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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Neuro2a and HEK293T cells were obtained from ATCC and maintained in Dulbecco’s modified Eagles medium supplemented with 5% fetal bovine serum and Penicillin/Streptomycin (Gibco). 3 x 105 cells were seeded in 6-well plate and maintained by indicated times in DMEM cell culture media supplemented with 5% bovine fetal serum and non-essential amino acids ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Mouse midbrains (n = 5-6 per group per age) were collected and RNA was isolated using TRIzol Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: OMVs were boiled at 95°C for 5 minutes with lithium dodecyl sulfate (LDS) sample buffer (4X Bolt; Invitrogen) and run on a NuPAGE 12% Bis-Tris gel (Invitrogen ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then further fixed with 1.5 mM BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate) (Thermo Scientific A35396) in 1× PBS at room temperature for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Ganglia were dissociated by trituration using a fire polished Pasteur pipette, followed by centrifugation (5 minutes, 160 x g) and trituration in wash buffer (DMEM Ham’s F-12, Gibco, 10565-018 ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Genomics 2021Quote: ... Samples were transferred to 1.5 mL safe-lock tubes containing one scoop of acid-washed glass beads (<106μM) and 1 mL TRIzol (Invitrogen) containing 20μg/mL GenElute-LPA (Sigma-Aldricht ...
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bioRxiv - Genetics 2021Quote: ... urartu was labelled by nick translation with ChromaTide™ Alexa Fluor™ 488-5-dUTP (Invitrogen; C11397; coloured green), (2 ...
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bioRxiv - Neuroscience 2020Quote: ... All neurons were maintained at 37°C and 5 % CO2 in glia- and cortex-conditioned Neurobasal-A (Life Technologies) supplemented with B27 and GlutaMax ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were washed and preconcentrated on PepMap™ C18 precolumns (5 mm x 300 μm inner diameter; Thermo Scientific) and separated using AcclaimPepMap™ RSLC columns (50 cm x 75 μm inner diameter ...
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bioRxiv - Microbiology 2020Quote: The Vero cells were maintained at 37℃ under 5% CO2 in Dulbecco’s modified Eagle essential medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco), 10 mM HEPES and 1% penicillin/streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 5 mg of cecum tissue was removed and placed in RNAlater and stored at −80°C (Thermo Fisher). The remaining cecum (approximately two thirds ...
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bioRxiv - Neuroscience 2020Quote: ... Cell-attached recordings were performed with aCSF + 5 μM Alexa Fluor® 488 nm (Molecular Probes Cat# A-10440) or 594 nm (Molecular Probes Cat# A-10442 ...
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bioRxiv - Neuroscience 2020Quote: ... Eyes were removed quickly using Dumont’s forceps #5 and put in ice-cold Hibernate A medium without calcium and magnesium (Invitrogen). Under a dissection scope ...
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bioRxiv - Pathology 2021Quote: ... were grown at 37°C in a humidified 95% air/5% CO2 incubator in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% v/v heat-inactivated fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilized using PBS with 0.2% Triton X-100 for 15 min and blocked with 5% FBS (Gibco), followed by treatment with the primary antibody anti-SARS-CoV-2 NP (GeneTex GTX635678 ...
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bioRxiv - Systems Biology 2020Quote: ... Primary fibroblasts were expanded and cultured at 37°C in 5% CO2 in fibroblast medium (Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco) with 100 mg/mL penicillin/streptomycin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... and African green monkey kidney epithelical BS-C-1 cells (ATCC, CCL-26) were maintained at 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... for 1hour and labelled with 1μM CFSE (CFSElow) or 5 μM CFSE(CFSEhigh) respectively following manufacturer’s instructions (Life Technologies). A mixture of 2×106 CFSElow and CFSEhigh splenocytes were intravenously injected to gender matched naïve mice or the mice which had been intranasally infected 8 days ago with H1N1 or H5N1(2:6 ...
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bioRxiv - Microbiology 2020Quote: ... The 10 μl reactions contained 5 μl of SYBR Green ROX qPCR (Thermo Fisher Scientific Inc., Wilmington, DE, USA), 0.2 μl of bovine serum albumin (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Freshly dissected tissues were incubated for 5 min in 50 nM LysoTracker Red DND-99 (Life Technologies, Cat# L7528) in PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... The siRNAs at a concentration of 5 nM were reverse transfected using Lipofectamine RNAiMAX transfection reagent (Invitrogen, VIC, Australia) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells described above were cultured at 37C at 5% CO2 in Advanced Dulbecco’s Modified Eagle Medium: Nutrient mixture F-12 (DMEM-F12) with Glutamax (GIBCO), supplemented with 12% FCS (Clontech) ...
