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Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: Fc fusion proteins were expressed in ExpiCHO cells using the ExpiCHO expression system kit (Thermo Scientific, A29133) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... N-glycosylation modification of proteins was assessed using the PNGase F Glycan Cleavage Kit (A39245, Gibco, USA). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... We detected the protein signal using the Enhanced Chemiluminescence Kit (32106, Thermo Fisher Scientific, Waltham, MA, USA) with the FUSION FX imaging system (Vilber Lourmat ...
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bioRxiv - Molecular Biology 2022Quote: ... AHA incorporated in proteins was detected using dedicated Click-iT Cell Reaction Buffer Kit (Thermo Fisher Scientific) and Alexa Fluor 594 Alkyne (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... cytosol and membrane fractions were isolated using the Mem-Per Plus Membrane Protein Extraction Kit (89842, Thermofisher) following product guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Quantities of cortical proteins were determined by Pierce™ Detergent Compatible Bradford Assay Kit (Thermo Scientific™).
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bioRxiv - Developmental Biology 2023Quote: ... The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher, Q33211). The protein products were diluted to 1 µg/mL in L-15 and stored at -80 °C until use.
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bioRxiv - Microbiology 2023Quote: ... with 10 µl of each sample and 200 µl of Coomassie (Bradford) Protein Assay Kit (Thermo Scientific) and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent tagging of mBSA was performed using Alexa Fluor 594 Microscale Protein Labelling kit (Invitrogen, Molecular Probes) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent tagging of mBSA was performed using Alexa Fluor 594 Microscale Protein Labelling kit (Invitrogen, Molecular Probes) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Protein quantification was done using the bicinchoninic acid (BCA) rapid gold assay kit (Pierce, Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... sterile-filtered protein solution using the Pierce LAL Chromogenic Endotoxin Quantitation Kit (Thermo Fisher Scientific, Dreieich, Germany) essentially following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... and then total protein concentration in the supernatant was determined by BCA assay kit (Thermo Fisher Scientific). Proteins in the supernatant were separated on a 10% polyacrylamide gel by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and then total protein concentration in the supernatant was determined by BCA assay kit (Thermo Fisher Scientific). Proteins in the supernatant were separated on a 10% polyacrylamide gel by SDS-PAGE ...
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bioRxiv - Bioengineering 2023Quote: miRNA was isolated from MBVs using the Total Exosome RNA and Protein Isolation Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cytoplasmic and nuclear proteins were extracted using NE-PER nuclear and cytoplasmic extraction kit (Thermo Scientific, #78833). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA pull down was performed using the Pierce Magnetic RNA-Protein Pull-Down Kit (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were detected using the SuperSignal West Pico PLUS Chemiluminescent Substrate kit (#34580, ThermoFisher Scientific, MA, USA) and images were taken with a Uvitec Cambridge (Uvitec Cambridge ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA pull-down was performed using a Magnetic RNA-protein Pull-Down Kit (Thermo Fisher Scientific, 20164) with the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... We measured total protein concentration of each sample using the Pierce Detergent Compatible Bradford Assay Kit (ThermoFisher). Aβ38 ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of histones in each sample was determined using the BCA protein assay kit (ThermoFisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: Cytosolic and chromatin fractions were quantified by Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific #23235) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Subcellular fractionation was performed using the Subcellular Protein Fractionation Kit for Cultured Cells (Cat# 78840, Thermo Scientific) following the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein signals were detected using the SuperSignal West Femto Chemiluminescent kit (Thermo Fisher Scientific, Waltham, MA, USA) and a LAS-3000 imaging system (Fujifilm ...
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bioRxiv - Cell Biology 2023Quote: ... a cell-surface biotinylation assay was performed using Pierce Cell Surface Protein Isolation Kit (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... and the antibodies were purified using NAb protein A plus spin kit (Thermo Fisher Scientific, Cat# 89948) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... a fraction of the supernatant was subjected to a Bradford assay (Bradford Protein Assay Kit, 23200, ThermoFisher). The rest of the supernatant was mixed with 4x SDS sample buffer (250 mM Tris-HCl at pH 6.8 ...
