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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the cells were incubated with a 1:1,000 dilution of goat anti-mouse IgG Alexa Fluor 488- conjugated secondary antibody (Thermo Fisher Scientific) in PBS for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... cells were incubated for 1 hour at room temperature with the following secondary antibodies: Goat anti-mouse Alexa Fluor 488 (Invitrogen, A11001), donkey anti-rabbit Rhodamine Red (Jackson Immuno Research 711-295-152) ...
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bioRxiv - Neuroscience 2021Quote: ... neurospheres were washed 3 times with PBS and incubated with secondary antibody goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) diluted at 1:1000 for 45 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed again in PBS and incubated with the respective secondary antibody Alexa Fluor 488 goat anti-mouse IgG (Molecular Probes) for L1 protein detection ...
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bioRxiv - Biochemistry 2021Quote: ... and a goat anti-mouse antibody conjugated with Alexa Fluor 488 (Life Technologies; 2 μg/ml in ND96 with 5% BSA) was applied for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with TBS and incubated with an Alexa Fluor 488 labeled goat anti-mouse antibody (1:500,Thermo Scientific) in blocking solution and were finally washed with TBS ...
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bioRxiv - Neuroscience 2020Quote: ... the brain sections were washed and incubated 1 hour with following secondary antibodies (Alexa Fluor 488 Goat anti-mouse IgG, Life technologies, #A11001/ Alexa Fluor 555 donkey anti-rabbit IgG ...
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bioRxiv - Neuroscience 2020Quote: ... Species-specific secondary antibodies were applied for two 60 minute incubations at 37°C as follows: Alexa Fluor 488-conjugated goat anti-mouse (IgG2a) at 1:1000 (#A21131, Life Technologies); Alexa Fluor 568-conjugated goat anti-mouse (IgG1 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibody binding was visualized using species specific fluorescent secondary antibodies conjugated to Alexa Fluor goat anti-mouse 488 (1:1000; Molecular Probes). Sections were mounted using fluorescent mounting medium (DAKO).
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bioRxiv - Biochemistry 2021Quote: ... 10 μg ml−1 diluted in 2% goat serum- and 2 mM EDTA-containing D-PBS (blocking buffer)) and a goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 2 h at RT with an Alexa Fluor 555 nm-conjugated goat anti-mouse secondary antibody (1:500; Molecular Probes, ThermoFisher Scientific cat#A21127 ...
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bioRxiv - Neuroscience 2022Quote: ... the labelled synaptosomes were incubated for 2 hours with secondary antibodies diluted in Tris TBS: Alexa fluor 488 Donkey anti-mouse IgG (1:500, Cat# A-21202: Invitrogen, RRID:AB_141607) and Alexa fluor 594 Donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were Alexa Fluor 488-conjugated Goat anti-Mouse IgG (1:500 dilution, Cat. No. A-11001, Thermofisher Scientific), Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (1:500 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-congugated antibodies were from Thermo Fisher Scientific.
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bioRxiv - Cell Biology 2019Quote: ... and then incubated with secondary antibody (1:500 anti-mouse Alexa Fluor-488; ex = 490 nm, em = 525 nm; Thermo Scientific) in PBT2 for 2 hours at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... and then stained at RT for 1 h in 50 µL of the 2nd antibody diluent (Alexa Fluor 546 Goat Anti-mouse IgG, A-11030, Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse IgG (H+L) (A-11001, 1:500; Molecular Probes), Alexa Fluor 555 goat anti-rabbit IgG (H+L ...
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bioRxiv - Synthetic Biology 2021Quote: ... as the primary antibody at a 1:100 dilution and anti-mouse IgG Alexa Fluor 488 as the secondary antibody (A11029, Life Technologies) at a 1:100 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and incubated for 2 hours at RT with Alexa Fluor 555 donkey anti-mouse (1:1000, Life Technologies-Invitrogen). Cells were finally stained with DAPI (1:5000 in PBS ...
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bioRxiv - Genomics 2019Quote: ... Samples were then washed three times in PBS for 5 minutes and incubated in secondary antibody (Alexa Fluor 488 goat anti-mouse, Invitrogen; R37120) for 30 minutes at room temperature ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... cells were blocked for one h with a 10% FBS solution and stained for GXM with mAb 18B7 (10µg/mL) as a primary Ab and “Alexa Fluor® 633 Goat Anti-Mouse IgG” (Life Technologies) as a secondary Ab ...
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bioRxiv - Immunology 2020Quote: ... tissue was incubated with 2 μg/mL BTX-488 to visualize AChRs and 2 μg/mL Alexa Fluor 594-conjugated donkey anti-mouse IgG (A21203, Thermo Fisher) in 2% BSA in PBS for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with PBS and then incubated with Alexa Fluor® 488F(ab’)2 goat anti-mouse IgG secondary (ThermoFisher) (1:300 in 2% human serum-PBS from a stock of 2 mg/ml ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were washed in PBT and then incubated with donkey anti-mouse secondary antibody (1:1000) coupled to Alexa Fluor 488 (Life Technologies) in PBT with 2% normal goat serum for 2 hours at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated with the secondary antibodies (1:1000, Goat anti-Mouse IgG secondary antibody, Alexa Fluor 488, Life technologies, USA) for 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Physiology 2019Quote: ... Cells were washed three times with PBS and incubated with goat anti-mouse Alexa Fluor-594 (Invitrogen, #A11005, 1:500 dilution) and donkey anti-rabbit Alexa-Fluor-488 (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated for 4 h at room temperature in fluorescent conjugated secondary antibodies (Alexa Fluor 488 goat anti-mouse (Th; 1:500; Invitrogen; A10667) and Alexa Fluor 594 goat anti-rabbit (DsRed ...
