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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested 6 days following transfection and passed through a 0.45 μm filter prior to purification using CaptureSelect™ CH1-XL (ThermoFisher) affinity chromatography.
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bioRxiv - Immunology 2021Quote: The RBD was conjugated to CuMVTT using the cross-linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, MA) at 10-molar excess for 60 minutes at 23°C ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Cell Biology 2021Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression analysis was then performed by Real Time quantitative PCR (qPCR) using a QuantStudio 6 Flex thermocycler (Thermo Fisher). We used the following Taqman primers from Thermo Fisher (Supplementary Table 3) ...
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bioRxiv - Immunology 2021Quote: ... 293T cells were transduced as outlined above while being seeded at 20% confluency on either 6-well or 96-well plates (ThermoFisher). For 6-well plates ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Neuroscience 2022Quote: ... or 300K cells per 6-well tissue culture plates were seeded in Microglia Differentiation medium (Advanced DMEM-F12 (Gibco, #12634010) + 1x N2 supplement (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064; 10 % horse serum, Gibco 26050-088 ...
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bioRxiv - Pathology 2022Quote: ... One million myoblasts per genotype and per mouse were pelleted at 300g for 5 minutes in 15 mL centrifuge tubes before pellets were transferred into individual wells of a 6-well plate containing 50 % proliferation medium (20 % foetal bovine serum, Gibco 10500-064 ...
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bioRxiv - Biochemistry 2022Quote: ... with a N-terminal FLAG-tag and a C-terminal 6×His-tag preceded by a two-residues linker were subcloned into pFastBac 1 vector (Gibco). Sequence transposition into bacmid DNA using DH10Bac cells (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time quantitative PCR was completed using the ThermoFisher Taqman Gene Expression Assays according to manufacturer’s protocol in the QuantStudio 6 Flex System (Applied Biosystems) mRNA targets included ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Cell Biology 2022Quote: ... Protein samples were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using the iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5% non-fat milk in TBS containing 0.1% Tween 20 (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using SYBR Select Master Mix and QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with the following parameter ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and the supernatant centrifuged twice at 1000g for 10 min at 4°C (Sorvall RC-6 Plus Centrifuge, Thermo Scientific). The supernatant was centrifuged 8000g for 10 min at 4°C and then aspirated without disturbing the pellet ...
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bioRxiv - Cell Biology 2022Quote: PBMCs were thawed in a 37°C water bath and plated into a 6-well plate (353046, Falcon) with RPMI media (11875-093, Gibco), supplemented with 10% fetal bovine serum (900-108 ...
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bioRxiv - Immunology 2022Quote: Bone marrow was harvested from the long bones of C57BL/6 mice by flushing with Hank’s Balanced Salt Solution (HBSS, Gibco, UK). Cells were cultured overnight in DMEM containing 10% fetal bovine serum ...
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bioRxiv - Genetics 2022Quote: ... and were seeded at 106 cells per well in neural expansion medium (NEM) in 6 well plates coated with Geltrex (A1413201, Gibco). This was considered as Passage 0 (P0) ...
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bioRxiv - Microbiology 2022Quote: Monocytes were isolated from the femurs of 6-12 week-old male C57BL/6 mice and differentiated into BMDMs for six days in 70% v/v RPMI 1640 medium (ATCC modification) (Gibco), 20% v/v L929 cell conditioned medium (provided by the Cell Services Science Technology Platform at the Francis Crick Institute) ...
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bioRxiv - Physiology 2022Quote: ... The FDB was rapidly dissected free and pinned to the bottom of a sylgard-coated dish containing collagenase type II (6 mg/ml, Gibco) in standard Tyrode’s solution ...
