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Citations for Thermo Fisher :
8951 - 9000 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 200μL of platelet suspension (2×106 platelets in HBSSCa-Mg-) from healthy donors was labelled with calcein-AM (2 μg/mL final concentration, ThermoFisher Scientific Cat # C3100MP) for 15 mins and then R406 ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Immunology 2020Quote: ... Purified monoclonal antibody stock at a concentration of approximately 1mg/mL was 1:2 serially diluted in infection media (DMEM with 2% FBS and 1X penicillin-streptomycin (Thermo Fisher catalog 15140122) starting at 1:20 and ending at a dilution of 1:20,480 ...
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bioRxiv - Microbiology 2022Quote: ... and the residual bone marrow cells were plated in tissue-culture-treated microplates at a density of 2 × 105 cells per well in RPMI 1640 containing 2 mM glutamine (Thermo Fisher Scientific, 21875034), 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels with varying PEG-αMA wt% and DTT:MMP2-degradable crosslinker ratios (Table 1) were prepared with pH 8.0 N-2-hydroxyethylpiperaine-N-2-ethane sulfonic acid (HEPES; Thermo Fisher Scientific, Waltham, MA) as a solvent to allow base-catalyzed polymerization ...
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bioRxiv - Bioengineering 2022Quote: To assess endothelium barrier function we perfused microvessels with 2 μM Alexa Fluor647-conjugated 2 MDa dextran (Thermo Fisher Scientific, cat. no. D22914) in EGM-2 medium 48 h following inoculation with Bb or vehicle (BSK-II medium ...
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bioRxiv - Neuroscience 2022Quote: ... both proximal and distal chambers were washed with HibE imaging medium (Hibernate E [BrainBits] supplemented with 2% B-27, 2 mM GlutaMAX, 100 U/ml penicillin and 100 µg/ml streptomycin [Thermo Fisher Scientific/Gibco; 15140–122]) twice while maintaining fluidic isolation ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced every 2-3 days and cells passaged 1:2 or 1:3 weekly with 0.25% Trypsin/EDTA (Thermo Fisher Scientific, #25200-056) pre-warmed at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Microbiology 2023Quote: Vero E6 cells were transfected with 400 ng/well 2-E plasmids or 2-E mutations using Lipofectamine 3000 Transfection Reagent (L3000015, Thermo Fisher, MA, USA). The control group was Vero E6 cells transfected with 400 ng/well pcDNA3.1 vector plasmids alone ...
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bioRxiv - Developmental Biology 2023Quote: ... we washed the mature COCs in synthetic oviductal fluid medium (SOF) containing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES-TL, Thermofisher Scientific, Waltham, MA) and SOF for fertilization (SOF-FERT ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies (AlexaFluor546 anti-mouse, ThermoFisher, cat: A-11030, at 2 µg/mL; AlexaFluor647 anti-rabbit, ThermoFisher, cat: A32733, at 2 µg/mL) were incubated 2h/shaking at RT (BSA at 2% ...
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bioRxiv - Immunology 2024Quote: ... and SARS-CoV-2 S protein were immobilized at 2 μg/ml on 96-well polystyrene microtiter plates Nunc MaxiSorp™ (Thermo Fisher Scientific). Following blocking with 1 × synthetic block (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... tumors were cut into 2-4 mm3 pieces and resuspended in RPMI 1640 media supplemented with 2% FBS (Thermo Scientific, Waltham, MA, USA). These pieces then underwent mechanical and enzymatic digestion for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Biophysics 2021Quote: ... or a combination of pepsin and fungal protease type XIII (manually conjugated to POROS™ 20 AL Aldehyde Activated Resin, Thermo Fisher Scientific, and packed in a 2 mm ID x 2 cm IDEX C-130B column, Fisher Scientific, Hampton, NH), loaded on a C18 analytical column (Hypersil Gold ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies of strains were inoculated into 2 mL of minimal medium containing 2% succinate and 50 µg/mL kanamycin in 14 mL polypropylene culture tubes (Fisher Scientific, Waltham, MA, USA), then grown to mid-exponential growth phase with shaking at 200 rpm and 30 °C on an Innova 2300 platform shaker ...
