Labshake search
Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
-
bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
-
bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
-
bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
-
bioRxiv - Genetics 2019Quote: ... using 1 μl PCR product in 9.5 μl Hi-Di™ formamide containing 0.5 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
-
bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
-
bioRxiv - Bioengineering 2020Quote: The Taq polymerase-amplified PCR product of the rdLRP was inserted into the TOPO Cloning site of a pcDNA3.1/V5-His-TOPO vector (Thermo Fisher Scientific, MA). The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: The full-length OpIE2 promoter was obtained from the pIB/V5-His by PCR using Phusion High fidelity DNA Taq polymerase (ThermoFisher Scientific, USA) according to the following program ...
-
bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
-
bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
-
bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
-
bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 μl of fivefold-diluted PCR product was mixed with 9.25 μl of Hi-Di formamide and 0.25 μl LIZ600 size standard (ThermoFisher Scientific, Waltham, MA, USA), denatured for 10 min at 95°C and then immediately cooled on ice ...
-
bioRxiv - Cell Biology 2019Quote: ... and FLAG-dDYNLL1 (EcoRI/XbaI) fragments were inserted into the S2-cell expression vector pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020).
-
bioRxiv - Molecular Biology 2019Quote: ... using 1 μl PCR product in 9.6 μl Hi-Di™ formamide containing 0.4 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
-
bioRxiv - Microbiology 2019Quote: ... The lysates were run on a 15% PAGE and developed using monoclonal anti-His antibody (Biospecs, BTL1010) with super signal west femto maximum sensitivity substrate (Thermo Scientific, 34095).
-
bioRxiv - Genetics 2020Quote: The PCR products were diluted 1:1 with milliQ water and 1μL of the diluted PCR product was added to a 384-format genotyping plate containing 4μL of a mix of Hi-Di™ formamide (Applied Biosystems™) and Genescan™ 600 LIZ™ dye size standard v2.0 (Applied Biosystems™ ...
-
bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...
-
bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... and T2/Onc2.r1 5’-ATC TAT GGC TCG TAC TCT ATA GGC-3’ designed to amplify the pT2/Onc2 vector excluding the MSCV 5’ LTR minimal promoter and Lun-SD sequences using platinum Hi-Fi DNA Taq polymerase (Invitrogen #11304-028) in 1X Hi-Fi PCR buffer (Invitrogen #52045 ...
-
bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then plated in 96-well flat bottom plates at a ratio of 25:75 with CD45.1 splenocytes and stimulated in 10% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) containing Brefeldin A (eBioscience cat ...
-
bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
-
bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
-
bioRxiv - Immunology 2022Quote: ... The libraries were sequenced on Ion Proton using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite Software v5.2.2 (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2024Quote: ... and Esr2b (XM_020713365) cDNA was amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Each nucleotide sequence of the 5′-flanking region of ara and arb was retrieved from the Ensembl medaka genome assembly and analyzed for potential canonical ERE-like sequences using Jaspar (version 5.0_alpha ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
-
bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
-
bioRxiv - Microbiology 2023Quote: The Lysobacter enzymogenes gasdermin and the Pedobacter rhizosphaerae system were both expressed under an arabinose-inducible PBAD promoter in the pBad/His A plasmid (Thermo Scientific V43001). The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The lysate was cleared by centrifugation at 20,000 x g for 30 min at 4 °C and purified by using GE Healthcare His GraviTrap™ Kit (Cat. No. 45-002-016, Fisher Scientific). After elution ...
-
bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
-
bioRxiv - Cell Biology 2022Quote: ... Antibodies against ZNF524 were purified and enriched from the serum against this recombinantly expressed His-ZNF524 using the SulfoLink® Immobilization Kit for Peptides (Thermo Scientific) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2023Quote: ... were expanded and maintained in MEMα (GibcoTM 22561021) supplemented with 10% heat-inactivated FBS (HI-FBS; LabTech FB-1001/500 or Gibco 10438-026) at 37°C ...
-
bioRxiv - Developmental Biology 2024Quote: pMT-Reps-Flag and pMT-Reps-HA were generated by cloning Reps-RA isoform from FMO04419 (DGRC Stock 1612020) into pMT-V5-His vector (Invitrogen cat#V412020). pMT-Mnb-V5 was generated by cloning Mnb-RH isoform from FMO12028 (DGRC Stock 1639183 ...
-
bioRxiv - Biophysics 2024Quote: ... Lysates containing Utrophin or α-actinin were loaded on a column packed with His-Pur Ni-NTA resin (ThermoFisher Scientific, Waltham, MA). The column was washed with 0.6 M NaCl ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
-
bioRxiv - Molecular Biology 2024Quote: The piggyBac piRNA target site was inserted into the middle of the V5 coding sequence in the pIB-V5/His vector (Thermo Fisher Scientific) by site-directed mutagenesis ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a 1.5 hrs protein-chromatin pull-down with a 1:1 mix of protein A and protein G Dynabeads (Thermo Fisher; 10001D and 10003D). ChIP samples were washed extensively ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNA in the protein samples was sheared by sonication and the amount of protein was estimated using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were separated on 4–20% Mini-PROTEAN® TGX™ Gels (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™, PI23227). The desirable amount of the protein lysate (15 – 30 μg ...
-
bioRxiv - Microbiology 2022Quote: ... The EBOVLP input was normalized based on the concentration of EBOV structural proteins as determined with a bicinchoninic acid (BCA) protein assay (Pierce™ BCA protein assay kit, Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: Protein lysate prepared as described above (750 µg for an over-expressed protein) was precleared with 30 µL of protein G or protein A agarose beads (Roche # 10042392 and Invitrogen # 15918-014, respectively) for one hour ...
-
bioRxiv - Plant Biology 2019Quote: ... UAS∷GAD2Δ and ALMT9 using Phire Hot Start II DNA Polymerase (Invitrogen) with primer sets:
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription was performed using the SuperScript II Reverse Transcriptase kit (Thermofisher). RT-qPCR was performed using Fast SYBR Green Master Mix (Thermofisher ...
-
bioRxiv - Cancer Biology 2021Quote: ... using Gateway™ LR Clonase™ II Enzyme mix (Invitrogen, 11791-020), forming the vector pINDUCER20-PPARGC1A-3xFLAG.
-
bioRxiv - Cell Biology 2019Quote: ... 0.5% trypsin and 0.4% dispase II (Cat 17105-041) (all from Gibco). The released cells were strained with a 70-μm cell strainer (BD Falcon ...
-
bioRxiv - Cell Biology 2020Quote: ... 300 ng were reverse transcribed using Superscript II reverse transcriptase (LifeTechnologies/Invitrogen) using random hexamers (LifeTechnologies/Invitrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... complementary DNAs (cDNA) were generated using Superscript II Reverse Transcriptase (Life Technologies) according to the manufacturer’s instructions ...