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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... for 30 minutes at 37°C (5 µM final concentration in insect medium) or H2DCFDA (Invitrogen #D399) at room temperature for 5 minutes (40 µM final concentration) ...
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bioRxiv - Cancer Biology 2019Quote: ... Drosophila egg chambers were dissected from whole ovaries and cultured in Schneider’s insect medium (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Millipore Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... hPolεCD(insect) with a cleavable N-terminal His-Tev tag was cloned into pFastBac-1 plasmid (Invitrogen). hPolεCD and hPolεCD(exo- ...
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bioRxiv - Molecular Biology 2019Quote: Salivary glands from early third instar larvae were dissected in insect media (HyClone SFX; Thermo Fisher Scientific), and fixed immediately with 4% PFA ...
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bioRxiv - Genetics 2020Quote: ... the embryos were transferred to a microcentrifuge tube filled with 500 µl of Schneider’s insect medium (Gibco). The blue pestle matching with the microcentrifuge tube (USA Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... S2p FHV and S2p FHV+DAV(33) were cultured at 25°C in Schneider’s Insect Medium (GIBCO), supplemented with 10% heat-inactivated fetal bovine serum (GIBCO) ...
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bioRxiv - Neuroscience 2023Quote: The brains of 7-day-old flies were dissected in Schneider’s insect medium (Thermo Fisher Scientific, 21720). They were then fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of three female insects were individually placed into tubes of TRIzol reagent (ThermoFisher, Waltham, MA) and frozen at −80° C for extraction of RNA ...
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bioRxiv - Microbiology 2024Quote: ... Liquid culture was in either BHI-NAD broth or Grace’s Insect Medium (Gibco™ Fisher Scientific, UK) [17] ...
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bioRxiv - Microbiology 2024Quote: ... Liquid culture was in either BHI-NAD broth or Grace’s Insect Medium (Gibco™ Fisher Scientific, UK) [17] ...
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bioRxiv - Molecular Biology 2020Quote: ... The suspension was incubated at 25°C for an additional 2 hr with Protein G SepharoseTM 4 Fast Flow or DynabeadsTM Protein G (Invitrogen), pre-blocked with 1 mg/ml BSA and 0.25 mg/ml Salmon Sperm DNA ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... 2% SDS and 10% Glycerol) and protein concentration was measured using Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). 20µg of protein was resuspended with SDS-loading dye (50% β-Mercaptoethanol and 0.02% Bromphenolblue) ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Bioengineering 2019Quote: iPSCs were reprogrammed from peripheral blood mononuclear cells using the Sendai virus approach (CytoTune-iPS 2.0 Sendai Reprogramming Kit - Thermo Fisher Scientific) according to the manufacturer instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... or empty pLKO.1-puro lentiviral vector were transfected together with pCMV-dR8.9 and vesicular stomatitis virus G–expressing plasmids into 293T cells using Lipofectamine 2000 (Thermo Fisher Scientific) for lentiviral supernatant production as previously described (48) ...
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bioRxiv - Microbiology 2021Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: All induced pluripotent stem cell (iPSC) lines were reprogrammed by the Sendai virus expressing 4 Yamanaka factors (CytoTune®-iPS Sendai Reprogramming Kit, Invitrogen) as previously described36 ...
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bioRxiv - Cancer Biology 2022Quote: ... except that some preparations were made using donor T cells that were activated for 48 hours before virus infection using LentiBoost (5 μl each of solutions A and B per ml of cells transfected; Fisher Scientific). 100 units/ml IL-2 was added at the start of the activation procedure and with every media change during the expansion phase.
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bioRxiv - Microbiology 2020Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM high glucose media (Life Technologies) with 0.7% low IgG BSA (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... was derived from Vero cells infected with the virus and cDNA was synthesized using the SuperScript III Reverse Transcriptase (Life Technologies) and oligo dT and six hexamer random primers ...