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bioRxiv - Microbiology 2019Quote: ... and secondary antibody solution was added (1-10ug/ml) (Alexa Fluor 488 Donkey anti-mouse IgG secondary antibody, ThermoFisher, A-21202). DAPI staining was used for nuclei ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated in absence of light for 1 h with fluorescent goat anti-mouse (1:500, Alexa Fluor 488, Life Technologies) diluted in TBS with 3% BSA ...
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bioRxiv - Microbiology 2020Quote: ... The antigens were then reacted with an Alexa Fluor 488-conjugated goat anti-mouse IgG H+L antibody (Thermo Fisher Scientific). The antibody titer was defined as the reciprocal of the highest dilution level at which a specific fluorescent signal was detected.
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bioRxiv - Molecular Biology 2020Quote: ... and washed extensively with PBS and finally stained with the secondary antibody conjugated with the appropriate fluorochrome (Alexa Fluor 488 goat anti-mouse supplied by Thermo Fisher, and Alexa Fluor 647 goat anti-rabbit supplied by Jackson Immunoresearch) ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Physiology 2021Quote: ... cells were incubated for 1 h at 37°C with secondary antibodies: Goat anti-Mouse Alexa Fluor 488 (ThermoFisher, A-11001) and Donkey anti-Rabbit Alexa Fluor 555 (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... prior to 1 hour incubation with secondary antibody at a 1:1000 dilution (Alexa Fluor 488 donkey anti-mouse, Life Technologies). A further 3 washes followed ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated for 1h at RT in a moist chamber with secondary antibody coupled to fluorochrome (Goat anti-mouse Alexa Fluor® 488 [A11001, Invitrogen] or 594 [A11005] and Goat anti-rabbit Alexa Fluor® 488 [A11008] or 594 [A11012] ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed five times in TBSN/BSA for one hr at 4°C whilst rotating and incubated with secondary antibodies overnight at 4°C at a dilution of 1:400 (Alexa Fluor 488 goat anti-mouse (Invitrogen, #A11001) and 1:400 Poly-HRP-conjugated secondary antibody from Tyramide SuperBoost Kits with Alexa Fluor 568 (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... brains were washed four times in PBST and incubated with the secondary goat antibody Alexa Fluor 633 anti-mouse (1:500 in PBST-NGS; Life Technologies) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The samples were finally incubated with Alexa Fluor 488-labeled donkey anti-mouse IgG secondary antibody (1:1000, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... After 2 washes cells were incubated for 1 hour with 0.5 μg/ml of Alexa Fluor 555 goat anti-mouse IgG (H+L) (ThermoFisher Scientific, #A21424). After 2 washes cells were resuspended in PBS and analyzed by flow cytometry using a FACS BD LSR-Fortessa.
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bioRxiv - Cancer Biology 2021Quote: ... Then coverslips were inverted onto 100 µL of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse - 1:200, Life Technologies #A11029 ...
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bioRxiv - Immunology 2021Quote: The density of IgG on cells was measured using Alexa Fluor 633 Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (ThermoFisher scientific). The secondary antibody was diluted 1:1000 in HBSS+0.1% FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Slides were washed again as described and then incubated with secondary donkey anti-mouse IgG (H+L) Alexa Fluor 594 (Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Immunology 2021Quote: ... bound antibodies were detected with an Alexa Fluor® 488-conjugated isotype-specific goat anti-mouse polyclonal serum (Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... three times and plates were incubated with secondary goat anti-mouse Alexa-Fluor-546-labeled antibody at 1:1,000 dilution (Thermo Fisher Scientific) for 2 h at room temperature in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... diluted in 1% BSA for 1 h at room temperature and then with goat anti-mouse IgG secondary antibody Alexa Fluor 488 (1:2,000; highly cross-adsorbed; Invitrogen, A-11029). The samples were finally immersed in ProLong Gold Antifade Mountant formulated with the DNA stain DAPI (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Secondary antibodies included Alexa Fluor IgG1 goat anti-mouse 568 (1:80 dilution, cat# A-21124, Life Technologies, Thermo Fisher Sci.) and Alexa Fluor goat anti-chicken 488 (1:250 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Secondary antibodies included Alexa Fluor IgG1 goat anti-mouse 568 (1:80 dilution, cat# A-21124, Life Technologies, Thermo Fisher Sci.) and Alexa Fluor goat anti-chicken 488 (1:250 dilution ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies). Immunolabelled coverslips were counterstained with DAPI (Sigma) ...