Labshake search
Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Polyclonal IgG H&L secondaries were Alexa Fluor 488- and 647-conjugates (Thermo Fisher #s A11029, A21235, A11008, and A21244) applied 1:500 in 10% ngs in PBST ...
-
bioRxiv - Neuroscience 2024Quote: ... the EdU detection was performed using the Alexa Fluor-647 Click-iT EdU Cell Proliferation Assay kit (Invitrogen Cat# C10424), following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2024Quote: Antibody-dependent cellular phagocytosis was assessed by conjugation of concentrated FL-RhCMV to Alexa Fluor 647 using NHS-ester reaction (Invitrogen), which was allowed to proceed in the dark with constant agitation for 1–2 h and then quenched by the addition of pH 8.0 Tris hydrochloric acid ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell cycle analysis was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen #C10634). Cells were incubated for 2 hours with 10uM EdU and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen #L34955 ...
-
bioRxiv - Cell Biology 2024Quote: ... we conducted cell-cycle analysis using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells seeded at a density of 5 × 105 cells per well in a 6-well plate were cultured for five days in StemFit AK02N media supplemented with Dox ...
-
bioRxiv - Cell Biology 2024Quote: ... EdU incorporation was determined with the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Invitrogen, C10419) according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2024Quote: WT IL-10 and Fab-IL-10 constructs were fluorescently labeled using Alexa Fluor 647 N-hydroxysuccinimide (NHS) ester (Invitrogen), and unreacted dye was removed with a Zeba Spin Desalting Column (7K MWCO ...
-
bioRxiv - Neuroscience 2023Quote: ... we incubated the retinas in secondary antibody diluted in PBST (1:800, Alexa Fluor 647-conjugated raised in donkey, Invitrogen) and Alexa Fluor 488-conjugated Phalloidin (1:100 ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... in 1% BSA in PBS for 1.5h and then with Alexa Fluor® 647 secondary antibody (1/250, Life Technologies) for 1h ...
-
bioRxiv - Developmental Biology 2023Quote: ... into newly synthesized proteins 42 using the Click-it Plus OPP Alexa Fluor 647 Proteinsynthesis-Assay-Kit according to the manufacturer’s manual (Thermo Fisher). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... Retinas were then incubated overnight in secondary antibody diluted in PBST (1:800, Alexa Fluor 647-conjugated raised in donkey, Invitrogen) and Alexa Fluor 488-conjugated Phalloidin (1:100 ...
-
bioRxiv - Molecular Biology 2023Quote: Cell cycle analysis was performed using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay kit (C10340, ThermoFisher Scientific). Cells were treated with 10 mM EdU and incubated for 30 min before being trypsinized ...
-
bioRxiv - Neuroscience 2023Quote: ... fluorescently conjugated to Alexa Fluor in one of three different wavelengths (488 nm, 555 nm, and 647 nm; C34775, C34776, C34778, respectively, Invitrogen). We made injections in 1-3 tracing locations per animal ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were washed twice in sorting buffer and then labeled with an Alexa Fluor 647-conjugated secondary antibody (1:100, Invitrogen) at 4°C for 30 minutes ...
-
Analysis of RyR2 distribution in HEK293 cells and mouse cardiac myocytes using 3D MINFLUX microscopybioRxiv - Physiology 2023Quote: ... samples were rinsed 3-4 times with PBS every 10 min and incubated with a 1:100 dilution of Alexa Fluor 647 secondary antibody (A-21235, Invitrogen) for 2h at RT ...
-
bioRxiv - Cancer Biology 2023Quote: Proliferation of cells was measured using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Invitrogen). Cells were treated with 10 μM EdU for 1 hour before they were harvested ...
-
bioRxiv - Bioengineering 2023Quote: ... samples were washed with 0.1% v/v Triton X-100 PBS and then incubated with the appropriate secondary antibody (1:200, Alexa Fluor 488 or 647, Invitrogen) and DAPI counterstain diluted in PBS overnight at 4° C on an orbital shaker ...
-
bioRxiv - Microbiology 2023Quote: Bacterial strains were inoculated into medium that contained 1 µM Alexa Fluor 647-labeled dextran (10,000 molecular weight; anionic, fixable; Invitrogen, D22914), added to Cellvis 12 well ...
-
bioRxiv - Cell Biology 2023Quote: ... The primary antibody was detected by a Fab fragment coupled to Alexa Fluor 647 (Thermo Fisher, A21246; dilution 1:500). The sample was then covered in imaging buffer (27 ...
-
bioRxiv - Cell Biology 2023Quote: ... EdU incorporation was visualised by using the Click-iT™ EdU Alexa Fluor™ 647 Imaging Kit (Invitrogen, Cat# C10340) prior to secondary antibody incubation.
-
bioRxiv - Cancer Biology 2022Quote: ... staining and the click chemistry reaction to detect EdU incorporation was carried out using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen), as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Click reaction was carried out with Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay kit (Thermo Fisher C10458) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Cells were then washed and stained with a 1:200 dilution of Alexa Fluor 647-labeled streptavidin (Thermo Fisher Scientific) in PBSA for 15 minutes at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... EdU signals were detected with the Click-it EdU Alexa Fluor 647 or 488 Imaging Kit (Invitrogen, C10340 or C10337). In brief ...
-
bioRxiv - Immunology 2023Quote: Antibody-dependent cellular phagocytosis was assessed by conjugation of concentrated RhCMV UCD52 to Alexa Fluor 647 using NHS-ester reaction (Invitrogen), which was allowed to proceed in the dark with constant agitation for 1–2 hours ...
