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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.7 mM dNTPs with 10 U SuperScript II reverse transcriptase (Invitrogen, 18064022). The obtained cDNA products were purified using Mag-Bind Total Pure NGS beads (Omega Bio-Tek ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The cells were counted using Countess II Automated Cell Counter (ThermoFisher Scientific). The total live cell count/ml was recorded as the final number of cells and the growth rate was calculated as Nf = N0 2ηt ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from isolated RNA using SuperScript II Reverse Transcriptase (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... reactions were stopped by adding 12 µL of loading buffer II (Ambion). manY mRNA transcripts were incubated at 90 °C for 5 minutes in alkaline buffer (Ambion ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and synthesized cDNA from the resulting mRNA with SuperScript II RT (Invitrogen), using a primer containing a unique molecular identifier (UMI ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA was reverse transcribed with Superscript II (Invitrogen). Real time PCR was performed using primers 5’-ACGTGATGAGGAGGTTCT-3’ (sense ...
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bioRxiv - Developmental Biology 2020Quote: ... First strand cDNA synthesis was carried out using SuperScript II RT (Invitrogen). Quantitative real-time PCR primers were designed so that one of each primer pair was exon/exon boundary spanning to ensure that only mature mRNA was amplified ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was reverse transcribed to cDNA using SuperScript II Reverse Transcriptase (Invitrogen) using the provided random hexamer primers ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were counted using the Countess II FL Automated Cell Counter (ThermoFisher). Routine light microscopy was performed using the EVOS FL Cell Imaging System (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... elegans cells (eg. Bio-Rad TC20™ and Invitrogen Countess II™) were empirically found to be about an order of magnitude lower than the cell counts obtained using FACS ...
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bioRxiv - Cancer Biology 2019Quote: ... Resulting cDNA was analysed using TaqMan Universal Master Mix II (Life technologies). Gene expression was determined using an Applied Biosystems 7300 Real-Time PCR system by the comparative ΔΔCT method ...
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bioRxiv - Immunology 2020Quote: ... following the protocol of SuperScript II reverse transcriptase (Thermo Fisher Scientific #18064014). Primers were designed against Rora isoform ENSMUST00000113624 (fwd ...
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bioRxiv - Molecular Biology 2019Quote: Sf21 cells (Thermo Fischer) were cultured in SF900 II SFM (Thermo Fisher), supplemented with 10% heat-inactivated Fetal Bovine Serum (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... and cDNA was prepared using random hexamer oligonucleotides and Superscript II (Invitrogen). Primers used for qPCR analysis are listed in Table S9.
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized with Super Script II Reverse Transcriptase (Thermo Fisher; 18064014), followed by end repair ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixture contained 1X Accuprime PCR buffer II (Invitrogen, Carlsbad, CA), 1 unit Accuprime HiFi taq ...
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bioRxiv - Physiology 2019Quote: ... riboprobes were cloned into PCR-II TOPO (Thermo Fisher Scientific, Massachusetts, USA) or PGEM-T Easy Vector (Promega ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cloned into pDONR207 with the GatewayTM BP Clonase II Enzyme Mix (Invitrogen) and transferred to pUBN-GFP-DEST using the GatewayTM LR Clonase II Enzyme Mix (Invitrogen).
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bioRxiv - Plant Biology 2019Quote: ... BiFC pairs were generated by using Multisite LR clonase II plus (Invitrogen) with each reaction containing the desired pDONR221 donor construct pairs and a pBiFC 2-in1 plasmid (3:3:1 molar ratio ...
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bioRxiv - Immunology 2019Quote: ... cDNA from whole islets and BMDMs was generated using Superscript II (Invitrogen). cDNA from FACS-sorted cells was prepared using Superscript III (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... for 30 min followed by incubation with 0.2% collagenase type II (Gibco) in PBS for 20 min ...
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bioRxiv - Biochemistry 2019Quote: Cells were plated on Lab-Tek II 8-chamber slides (Thermo Fisher) and incubated as described in the figure legends ...
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bioRxiv - Molecular Biology 2019Quote: ... products were amplified with Phusion HS II DNA polymerase (Thermo Fisher F549), and Gibson Assembly Master Mix (New England Biolabs E5510 ...
