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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 5% glycerol and then mixed at 9 volumes to 1 volume of 200× concentrate SYPRO orange (Thermo Fisher) diluted in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... intestinal fragments were incubated in epithelial cell solution (10 mM EDTA [Invitrogen, #15575020]-HBSS, 5 mM HEPES [Invitrogen, #15630080] ...
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bioRxiv - Immunology 2023Quote: ... These cells were maintained at 37□°C in a 5% CO2 air atmosphere in DMEM (Gibco, Cat; 12430054) containing 10% foetal bovine serum (FBS ...
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bioRxiv - Biophysics 2023Quote: ... Cells were cultured at 37°C with 5% CO2 in homemade phenol red containing DMEM (Thermo Fisher #12800082) with 4.5 g/L glucose supplemented with 10% fetal bovine serum (HyClone ...
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Deciphering the gene regulatory circuitry governing chemoresistance in Triple-Negative Breast CancerbioRxiv - Cancer Biology 2023Quote: ... siRNA at a final concentration of 5 pmol was diluted in 45 μL of Opti-MEM (Gibco, 31985047) and 2.25 μl of Lipofectamine RNAiMAX was diluted in 45 μl of OPTI-MEM ...
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bioRxiv - Microbiology 2023Quote: ... 293T/ACE2 cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, WAKO) containing 10% fetal bovine serum (Gibco) and penicillin/streptomycin (100 U/ml ...
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bioRxiv - Molecular Biology 2022Quote: The qRT-PCR assays were performed using the QuantStudio™ 5 Real-Time PCR System (ThermoFisher Scientific, USA) with the following cycling conditions for CHIKV ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in RPMI 1640 + Glutamax supplemented with filter-sterilized and heat inactivated 10% fetal calf serum (Gibco), 50 U/ml Penicillin (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: Peptides were analyzed with 5 different MS methods on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific), summarized in Supplementary Dataset S7 ...
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bioRxiv - Neuroscience 2023Quote: ... and then washed with PBS 3 x 5 min and mounted with ProLong Gold Antifade Mountant (ThermoFisher, P36930). Mounted coverslips were then imaged on a Zeiss Axio Observer-Z1 widefield microscope equipped with a Zeiss Plan-Apochromat 63x / 1.4 Oil objective ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... 5% CO2 using either DMEM (HeLa and PANC-1: high glucose, GlutaMAX™ Supplement, pyruvate; ThermoFisher Scientific 10569010) or RPMI 1640 (Jurkat and PBMC ...
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bioRxiv - Genomics 2023Quote: Murine J774.2 macrophages were maintained at 37°C under 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 mM EDTA) containing 250 mM NaCl and Halt Protease and Phosphatase Inhibitor Cocktail (78445, Thermo Fisher Scientific) for 30 min at 4°C with rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Bioengineering 2023Quote: ... and then blocking buffer was added (5% normal goat serum [ThermoFisher], 0.2% Triton™ X-100 [Fisher Scientific] ...
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bioRxiv - Cell Biology 2023Quote: ... The blotted membrane was immersed in block buffer [5% milk (AppliChem # A0830.1000) in 1XTBS (TBS, Thermo Fisher #28358)] for 2 hr at RT ...
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bioRxiv - Biochemistry 2023Quote: ... FAM122AID and FAM67-120 and variants were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Bioengineering 2023Quote: ... and the fluorescence signals were traced by the quantitative real-time PCR QuantStudio 5 (Thermo Fisher Scientific, USA) with λex = 495nm and λem = 520nm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slides were again washed with PBS-/- 3×5 min and mounted using Prolong Gold Antifade Mountant (Thermo Scientific). Sections stained with secondary only antibodies were included to determine fluorescence threshold before image acquisition in each channel ...