-
bioRxiv - Developmental Biology 2023Quote: ... cells or sections were incubated with Alexa Fluor® 488 or 647-conjugated secondary antibody (1:500; Invitrogen, Eugene, OR) in carrier solution at room temperature for 1 hr ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were incubated with 10 μM EdU for 1 h using a Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (cat. no. C10419; Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: Cell cycle changes were analysed using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen, CA, USA). Cells were labeled with EdU for 3 h after drug treatment with THZ531 ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were fixed and the neurons visualized using Streptavidin-Alexa Fluor-647 conjugate (1:1000; Molecular Probes, Eugene, Oregon, USA) which was included with the secondary antibodies during immuno-labeling as outlined above ...
-
bioRxiv - Cell Biology 2023Quote: ... were applied at 1:100 dilution followed by secondary antibody conjugated to Alexa Fluor 488 or 594 or 647 (Invitrogen) at 1:100 ...
-
bioRxiv - Developmental Biology 2023Quote: ... paraffin sections were stained with Click-iT™ TUNEL Alexa Fluor™ 647 Imaging Assay (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s manual.
-
bioRxiv - Developmental Biology 2023Quote: ... paraffin sections were stained with Click-iT™ TUNEL Alexa Fluor™ 647 Imaging Assay (Molecular Probes, Thermo Fisher Scientific) according to the manufacturer’s manual.
-
bioRxiv - Cell Biology 2023Quote: ... Samples were then washed a minimum of five times (30 mins per wash) in PBT2 before incubating overnight in secondary antibodies (Alexa Fluor-555 and −647, 1:1000, Invitrogen) in PT2 at 4°C with gentle rocking in the dark ...
-
bioRxiv - Cell Biology 2023Quote: ... EdU signals were detected with the Click-it EdU Alexa Fluor 647 or 488 Imaging Kit (Invitrogen, C10340 or C10337). In brief ...
-
bioRxiv - Cancer Biology 2024Quote: ... we added an aliquot of barcoded reference cells stained with Alexa Fluor 647 Succinimidyl Ester (0.02 μg/μL) (ThermoFisher Scientific) to all samples ...
-
bioRxiv - Biophysics 2024Quote: ... The protein was subsequently labelled with a 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies) overnight at 4 °C with constant mixing ...
-
bioRxiv - Biophysics 2024Quote: ... replacing the caged dye in the HM with a small drop of 0.2-0.3 μl of a cytosolic fluorescent marker (3kD dextran conjugated to Alexa fluor 647 or Texas Red at concentrations of 3 mM; Invitrogen) that is applied from above just before electroporation to generate an inhomogeneous dye distribution around the animal during electroporation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were returned to the incubator for 24 hours and then processed for EdU incorporation by using a Click-iT EdU Alexa Fluor 647 flow cytometry kit (Invitrogen) and a LSR II flow cytometer (BD Biosciences) ...
-
bioRxiv - Cell Biology 2024Quote: ... Plasma membranes were stained with wheat germ agglutinin (WGA) conjugated with Alexa Fluor 647 (Cat. No. W32466, Life Technologies, USA) following manufacturer instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... Type and dilution of secondary antibodies was the same for vertical sections and whole-mounts (Alexa Fluor 488, 568, and 647, 1:500, Invitrogen; Alexa Fluor 405 ...
-
bioRxiv - Developmental Biology 2024Quote: Aortic SMCs isolated from pups at P0.5 were cultured with EdU (10 µM) from the Click-iT EdU Alexa Fluor 647 Imaging Kit (ThermoFisher, C10338) for 8 hours ...
-
bioRxiv - Immunology 2024Quote: ... 0.5 mls of Sm/RNPs (0.75 mg/mL) were labeled using the Alexa Fluor 647 Protein Labeling Kit (Invitrogen A20173) following the manufacturer’s instructions without removal of unbound AF647 ...
-
bioRxiv - Cell Biology 2020Quote: ... 594- and 647- conjugated goat anti-rabbit or anti-mouse secondary antibodies (Thermo Fisher Scientific). To visualize nuclear and cytoplasmic LDs ...
-
bioRxiv - Biochemistry 2020Quote: ... were recognized by secondary anti-rabbit or anti-mouse antibodies carrying AlexaFluor-647 labels (Invitrogen) and detected on the Typhoon scanner using the same module as above.
-
bioRxiv - Cancer Biology 2020Quote: ... anti-rabbit 488 or 568 and anti-mouse 647 (A11055, A21206, A10042, A21463, Molecular Probes). Confocal images were obtained using the same settings for the laser intensity and gain per experiment on a Carl Zeiss Confocal Microscope LSM 880 with Airyscan (Carl Zeiss ...
-
bioRxiv - Neuroscience 2024Quote: ... Secondary goat anti-mouse and anti-rabbit AlexaFluor 488 or 647 (Life Technologies, 1:1000).
-
bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 568 or Alex-Fluor 488 (Molecular Probes) for 1 h at rtp ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-Ig G rat conjugated with Alexa555 (Life technologies) and anti-Ig G rabbit conjugated with Alexa647 (Thermo Fisher) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and Alexa 598 goat anti-mouse/rabbit (Molecular Probes) were used as secondary antibodies ...
-
bioRxiv - Cancer Biology 2021Quote: ... and goat anti-mouse IgG1 Alexa 547 (Invitrogen, A21123) (1:100 ...