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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... a Cy-3 conjugated donkey anti-guinea pig antibody was used (Molecular Probes). Fab-specific secondary antibodies were used for detection of VEGFR1 ...
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bioRxiv - Neuroscience 2024Quote: ... fresh culture medium containing 33μg/mL 3 kDa dextran-AlexaFluor 488 (Invitrogen; D34682), 33 μg/mL 10 kDa dextran-AlexaFluor 647 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... was used to carry out the qPCR in a QuantStudio 3 (Applied Biosystems). Reaction was setup as follows 10 μL of TaqMan fast advanced MM ...
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bioRxiv - Neuroscience 2024Quote: ... 3 nights) and used tyramide signal amplification (Tyramide Super Boost kit from Invitrogen). After blocking non-specific binding with 10% goat serum and then any endogenous peroxidase activity with 3% H202 ...
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bioRxiv - Neuroscience 2024Quote: ... A single drop of CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher R37111) was added to individual dishes and incubated at at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... Subsequently myotubes were washed 3 times with pre-warmed DPBS (A12856-01, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 3 μg total DNA was added to 100 μl Opti-MEM (Gibco, 31985047) and mixed ...
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bioRxiv - Cancer Biology 2024Quote: ... Following fixative treatment then staining with the Hema 3 kit (Thermo Fisher Scientific), quantity of cells that invaded through the VitroGel and across the Boyden chamber membrane was quantified ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then treated with 3 µl Turbo DNAse (ThermoFisher, Waltham, MA, USA) in a 50 µl reaction volume ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated in 3-12% Bis-Tris Native gel (Invitrogen, cat. BN1001BOX) and then transferred to PVDF membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 3 cycles of sonication in a water bath sonicator (Fisher Scientific) with each cycle consisting of 30 s of sonication followed by 2 min in an ice bath.
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bioRxiv - Microbiology 2024Quote: ... The cell pellet was resuspended in 3 ml of ACK lysis buffer (Gibco) to lyse the RBCs ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was carried out on a QuantStudio 3 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... and loaded onto 3-8% gradient NuPAGE Novex Tris-acetate gels (Life Technologies). Electrophoresis was performed at 150 V for 4 h at 4°C in native gel running buffer ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was carried out on a QuantStudio 3 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: ... All experiments were performed on a QuantStudio 3 Real-Time PCR (ThermoFisher Scientific) and the raw fluorescence data was fit using a Boltzmann function to calculate the melting temperature (Tm ...
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bioRxiv - Bioengineering 2024Quote: ... Gene expression analysis was performed using QuantStudio 3 real-time PCR system (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... as was 7- Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen™ D346) and CPM stock was prepared at 5 mg/mL ...
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bioRxiv - Biophysics 2024Quote: ... and then cells are counted using a Countess 3 Automated Cell Counter (ThermoFisher). A cell suspension with a concentration of 15,000 cells/mL is made and 4 mL of the cell suspension is added to each well containing a coated CYTOO chip ...
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bioRxiv - Developmental Biology 2024Quote: ... containing appropriate 3’UTRs and 30nM microRNA mimic in 96-well plates (Invitrogen, miR-92b-3p #4464066 ...
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bioRxiv - Bioengineering 2024Quote: ... Hypersil GOLD™ C18 column (4.6 ∼ 150 mm, 3 μm, Thermo Scientific, USA) with a temperature of 30 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... siDLK1-3: #4392420 ID s16739) and control siRNA (#4390843) were purchased from Invitrogen. CU-ACC1 (5 x 105 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... Transduced cells were selected with 3 µg/mL puromycin (Thermo Fisher Scientific #A1113803) for K562 cells and 2 µg/mL for BHK-21 cells beginning two days after infection ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from cells 3 days after transfection using TRIzol (Thermo Fisher), and qPCR was performed to confirm EIF4EBP1 gene knockdown using TaqMan Assays (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... and sgRNAs at a 1:3 molar ratio in Neon buffer R (Invitrogen) and incubating at room temperature for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed for 10 mins using 3% PFA (Thermo Scientific, Cat: 28906) followed by 3X 5 mins washes with 1mg/ml NaBH4 in PBS to quench free aldehyde groups followed by three washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue DHPE) was purchased from Invitrogen. Each lipid was dissolved in chloroform within a round-bottom glass tube at a concentration of 20 mM ...
