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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... ELISA absorbance was measured at 450nm and normalized to total DNA content (SYBRGreen; Invitrogen) using an absorbance/fluorescence microplate reader (BioTek) ...
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bioRxiv - Immunology 2024Quote: Indirect ELISA was performed using Maxisorp 96-well microtiter plates (Thermo Scientific, Ref. 442404) coated with 50 µL of a 2 µg/mL solution of the rPvs48/45 protein solution overnight at 4°C ...
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bioRxiv - Biophysics 2021Quote: Plates were incubated with the secondary antibody dilutions for one hour at 37 °C and developed with 1-Step™ Ultra TBM-ELISA (Thermo Scientific™, Waltham, MA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Genomics 2021Quote: ... 50μg isotope-matched IgG and 50μl protein A/G agarose beads (A:G = 1:1, ThermoFisher 15918014 and ThermoFisher 15920010) and rotated at 4℃ for 8h to preclear the chromatin ...
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bioRxiv - Microbiology 2020Quote: ... and proteins detected using specific primary (diluted at 1:1000) and HRP-secondary antibodies (ThermoFisher, diluted at 1:10,000). Protein bands were detected using Pierce SuperSignal West Pico chemiluminescent substrate kit (Pierce ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Genomics 2020Quote: ... The DNA was captured on a 1:1 mixture of protein A and G Dynabeads (Invitrogen 10001D and 10003D). After washes ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1% Triton X in phosphate-buffered saline (phosphate-buffered saline (PBS)) and ReadyProbesTM Mouse-on-Mouse IgG Blocking Solution (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... To quantify the total soluble protein in each sample we used a bicinchoninic acid assay (BCA) following the protocol from Thermo Scientific (Pierce BCA Protein Assay Kit #23225). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Protein extraction and determination was performed using the B-PER™ Bacterial Protein Extraction Reagent and Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), respectively.
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Biophysics 2021Quote: The cDNA segment encoding the C1B domain of protein kinase C (PKC) δ isoenzyme from Rattus norvegicus (amino acids 229-281) was sub-cloned into pET SUMO expression vector (Invitrogen). The His6-SUMO-C1Bδ fusion protein was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: ... The removal of 3’ phosphates from fragmented RNA was performed using the Thermo Scientific™ T4 Polynucleotide Kinase (Thermo Fisher Scientific) and its supplied ATP-free buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... We also sequentially removed the 5’ and 3’ viroid moieties from this latter plasmid via PCR with the phosphorylated primers (T4 polynucleotide kinase, Thermo Scientific) D3606 and D3285 ...
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bioRxiv - Molecular Biology 2019Quote: ... Northern blot probes were labelled by incubating the RNA with 20 μCi of γ-32P-ATP (Hartmann Analytic) and 0.5 U/μl T4 Polynucleotide Kinase (PNK) in 1x PNK buffer A (Thermo Scientific) at 37 °C for 30-60 min ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Precipitated RNA was resuspended in 10µl of ultrapure water and 5µl was [γ-32P]ATP labelled by T4 polynucleotide kinase (Thermo Fisher Scientific) for 35 min at 37°C in buffer B ...
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bioRxiv - Cell Biology 2023Quote: Methods used for these assays have been described.28 Briefly, human FLAG-tagged CDKL5 WT or kinase dead (KD, CDKL5 K42R) constructs were subcloned into pT7CFE1-CHis plasmid (Thermo Fisher). These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Microbiology 2023Quote: ... 20 pmol of CIP-treated RNA was incubated with 20 μCi 32P-γ-ATP and T4 polynucleotide kinase (PNK, Thermo Scientific) in a 20 μl reaction system at 37°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG-tagged WT or kinase dead (KD, CDKL5 K42R) constructs of human CDKL5 were subcloned into a pT7CFE1-CHis plasmid (Thermo Fisher). A HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA ...
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bioRxiv - Developmental Biology 2020Quote: Total RNA was isolated from mouse embryos using the pico pure RNA isolation Kit (Thermo Fisher) and purified following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The IFN-β levels in BALF were determined using mouse IFN beta ProQuantum Immunoassay kit (ThermoFisher). IFN-α and IFN-β levels in the supernatants of cultured BMDCs were determined by ELISA (PBL Biomedical Laboratories).
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bioRxiv - Cancer Biology 2021Quote: ... BV711 anti-mouse Tim3) and LIVE/DEAD fixable Aqua dead cell stain kit (ThermoFisher, Cat. # L34957) at room temperature for 15 min followed by flow cytometry.
