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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...
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bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Coverslips were counterstained with 20 nM Hoechst 33342 for 5 min in Cell Staining Buffer (Thermo Fisher Scientific) then mounted on slides with Vectashield Antifade Mounting Medium (Vector laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 µl of a 300 µM intermediate concentration of DAPI (Invitrogen by Thermo Fisher Scientific, Cat. no.: D1306) was added per mL of sample ...
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bioRxiv - Cell Biology 2023Quote: ... followed by two 5-min washing steps with washing buffer B (2X SSC and 10% formamide, Ambion; AM9342). In a humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell experiments were performed in RPMI or DMEM media supplemented with 5% charcoal stripped FBS (cFBS) (Gibco) as a method of removing substantial amounts of lipophilic materials from the media [17 ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor [ThermoFisher]) and lysed by high- pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets expressing PP2Aa9-589 were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin C3-Emulsiflex) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets expressing EGFP_Nanobody were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR reactions were carried out on a Real-time PCR machine (QuantStudio 5 by Applied Biosystems), according to a protocol from the Bimake SYBY Green Master Mix qPCR Kit (B21402) ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of mid-exponential growth phase cells (OD600 of 0.6-0.8) was mixed with 5 µg/ml Nile red (Invitrogen) and 1 µg/ml Hoechst 33342 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was precipitated overnight at −20 °C with 0.025 V of 5 mg/mL glycogen (ThermoFisher Scientific), 0.1 V of 3.0 M NaOAc (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was precipitated at −20 °C overnight with 0.025 V of 5 mg/mL glycogen (ThermoFisher Scientific), 0.1 V of 1.0 M NaOAc (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were reduced and alkylated in the same step with 5 mM TCEP (Thermo Fisher Scientific, PG82080) and 20 mM chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed in 50 µL system including 10 µL 5× HF buffer (Thermo Fisher Scientific, Vilnius, Lithuania), 1 µL dNTPs (10 mM ...
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bioRxiv - Immunology 2023Quote: ELISA plates (Microtitre) were coated with 50 µl/well of 5 µg/ml NeutrAvidin (Thermo Fisher Scientific, #31000) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Microbiology 2023Quote: ... The native genomic locus of Bacillus cereus B4264 Shedu (NCBI accession NC_011725.1, bases 954720-956931) 5 was synthesized (GeneArt/Invitrogen) and cloned into pSG1 vector between AscI and NotI sites ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Pathology 2023Quote: ... PrP-deficient RK13 cells (ATCC) were transfected with 5-10 µg of plasmid DNA using lipofectamine 3000 (Invitrogen). At 24 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded and concentrated on PepMap C18 precolumns (length, 5 mm; inner diameter, 0.3 mm; Thermo Scientific) at a flow rate of 30 µl/min and separated using Acclaim PepMap C18 reversed-phase nano-LC columns (length ...
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bioRxiv - Biochemistry 2023Quote: ... The dialysed protein was loaded onto a column packed with HisPur Ni-NTA Agarose Resin (5 mL, ThermoFisher) equilibrated with binding buffer (50 mM Tris HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... HS-5 cells or primary MSCs were cultured in DMEM medium supplemented with 10% FBS (Thermo Fisher Scientific), 1 % penicillin/streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Treatment with the vehicle hydroxypropyl-beta-cyclodextrin (HPBCD, Acros Organics; 5% w/w in water for injection (WFI)) ...
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bioRxiv - Cell Biology 2023Quote: ... or siNIPBL or 5 nM of siAQR was reverse transfected into the cells using Lipofectamine RNAiMAX reagent (Invitrogen), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Culture conditions included incubation in 5% CO2 at 37°C and passaging every 72 hours with trypsin (Gibco). Cells were never allowed to reach confluency.
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bioRxiv - Cell Biology 2022Quote: ... 5–10mg of protein per sample was run on a 4–12% NuPAGE Bis-Tris Gel (ThermoFisher Scientific) and then transferred to PVDF membrane by liquid transfer using NuPAGE Transfer buffer (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher) through gold coated capillary needles that were prepared in-house30 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: All qPCR reactions were carried out in a 96-well plate on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Cell Biology 2022Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC-MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with spectral DAPI for 5 minutes and mounted with ProLong Gold antifade reagent (Life Technologies). The slides were imaged on the Pannoramic 250 Flash II digital whole slide scanner ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cells were stained with 5 μM of CellTrace Violet Cell Proliferation Kit (Thermo Fisher Scientific, C34557), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were then resuspended in 5 mL of red blood cell lysis buffer (ThermoFisher, 00-4333-57) and incubated for 5 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... After 24 h or 72 h incubation at 37 °C and 5% CO2 in the incubator (Cytomat, ThermoFisher), 25 µl CellTiterGlo® (Promega ...
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bioRxiv - Cell Biology 2023Quote: Embryos were incubated for 1hr at 28ºC with 400 µM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen Cat# A5040) and 5% DMSO in E3/PTU ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed with the cell impermeable bissulfosuccinimidyl suberate (BS3) crosslinker at 5 mM (21580, Thermo Scientific, USA) for half hour at room temperature followed by quenching with 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... SEM images were acquired on a FIB-SEM platform (Helios 5 UX DualBeam Fisher Scientific, Brno, Czech Republic) using SEM imaging mode at 1.8 kV and 0.1 nA using TLD detector.
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bioRxiv - Evolutionary Biology 2023Quote: ... were used to transfect 5×105 Sf9 cells in 1ml of SF900 II SFM growth medium (ThermoFisher scientific). The complex was incubated for 45 min/RT and the lipid complexes were added dropwise to the appropriate well and were incubated at 27°C for 6 hours ...
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bioRxiv - Genomics 2023Quote: ... 5 µl of diluted cDNA was mixed with 1xAbsolute Blue qPCR SYBR green low ROX mix (ThermoFisher, AB4322B) and 500 nM primer concentration ...
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bioRxiv - Immunology 2023Quote: ... and SAv-Alexa Fluor 488 were mixed together with 5 µM free D-biotin (B20656; Thermo Fisher Scientific) to minimize cross-reactivity between probes ...
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bioRxiv - Immunology 2023Quote: ... pRL-CMV-Renilla (5 ng) and pReceiver-M12-SH2B3 (100 ng) plasmids using Lipofectamine 2000 (Invitrogen; cat # 11668019) resuspended in Opti-MEM (Gibco ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... 5 g of tissue from brd-8V5 were purified with V5 antibody (Thermo Pierce)-conjugated Dynabeads (Life Technologies). A single protein band or the whole lane87 were cut off from the gel as indicated in the figure and used for mass spectrometry.
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bioRxiv - Developmental Biology 2023Quote: ... 5 mM EDTA + PIC) was incubated with 25 μl of pre-washed Dynabeads Protein G (10004D, Thermo Fisher) and with 4 µg of the antibody of interest overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: Target 786-O cells were trypsinized and labeled with Calcein-AM (5 μmol/L, catalog no. C1430, Invitrogen) for 30 minutes at 37°C in dark ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were passaged in clumps at ∼ 70% confluency every 3 – 5 days by dissociation with Versene (Life Technologies) at typical ratios between 1:6 and 1:12 ...