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bioRxiv - Microbiology 2021Quote: ... The cell monolayers were then blocked with blocking buffer (2% Blotting-Grade Blocker [Bio-Rad] supplemented with 2% goat serum [Gibco] in PBS-Tween) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... iAT2s were serially passaged approximately every 2 weeks by dissociation into single cells via the sequential application of dispase (2 mg/mL; Thermo Fisher Scientific; 17105-04) and 0.05% trypsin (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Plant Biology 2022Quote: ... total soluble proteins were extracted from 50 mg of plant materials grown as indicated previously with 100 μL 2 x Laemmli buffer supplemented with 1% Phosphatase Inhibitor Cocktail 2 (Thermo Fisher Scientific Inc., 78430). Proteins were denatured for 10 min at 95°C and separated on 10% or 8% SDS-PAGE ...
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bioRxiv - Genomics 2022Quote: ... samples were incubated with primary Chr 2 intron probes for 30 hrs at 37°C in 50% Hybridization Buffer (2×SSC, 50% Formamide (v/v) (Thermo Fisher Scientific, Cat. AM9344), 10%(w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks and nasal washes were diluted 10-fold in 1× minimum essential medium (MEM) (10% 10× minimal essential medium [Gibco], 2 mM L-glutamine, 0.1% of sodium bicarbonate [wt/vol; Gibco], 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Developmental Biology 2023Quote: ... the corneas were cut into 2×2 mm pieces and sterilized by soaking in 1X Gibco phosphate buffered saline (PBS) (Fisher Scientific, 10-010-023) containing 1% HyClone penicillin streptomycin 100X solution (Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM DTT) with EDTA free protease inhibitor (Thermo Fisher) at 1:1 ratio (1 mL buffer ...
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bioRxiv - Cancer Biology 2021Quote: CaCo-2 and SW480 cells were cultured in DMEM (Gibco) supplemented with 10% foetal calf serum (PAN) ...
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bioRxiv - Biochemistry 2020Quote: ... 2 µl of Lipofectamine 2000 (Invitrogen, Carlsbad CA, 11668-019) was combined with 100 µl of Opti-MEM I (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2% B27 minus vitamin A (Gibco, 12587-010), 1x penicillin–streptomycin– glutamine (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 2% B27 minus vitamin A (Gibco, 12587-010), 1% penicillin–streptomycin–glutamine (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) staining for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 μM 2-β-mercaptoethanol (Gibco - catalogue no. 31350-010) and L-glutamine ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were passaged every 2 days using TrypLE Express (Gibco), and 3 million cells were plated per 10cm plate.
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bioRxiv - Cell Biology 2019Quote: ... followed by 2 hours incubation with Protein A dynabeads (Invitrogen). Immunoprecipitated material was sequentially washed with buffer containing low salt (150 mM NaCl) ...
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bioRxiv - Cell Biology 2020Quote: ... slides were stained in 2 % Giemsa solution (Thermo Scientific, #10092013) prepared in Gur buffer (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and 0.26% sodium bicarbonate (25080; Gibco).
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bioRxiv - Cell Biology 2019Quote: ... or 4 mL diamidino-2-phenylindole (DAPI; Thermo Scientific, 62248) in DPBS at a 1:1000 concentration ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-alanyl-L-glutamine (Thermo Fisher Scientific 35050079), 1× MEM nonessential amino acids (Thermo Fisher Scientific 11140076) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by selection with 2 μg/ml puromycin (Life technologies) for 2 weeks ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were biotinylated using EDC (Thermo Fisher) and Sulfo-NHS-LCLC biotin (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... and 2 mM Compound E onto polyethylenimine (PEI) (Thermofisher, AC178571000) and laminin-coated 24-well CytoView MEA plates (Axion Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... using iBlot 2 Dry Blotting System (Cat. # IB21001, Thermo Scientific). The membranes were blocked by 5% milk in PBST and probed by the specific primary antibodies at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... or gut organoids (n=2) were lysed with Trizol (Ambion) and RNA was extracted using the Direct-zol Miniprep kit (ZymoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mL of reduced serum Opti-MEM (Gibco, 31985088) according to the manufacturer’s directions ...
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bioRxiv - Neuroscience 2021Quote: ... 2°: goat anti-mouse AF488 (1:500) (Invitrogen, A-11001), and donkey anti-rabbit Cy5 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM glutamine and 100 U/ml penicillin/streptomycin (Invitrogen). For induction of apoptosis ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.1 mM 2-mercaptoethanol (Invitrogen, cat. No. 21985-023)] with freshly added 50 ng/ml Bmp4 (R&D Systems ...