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bioRxiv - Cancer Biology 2024Quote: ... and PC-3 cells were maintained in RPMI medium (Invitrogen, Carlsbad, CA, USA). MDAPCa2b and DU145 cells were maintained in F-12K medium (ATCC ...
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bioRxiv - Genetics 2024Quote: ... 15 µg protein was loaded on 3-12% Bis-Tris NativePAGE gels (Invitrogen) and run according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Reactions were run on a QuantStudio 3 Real Time PCR instrument (Thermo Fisher) and analyzed using the ΔΔCT method (22) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Cell concentration was roughly assessed using the Countess 3 Automated Cell Counter (Invitrogen). For two out of the three Astroides samples ...
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bioRxiv - Cell Biology 2024Quote: ... and mRNA expression was quantified using the QuantStudio™ 3 System (Applied Biosystems). Gene expression was normalized to Hprt ...
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bioRxiv - Cancer Biology 2021Quote: ... platform and 80 samples were analyzed using GPL570 (Affymetrix Human Genome U133 Plus 2.0 Array) platform ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-human CD117 known also as cKIT (12-1178-42, Thermo Fisher Scientific, 1:100).
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Immunology 2021Quote: The IL-2 ELISA was performed using the IL-2 Human ELISA Kit by ThermoFisher scientific according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The cells were then stimulated with 20 ng/mL of recombinant human IL-4 (Gibco) and 20 ng/mL of recombinant human IL-13 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... T cells were stimulated for 2 days with anti-human CD3/CD28 magnetic Dynabeads (ThermoFisher) at a bead to cell concentration of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary human and mouse fibroblasts were cultured in Minimum Essential Media (MEM, Thermo Fisher Scientific) supplemented with 15% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Epidermal sheets were cultured separately in RPMI with 10% human AB serum (Thermo Fisher Scientific) and 1% Penicillin/Streptomycin/Amphotericin B solution for 48 h ...
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bioRxiv - Neuroscience 2022Quote: Human α-syn fibrils were labelled with Alexa-488 succinimidyl esther (Thermo Fisher Scientific, A20000) following manufacturer’s instructions and the unbound fluorophore was removed with multiple dialysis steps in sterile PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... human fetal lung tissues were dissociated with Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TIGIT PE Cy 7 (1:50, clone MBSA43, Invitrogen, cat # 25-9500-42), Anti-human CD94 APC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... human foetal lung tissues were dissociated using Dispase (8 U/ml Thermo Fisher Scientific, 17105041) at room temperature (RT ...
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bioRxiv - Cell Biology 2020Quote: ... was obtained from Abcam (Cat.# ab9498) (Cambridge, MA, USA) and anti-human ZO-1 antibody was obtained from Invitrogen / ThermoFisher (Carlsbad ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse and human sperm were loaded with Cal-520-AM (5 μM, Molecular Probes, USA) in the presence of Pluronic F-127 (0.02% v/v ...
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bioRxiv - Immunology 2021Quote: ... human cGAS was labelled with AlexaFluor-488 (AF488) carboxylic acid (succinimidyl ester) (Thermo Fisher Scientific) according to manufacturer’s manuals using a molar ratio of 1:10 at 4°C for 4 h ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral particles were produced in human 293T cultured in in Dulbecco’s Modified Eagle’s Medium (ThermoFisher), supplemented with 10% Fetal Bovine Serum (EuroBio ...
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bioRxiv - Bioengineering 2021Quote: ... We then stimulated T cells with Dynabeads™ Human T-Activator CD3/CD28 from ThermoFisher overnight with a ratio of beads to cells as 1:3 ...