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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... with the anti-HRPK-1 antibody covalently crosslinked to the Protein A Dynabeads (ThermoFisher) using dimethylpimelimidate1 ...
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bioRxiv - Immunology 2019Quote: ... 1× protein transport inhibitor cocktail (PTIC) containing Brefeldin A/Monensin (eBioscience, Thermo Fisher Scientific) with or without anti-CD107a (eBio1D4B ...
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bioRxiv - Plant Biology 2019Quote: ... The protein was expressed in sf21 insect cells using the vector pFastBac 1 (Invitrogen). One litre of cells (2.5×106 cells ml−1 ...
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bioRxiv - Microbiology 2019Quote: ... Membranes were washed and incubated with horseradish peroxidase-coupled protein G (1:1,000; Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl Dynabeads Protein A (Invitrogen 10001D) coated with 10 µg GINS antibodies for 2 h at 4°C with rotation ...
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bioRxiv - Immunology 2022Quote: ... at a protein/trypsin ratio of 1:50 and 10 % acetonitrile (Thermo Fisher Scientific), in a final volume of 100 μl ...
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bioRxiv - Pathology 2024Quote: ... The protein mixture was separated on a 4-12% NuPAGE gel (1 mm, Invitrogen). Samples were run at room temperature for 35 min at 200 V ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected in localization experiments using Streptavidin-Alexa674 (Life Technologies, 1:1200).
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bioRxiv - Genomics 2023Quote: 1 μg of antibody was coupled with 10 μL protein A (Invitrogen, cat# 10002D) and 10 μL protein G (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and Chlamydia heat-shock protein 60 (cHsp60) (ThermoFisher Scientific MA3-023, 1:2000 dilution), followed by secondary antibodies AlexaFluor 488 anti-mouse (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 diluted lysates were quantified using the Pierce BCA protein assay (Thermo Scientific). 30 μg of protein lysates were mixed with equal parts 2x Loading buffer (21% w/v glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... was used to stain proteins and SYTOX™ Deep Red (1 µM, ThermoFisher S11380) was used as a DNA stain.
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 mg of total protein was resuspended in 100 μl NativePAGE sample buffer (Invitrogen), lysed with 1.5% digitonin (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... rat anti-glial fibrillary acidic protein (GFAP) antibody (Thermofisher, Cat# 13-0300, 1:70), and chicken anti-microtubule-associated protein 2 (MAP2 ...
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bioRxiv - Cell Biology 2023Quote: ... All other proteins were detected with secondary antibodies conjugated to DyLight800 (1:10000, ThermoFisher). Images were acquired with a ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies to the following proteins were used: RhoA (Invitrogen, #PA5-87403, 1:500), Rac1 (Invitrogen ...
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bioRxiv - Physiology 2023Quote: The extracts (1 mg protein) were pre-cleared with control 4%-Agarose resin (ThermoFisher) for 1h at 4 °C with constant rotation ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and then stained using 1:300 α-mouse-AF488 (Molecular Probes A11001) in 1 % BSA for 30 min at RT in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and secondary antibodies (Alexa Fluor 488-conjugated goat anti-mouse IgG [1:1,000] and Alexa Fluor 568-conjugated donkey anti-rabbit IgG [1:1,000], Molecular Probes). Fixed neurons were incubated with primary antibodies followed by secondary antibodies and mounted on slides as described (Moon et al ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated for 1 h at room temperature with 1:500 donkey anti-mouse 647 secondary antibody (Life Technologies). After washing in PBST and PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were resuspended in PBS containing 1% BSA and 1:300 Alexa 647 conjugated donkey anti-mouse (Thermofisher, A21238) and incubated for 30 min at room temperature and in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAb anti-ZO-1 (clone ZO1-1A12) used in immunofluorescence 1:300 on zebrafish embryos was from Invitrogen. Rat mAb anti-HA High Affinity (clone 3F10) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were washed with TBST and further reacted either with alkaline phosphatase-conjugated anti-mouse or anti-rabbit IgG secondary antibody (1:2000) or with horse radish peroxidase-conjugated anti-mouse or anti-rabbit IgG antisera (1:20,000) (Invitrogen) at room temperature for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... (1/2,000) followed by anti-rabbit Alexa 488- and anti-mouse Alexa 594-conjugated IgG (1/2,000, Life technologies). Samples were observed at 100X magnification using a Leica DMi8 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed three times with PBST and incubated with the correspondent secondary antibody (diluted in 1% BSA in PBST) (goat anti-mouse or anti-rabbit conjugated to AlexaFluor 594 or 488 1:1,000 (Invitrogen)) for 1 hour at RT in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were activated using a 1:1 ratio Dynabead Mouse T Activator CD3/CD28 (#00775477, Thermo Scientific) for 72 hrs in T cell media containing RPMI 1640 (VWR) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... rabbit primary antibody of NPY (at dilution of 1:1,000) and mouse primary antibody of eNOS (at dilution of 1:2,000) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and stained for another 1 hr using 1:1,000 dilution of Alexa Fluor 568 goat anti-mouse IgG (Invitrogen) as a secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... followed by secondary antibody incubation (1 h at room temperature) with fluorescein-labeled anti-mouse IgG (1:200, Invitrogen) in 3% normal goat serum in PBS ...