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bioRxiv - Microbiology 2020Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Virus containing supernatants were harvested 2 days later and the produced infectious virus yield was measured using TZM-bl reporter cell line and Gal-Screen kit from Applied Biosystems as recommended by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... and number of virus-infected cells (based on dsRNA and nucleocapsid staining) was performed using the CellInsight CX7 HCS platform (Thermo Fisher), as previously described 55,56 ...
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bioRxiv - Neuroscience 2022Quote: ... HT-1080 cells (ATCC CCL-121; RRID:CVCL_0317, male) used for virus titration were grown and maintained in MEM (Gibco Cat#11095-080) supplemented with 10% FBS ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Microbiology 2024Quote: ... Virus stocks were grown in M2-10B4 cells (ATCC # CRL-1972) with RPMI 1640 Medium special formulation (Thermo Fisher cat # A1049101) supplemented with 10% fetal bovine serum (Thermo Fisher cat # 12483020 ...
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bioRxiv - Microbiology 2021Quote: ... virus stocks were prepared in serum-free VP-SF media (Invitrogen). Viruses were harvested before CPE development ...
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bioRxiv - Molecular Biology 2021Quote: ... using the TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). According to the manufacturer’s instruction (QIAamp Viral RNA mini kit (Qiagen)) ...
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bioRxiv - Microbiology 2022Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 genomic RNA was amplified and detected using forward (5’- CGTGTAGTCTTTAATGGTGTTTCC-3’ ...
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bioRxiv - Molecular Biology 2020Quote: ... TaqMan™ Fast Virus 1-Step Master Mix (4444432, Life Technologies) was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were treated with 40 U/mL DNase I (Invitrogen) for 1 hr at 37°C before they were used to infect cells for 2 hr at an MOI of 0.5-2 ...
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bioRxiv - Physiology 2022Quote: ... and 200 U of Moloney murine leukemia virus reverse transcriptase (Invitrogen) in a final reaction volume of 20 μL (Evans-Molina et al. ...
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bioRxiv - Microbiology 2020Quote: ... using TaqMan Fast Virus 1-Step Master Mix chemistry (Applied Biosystems). SARS-CoV-2 N gene RNA was amplified using forward (5’-GACCCCAAAATCAGCGAAAT ...
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bioRxiv - Microbiology 2021Quote: Virus replication assays were performed in RPMI 1640 (Gibco 11875-093) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of TaqMan Fast Virus 1-Step Master Mix (ThermoFisher), and 9 μl of molecular-grade water per reaction ...
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bioRxiv - Immunology 2023Quote: ... Taqman Fast Virus 1-step kit (Thermo Fisher; catalog number 4444434) with oligos and probe specific to the N gene of SARS-CoV2 was used for SARS-CoV-2 RNA detection according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat No. 5555532) and a commercially prepared RNAse P primer/probe combination (IDT ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... virus inoculum was exchanged with serum-free MEM (Gibco, Life Technologies) containing 0.75% carboxymethylcellulose (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... dNTP and Moloney murine leukemia virus (M-MLV) reverse transcriptase (Invitrogen) were used to synthesize cDNA ...
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bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were normalized for protein content with Pierce 660nm Protein Assay (Thermo Scientific), boiled in NuPAGE LDS sample buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration of cell lysates were quantified by BCA Protein Assay (Thermofisher Scientific) according to manufacturer instructions and 50µg of cell lysate was mixed with Caspase-2 Activity Buffer (0.1 M MES [2-(N-morpholino ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysate protein quantification was measured by Pierce 660 nm Protein Assay (Invitrogen, 22662) and the EZQTM Protein Quantitation kit (Thermo ...
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bioRxiv - Immunology 2021Quote: ... Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific, U.S.A.) according to manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Protein concentration in cell pellets was determined by BCA Protein Assay Kit (Thermo Scientific). Ten microliter tissue homogenate is distributed into a 96-well plate and 200 µl ...
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bioRxiv - Cancer Biology 2023Quote: ... A protein digest from HeLa cells (Pierce HeLa Protein Digest Standard, Thermo Fisher Scientific) mixed with a standard peptide mixture (Pierce Peptide Retention Time Calibration Mixture ...