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bioRxiv - Neuroscience 2022Quote: ... qPCRs were run using PowerUp SYBR green master mix on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). Mouse primers used for qPCR:
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bioRxiv - Neuroscience 2022Quote: ... embryoid bodies were embedded in 15 µL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation media consisting of 1:1 DMEM-F12 and Neurobasal media (Gibco), with addition of 0.5% N2 supplement (Life Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were blotted for 6 s and plunged into liquid ethane using a vitrification apparatus (Vitrobot, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: LF82 infected and control cells were lysed at 6 hours post-infection (hpi) for 15 min in radioimmunoprecipitation assay (RIPA) lysis buffer (ThermoFisher) supplemented with protease inhibitor (Sigma ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed on an ABI Quant Studio™ 6 Flex system (Applied Biosystems, Waltham, MA, USA) with SYBR Green PCR master mix (TaKaRa ...
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bioRxiv - Plant Biology 2022Quote: ... and reactions were performed in eight-link boards on QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, USA) with cycling conditions (50 °C 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fugene HD transfection reagent and appropriate volume of Opti-MEM Reduced Serum Medium (31985070, Thermo Fisher, MA, USA) was prepared and incubated for 15 min at RT ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Microbiology 2022Quote: ... and P323L/G671S N-terminally tagged with 6×His were purified using Bac-to-Bac™ Baculovirus Expression System based on the manufacturer’s instruction (Gibco). Upon 3 dpi of NSP12-enconding baculoviruses into Sf9 cells ...
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bioRxiv - Microbiology 2022Quote: ... RT-PCR was performed in a 96-well plate using an Applied Biosystems QuantStudio 6 RT-PCR system (ThermoFisher Scientific). Reactions of 50 μLs in total volume were used which contained ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged once a week (∼80% confluence/well) at 1:4-1:6 ratios using 1mg/ml collagenase (Gibco) to detach colonies and onto fresh ir-MEF plates.
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bioRxiv - Neuroscience 2023Quote: Cultured neurons were incubated for 1 min with fluorescent dye N-(3-triethylammonium-propyl)-4-(6-(4-diethylamino)phenyl)-hexatrienyl)pyridinium dibromide (FM4-64; 4 µM; Invitrogen) and loaded by stimulation (10 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... Neuronal differentiations were carried out by plating 200,000 cells/12 well-well or 500,000 cells/6 well-well in DMEM/F12 base media (Gibco 11320-033) supplemented with B27 (Gibco 17504-044) ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed 6 times for 20 minutes and incubated in secondary antibody (Alexa Fluor 488 goat anti-chicken-488, #A11039, Invitrogen) diluted 1:200 in 10% normal goat serum for 3 hours in the dark at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 3 µg RNA for each sample were mixed with 6 µl of ERCC ExFold RNA spike-in mixes diluted to 1:100 (Invitrogen). Next ...
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bioRxiv - Immunology 2022Quote: ... primers are listed in Supplemental Table 3), Luminex using a custom procarta Luminex 6-plax-cytokines (IL1β, IL6, IL8, IL18, CCL2, and TNFa) detection kit (ThermoFisher) (Supplemental Fig ...
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bioRxiv - Genomics 2022Quote: ... For each transfection 1.6 ug of plasmid DNA has been incubated for 20 minutes with 4 µl of Lipofectamine 2000 transfection reagent (Invitrogen) in 0.2 ml of OptiMEM media (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were reconstituted in 6 uL 0.1% formic acid (FA) 0.05% heptafluorobutyric acid (HFBA) (Thermo Fisher Scientific, cat. 25003).
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bioRxiv - Neuroscience 2022Quote: ... in a final volume of 12.5 μL per reaction in 384-wells PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Physiology 2024Quote: ... Samples were heated at 65 ° for 6 minutes and quick chilled on ice prior to adding First strand buffer (Invitrogen) and DTT (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were replated at desired ratio (3.75 x 105 cells per well/of a 6-well plate) on Geltrex-coated wells in neuronal induction media: DMEM-F12 (Gibco) containing 1x N2 (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time qRT-PCR was performed on the QuantStudio 6 Flex thermocycler using PowerUp SYBR green master mix (ThermoFisher Scientific). All reactions were prepared according to the manufacturer instructions ...