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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... A similar endocytosis assay was used to observe transferrin uptake using 100 μg/mL of Alexa Fluor-647 (Catalog #T23366) or 568 (Catalog #T23365) conjugated transferrin (Invitrogen).
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bioRxiv - Bioengineering 2020Quote: ... Oligonucleotide-loaded sender cells were then stained with 5 µg/mL streptavidin-conjugated Alexa Fluor 647 (Thermo Fisher Scientific S32357) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2021Quote: ... The following secondary antibodies were used: Alexa Fluor-conjugated (405, 1:250, abcam; 488, 647, 568, 1:600, Molecular probes), Cy3-conjugated (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... then permeabilized with cold acetone for 10 min at −20 °C and stained for EdU using the Click-it EdU Cell Proliferation kit for Imaging with Alexa Fluor 647 dye (ThermoFisher), as detailed in the kit protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... EdU staining was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen, CatNo C10634) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... εdye and C280 for all the fluorophores used in this studies are so listed: Alexa Fluor 647 (Invitrogen, Cat# A20006): 650 nm ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... and MAGEA4 Ab (clone 57B, 4µg/mL) [39] previously coupled to Alexa Fluor 647 using Zenon labeling kit (Molecular Probes). Matched isotype antibodies were used as negative controls ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies conjugated with Alexa Fluor fluorescent proteins (488, 568, 647) were used in dilution of 1:200 (Molecular Probes no ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed the following morning and incubated for a minimum of 2h at RT with secondary antibody (1:1000, ant-rabbit Alexa Fluor-647, Invitrogen). Finally ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were permeabilised in 0.2% triton for 5 min and incubated with Click-it reaction cocktail (Click-it Alexa Fluor 647 C-10424 Invitrogen) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with PBS and detection was performed using an appropriate fluorescent secondary antibody (Alexa Fluor 488, 568, or 647, Invitrogen). Samples were mounted in DAPI containing antifade mounting medium ...
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained prior to CLMS with Hoechst 33342 (1:1000) and 100 µg/mL WGA Alexa Fluor 647 conjugate (ThermoFisher), a protein solution that binds to N-acetylglucosamine residues in peptidoglycan ...
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bioRxiv - Cancer Biology 2022Quote: ... we performed 5-ethynyl-2′-deoxyuridine (EdU) staining using the Click-iT EdU Alexa Fluor 647 imaging kit (C10340, Invitrogen). Four-color image acquisition of three replicates per condition was performed on an SP8 confocal microscope (Leica) ...
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bioRxiv - Bioengineering 2022Quote: ... Non-specific staining of the ECM was achieved by mixing the hydrogel precursor solution with 1% Alexa Fluor 647 NHS Ester (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... they were exposed to 200 nM rapamycin and 100 μg/ml Alexa Fluor 568 or 647-conjugated transferrin (Thermo Fisher) for the final 10 min of starvation ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed three times for 5 minutes with PBS before incubation with streptavidin with fluorescent tag (Alexa Fluor 647 – Thermofisher) in blocking solution at 1:1000 dilution for 1.5hrs ...
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bioRxiv - Bioengineering 2021Quote: ... The 640 nm laser (1000 mW peak power) was used at 40% setting to excite the reporter dye (Alexa Fluor 647, Invitrogen) and to switch it to the dark state and the 405 nm laser was gradually increased to reactivate the Alexa Fluor 647 in an activator dye (Alexa Fluor 405)–facilitated manner and maintain a constant density of active fluorophores ...
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bioRxiv - Developmental Biology 2022Quote: ... we performed a click chemistry-based EdU proliferation assay (Click-iT™ Plus EdU Alexa Fluor™ 647, C10640, ThermoFisher). We captured 5 random areas on each tissue section from each embryo ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell cycle was assessed using the same cells and conditions described above with Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay (ThermoFisher) according to the provided protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... EdU signals were detected with the Click-it EdU Alexa Fluor 647 or 488 Imaging Kit (Invitrogen, C10340 or C10337). Briefly ...
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bioRxiv - Physiology 2020Quote: ... cells were washed twice with ice-cold PBS on ice and incubated with EGF conjugated with Alexa-Fluor 647 (ThermoFisher) at a final concentration of 1 μg/ml for 1 h on ice taking precautions to protect from light ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were then rinsed in PBS and incubated in a fluorescent-conjugated streptavidin (Alexa Fluor-647, 1:1000, Invitrogen, UK) in PBS solution containing 3% NGS ...
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bioRxiv - Immunology 2020Quote: ... EdU and BrdU incorporation into DNA was assayed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen) and FITC BrdU Flow Kit (BD) ...
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bioRxiv - Pathology 2021Quote: ... Polyclonal IgG H&L secondaries were Alexa Fluor 488- and 647-conjugates (Thermo Fisher #s A11029, A21235, A11008, and A21244) applied 1:500 in 10% ngs in PBST.
