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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Microbiology 2020Quote: ... were calculated by IFA using primary antibodies against viral proteins and Alexa FluorTM 488 α-rabbit and Alexa FluorTM 586 α-mouse as secondary antibodies (Invitrogen), respectively.
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to create KPCN cells expressing mouse NSDHL protein we used lentiviral construct pLEX-HA-MYC (#OHS4492, Thermo Scientific Open Biosystems). Lentivirus production was performed by co-transfecting HEK293T packaging cells with mNSDHL coding transfer vector and packaging plasmid mix included in Trans-Lentiviral ORF packaging kit with Calcium Phosphate (#TLP5916 ...
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bioRxiv - Pathology 2021Quote: ... lysis of cells and mouse liver tissues was achieved using the Mammalian Protein Extraction Reagent (cat 78501; Thermo Fisher Scientific, Waltham, MA) in association with the Halt Protease Inhibitor Cocktail (cat 78429 ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...
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bioRxiv - Neuroscience 2022Quote: ... and then with buffer containing 250 nM purified His-tagged protein that was previously pre-clustered with anti-His (mouse; Thermo Fisher Scientific) in a 2:1 (protein:antibody ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was immunoprecipitated using 2 μg/ml anti-mouse ST2 antibody and Pierce™ Protein G Magnetic Beads (ThermoFisher, Cat# 88848), followed by western blot analysis.
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (rabbit IgG-, Protein A-, or mouse IgG-conjugated with HRP, Thermo Fisher Scientific) and ECL detection reagent (Amersham) ...
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bioRxiv - Developmental Biology 2024Quote: ... ECs isolated from each mouse were seeded in 2 wells of 6-well plate coated with attachment factor protein 1X (ThermoFisher Scientific, S006100). For a pure EC population ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva).
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bioRxiv - Cell Biology 2023Quote: ... The blot was washed three times in .05% PBST and HRP-conjugated goat anti-mouse secondary was added at 1:2000 for 1 hour (Invitrogen, 31430, 1:2000). U2OS cell western blot quantification was carried out using the ImageQuant™ software (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... The rabbit anti-10-mer serum was further purified using Protein A IgG purification kit and conjugated to agarose beads by AminoLinkTM Immobilization kit (Thermo Scientific TM Pierce TM). Other rabbit polyclonal antibodies included anti-ELKS and anti-IKKε antibodies (clone D20G4 ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Developmental Biology 2021Quote: ... 35 μm thick sections were used immunohistochemistry (IHC) of acetylcholine receptors (α-Bungarotoxin extracted from Bungarus muticinctus, conjugated to Alexa Fluor 647, Thermofisher #B35450) and nerve fibers (chicken anti-neurofilament-H antibody ...
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bioRxiv - Genomics 2020Quote: Plasmid p3xFLAG-CMV-14 carrying the complete sequence for progesterone receptor gene (HindIII924 - 938EcoRI) was introduced in Ishikawa cells using Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer recommendations ...
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bioRxiv - Microbiology 2020Quote: ... SVECs lacking the IFN-α/β receptor (IFNAR1-CRISPR) were maintained in the same media as SVECs with 2 μg/mL puromycin (Gibco). Primary LECs (Cell Biologics ...
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bioRxiv - Microbiology 2021Quote: ... Vero-hSLAMF1 cells [52] stably express the human measles receptor SLAMF1 and were cultured in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific) containing 5% newborn calf serum (NCS ...