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bioRxiv - Neuroscience 2020Quote: Mouse brain and lungs were processed to isolate total RNA using PureLink RNA Mini Kit (Invitrogen). An equal amount of total RNA was used to synthesize cDNA with a High-Capacity cDNA reverse transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... The IFN-α/IFN-β 2-Plex Mouse ProcartaPlexTM Luminex Panel kit used was from Invitrogen. Tetramethylrhodamine ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from mouse saliva swabs using the PureLink™ Microbiome DNA Purification Kit (Invitrogen). ITS and FungiQuant qPCR primers [57 ...
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bioRxiv - Cell Biology 2020Quote: ... To generate mouse gapdh RNA probe the pTRI-GAPDH control construct (NorthernMax™-Gly kit, Ambion) was used as a DNA template for in vitro transcription ...
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bioRxiv - Cell Biology 2021Quote: LRRC23 was immunoprecipitated from mouse testis using the Pierce crosslink IP kit (Thermo Scientific, MA, USA) with anti-LRRC23 antibody described above ...
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bioRxiv - Neuroscience 2021Quote: ... OT-I CD8 T cells were isolated using a Dynabeads Untouched Mouse CD8 Cells Kit (Invitrogen). The purity of CD8 T cells was confirmed by flow cytometry (Fig S5) ...
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bioRxiv - Molecular Biology 2023Quote: ... AmpliSeq libraries were constructed using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific) that is designed for targeted amplification of over 20,000 mouse RefSeq genes simultaneously in a single primer pool ...
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bioRxiv - Physiology 2024Quote: ... and γ subunits and mouse prostasin were transcribed using the mMessage mMachine transcription kit (Life Technologies). Resulting cRNA encoding each of the subunits was injected into stage V to VI Xenopus oocytes ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell expressing protein fragments with an MBP tag were lysed first using 1 mL Bacterial Protein Extraction Reagent (B-PER; Thermo Scientific Catalog number: 78243) and 1-2x of protease inhibitor (Thermo Scientific A32963) ...
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bioRxiv - Neuroscience 2023Quote: ... The total protein concentration was determined by BCA assay and 500 μg of total proteins was incubated for 1 hour (4°C, rotary agitation) with 25 μl of Pierce™ Protein A Agarose beads (ThermoFisher, cat. no. 20333), centrifuged at 2500 g for 3 minutes at 4°C and the supernatant recovered as pre-cleared lysate ...
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bioRxiv - Genomics 2020Quote: ... and Protein A or Protein G Dynabeads (Invitrogen). After incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Protein reagent (Thermo Scientific PierceTM BCA Protein assay) was added to the supernatant and the standards and incubated for 30 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... or with Dynabeads protein A/protein G (Thermofisher) combined with Abatacept ...
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bioRxiv - Biochemistry 2021Quote: ... protein marker (NativeMark Unstained protein standard, LC0725, ThermoFisher) was diluted 50x in the working buffer (40 mM HEPES pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... protein A and protein G magnetic beads (Invitrogen) were added to capture the antibody-chromatin complexes ...
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bioRxiv - Immunology 2021Quote: ... For protein purification protein G dynabeads (Thermo Fisher), coupled with 2.5 µg of antibody (Table S8 ...
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bioRxiv - Neuroscience 2022Quote: ... Magentic dynabeads Protein G or Protein A (Invitrogen) were washed with 0.05% Triton X-100 in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein A and Protein G Dynabeads (Thermofisher Scientific) were washed twice with IP dilution buffer (150 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for two hours ...
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bioRxiv - Plant Biology 2022Quote: ... Magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were denatured using 1 x NuPAGE® LDS sample buffer (Thermo Fisher Scientific) and reduced with 2-Mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
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bioRxiv - Biochemistry 2020Quote: αT-catenin protein fragments were incubated with or without 1 mM BS3 (Thermo Scientific) in 20 mM HEPES ...
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bioRxiv - Microbiology 2020Quote: THP-1 cells were lysed in M-PER mammalian protein extraction reagent (Thermo Scientific) supplemented with Pierce protease inhibitor (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody is Rabbit polyclonal antibodies against green fluorescent protein (GFP) (1:500, Invitrogen). Secondary antibodies were conjugated with AlexaFluor-488 (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).