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Citations for Thermo Fisher :
9351 - 9400 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and analyzed with the Quant Studio 3 Real-Time PCR system (Applied Biosystems) using Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... on the 7300 or Quant Studio 3 Real-Time PCR systems (Applied Biosystems) using standard annealing and elongation protocols ...
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bioRxiv - Plant Biology 2024Quote: ... 2-3 µg of total RNA were treated with DNase I (Thermo fisher), and half was reverse transcribed using the SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Definitive endoderm was induced by 3-day exposure to RPMI 1640 (ThermoFisher #11875093) supplemented with L-glutamine (2 mM ...
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bioRxiv - Immunology 2024Quote: ... and being conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems). Detailed information on the primer and probe sets used in this study can be found in Supplemental Table 1.
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bioRxiv - Cell Biology 2024Quote: ... and counted using a Countess™ 3 Automated Cell Counter (Thermo Fisher Scientific). Cells used for assessment of Gi/o protein involvement in CCR7-stimulated migration ...
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bioRxiv - Cell Biology 2024Quote: ... 200 nM MCPH1-5’-CUCUCUGUGUGAAGCACCUdTdT-3’) in serum-Free Opti-MEM medium (Gibco). The transfection controls were set up as above but without adding the siRNA oligos ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed 3 times with hepatocyte wash medium (Thermo Fisher Scientific, 17704024). Cells were then plated in a 6-well plate precoated with collagen I (50µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Samples were amplified on the QuantStudio 3 Real-Time PCR System (ThermoFisher #A28567). Quality control and visualization were performed using the associated QuantStudio™ Design & Analysis Software ...
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bioRxiv - Genomics 2024Quote: ... 3) We did the optional wash with Phosphate Buffered Saline (PBS; Invitrogen, AM9625) containing 0.01% BSA (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... the cell media were replaced with 3 ml of FluoroBrite DMEM (#A1896702, Gibco) supplemented with 10% FBS and 10 mM HEPES (pH 7.5) ...
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bioRxiv - Physiology 2024Quote: ... qPCR reactions were performed in a QuantStudio 3 system (Applied Biosystems, ThermoFisher Scientific) using PowerUp™ SYBR™ Green Master Mix (cat# A25778 ...
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bioRxiv - Microbiology 2024Quote: qPCR was performed in the QuantStudio 3 Real-Time PCR System (Applied Biosystems) using the PowerUp SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 3 cycles of sonication in a water bath sonicator (Fisher Scientific) with each cycle consisting of 30 s of sonication followed by 2 min in an ice bath.
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bioRxiv - Neuroscience 2024Quote: ... 3 nights) and used tyramide signal amplification (Tyramide Super Boost kit from Invitrogen). After blocking non-specific binding with 10% goat serum and then any endogenous peroxidase activity with 3% H202 ...
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bioRxiv - Cell Biology 2024Quote: ... 3 μg total DNA was added to 100 μl Opti-MEM (Gibco, 31985047) and mixed ...
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bioRxiv - Neuroscience 2024Quote: ... fresh culture medium containing 33μg/mL 3 kDa dextran-AlexaFluor 488 (Invitrogen; D34682), 33 μg/mL 10 kDa dextran-AlexaFluor 647 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... was used to carry out the qPCR in a QuantStudio 3 (Applied Biosystems). Reaction was setup as follows 10 μL of TaqMan fast advanced MM ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was carried out on a QuantStudio 3 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio 3 Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). Thermocycling conditions were 50°C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The cell pellet was resuspended in 3 ml of ACK lysis buffer (Gibco) to lyse the RBCs ...
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bioRxiv - Immunology 2024Quote: Cells were loaded with 2 µg/ml Fluo-3 AM (Thermo Fisher Scientific) and 5 µg/ml Fura Red AM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 5’-TATTGGTCTCAGGGAGCGAAAGCAGG 3’ using Superscript III according to the manufacturer’s protocol (Invitrogen, #18080400).68 PCRs were performed to amplify and append partial Illumina i5 and i7 adapter sequences across four sub-amplifications of each library to sequence the entire vRNA genome ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were loaded with 3 µM Fluo-4 AM (ThermoFisher Scientific cat# F14201) diluted in basal media for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were rinsed 3 times in PBS and mounted with Fluoromount (Invitrogen™). For human cingulate gyrus stainings ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific). When noted ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated in 3-12% Bis-Tris Native gel (Invitrogen, cat. BN1001BOX) and then transferred to PVDF membrane ...