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bioRxiv - Cancer Biology 2020Quote: ... Ming-Chih Lai) (50 ng) or mutant MT-STAT1-5’UTR-Luciferase (50 ng) using Lipofectamine 3000 (Life Technologies). In HepAD38 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... India were maintained in a CO2 incubator at 37°C and 5% CO2 concentration in high glucose Dulbecco’s modified Eagle medium (DMEM) with L– glutamine (Gibco) supplemented with 10% fetal bovine Calf serum and 100 U/ml penicillin-streptomycin (Himedia).
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bioRxiv - Cancer Biology 2021Quote: For this analysis 1/5 of the total EV pellet per sample was resuspended in 10 μL PBS (Gibco) and stored at 4°C for up to 7 days ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were starved for 72 hours in phenol-red free media supplemented with 5% charcoal stripped serum (CSS) (Gibco) followed by stimulation with 10nM R1881 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: A reverse transcription (RT) reaction was performed in 50 μL containing 5 μL of 10× SuperScript III buffer (Invitrogen), 5 μL of 50 mM MgCl2 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with 5 μM CellROX Green staining solution for 30 mins per manufacturer’s instructions (Invitrogen, cat# C10492). Six-seven different visual fields were acquired with Leica SPE confocal microscope and Mean Fluorescence Intensity (MFI ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... and lifted for 3 min at 37°C using 5 mL of TrypLE Express (Invitrogen, Gibco, Cat no. #1260501). The appropriate number of cells were then spun down at 90 x g for 10 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 5 mM DTT) and then mixed with 50 mM (final 6 mM) disuccinimidyl dibutyric urea (DSBU) (Thermo Fisher Scientific). Crosslinking reaction was incubated on ice for 2 hours and then was quenched by adding 1M (final 50 mM ...
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bioRxiv - Biophysics 2021Quote: ... passage 3-14) were cultured at 37 °C with humidified air containing 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: Time plug-mated Pbx3−/- mice received a single intraperitoneal injection of 5-ethynyl-2’-deoxyuridine (EdU; 0,1mg/g animal; Invitrogen) at E15.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 5 min with 2 μM LysoSensor Yellow/Blue DND-160 (L7545, Invitrogen/Thermo Fisher Scientific) in extracellular Ringer’s solution at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... the slides were counterstained with DAPI for 5 min and mounted with ProLong™ Gold Antifade Mountant (Thermofisher Scientific). Slides were scanned for image acquisition using Zeiss AxioScan Z.1 and ZEN2 software.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA samples were diluted 5-fold and it was performed with StepOne Plus Real Time PCR System (Applied Biosystems). The FastStart Universal SYBR Green Master Mix (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in flow buffer plus 5 μL of propidium iodide (PI) staining solution (10 μg mL-1, Invitrogen) per sample ...
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bioRxiv - Developmental Biology 2022Quote: ... After multiple 5-min washes in PBS and 90-min incubation with AlexaFluor-conjugated secondary Ab as above (Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... the samples were bound to a precolumn (Acclaim C18 PepMap100, 300 μm i.d., 5 mm length; Thermo Fisher Scientific) and then eluted onto the analytical column (SilicaTip Emitter ...
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bioRxiv - Neuroscience 2022Quote: ... Cortical astrocytes were plated at a density of 2.5 × 104 to 5 × 104 cells.cm−2 in Minimum Essential Media (Gibco, MEM) supplemented with 10% Horse Serum (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... The qPCR reactions were performed in triplicate using a QuantStudio 5 Real-time PCR System (Thermo Fisher, cat#A34322) using the following thermocycling conditions ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in sorting buffer (0.2% BSA in PBS) containing 5 nM SYTOX Green Nucleic Acid Stain (catalog number S7020; ThermoFisher) to distinguish between live and dead cells ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells were grown in LB media (Difco LB Broth, Miller [Luria Bertani,] Fisher Scientific, Catalog #DF0446-07-5) for reporter gene and RT-qPCR assays ...
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bioRxiv - Cell Biology 2022Quote: ... Northwestern University) cells were cultured at 37 °C with 5 % CO2 in Dulbecco’s modified eagle’s medium (DMEM, Life Technologies) containing 10 % fetal bovine serum (Seradigm ...
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bioRxiv - Cell Biology 2022Quote: ... After incubation cells were incubated for 5 min at RT with TMR-phalloidin (0.83 μg/mL) and Hoechst 33342 (1 μg/mL, Invitrogen) in PBS and washed with blocking buffer before imaging on a Zeiss Axiovert 200 equipped with an Andor Luca camera.
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bioRxiv - Biochemistry 2022Quote: ... 2 µL of 20 µM siRNA and 5 µL RNAiMAX were diluted in 400 µL OptiMEM (Thermo Fisher Scientific), mixed gently and incubated for 20 min at room temperature ...