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Citations for Thermo Fisher :
9301 - 9350 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using either Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... for LapA or 3 ul Spectra Multicolor High Range Protein Ladder (Thermo Fisher) for MapA ...
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bioRxiv - Cancer Biology 2023Quote: ... dried on 3 mm paper and mounted with Prolong Gold antifade reagent (Invitrogen). Imaging and image processing for fixed cells done in Fig ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was conducted on a Biosystems QuantStudio 3 system (Life Technologies) using 12.5 ng cDNA template ...
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bioRxiv - Cell Biology 2023Quote: ... and corresponding primers in the QuantStudio 3 Real-Time PCR System (Applied Biosystems) and analyzed using QuantStudio software (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using Accutase or ReleSR (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... Indole-3-carbinol (97%) and β-Naphthoflavone (98+%) were obtained from Thermo Scientific™ (122190250 and A18543.06 ...
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were then embedded in rat-tail collagen (3 mg/ml Life Technologies). Spheroids were then cultured with unconditioned media ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were run on NuPAGETM 3-8% Tris Acetate gels (Invitrogen EA0378BOX). To probe for CD63 ...
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bioRxiv - Microbiology 2023Quote: ... the cellular glyceraldehyde 3-phosphate dehydrogenase (GADPH) with SuperScriptTM III Reverse Transcriptase (Invitrogen) and quantified using SYBR-Green (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... 288 individual clones (3 x 96w plates) were selected using Collagenase (ThermoFisher Scientific) dissociation and manual selection under a microscope ...
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bioRxiv - Biochemistry 2023Quote: Native gel electrophoresis was performed using 3-12% Bis-Tris gels from Invitrogen and native running buffers from SERVA.
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bioRxiv - Cell Biology 2023Quote: ... and 3 µg of pSVGV packing vectors using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with hepatocyte wash medium (Thermo Fisher Scientific, 17704024). Cells were then plated in a 6-well plate precoated with collagen I (50µg/mL ...
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bioRxiv - Plant Biology 2023Quote: ... The qRT-PCR was performed in a Quantstudio 3 thermocycler (Thermo Fisher Scientific) using the SYBR select master mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Neuroscience 2023Quote: ... The calcium indicator was prepared by mixing 50 μg Fluo-3/AM (Invitrogen) with 50 uL DMSO and vortexed ...
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bioRxiv - Genomics 2023Quote: ... cells were pre-cultured for 3 days in phenol red-free DMEM (Gibco) supplied with 5% dextran-coated charcoal (DCC ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Immunology 2023Quote: ... S10-3 cells were grown in Dulbecco’s Modified Eagle’s Medium (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA amplification was performed for 40 cycles on a QuantStudio 3 (Applied Biosystems) and recorded with QuantStudio Design and Analysis Software ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using QuantStudio 3 (Thermofisher, A28567) with PowerUp SYBR Green Mastermix (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 times in PBS and mounted in Slowfade Gold mounting medium (Invitrogen). Primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged every 2-3 days using TryplLE™ express enzyme (GIBCO) and trypsin inhibitor (GIBCO).
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Neuroscience 2023Quote: ... After 3–4 days the colonies were dissociated using StemPro Accutase (Thermo Fisher), counted and plated at low confluency (10,000 cells/cm² ...
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bioRxiv - Genetics 2023Quote: ... each coverslip was incubated in 3 μl fura-2 acetoxymethyl (AM) ester (Invitrogen) in 1X HBSS containing 5 mg/ml BSA (Sigma- Aldrich ...
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bioRxiv - Genetics 2023Quote: ... We performed PCR using the QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific). We normalized transcript abundance to rp49 and calculated fold change via the ΔΔCt method (Rao et al. ...
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bioRxiv - Genomics 2023Quote: ... and the concentration was measured using a QUBIT 3 fluorometer (Thermo Fisher Scientific). DNA was then plated in 96-well plates following an order determined using the R package Omixer version 1.6.0 ...
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bioRxiv - Cell Biology 2023Quote: ... were transfected with 3 μg of DNA using the Neon electroporation system (Invitrogen), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: 5’ and 3’ RACE was performed using the GeneRacer kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... on a QuantStudio 3 real-time PCR system (Applied Biosystems, Thermo Fisher Scientific). The sequences of forward and reverse primers are available upon request ...
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bioRxiv - Cancer Biology 2023Quote: ... on a QuantStudio 3 real-time PCR system (Applied Biosystems, Thermo Fisher Scientific). The sequences of forward and reverse primers are available upon request ...
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bioRxiv - Cancer Biology 2023Quote: ... treatments were added in media containing 300 nm YOYO-3 (Thermo Fisher Scientific). Cells were cultured and imaged every 3 hours using an IncuCyte S3 (Essen Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... and siTACC3#3: D-004155-04-0005) using Lipofectamine 2000TM (Invitrogen, CA, USA) transfection reagent as described previously24 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected with 3 μg/ml puromycin (#A11138-03, Thermo Fisher Scientific). Single-cell clones that express similar TMEM192 levels were used in lyso-IP experiments.
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bioRxiv - Bioengineering 2023Quote: ... 330 µl of rat tail type-I collagen (3 mg/ml; Gibco A1048301) was gently diluted in 660 µl 1X PBS for a final collagen concentration of 1 mg/ml ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... Gene expression was quantified using QuantStudio 3 Real-Time PCR System (Applied Biosystems). sgRNA sequences for IL-1β gene transcriptional activation are ...
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bioRxiv - Bioengineering 2023Quote: ... To this mixture we added glycerol 2-phosphate (3 M, Thermo Fisher Scientific) and (GT)15-SWCNTs (3.75 mg/L ...
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bioRxiv - Cell Biology 2023Quote: ... DNA and lipofectamine (1:3 w/v) were diluted in Opti-MEM (Gibco) and incubated at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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bioRxiv - Physiology 2024Quote: ... qPCR reactions were performed in a QuantStudio 3 system (Applied Biosystems, ThermoFisher Scientific) using PowerUp™ SYBR™ Green Master Mix (cat# A25778 ...
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bioRxiv - Neuroscience 2024Quote: ... DiI crystal (1,1’-dioctadecyl-3,3,3’,3’-tetramethylin-docarbocyanine-perchlorate; D-282, Molecular Probes) was applied to the ventricular zone or pial surface ...
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bioRxiv - Neuroscience 2024Quote: Live cell cultures were incubated with TO-PRO-3 (Invitrogen; Cat. No. T3605) and Hoechst 33342 (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 3 times in PBS (pH 7.3-7.5, #28374, Thermo Fisher). Cells were incubated in DMEM (300 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked samples were analysed on a 3-8% NuPAGE Tris-acetate gel (Invitrogen), and gel bands corresponding to the crosslinked MORC2 were excised and digested as previously described above34.
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bioRxiv - Bioengineering 2024Quote: ... without or with 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) (Thermo Fisher Scientific) was added together with TG-18 to reach a final concentration of 10-20 mg/mL L-006235 (1-2% w/v ...
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bioRxiv - Microbiology 2024Quote: ... The 3 kDa tetramethylrhodamine-labeled dextran was purchased from Invitrogen (D3307, Carlsbad, CA) and was dissolved in dH2O to a final concentration of 20 mg/ml.
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bioRxiv - Immunology 2024Quote: ... Cells were passaged every 3-4 days using 0.05% Trypsin-EDTA solution (Gibco).
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bioRxiv - Molecular Biology 2024Quote: ... RNA was prepared and 3 μg was subjected to click chemistry (ThermoFisher, C10365) according to the manufacturer’s instructions ...