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bioRxiv - Cancer Biology 2024Quote: ... Following fixative treatment then staining with the Hema 3 kit (Thermo Fisher Scientific), quantity of cells that invaded through the VitroGel and across the Boyden chamber membrane was quantified ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then treated with 3 µl Turbo DNAse (ThermoFisher, Waltham, MA, USA) in a 50 µl reaction volume ...
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bioRxiv - Neuroscience 2024Quote: ... a Cy-3 conjugated donkey anti-guinea pig antibody was used (Molecular Probes). Fab-specific secondary antibodies were used for detection of VEGFR1 ...
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bioRxiv - Neuroscience 2024Quote: ... The final products were visualized via gel electrophoresis using 2-3% Agarose (Invitrogen) gels ...
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bioRxiv - Neuroscience 2024Quote: ... A single drop of CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher R37111) was added to individual dishes and incubated at at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... Subsequently myotubes were washed 3 times with pre-warmed DPBS (A12856-01, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... which was then transferred to 2-3 mL TRIzol Reagent (Invitrogen, Waltham, MA) to form a slurry and finally thawed ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were performed on a QuantStudio 3 real-time PCR system (Thermo Fisher) with the amplification conditions as previously described (Wilke et al. ...
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bioRxiv - Immunology 2024Quote: Bulk thymocytes or splenocytes were treated with 3 μg/ml brefeldin A (Invitrogen), without or with Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Cancer Biology 2024Quote: ... and loaded onto 3-8% gradient NuPAGE Novex Tris-acetate gels (Life Technologies). Electrophoresis was performed at 150 V for 4 h at 4°C in native gel running buffer ...
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The integrated stress response promotes macrophage inflammation and migration in autoimmune diabetesbioRxiv - Immunology 2024Quote: ... The embryos were immunostained with TO-PRO-3 (Thermo Fisher; T3605; 1:1000) to identify the nuclei and images were captured using an A1 confocal microscope (Nikon) ...
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bioRxiv - Developmental Biology 2024Quote: ... containing appropriate 3’UTRs and 30nM microRNA mimic in 96-well plates (Invitrogen, miR-92b-3p #4464066 ...
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bioRxiv - Immunology 2024Quote: ... Quantification was run on the QuantStudio 3 Real-Time PCR System (Thermo Scientific). A no reverse transcriptase (no-RT ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR was carried out on a QuantStudio 3 Real-Time PCR System (ThermoFisher) with a passive reference of ROX using the following cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: ... All experiments were performed on a QuantStudio 3 Real-Time PCR (ThermoFisher Scientific) and the raw fluorescence data was fit using a Boltzmann function to calculate the melting temperature (Tm ...
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bioRxiv - Biochemistry 2024Quote: ... as was 7- Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen™ D346) and CPM stock was prepared at 5 mg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Gene expression analysis was performed using QuantStudio 3 real-time PCR system (ThermoFisher).
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bioRxiv - Biophysics 2024Quote: ... and then cells are counted using a Countess 3 Automated Cell Counter (ThermoFisher). A cell suspension with a concentration of 15,000 cells/mL is made and 4 mL of the cell suspension is added to each well containing a coated CYTOO chip ...
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bioRxiv - Cell Biology 2024Quote: ... 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (Marina Blue DHPE) was purchased from Invitrogen. Each lipid was dissolved in chloroform within a round-bottom glass tube at a concentration of 20 mM ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were fixed for 10 mins using 3% PFA (Thermo Scientific, Cat: 28906) followed by 3X 5 mins washes with 1mg/ml NaBH4 in PBS to quench free aldehyde groups followed by three washes with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from cells 3 days after transfection using TRIzol (Thermo Fisher), and qPCR was performed to confirm EIF4EBP1 gene knockdown using TaqMan Assays (Thermo Fisher).
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bioRxiv - Cell Biology 2024Quote: ... Transduced cells were selected with 3 µg/mL puromycin (Thermo Fisher Scientific #A1113803) for K562 cells and 2 µg/mL for BHK-21 cells beginning two days after infection ...
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bioRxiv - Bioengineering 2024Quote: ... Hypersil GOLD™ C18 column (4.6 ∼ 150 mm, 3 μm, Thermo Scientific, USA) with a temperature of 30 °C ...