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bioRxiv - Synthetic Biology 2019Quote: ... The purified PCR product was ligated into pCR-Blunt II TOPO (Invitrogen) following the manufacturer’s instructions and transformed into chemically competent Stellar E ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was resuspended in ~10 μL Gel Loading Buffer II (Invitrogen) and subjected to denaturing PAGE electrophoresis on a 20% Urea-PAGE gel ...
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bioRxiv - Genomics 2020Quote: ... Cell viability was assessed via Trypan blue staining (Countess II, ThermoFisher #C10228), with >90% viability required for both PRO-seq and ChIP-seq experiments.
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bioRxiv - Genomics 2020Quote: ... BP reaction was performed using the BP Clonase II enzyme mix (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were maintained in Sf-900 II serum free medium (Gibco) and High Five cells were maintained in ESF 921 Insect Cell Culture Medium (Expression System) ...
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bioRxiv - Genetics 2020Quote: ... Radioactive probes were prepared with a DECAprime II DNA Labeling Kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Add 1 ul of 200 U SuperScript™ II RT (#18064014, Invitrogen) for a final volume of 20 uL ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA from rat DRG was reverse transcribed with Superscript II (Invitrogen) and then amplified by PCR using PrimeStar HS polymerase (Takara) ...
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bioRxiv - Genetics 2019Quote: ... and used to synthesize random-primed cDNA (SuperScript II Reverse Transcriptase, Invitrogen). A Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies ...
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bioRxiv - Biophysics 2021Quote: Sf9 cells were cultured in serum-free medium (SF900 II, Thermo Fisher) at 27°C with a speed of 130 rpm in a shaker (Yonglian) ...
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bioRxiv - Genomics 2020Quote: ... using Gateway® LR Clonase® II Enzyme mix (Invitrogen, ThermoFisher Scientific), following manufacturer’s instructions.
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bioRxiv - Genomics 2020Quote: ... using Gateway® LR Clonase® II Enzyme mix (Invitrogen, ThermoFisher Scientific), following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized from isolated RNA using SuperScript II Reverse Transcriptase (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 2% hematocrit in 1640 RPMI-HEPES supplemented with 5% AlbuMAX II (GIBCO) and 0.25% gentamycin complemented with appropriate Artemisia infusion dilutions and with or with IPP and Cm ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was performed with SuperScript™ II Reverse Transcriptase (18064022, Invitrogen) with random hexamers primer ...
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bioRxiv - Neuroscience 2020Quote: ... First strand cDNA was synthesized with SuperScript II Reverse Transcriptase kit (Invitrogen) following manufacturer instructions used with random primer mix ...
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bioRxiv - Biochemistry 2021Quote: Transfected INS832/13 cells were cultured in LabTek-II coverglass chambers (Nunc). At 24 h post-transfection live cell fluorescence was examined using a Nikon A1 Confocal Microscope ...
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bioRxiv - Bioengineering 2020Quote: ... Real-time PCR was performed using TaqMan Universal Master Mix II (ThermoFisher) along with the following TaqMan primers ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA synthesis reactions were performed with Superscript II Reverse Transcriptase (Life Technologies) kit following with random priming and manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Sf9 cells were obtained from Thermo Fisher Scientific and grown in Sf-900 II SFM media (Thermo Fisher Scientific). All cells were routinely tested for mycoplasma contamination and were not authenticated after purchase.
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bioRxiv - Cancer Biology 2020Quote: ... RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized with Super Script II Reverse Transcriptase (Thermo Fisher; 18064014), followed by end repair ...
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bioRxiv - Cell Biology 2021Quote: ... reverse transcription with 2 ng RNA was performed using SuperScript II (Invitrogen) in the presence of oligo-dT30VN (1µM ...
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bioRxiv - Neuroscience 2020Quote: ... For the first strand synthesis Super Script II RT was used (Invitrogen). Taqman Fast Universal PCR solution was mixed with TaqMan probe with an Applied Biosystems FAM dye ...
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bioRxiv - Biochemistry 2020Quote: Gateway LR Clonase II Enzyme Mix (Fisher Scientific, Cat. # 11-791-020)
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bioRxiv - Cell Biology 2021Quote: ... using a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... using a Gateway adapted vector using BP/LR Clonase II (Thermo Scientific) according to the manufacturer’s instructions.