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Citations for Thermo Fisher :
851 - 900 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentration in cell lysates was determined using Qubit™ Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration of cleared cell lysates was determined by BCA protein assay kit (Thermo Scientific #23225) and 20-30 μg of lysed samples were subjected to SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 mg of whole cell protein extract and 50 µl of Protein A Dynabeads (Invitrogen 10001D) were used ...
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bioRxiv - Molecular Biology 2019Quote: Total protein was extracted from cells using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, USA) supplemented with protease inhibitor cocktail (Roche Applied Science ...
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bioRxiv - Microbiology 2020Quote: Protein concentration from cell lysates were quantified by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific). Protein lysates were obtained by lysing cells in 0.1% SDS and protease inhibitor cocktail tablets (Roche #04693124001) ...
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bioRxiv - Microbiology 2023Quote: ... The total protein concentration in cell lysates was measured using a Bradford protein assay kit (ThermoFisher). Biofilm fractions isolated from cell culture supernatants were resuspended in PBS 0.2% Triton (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... The whole-cell protein concentrations are measured by the BCA protein assay (Pierce, Thermo Fisher Scientific). For SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) and purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270; Protein Free Hybridoma, Thermo Fisher, 12040077; penicillin and streptomycin, Thermo Fisher, 15140163) from day 6 up to day 18 from the start of differentiation ...
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bioRxiv - Cell Biology 2020Quote: The Subcellular Protein Fractionation Kit for cultured cells (ThermoFisher) was used to isolate the cytoplasm ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed in Expi293F cells (ThermoFisher). Five to 7 days post-transfection ...
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bioRxiv - Immunology 2021Quote: ... All proteins were transiently expressed in Expi293F cells (ThermoFisher). 5 to 7 days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were expressed in HEK293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Subcellular Protein Fractionation Kit for Cultured Cells (Fisher Scientific) was used and the protocol was executed through Step 5 to obtain the “membrane extract” ...
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bioRxiv - Microbiology 2020Quote: ... S2Pecto protein was expressed in FreeStyle 293 cells (ThermoFisher) or Expi293 cells (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Genetics 2020Quote: ... The protein was expressed in FreeStyle 293F cells (Invitrogen) grown in FreeStyle expression media ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface proteins in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... proteins were expressed in Expi293F cells (Thermo Fisher Scientific) at 37 °C and 8% CO2 ...
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bioRxiv - Immunology 2022Quote: Viral proteins were produced in HEK293F suspension cells (ThermoFisher) and purified as previously described 8 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: Total protein was isolated from cells using Trizol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were extracted using the Cell lysis buffer (Invitrogen). Seven micrograms of proteins were then stacked in the top of a 4-12% NuPAGE gel (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were expressed in Expi293F cells (Thermo Fisher Scientific) at 37 °C and 8% CO2 ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: Total protein was isolated from cells using Trizol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Microbiology 2023Quote: ... Cell protein lysates were quantified by Bradford assay (ThermoFisher), then separated by Tris-Glycine SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Cell Biology 2019Quote: ... then wet-blotted on X Cell 2 Mini Cell apparatus (Invitrogen GmbH) onto nitrocellulose membranes ...
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bioRxiv - Physiology 2019Quote: ... Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific, #1856209). Cell lysate was incubated with Anti-FLAG M2 antibody for 1 h and immunoprecipitated (IP ...
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bioRxiv - Bioengineering 2023Quote: ... was used for separation of the proteins by molecular weight and then separated proteins were transferred in iBot 2 PVDF membrane (Thermo Fisher Scientific, USA). Anti-Epo polyclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: Primary spinal microglia grown on poly D-lysine coated glass-bottom dishes were loaded with Fura-2 by incubating cells in 2 uM Fura-2-AM (Invitrogen) in growth medium for 30 min at 37℃ ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Virus-containing supernatant was harvested three days after transfection with Lipofectamine 2000 (Thermofisher). This supernatant was then cleared from cell debris by centrifugation at 3500 rpm for 15 min and filtered through a 0.45 µm filter (Millipore) ...
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bioRxiv - Neuroscience 2021Quote: ... to wash the virus in Opti-MEM™ Medium (ThermoFisher Scientific, Darmstadt, Germany) and concentrate to a final volume of 150 μl.
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bioRxiv - Neuroscience 2020Quote: ... They were reprogrammed into iPSCs using the replication incompetent Sendaï virus kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The virus pellet was then re-suspended in PBS (Thermo Fisher Scientific, 10010015) containing 25 mM HEPES buffer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For this the virus was diluted in 3 mL of 199 medium (Gibco). After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... using the Taqman Fast Virus 1-stepMaster Mix enzyme (Thermo Fisher, Waltham, MA). The limit of detection of this assay is estimated to be 200 genome equivalents/ml saliva or swab fluid ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Neuroscience 2020Quote: ... Cellular reprogramming experiments were conducted using the Sendai virus (CytoTune, Gibco, Life Technologies) carrying the four factors Oct4 ...
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bioRxiv - Microbiology 2021Quote: ... or Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a LightCycler 96 or LightCycler 480 instrument (Roche Diagnostics ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 1X TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific; 4444434) for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus reverse transcriptase (Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We used the TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) in a total reaction volume of 10 μl ...
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: ... ten-fold dilutions of virus were prepared in diluent medium (Opti-MEM (Invitrogen) with 2% FBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...