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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... followed by incubation for 2 h at room temperature with Alexa Fluor 680-linked anti-mouse IgG (A21058; Invitrogen, 1:5000). Signals were visualized with an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse anti-human PD-L1 antibody (cat. 14-5983-82) and goat anti-mouse IgG Alexa Fluor 488 conjugated antibody (cat. A11001) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... the tissues sections were washed with PBS and then incubated for 1 h at room temperature with the following secondary antibodies: anti-mouse Alexa Fluor 488 (1:600, ThermoFisher Scientific), anti-goat Alexa Fluor 555 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Slides were further incubated in blocking buffer for 5 min prior to adding the species-specific secondary antibody at room temperature overnight in a light-proof humidity chamber: Alexa fluor-546 anti-mouse (1:1000, Invitrogen, A11030), Alexa fluor-647 anti-rabbit (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed in PBS pH 7.2 and incubated for 1 hour at 35°C with secondary antibodies diluted 1:250 in PBS buffer with 1% BSA (Alexa Fluor 488-labeled mouse anti-rabbit IgG (Molecular Probes)).
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bioRxiv - Cell Biology 2023Quote: ... cells were washed in 1 x PBS then incubated with Alexa Fluor 488 goat anti-mouse (1:500, Thermo Fisher Scientific), Hoechst 33342 (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: Cells in the exo-erythrocytic development assays were subsequently stained with a primary antibody against Plasmodium berghei HSP70 (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and an anti-mouse Alexa Fluor 488 (1:300 diluted in 10% FCS/ PBS; Life technologies). Hoechst 33343 was added after for 5 min at RT and the cells washed twice in 1% FCS/ PBS the assays before being mounted ...
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bioRxiv - Cell Biology 2023Quote: Cells in the invasion assay were stained subsequently for 1 h at 37°C with primary mouse αCSP antibodies (cell culture supernatant 1:300 diluted in 10% FCS/ PBS) and anti-mouse Alexa Fluor 488 antibodies (1:300 diluted in 10% FCS/ PBS; Life technologies). Following two washing steps with 1% FCS/PBS the cells were permeabilized by addition of ice-cold methanol for 10 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Following secondary antibodies were used for immunofluorescent staining and super-resolution microscopy: anti-mouse Alexa Fluor® 532 (Life technologies, A11002), anti-rabbit (AffiniPure ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS by incubation with anti-mouse Alexa Fluor 488 plus (A32723, Thermo Scientific) or Alexa Fluor Plus 555 (A32773 ...
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bioRxiv - Biophysics 2024Quote: ... Cells were then washed three times with D-PBS (+/+) followed by incubation with Alexa Fluor™ 555 anti-mouse secondary antibody (#A-21422, Invitrogen) at a 1:1000 dilution for 1h at RT ...
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bioRxiv - Physiology 2024Quote: ... Alexa Fluor 594 donkey anti-rabbit (1:500) and Alexa Fluor 488 donkey anti-mouse (1: 500; Molecular Probes, Cat# A-21207. RRID: AB_141637, and Molecular Probes, Cat# A-21202 ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated at 37°C for 30 min with 40 μL of an Alexa Fluor 488-conjugated goat anti-mouse antibody (Life Technologies) diluted 1:500 in PBS 1× ...
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bioRxiv - Developmental Biology 2024Quote: ... The coverslips were then thoroughly washed with 1X PBS and the secondary antibody solution: donkey anti-mouse Alexa-Fluor 488 IgG (1:1000, Invitrogen A21202), donkey anti-rabbit Alexa-Fluor 555 IgG (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... and donkey anti-mouse antibody conjugated with Alexa Fluor 488 (catalog# A-212-2, RRID AB_141607, Thermo Fisher Scientific 1:500). To assess co-expression of ChAT and Chrimson ...
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bioRxiv - Biophysics 2024Quote: EDTA-containing D-PBS (blocking buffer)) and a goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary solution was prepared with a dilution of 1/100 of goat anti-mouse Alexa Fluor 700 (Invitrogen, A-21036), 1:100 of Alexa fluor 568 phalloidin (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The samples were then washed briefly in PBS prior to being incubated in corresponding secondary antibodies of Alexa Fluor 488 anti-mouse (1:500, Invitrogen, #A32766) or anti-rabbit (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... was used at a 1:300 dilution (gift from Benjamin Podbilewicz’s lab) with secondary antibody Alexa Fluor 488 donkey anti-mouse (Life technologies, #A21202) at a 1:500 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated with donkey anti-mouse IgG antibody conjugated to Alexa Fluor 488 (1:300; Thermo Fisher Scientific, RRID:AB_141607) and donkey anti-rabbit IgG antibody conjugated to Alexa Fluor 555 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... slides were washed with 1:3 diluted blocking buffer (3-times, 1 minute) and incubated with anti-mouse Alexa fluor-594 antibody (Invitrogen, A21203), anti-rabbit Alexa fluor-750 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.05 M TBS and then incubated in the dark for 2 h at room temperature with secondary antibodies: donkey anti-mouse Alexa Fluor 488 (3 µl/ml; Life Technologies) and donkey anti-rabbit Alexa Fluor 594 (5 µl/ml ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 1 hour at room temperature with the following secondary antibodies: (all 1:200 in 2.5% BGS-PHEM): Goat anti-mouse Alexa Fluor 488 (Invitrogen A A11001), goat anti-mouse Rhodamine Red (Jackson Immuno Research 115-295-003) ...