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bioRxiv - Cell Biology 2024Quote: ... The cell membrane of mESCs without HaloTag-receptors was fluorescently stained with wheat germ agglutinin Alexa Fluor 488 (Thermo Fisher Scientific, W11261) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... The cells were incubated in the diluent buffer (ab64211) of the primary polyclonal Anti-CYP11B1 antibody (ab197908) and the TSH Receptor monoclonal antibody (4C1) (Invitrogen, MA5-16519) at a dilution of 1:200 for 1 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl- β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... were collected 2 days post-infiltration and ground to a homogeneous powder in liquid nitrogen and resuspended in 1 mL of protein extraction buffer (1× PBS, 1% n-dodecyl β-d-maltoside or DDM (Invitrogen, Carlsbad, CA, USA), and 0.1 tablet cOmplete™ protease inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: Total proteins were isolated from GC cell and the concentration was measured with BCA Kit (ThermoFisher, USA). Proteins were separated by 10% SDS-PAGE gel and transferred to 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Csm complex concentration was measured according to the Bradford method using the protein assay kit (Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Molecular Biology 2021Quote: Total S-nirosylated proteins in the BAT was detected by S-nitrosylation Western Blot Kit (ThermoFisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... plasma and cell lysates was quantified using a micro BCA™ protein assay kit (23235, ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration of cell lysates was determined with the BCA assay kit (Thermo Fisher Scientific, Schwerte, Germany).
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... Imaging of the protein bands on the blot was conducted using the ECL kit (Thermo Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: RNA from porcine samples was isolated using Sure Prep RNA/DNA/Protein Purification Kit (Fisher Scientific, USA) for cells ...
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bioRxiv - Developmental Biology 2021Quote: Membrane fractionations were performed using the Mem-PERTM Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, 89842). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Bioengineering 2020Quote: ... Protein-digested raw extracellular DNA sample was finally purified using GeneJET NGS Cleanup Kit (Thermo Scientific, USA). Obtained free-floating extracellular DNA samples were stored at -20°C for further analysis.
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bioRxiv - Bioengineering 2020Quote: Immunoprecipitation of hsACE2 from BALF was conducted using Dynabeads™ Protein G Immunoprecipitation kit (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: Co-immunoprecipitation was conducted using a NAb™ Spin Kit (Protein G, 0.2 mL; Thermo Fisher Scientific). Briefly ...
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bioRxiv - Bioengineering 2021Quote: ... and protein digestions were conducted using an In-Solution Tryptic Digestion and Guanidination Kit (89895, ThermoFisher Scientific)‡ ...
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bioRxiv - Plant Biology 2021Quote: For recombinant protein expression in Pichia pastoris GS115 (Multi-Copy Pichia Expression Kit Instructions, Thermo Fisher Scientific), constructs of IF1 ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration in the cell lysate and media was measured by BCA kit (ThermoFisher, MA, USA)as per the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... pylori for 24 hours were extracted using ProteoJet™ Membrane Protein extraction kit (Fermentas, Thermo Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Equal loading was checked by staining PVDF membranes with Pierce Reversible Protein Stain Kit (Thermo Fisher Scientific), Ponceau S or Coomassie Brilliant Blue staining solutions.
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant protein concentrations were determined using a commercially available BCA kit (Thermo Fisher Scientific; Waltham, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total protein concentration in each sample was measured using Pierce® BCA Assay Kit (Thermo Fisher Inc.), and samples were mixed with post-lysis Laemmli buffer (2 % SDS ...
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bioRxiv - Cancer Biology 2020Quote: ... Supernatant was collected and quantified using the BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA). To prepare the super-SILAC standard ...
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bioRxiv - Cell Biology 2021Quote: ... cytosol and membrane fractions were isolated using the Mem-Per Plus Membrane Protein Extraction Kit (89842, Thermofisher) following product guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using a Pierce 660 nm kit with ionic detergent compatibility reagent (Thermo Fisher Scientific Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined using Pierce 660 nm kit with ionic detergent compatibility reagent (Thermo Fisher Scientific), and 12 μg of each sample was loaded on a 12.5% SDS-PAGE gel and run ~1 cm into the resolving gel ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Total islet protein in hydrolyzed samples was measured using a BCA assay kit (Thermo Scientific, Cat #: 23235) following pH neutralization with NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration in the supernatant was estimated using the Pierce Detergent Compatible Bradford Assay Kit (Thermofisher, 23246). For each Western Blot ...