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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... qPCR was performed on the QuantStudio 3 Real-Time PCR System (Applied Biosystems) using SYBR Green ...
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bioRxiv - Immunology 2021Quote: ... on the QuantStudio 5 or QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific). Relative transcript levels were normalized to TATA-binding protein (Tbp ...
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Genetics 2020Quote: ... to add 3’A overhangs prior to TOPO TA-cloning (K4600-01, Invitrogen). We then performed colony PCR using the same PCR primers and conditions used to amplify the hmx1 sequence from cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s instructions and run on a Quant Studio 3 System (ThermoFisher) using the default settings for SYBR Green reagents and the fast run mode in the QuantStudio Design & Analysis Software v.1.4.2 ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were performed on a QuantStudio 3 Real-Time PCR System (Thermo Fisher) with the amplification conditions as previously described (14).
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... These lines were routinely passaged every 3-4 days using Versene (ThermoFisher; 15040066). All lines were routinely screened for differentiation and tested for mycoplasma contamination.
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bioRxiv - Neuroscience 2020Quote: ... and 0.175 g/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Invitrogen). Alkaline phosphatase staining reaction was proceeded o/n at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Gels were stained for 3 minutes with SYBR Gold (Thermo Fisher Scientific S11494), and the region corresponding to the 18-50 nucleotide sized fragments excised ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was done on QuantStudio 3 Real Time PCR Systems (Thermo Fisher Scientific) with the TaqMan gene expression Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... or a positive control probe 5’-5Alexa488N/(ATA)8TUU (ATA)7-3’ (Invitrogen). Reactions were incubated in a water bath at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2022Quote: ... JEG-3 (ATCC) were cultured in EMEM (ATCC) supplemented with 10% FBS (Gibco), penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell counts were performed 3-days after seeding using Trypan Blue (Invitrogen T10282) on Countess slides (Invitrogen C10283 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with siRNA (Table 3) using Lipofectamine RNAiMax (Life Technologies, 13778075) according to the manufacturer’s protocol for transfecting short oligonucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 (high) or 3 (vhigh) ul of RNase I (EN0601, Thermo Fisher Scientific) per 30 ug of RNA were used for digestion ...
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bioRxiv - Molecular Biology 2020Quote: Cells were transfected with plasmid DNA (Supplementary Table 3) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Ca2+ levels were determined by the Ca2+ indicator Fura 3 AM (Thermo Scientific) using confocal microscopy imaging as described previously ...
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bioRxiv - Biophysics 2022Quote: ... DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate salt) was purchased from ThermoFisher scientific (Molecular probes ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Amplification was monitored using the Quantstudio 3 Real time cycler (Thermo Fisher Scientific). ß-globin (primers forward ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl of Lipofectamine 2000 in 47 μl of OptiMEM (ThermoFisher Scientific) by pipetting up and down ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24 hrs at 1 hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Immunology 2020Quote: ... Apoptosis was detected using the CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher). Frames were captured over a period of 24hrs at 1-hour intervals from 4 separate 1.75 x 1.29 mm2 regions per well with a 10× objective using IncuCyte S3 live-cell analysis system (Sartorius) ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded dropwise overnight onto a 3 ml Ni-NTA agarose (ThermoFisher Scientific) column (Qiagen 30230 ...
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bioRxiv - Genetics 2021Quote: ... to detect HSA21 and biotin-labeled I-EGFP-I-loxP-3’HPRT (ThermoFisher) to detect EGFP on the HSA21 ...
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bioRxiv - Cancer Biology 2020Quote: qPCR analysis was performed on a QuantStudio 3 Real-Time instrument (ThermoFisher Scientific) utilizing the Power SYBR™ Green PCR Master mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... using qPCR (QuantStudio 3 cycler, Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Genomics 2021Quote: ... on a QuantStudio 3 cycler (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA). The reaction was conducted in triplicates ...
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bioRxiv - Genomics 2020Quote: ... 3% of DMSO and 1X Phusion Master Mix (ThermoFisher Scientific, Waltham, MA, USA). PCR amplifications (98 °C for 2 min ...
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bioRxiv - Immunology 2021Quote: JEG-3 cells were infected with ZIKV for 1 h in DMEM (Gibco) with 2% FCS at an MOI of 1 ...
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bioRxiv - Immunology 2021Quote: ... In vitro derived PCs were stained with CellEvent Caspase 3/7-Green (ThermoFisher), tetrametylrhodamine ...
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bioRxiv - Neuroscience 2020Quote: ... Cell nuclei were detected with TO-PRO-3 (1:400; Thermo Fisher Scientific). The retinal and optic nerve slices were analyzed with a confocal laser-scanning microscope (LSM 510 META ...
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bioRxiv - Bioengineering 2021Quote: ... 96-well PCR plates were read using a Quantstudio 3 System (ThermoFisher Scientific) and results were analyzed using the delta-delta CT method ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell counts were performed 3-days after seeding using Trypan Blue (Invitrogen T10282) on Countess slides (Invitrogen C10283 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed 3 times with PBS and counterstained with DAPI (Invitrogen) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and blocking solution (PBS with 0.1% triton, 3% normal donkey serum; Fisher Scientific). The sections were incubated overnight at room temperature in rabbit anti-FOS (FOS ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody incubation was performed for 3-4 days and DiD (Thermo Fisher Scientific-Molecular Probes L7781 ...
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bioRxiv - Neuroscience 2020Quote: ... At DIV 3 the medium was changed to Neurobasal medium (Thermo Fisher Scientific) supplemented with Forskolin (10 μM ...
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bioRxiv - Cell Biology 2021Quote: ... JEG-3 cells (ATCC-HTB-36) were grown in MEM (Gibco, #51200-046), 10% FCS ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: 3 × 104 cells were transfected BRE-Luciferase reporter plasmid using Lipofectamine 2000 (Invitrogen). The cell lysates collected 48 hr after transfection ...
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bioRxiv - Microbiology 2020Quote: ... MH96 was grown overnight (18 h) in 3 ml Luria Broth Base (Invitrogen) with shaking (250 rpm ...
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bioRxiv - Bioengineering 2021Quote: ... were all purchased from Sigma and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium was purchased from Thermofisher.
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bioRxiv - Bioengineering 2020Quote: ... T cells were cultured for 3 days with CD3/CD28 Dynabeads (ThermoFisher Scientific) with 40 IU/mL IL-2 and spinoculated with lentivirus for 2 hours at 400 rcf in the presence of 8 µg/mL polybrene ...
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bioRxiv - Biochemistry 2020Quote: ... and cells were loaded with 3 μM Fura-2 AM dye (Molecular Probes) in sterile-filtered HEPES-Tyrode Buffer (HTB ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae (6 dpf) were transferred to a 3 cm Petri dish (Thermo Scientific) and allowed to acclimatize for 5 min before video recording ...
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bioRxiv - Neuroscience 2021Quote: ... the culture medium was replaced 3 h after plating by Opti-MEM (Invitrogen) before the DNA mixture was added ...