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bioRxiv - Immunology 2019Quote: ... conjugated rabbit anti-mouse (1:64) or AlexaFluor 594-conjugated rabbit anti-donkey antibody (1:250; Invitrogen, Paisley, UK). All secondary antibodies had been tested and found to be negative for cross-reactivity against human epithelial cells ...
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bioRxiv - Developmental Biology 2019Quote: ... prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies, Carlsbad, CA; 1A12; 1:100). Following further buffer washes ...
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bioRxiv - Cell Biology 2020Quote: ... WB: 1:10,000) and goat anti-mouse-HRP (Cat# 31430, WB: 1:10,000) antibodies were purchased from Thermo Fisher. Alexa Fluor 488/594/647 conjugated secondary antibodies for immunofluorescence were purchased from Jasckson ImmunoResearch.
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bioRxiv - Neuroscience 2021Quote: ... but after rinsing sections were incubated in secondary antibodies (donkey anti-guinea pig 555 1:1,000 Sigma SAB4600298; goat anti-mouse 546 1:400 Invitrogen A-11030 ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 5 times with the wash buffer and then incubated at room temperature for 1h with Alexa 488-conjugated chicken anti-mouse secondary antibody together with Alexa-568-conjugated phalloidin (1:500) and Hoechst (1:10,000; Invitrogen). Cells were then washed 8 times with wash buffer and mounted using mowiol (Sigma ...
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bioRxiv - Genetics 2022Quote: ... Incubation with the primary antibody (SDC-1 (1:200, CD138 Mouse anti-Human, PE, Clone: MI15, Fisher Scientific, UK) for SynT and HLAG (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with one of the following secondary antibodies: 1) donkey anti-mouse 488 (1:250; Invitrogen, USA); 2 ...
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bioRxiv - Neuroscience 2022Quote: ... chicken anti-GFP, Abeam, Cat# ab13970; 1:200, mouse anti-GFP, Roche, Cat# AB_390913; 1:1000, rabbit anti-GFP, Invitrogen, Cat# A-l 1122 ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-mouse (488, 1:300, Thermo Fisher Scientific, Cat. No. A21022, RRID: AB_141607 or 594, 1:300, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were successively incubated with indicated primary antibodies in PBS/BSA 1% saponin 0.1% for 2 h at RT and with Alexa Fluor-conjugated secondary antibodies in PBS/BSA 1% saponin 0.1% for 1 h raised against mouse or rabbit immunoglobulins (Invitrogen). After washes ...
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bioRxiv - Biochemistry 2023Quote: ... coverslips were incubated for 1 hr at 37°C with goat anti-mouse AlexaFluor 488 (A11001; ThermoFisher; 1:500) secondary antibody diluted in blocking buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen, A21202, 1:800 in 1% BSA/1X PBS) or Alexa Fluor 647 goat anti-rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and secondary antibodies (i.e., anti-mouse, 1:3000 and anti-rabbit IgG, 1:4000 conjugated to horseradish peroxidase; Invitrogen 31432/AB_228302 and 31460/AB_228341 ...
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bioRxiv - Neuroscience 2023Quote: ... and either AlexaFluor-488/647-conjugated goat anti-mouse or anti-rabbit IgGs (1:400) and Hoechst (1:10,000; Invitrogen). For tissue expansion ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 1 µL mouse anti-V5 primary antibody (1:10,000; R96025, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 1 hr incubation in primary antibody solution at room temperature (1:1,000 mouse anti-v5; R96025, Invitrogen). Cells were washed twice with DPBS and incubated with secondary antibody (1:1,000 donkey anti-mouse 568 ...
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bioRxiv - Cancer Biology 2024Quote: ... The Macrophage panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:50, 48-3697-82, Invitrogen) and anti-mouse Ki67-PE/Cyanine7 (1:150 ...
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bioRxiv - Cancer Biology 2024Quote: ... while the MDSC panel’s intracellular primary antibodies consisted of anti-mouse/human Arginase 1-eFluor 450 (1:300, 48-3697-82, Invitrogen). The Macrophage panel’s intracellular isotype antibodies consisted of Rat IgG2a kappa ...