed at 20 μl/min for 2 min in loading solvent A on a 5 mm trapping column (Thermo scientific, Pepmap ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 2×5 min in TBS and cover-slipped with ProLong Diamond mounting media 275 containing DAPI (#P36962, ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the sample was loaded on an Acclaim PepMap 100 trap column (100 µm × 2 cm, C18 reversed phase, particle size: 5 µm; Thermo Scientific) using a flow rate of 7 µL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed in TSB-T 2 x 5 min and rinsed with TBS before mounting with ProLongTM Diamond Antifade Mountant (Invitrogen, #P36961). Following staining with secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... Dried cell lysates were digested using 2 μL of a digestion solution containing 5 ng/μL LCMS grade trypsin (Pierce) in 0.1% DDM (Thermo Fisher, 89902) and 50mM TEAB (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... the nucleus was counterstained with 0.5 µg/mL 4’6-diamidino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Prolong® Gold (Life Technologies).
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bioRxiv - Molecular Biology 2024Quote: ... MDA-MB-231 and HEK-293T RNAs (5 μg) were incubated with 2 U TURBO DNase for 30 min at 37 °C (Thermo Fisher). After DNase digestion ...
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bioRxiv - Cell Biology 2024Quote: ... Cells obtained from fractions 2–5 were combined according to the genotype and expanded in α-MEM containing 10% FBS (Gibco), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2024Quote: ... 400,000 cells were stained with 2 mL of 5 µM C11-Bodipy 581/591 fluorescent probe in Live Imaging Solution (Thermo Fisher) or left unstained (unstained control group) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The sample was then dialyzed for 5 hours in 10x PBS pH 8.4 buffer with a 2 kDa membrane (Fisher Scientific, #15310692) and quantified with a NanoDrop ...
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bioRxiv - Physiology 2024Quote: Detached corneal epithelial sheets and dissociated cells were placed in the recording chamber (Warner Instruments (Hamden, CT, USA)) and loaded with Fura-2 AM (5-10 µM, Life Technologies) for 30 to 45 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... IP samples used for the broad peptide screen in Figure 2 were prepared using 100 mg HEK293TLD / pLD401 powder and 5 µl (slurry) Dynabeads M-270 Epoxy (ThermoFisher Scientific) conjugated with α-ORF2p cl ...
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bioRxiv - Molecular Biology 2024Quote: Differentiated hiPSCs-derived control myotubes or human primary control myoblasts were transfected at day 5 of differentiation with 50 nM 2-OMe ASOs using RNAi Max transfection reagent (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... qRT‒PCR was performed using 2× RealStar Power SYBR qPCR Mix (Genstar #A304) in a QuantStudio 5 Real Time PCR system (Applied Biosystems). cDNA was synthesized using a StarScript III RT Kit (Genstar #A232) ...
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bioRxiv - Biochemistry 2024Quote: Cells were treated as described in the figure legends and 1 hour prior to harvest incubated with 10μM 5-ethynyl-2-deoxyuridine (EdU; Click-iT EdU Flow Cytometry Kit, ThermoFisher, Loughborough, UK). Cells were harvested by trypsinisation and fixed with 4% paraformaldehyde/PBS for 10 min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... cells were beadloaded with 6 µL of 5 µM probe and 100 nM HaloTag-JF669 ligand and 2 drops of NucBlue live cell stain (Life Technologies) were added at the time of beadloading to stain the HaloTag-G3BP1 protein and illuminate the nucleus ...
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bioRxiv - Cancer Biology 2024Quote: 293FT cells cultured in antibiotic-free media (DMEM, 10% FBS, 2 mM glutamine) were transfected in 5% Lipofectamine™ 2000 (Invitrogen) using packaging plasmids Δ8.9 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 μL of 5 fold diluted cDNA product was subjected to RT-qPCR in StepOnePlus Real-Time PCR System (Applied Biosystems) using SYBR Green PCR Master Mix reagent as the detector ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were trapped at 20 μL/min in a loading solvent (2% ACN, 0.05% trifluoroacetic acid) on a 5 mm × 300 μm C18 PepMap cartridge pre-column (Thermo Fisher Scientific) for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked with TBST + 5% nonfat dry milk then immunoblotted with anti-alpha-actinin 2 (Invitrogen EA-53; mouse monoclonal) and anti-actin (GeneTex HL1926 ...
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bioRxiv - Bioengineering 2024Quote: ... media containing lentivirus was replaced with fresh media and cells were allowed to recover for another 24 hours before starting selection with 2 μg/mL puromycin for 5 days (Gibco, A1113803).
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bioRxiv - Cell Biology 2024Quote: HEK-293T cells (5×105) were seeded in 2 wells of a 6-well plate in DMEM (Thermo Fisher Scientific #12430054) supplemented with 10% fetal bovine serum (GeminiBio #100-106) ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed 3 times in washing solution (PBS containing 0.05% Triton X-100) and incubated for 2 hours at room temperature in the secondary antibody and DAPI (Invitrogen, D3571, dilution 1:1000). The slides were afterwards washed 3 times in washing solution ...