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Citations for Thermo Fisher :
951 - 1000 of 10000+ citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cDNA was prepared using Moloney Murine Leukemia Virus reverse transcriptase (Thermo Fisher Scientific) with Random Hexamers (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Nuclease treatment of virus stocks was performed at room temperature by DNase (Ambion), RNase (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... and Toledo virus was conjugated with DMSO-dissolved AF647–N-hydroxysuccinimide ester (Invitrogen) with constant agitation at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... the master mix used was TaqMan Fast Virus 1-Step Master Mix (ThermoFisher). Manufacturer’s protocol was followed to add the appropriate volumes of master mix ...
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bioRxiv - Microbiology 2022Quote: Purified virus particles were incubated with different concentrations of BS3 cosslinkers (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml, Gibco) 24h later ...
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bioRxiv - Neuroscience 2022Quote: ... PBMCs were expanded and reprogrammed using Sendai virus vector (CytoTune-iPS 2.0, Invitrogen) using a feeder-free system (Sommer et al. ...
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bioRxiv - Immunology 2023Quote: ... RNA from virus stocks was purified using TRIzol Reagent (Thermo Fisher Scientific, #15596026). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-containing media were filtered through a 0.45 µm filter (ThermoFisher Scientific) and mixed with fresh media at a 1:1 ratio before adding to pre-seeded target cells ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription was performed using Moloney murine leukemia virus reverse transcriptase (ThermoFisher Scientific). Real-time PCR was performed using TaqMan assays (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... U-2 OS cells were transduced with lentiviral vector encoding shBmal1 and cells selected using 2 μg/ml of puromycin (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: Cell surface protein biotinylation and isolation were performed using the Pierce™ Cell Surface Protein Biotinylation and Isolation Kit (Thermo Fisher Scientific, A44390) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... an internal solution containing 1 mM Fura-2 (K5-Fura-2, cell impermeable, Invitrogen F1200), 140 mM KCl ...
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bioRxiv - Biochemistry 2022Quote: ... Cells (70–80% confluent) were loaded with 2 μM Fura 2 AM (Molecular Probes, Oregon) in culture medium for 20 min at 37 °C in a humidified atmosphere of 95% O2 and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with 2 µg pcDNA3.1 CoV-2 Spike using Lipofectamine2000 (Thermo Fisher). At 24 hours post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a PVDF membrane using the iBlotTM 2 Gel Transfer system (Invitrogen). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... for 2 hours followed by 30 minutes incubation with protein A-coupled dynabeads (Invitrogen 10002D). Beads were washed three times with lysis buffer ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane using an iBLOT 2 dry blotting system (ThermoFisher) and analysed by immunoblotting using CALNEXIN (Bethyl A303-696A ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was run over two immobilized pepsin columns (Applied Biosystems; porosyme, 2-3131-00) stored at 10°C and 2°C at 200 µL/min for 3 min and the peptides were collected onto a VanGuard precolumn trap (Waters) ...
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bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...
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bioRxiv - Genomics 2022Quote: ... Mix 2 μg antibody with 20 μL Dynabeads Protein A beads (ThermoFisher Scientific, Cat. 10001D) with rotation at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and 55 nM of 2-Mercaptoethanol) with the protein transport inhibitor Brefeldin A (Thermo Fisher) added during the last 4 hrs of incubation at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were reduced and alkylated with 25 mM Tris(2-carboxyethyl)phosphine (TCEP) (Thermo Fisher) and 50 mM chloroacetamide (CAM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF iBLOT 2 Transfer Stacks according to manufacturer’s instructions (ThermoFisher Scientific). Membranes were blocked in 5% non-fat milk with 0.1% tween in PBS (PBS-T) ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 2 mg of protein was mixed with 1x SUPERase·In™ RNase inhibitor (Invitrogen™) and incubated with Dynabeads IgG (11203D ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Plant Biology 2019Quote: ... A total of 2 µg of PageRuler Plus Prestained Protein Ladder (#26 619, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2021Quote: The protein samples were treated with Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher Scientific; # 20491) and 2-chloroacetamide (cat# 154955 ...
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bioRxiv - Immunology 2021Quote: ... immune complexes were incubated with magnetic beads for 2 h (Protein G Dynabeads, Thermo Fisher Scientific or Protein A/G magnetic beads ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then further digested with 2 μg MS-grade trypsin (Thermo Fisher Scientific) per 100 μg protein for 20 hours at 37 degrees Celsius and 850 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with mouse anti-Bcl-2 antibody (Thermo Fisher Scientific, MA511757, 1:100), rabbit anti-HMGB1 antibody (Cell Signaling ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were affinity-purified by incubation with 2 mL of Ni-NTA agarose resin (Invitrogen) for 30 minutes at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The precipitated proteins were resuspended in 2 mL of 1.2% SDS in 1xPBS (Life Technologies) by pipetting ...
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bioRxiv - Molecular Biology 2022Quote: ... and protein transfer was achieved using the iBlot™ 2 Gel Horizontal Transfer Device (Thermofisher). Nitrocellulose membranes were blocked in 5% BSA in PBS/Tween 20 (0.1% ...
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bioRxiv - Molecular Biology 2022Quote: ... AEBSF 1 mM) with 2 µg of antibodies and Dynabeads Protein G (Thermo Fisher Scientific). Control immunoprecipitation (IP ...
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bioRxiv - Microbiology 2024Quote: ... followed by a further 2 hours with Protein Transport Inhibitor Cocktail (Invitrogen: 00-4980-03). Cells were analyzed for degranulation and cytokine production by flow cytometry.
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bioRxiv - Neuroscience 2024Quote: ... 25 pmol of sgRNA was complexed with 2 µg of TrueCut Cas9 Protein v2 (Invitrogen) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Protein was transferred to a PVDF membrane using the iBlot 2 system (Thermo Fisher, IB21001). Western blotting was performed with rabbit anti-Cx43 (1:5000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to PVDF membrane using the Iblot 2 system (Thermo Fisher Scientific IB21001). Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco ...
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bioRxiv - Cell Biology 2023Quote: ... Reduction of proteins was achieved with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Thermo Fisher) for 30 min and samples were subsequently alkylated in 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and - 2 for E.coli were prepared using Pierce HeLa Protein Digest Standard (Thermo Fisher Scientific), MS Compatible Yeast Protein Extract ...
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bioRxiv - Cell Biology 2023Quote: Reconstitute Human FGF-basic (FGF-2/bFGF) Recombinant Protein (here we use ThermoFisher 13256-029). Briefly ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to PVDF using an iBlot 2 dry blotting system (Thermo Fisher), blocked in 3% milk + 2% BSA and immunoblotted overnight at 4 °C with antibodies specific to Arabidopsis FLS2 (Agrisera) ...
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bioRxiv - Biochemistry 2023Quote: ... SARS-CoV-2 spike variant proteins in PBS pH7.4 were immobilised on Maxisorb ELISA (Nunc) plates at a concentration of 2 μg/mL overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg/ml of antibody and 20 μL/mL of Protein A Dynabeads (Invitrogen, 10002D) were added and incubated overnight at 4°C on a rotating wheel ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to nitrocellulose membranes using iBlot 2 (Invitrogen). After blocking for one hour ...