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bioRxiv - Immunology 2021Quote: ... The RBD probe was prepared at a molar ratio of 4 moles of protein to 1 mole of Alexa Fluor 647-labeled streptavidin (Invitrogen). PBMCs from the five participants were thawed and stained for SARS-CoV-2-specific IgG+ memory B cells ...
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bioRxiv - Biophysics 2019Quote: ... and incubated in fluorescent secondary antibodies conjugated to Alexa Fluor 488 or 647 (Life Technologies, diluted 1:500 in AbDil) for 1 hour ...
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bioRxiv - Biophysics 2019Quote: ... The dye dilution was prepared from a stock solution that was made by dissolving Alexa Fluor 647 (A20006, Life Technologies) powder in DMSO (276855-100ML ...
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bioRxiv - Cell Biology 2020Quote: Fluorescent microtubules were polymerized from 4 mg/ml porcine tubulin (80% unlabeled and 20% Alexa Fluor 647 NHS ester-labeled; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... in 0.1 M sodium bicarbonate that was incubated with 98 μg of Alexa Fluor 647 succinimidyl ester (A20006, Thermofisher Scientific) for 1h at room temperature (3:1 dye:protein mole ratio) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with respective FITC- or Alexa Fluor 647-conjugated secondary antibody (Sigma-Aldrich F9137 or Thermo Scientific A21244) at a dilution of 1:100 for 1 h at room temperature ...
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bioRxiv - Pathology 2021Quote: Endothelial glycocalyx (eGCX) was labelled in vivo using wheat germ agglutinin (WGA) Alexa Fluor 647 conjugate (ThermoFisher, W32466, Waltham, MA) injected through the jugular vein (200 μl ...
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bioRxiv - Pathology 2021Quote: ... and the capillary basement membrane and pericytes were labelled with isolectin B4-Alexa Fluor 647 (1:200, overnight; Molecular Probes, I32450 ...
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bioRxiv - Microbiology 2021Quote: ... reovirus particles were diluted to 6×1012 particles/ml in freshly prepared 50 mM sodium bicarbonate buffer and incubated with 20 μM Alexa Fluor 647 succinimidyl ester dye (Invitrogen) at room temperature for 90 min ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected pregnant mice intraperitoneally with 100 μL of 5-ethynyl-2’-deoxyuridine (EdU, Click-iT EdU Alexa Fluor 647 Imaging Kit, Invitrogen) at 1mg/mL ...
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bioRxiv - Systems Biology 2021Quote: ... HEK293 transfected cells were stained with Hoechst 3342 nuclear stain and a wheat germ agglutinin (WGA, Invitrogen Alexa Fluor 647) plasma membrane (PM ...
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bioRxiv - Neuroscience 2021Quote: ... sections were labeled with a TH antibody (P60101) and incubated with Alexa Fluor 647 (Life Technologies, A-21448, 1:400). For aSyn neuropathology ...
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bioRxiv - Immunology 2020Quote: ... washed 2x with PBS 0.5% FCS and then stained with the Click-it Plus OPP Protein Synthesis Kit Alexa Fluor 647 (C10458, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Biophysics 2021Quote: ... The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies) overnight at 4 °C under constant gentle stirring ...
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bioRxiv - Biophysics 2021Quote: ... The A90C variant was labelled with 1.5-fold molar excess of C5 maleimide-linked Alexa Fluor 647 (Invitrogen Life Technologies) overnight at 4 °C under constant gentle stirring ...
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bioRxiv - Cell Biology 2021Quote: ... labeled for 20 min at RT with a 3.3 µg/µL solution of Wheat-Germ Agglutinin (WGA) Alexa Fluor©-647 nm conjugate (Invitrogen) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... EdU staining was performed with a Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher #10634), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated in Alexa Fluor 647-tagged donkey antirabbit IgG (1:500, Thermo Fisher, catalog # A-31573, RRID: AB_2536183) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... fluorescently conjugated to Alexa Fluor in one of three different wavelengths (488 nm, 555 nm, and 647 nm; C34775, C34776, C34778, respectively, Invitrogen). We made injections into 1-4 locations per animal ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell cycle analysis was performed using the Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Invitrogen #C10634). Cells were incubated for 2 hours with 10uM EdU and stained with LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Invitrogen #L34955 ...
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bioRxiv - Neuroscience 2022Quote: Cell-cycle experiments were based on the use of the Click-iT Alexa Fluor 647 imaging kit (Thermo Fisher C10340). Reagents were reconstituted according to the user manual ...
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bioRxiv - Cell Biology 2024Quote: ... we conducted cell-cycle analysis using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells seeded at a density of 5 × 105 cells per well in a 6-well plate were cultured for five days in StemFit AK02N media supplemented with Dox ...
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bioRxiv - Cell Biology 2024Quote: ... EdU incorporation was determined with the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (Invitrogen, C10419) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Polyclonal IgG H&L secondaries were Alexa Fluor 488- and 647-conjugates (Thermo Fisher #s A11029, A21235, A11008, and A21244) applied 1:500 in 10% ngs in PBST ...