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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with different GIPR constructs using Lipofectamine 2000 transfection reagent (Invitrogen). All the mutant constructs were modified by single-point mutation in the setting of the WT construct (HA-Flag-3GSA-GIPR(22-466)) ...
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... HUVEC seeded at 1.5 × 106 cells per well of a collagen-coated 6-well pate were transfected with plasmids using the Neon Transfection System (Invitrogen), harvested 48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on coverslips coated with poly-L-lysine in 6-well plates and cultured in Neurobasal medium (Gibco) supplemented with B27 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted from 1 confluent well of a 6-well plate using RNA PureLink RNA mini kit (Life Technologies) as per manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... The mice were tail vein-injected with 100 μL of 6 mg/mL Hoechst dye (Thermo Fisher Scientific, Waltham, MA) 4-6 h prior to termination of an experiment to visualize nuclei (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng of nLYN-mCherry and 500 ng of iLID plasmid were mixed with 2.1 ul (1 to 3 ratio) of Fugene 6 in 125 ul of Opti-MEM medium (Thermofisher Scientific). After 10 minutes incubation at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... and the efficiency of rRNA depletion was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Assays were performed in analytical triplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Warrington, Cheshire, UK) and target gene expression levels were normalized to average expression of housekeeping gene GAPDH using ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were transfected with 0.25 μg/6 × 104 Hela cells of the myc-Rac1Q61L plasmid with turbofect (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Biochemistry 2021Quote: ... were seeded in 6-well plate with a cover glass inside and were transfected using lipofectamine 2000 (Thermo Fisher, #11668027) with mouse mEGFP M-Lip or mEGFP M-Lipxtal and mApple-Sec61b ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... MEFs were seeded at a density of 200 000 cells per well of a 6-well plate in MEF media consisting of DMEM 1x (41965-039, Invitrogen) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2020Quote: Cells were grown in 6-well culture dishes to 70-80% confluency and RNA was isolated with TRIzol reagent (#15596-026, Ambion), DNase I treated using the RNase free DNase kit (#79254 ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Cell Biology 2019Quote: ... De-epidermized dermis were then placed at the bottom of a 6-well plate in 3D-HRPE culture medium composed of IMDM medium (Invitrogen) and keratinocytes medium (CellSystems ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from HIEs wells (in hW-CMGF+ or differentiation media for 4 days) or MA104 cells grown to confluency in a 6-well plate using TRIzol reagent (Ambion). Total RNA was treated with Turbo DNase I (Ambion ...
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bioRxiv - Molecular Biology 2019Quote: ... 20ng of totalRNA was used for library preparation with ERCC Spike-in control Mix A (Ambion 10-6 final dilution). All steps were performed on a Tecan EVO150 ...
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bioRxiv - Genomics 2019Quote: ... A real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (# 4485694, Applied Biosystems, CA, USA) with the following reagents ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... 5 μl of each fraction (polysomes and non polysomes) was used for First-strand synthesis using p(dN)6 and 100 U of M-MLV RT (Invitrogen). The same amount of first strand DNA was used for qRT-PCR analysis ...
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bioRxiv - Genetics 2020Quote: ... we reverse transfected approximately 3.0 x 105 - 5.0 x 105 293T/TLR cells with 75 nM of siRNA per well of a 6-well plate using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Abi3-WT and -KO MØPs were maintained in 6-well plates with Roswell Park Memorial Institute 1640 (RPMI; Gibco®, ThermoFisher) supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were grown on coverslips in 6 well dishes and transfected with pcDNA3.1-3XFLAG-ZNF507 plasmid using lipofectamine reagent (Thermo Fisher). After 36 hours cells were fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... with 1.6 µM of specific primers with an QuantStudio™ 6 Flex system (Applied, Biosystems, Life Technologies, Saint-Aubin, France) in a final volume of 15µL ...
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bioRxiv - Cell Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... and primers and a FAM-ZEN/Iowa Black FQ labeled probe sequence (IDT) on the QuantStudio 6 system (Applied Biosystems). An Ultramer DNA oligo (IDT ...
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections were prepared from whole ovaries at 6 µm thickness and placed on charged glass slides (Fisher Scientific). Ovary sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T cells were seeded onto 6-well culture plates and transiently transfected with different PTH2R constructs using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h ...
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bioRxiv - Developmental Biology 2020Quote: Mouse small intestinal organoids derived from the duodenum of C57BL/6 mice were cultured in ENR medium: Advanced DMEM/F12 (Invitrogen) with 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Microbiology 2021Quote: ... After 7 days of differentiation the BMDMs were seeded at a density of 0.8×106 cells/well in 6-well plates in MGM 10/10 media containing Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco®) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-FU-D131-#6) were labeled by 15 min incubation with 1 μM CFSE (CellTrace CFSE, ThermoFisher Scientific, Ref: C34554). Cells were then resuspended in PBS supplemented with 30% Percoll ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated onto 6-well plates and transfected the following day (70-80% confluence) using Lipofectamine 2000 (LF2000) (Invitrogen) for 4 h in DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2019Quote: ... in the iPSC media or in a feeder-free condition in StemFit AK02N (AJINOMOTO) in 6-cm dishes coated with 0.5 μg/cm2 vitronectin (Life Technologies). The cells were expanded using glass capillaries manually ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Molecular Biology 2021Quote: ... or stress granules (G3BP1 antibody, Supplementary File 6) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 400 ng of each RNA was used following supplier’s recommendations and carried out on a Quant 6 RT PCR (Applied Biosystems). Gene expression data was analyzed by excel and normalized to the mock transfected values ...
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bioRxiv - Immunology 2021Quote: ... Real time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). Raw Ct values were first normalized using the inter-plate calibrator UniSp3 ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... Protein samples either from ESCs or liver extracts were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5 % nonfat milk in PBS containing 0.1% Tween 20 (PBST) ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...