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bioRxiv - Cell Biology 2024Quote: ... conjugated with Alexa Fluor 555 using Alexa Fluor 555 Microscale Protein Labeling Kit (Thermo Fisher Scientific, A30007) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... GST used as a control was labeled with Alexa Fluor 594 Microscale Protein Labeling Kit (A30008, Invitrogen) following the manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2024Quote: ... The EMSA was performed to assess the protein-DNA interactions with an EMSA kit (Thermo Fisher Scientific), following the manufacturer’s guidelines to ensure standardization and reproducibility of the assay.
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bioRxiv - Biophysics 2024Quote: ... A colorimetric kit was used measure the reduction of Cu2+ to Cu1+ by protein (Thermo Fisher Scientific). The color change was determined by measuring the optical density at a wavelength of 562 nm using a SpectraMax platereader (Danaher Life Sciences ...
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bioRxiv - Immunology 2024Quote: ... Total protein concentration was measured using a standard Bradford assay (Pierce Detergent Compatible Bradford Assay Kit, ThermoFisher), and 150ugs of total protein was incubated on membranes pre-spotted with capture antibodies for 59 immune-related receptors ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C) and the protein concentration determined by a BCA assay (Pierce BCA Kit, Thermo Fisher Scientific). The human protein extracts were further processed to peptides in the same manner as described above for bacterial protein extracts.
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bioRxiv - Bioengineering 2022Quote: ... Fluorophore-conjugated secondary antibody staining (anti-rabbit AlexaFluor® 488, anti-mouse AlexaFluor® 594, anti-mouse AlexaFluor® 647; Molecular Probes; 1:800) was performed at room temperature for 1 h in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were treated with goat-anti-mouse IgG Alexa Fluor 488 and goat-anti-mouse IgG Alexa Fluor 555 for 1 hour (both from Thermo Fisher Scientific, MA, USA). The staining procedure was conducted using the Antifade Reagent with DAPI (Cell Signaling Technology ...
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bioRxiv - Immunology 2023Quote: ... A secondary antibody was used in TBS-T with a dilution of 1:5,000 for Anti-Mouse (Thermo Fisher, Goat anti-Mouse IgG, #31430) and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG ...
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bioRxiv - Neuroscience 2023Quote: ... Iowa City, IA), mouse anti-Futsch (22C10 DSHB, Iowa City, IA) and mouse anti-GFP at 1:1000 (#A-11120, Thermo Fisher Scientific, Waltham, MA). Secondary antibodies used in this study were goat anti-mouse Alexa Fluor 488-conjugated IgG at 1:500 (#A-32723 ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein detection was done using a custom antibody against MIPS (1:10,000) and anti-actin (1:10,000; Invitrogen MA1-744). The custom MIPS antibodies were produced by YenZym Antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The surviving clones were screened for expression of the desired proteins in the presence of 1 μg ml−1 doxycycline (Invitrogen). Expression of Myc-Cdk11 was also induced with 1 μg ml−1 doxycycline in the subsequent experiments.
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bioRxiv - Biochemistry 2020Quote: ... 1 mM TCEP and SYPRO orange protein gel stain at a 1:500 dilution of the manufacturer’s stock (Invitrogen, S6651). The samples also contained 5 μM NBD protein ...
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bioRxiv - Pathology 2020Quote: ... 1 mM EDTA and 1% SDS) and the protein concentration was quantified by the BCA assay (Thermo Fisher Scientific, #23225). Equal amount of whole cell extracts were resolved by SDS-PAGE and transferred to PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2024Quote: ... Any bound proteins were eluted by adding a 1:1 mix of 10 mM TBS and LDS buffer (Life Technologies) to the first well and then transferring from well to well ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... chromatin-antibodies complexes were recovered by incubation with 30 μl of Dynabeads® Protein A/G 1:1 mix (ThermoFisher) for 2 h at 4°C under rotation ...
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bioRxiv - Genomics 2023Quote: ... Sonicated samples were pre-cleared to remove background signal through incubation with a 1:1 mix of Protein A/G Dynabeads (InVitrogen). Antibody was added at a concentration of 1 µg/µL per sample and immunoprecipitated at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed before developed using either the SuperSignal ELISA Femto Substrate (Thermo Fisher Scientific) or ELC Prime Western Blotting system (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Cell culture supernatant was harvested and used to carry out ELISA for IL-1β (Invitrogen) and IL-18 (Invitrogen ...