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bioRxiv - Neuroscience 2024Quote: ... and the secondary antibody used was a goat anti-mouse IgG (H+L) with either an Alexa Fluor 488 (1:250; Invitrogen A11029) or Alexa Fluor 647 congugate (1:250 ...
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bioRxiv - Genetics 2021Quote: ... Phalloidin conjugated with Alexa-568 and Alexa-647 (Sigma-Aldrich, Life Technologies) marks F-actin and were used (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa-647 or Alexa-568 coupled secondary antibodies (1:250, Molecular Probes) were used ...
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bioRxiv - Developmental Biology 2023Quote: ... Secondary donkey antibodies conjugated to Alexa 555 and Alexa 647 (Molecular Probes) were used at 1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa-555 or Alexa-647 (all 1:500, Invitrogen, Thermo Fisher Scientific) and the nuclear stain Hoechst 33258 (2 ug/mL ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa-568 (1:500; CB1R) and Alexa-647 (1:500; vGAT; Invitrogen, Grand Island ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa-555 or Alexa-647 for immunofluorescent staining were obtained from Invitrogen and used at 1:400 for ICC ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa 555 or Alexa 647 secondary antibodies (1/300 – 1/500, Invitrogen) that were incubated for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa-555 or Alexa-647 (all 1:500, Invitrogen, Thermo Fisher Scientific) and the nuclear stain Hoechst 33258 (2 ug/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested and processed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher Scientific #C10424) following manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Whole embryos were permeabilized with cold acetone for 7 minutes at −20°C and stained for EdU using the Click-it EdU Cell Proliferation kit for Imaging with Alexa Fluor 647 dye (ThermoFisher), as detailed in the kit protocol ...
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bioRxiv - Developmental Biology 2020Quote: Cell cycle phase distribution analysis was conducted using Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies) and FxCycle Violet (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... EdU incorporation assays were performed with the Click-iT® EdU Alexa Fluor® 647 Imaging Kit (C10339, Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... Four Hydra are placed in the chamber in 20μl of Hydra medium supplemented with 2mM of dye (Alexa Fluor 647-conjugated 10kD Dextran or Texas Red-conjugated 3kD Dextran, both from Invitrogen). A 150 Volts electric pulse is applied for 35ms ...
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bioRxiv - Neuroscience 2021Quote: Donor embryos were injected with 2.3 nl of 10 mg/ml Alexa Fluor 647 10 kDa Dextran (Thermo Fisher: D22914) at the 1-cell stage to distinguish them from host cells ...
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bioRxiv - Molecular Biology 2020Quote: ... was incorporated into cells on glass coverslips during 15 min at 37°C and revealed using the Click-It EdU Alexa Fluor 647 Imaging kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... EdU detection was performed using the Click-IT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies, C10634). Propidium (PI ...
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bioRxiv - Immunology 2021Quote: ... for assessment of edU incorporation we used the Alexa Fluor 647-azide using Click-iT Plus EdU Flow Cytometry Assay Kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... and either Alexa-Fluor 594-coupled Wheat Germ Agglutinin (WGA) for WT cells or Alexa-Fluor 647-coupled WGA for Fmr1-KO cells (5 μg/ml; Invitrogen) in D-PBS for 20 and 10 min ...
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bioRxiv - Zoology 2020Quote: ... After counterstaining with Alexa fluor-(488, 568 or 647) coupled-phalloidin (1:1000; Thermo Fisher Scientific or Santa Cruz Biotechnology) and DAPI at 2 mg/mL stock solution (1:500 ...
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bioRxiv - Pathology 2019Quote: ... the antibodies were developed with TSA following the manufacturer’s instructions with a solution of 1:100 Alexa Fluor 647 (AF647) Tyramide (B40958, Thermo Fisher) and 1:100 of 100x H2O2 in 1x tris-buffered saline for 15 minutes and was followed by PBST washes ...
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bioRxiv - Cell Biology 2019Quote: ... and stained at room temperature for 2 h with Alexa Fluor 647–conjugated soybean agglutinin (SBA; 100 µg/mL) (Invitrogen) and α-1,3-glucanase-α-1,3-glucan–binding domain fused with GFP (AGBD-GFP ...
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bioRxiv - Bioengineering 2019Quote: ... for 5 minutes at room temperature then washed thrice with DPBS before staining with 100 μL of 0.165 μM Alexa Fluor 647 Phalloidin (Thermo Fisher) supplemented with 0.25% Triton X-100 for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Staining was performed by Click-iT® EdU Alexa Fluor® 647 Imaging Kit following manufacturer’s instructions (Thermofisher scientific, USA). Confocal images were collected with an LSM-800 laser scanning confocal microscope with a 63x-oil Plan-Apochromat objective lens using ZEN Blue 2.6 software package (Carl Zeiss ...
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bioRxiv - Microbiology 2019Quote: ... Samples were incubated with a secondary antibody conjugated to Alexa Fluor 647 fluorophore at a dilution of 1:1000 (ThermoFisher) and DAPI at a dilution of 1 ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then washed three times in binding buffer by centrifugation and subsequently incubated with Alexa Fluor 647 streptavidin conjugate (Invitrogen) for 15 minutes at 4° C ...