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bioRxiv - Biochemistry 2023Quote: ... temperature-dependent protein unfolding profiles were measured using the QuantStudio™ 5 realtime PCR machine (Thermo Fisher Scientific). Excitation and emission filters were set to 465 nm and 590 nm ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST) (Thermo Scientific, J77500.K2). The membranes were blocked for 1 h at RT with 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... sections were first blocked and permeabilized in a 5% normal goat serum (Thermo Fisher Scientific, cat. no 10000C) and 0.5% Triton X-100 (Carl Roth ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblots were incubated for 5 minutes at room temperature with Supersignal West Femto maximum sensitivity substrates (Thermo Scientific) and developed using an ImageQuant 500 CCD imaging system (Cytiva).
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bioRxiv - Microbiology 2023Quote: ... The reactions were then run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA).
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bioRxiv - Molecular Biology 2023Quote: ... All cells were grown at 37°C with 5% CO2 and in Dulbecco’s modified Eagle’s medium (Gibco, 11995065) supplemented with 10% fetal bovine serum and penicillin-streptomycin-glutamine (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with the same medium with the addition of 5 μg mL−1 zeocin (ThermoFisher Scientific). For neuronal maturation ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (Thermo Scientific SuperSignal West) and imaged using a BioRad Chemidoc.
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bioRxiv - Microbiology 2023Quote: ... and HEK293T cells were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2023Quote: Freshly isolated neutrophils were stained with CellTracker™ Green 5-chloromethylfluorescein diacetate (CMFDA) dye (Thermo Fisher scientific, USA) at 5µM in serum free RPMI-1460 (SFM ...
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bioRxiv - Genomics 2023Quote: ... Moran) were cultured at 37°C and 5% CO2 in HeLa complete medium (DMEM, Life Technologies, Cat# 11960044) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... cells were cultured at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium with glutamine (DMEM) (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... or for 30 min with 5 µM CellROX™ Green Flow Cytometry Assay Solution (Thermo Fisher Scientific, C10492) in fresh DMEM/F12+Glutamax w/o FBS and P/S ...
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bioRxiv - Neuroscience 2023Quote: ... For IHC we permeabilized and blocked sections with blocking solution (PBS containing 5% normal goat serum [Thermo Fisher Scientific # 10000C] and 0.1% Triton X-100 [Millipore-Sigma # X100-100ML] ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells (ATCC) were grown in a 37°C and 5% CO2 atmosphere in DMEM (Gibco Cat# 11995065) + 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... Cells were then blocked with 2 mL blocking solution made of 5% Normal Goat Serum (cat#PCN5000 Thermofisher) and 0.05% Triton X-100 in PBS for 2 hours at RT or 4°C on a shaker.
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bioRxiv - Genomics 2023Quote: ... Real-time PCR was performed using a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA). The PCR cycling conditions were as follows ...
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bioRxiv - Microbiology 2023Quote: ... and pYK-JRCSF HIV-1 expression plasmid in a 5:1 mass ratio with Lipofectamine 2000 (Life Technologies) according to the manufacturer’s manual.
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bioRxiv - Molecular Biology 2023Quote: ... Each ATO pellet was resuspended in 5 µL of RB-27 medium comprising of RPMI 1640 (Thermofisher Scientific), 4% B-27 supplement (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The cell supernatants were discarded at 5 h after seeding and replaced with Neurobasal plus Medium (Gibco, A3582901) containing 2% B-27 Plus (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... authenticated using STR profiling) were cultivated in DMEM supplemented with 5 % horse serum (Thermo Fisher Scientific, cat.no. 16050122), 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were thawed at room temperature and washed three times for 5 minutes in DPBS (Gibco, 14190–094). Sections were then counterstained for 5 minutes with Hoechst 33342 (1μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Peritoneal Lavage cells were centrifuged at 1200 rpm for 5 min and resuspended in alpha MEM (Gibco; 12571063) media supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... A final wash in PBS for 5 mins was carried out before mounting in ProLong Gold (Invitrogen, P36930). Slides were stored at −20°C until imaged.
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... then washed 3x for 5-10min with PBST and mounted in ProLong Antifade Mounting medium (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... were cultured at 37 °C under a 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... for 5 min followed by destaining with Tris-buffered saline-0.05% Tween 20 (TBST) (Thermo Scientific, J77500.K2). The membranes were blocked for 1 h at RT with 5% bovine serum albumin (BSA ...