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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Protein levels were quantified using the Pierce BCA Protein Assay Kit (Thermofisher #PI23225) and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). SDS was then added to lysates and incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using the Qubit protein assay kit (ThermoFisher Scientific, Q33211).
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bioRxiv - Neuroscience 2024Quote: ... and VMH protein lysates were determined using a BCA protein assay (Thermo Fisher). Samples were probed with antibodies against UCP1 (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay (Cat# 23235, Thermofisher), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... Proteins were quantified using commercially available Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Neuroscience 2024Quote: ... protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were measured using a BCA Protein Assay Kit (Thermo Fisher Scientific), alongside a BSA protein standard (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... total protein concentration was quantified using BCA Protein assay (Thermo Fisher Scientific, Inc.). A total of 30 µg of proteins in breastmilk samples were separated on 8 or 10% Tris-Glycine gels (Titan Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Protein concentration was determined with the bicinchoninic acid (BCA) protein assay kit (ThermoFisher). Lysate (40-50 µg ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured using the detergent-compatible (BCA) protein assay (Thermo Scientific). Denatured lysates were separated on NuPage 8-16% Tris-glycine Midi Protein gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Thermofisher Scientific) and normalized to 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Bulk proteins were visualized with the No-Stain Protein Labeling Reagent (#A44717, ThermoFisher) and imaged using the iBright Imaging System (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of the lysates were collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of lysate was collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantification of protein concentration was processed using the BCA protein assay (Thermo fisher). A total protein at concentration per lane at 50 μg was loaded into the 10% SDS-PAGE and then transferred to PVDF membranes ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-PCR was performed to obtain cDNA using Superscript III reverse transcriptase and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). QPCR was further performed using Bio-Rad SsoAdvanced universal SYBR green supermix on the Biorad CFX Connect real-time PCR system ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described (Lorin and Mouquet ...
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bioRxiv - Neuroscience 2020Quote: To label purified recombinant tau (monomeric or aggregated tau P301S) with Dylight 488 NHS ester (Thermo Fisher Scientific) or pH-sensitive form of rhodamine (pHrodo ...
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bioRxiv - Immunology 2022Quote: ... The recombinant product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific, MA, USA) to generate the pNDV-HXP-S rescue plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... HMLE.M cells were induced after prolonged treatment with 2.5 ng/ml recombinant TGFβ1 (Life Technologies, Cergy-Pontoise, France). Cell proliferation and survival analyses were performed in 3 or more independent experiments ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Synthetic Biology 2019Quote: ... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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bioRxiv - Immunology 2020Quote: ... Recombinant viruses were generated in Vero E6 cells (ATCC-CRL1586) grown in DMEM high glucose media (Gibco #11995065) supplemented with 10% Hyclone Fetal Clone II (GE #SH3006603HI) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Immunology 2022Quote: ... Recombinant baculoviruses were produced using the Bac-to-Bac baculovirus expression system according to the manufacturer’s manual (Invitrogen).The scheme for constructing the recombinant baculoviruses ...
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bioRxiv - Microbiology 2022Quote: ... Bacmids and recombinant baculoviruses were generated from the pFastBac Dual vectors using the Bac-to-Bac system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: Tissue microarrays were stained using the GPER recombinant rabbit monoclonal antibody clone:20H15L21 (Thermo Fisher Scientific, catalog #703480) by DCL Pathology (Indianapolis ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB10 with DNA isolated from recombinant strains (via the Whole Blood Genomic DNA Purification Kit, Thermo Scientific) followed by selection on LB agar with the appropriate drug ...
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bioRxiv - Immunology 2023Quote: ... was made by mixing 2.5ug of IL-2 monoclonal antibody (JES6-1A12, 2B Scientific) with 7.5ug recombinant IL-2 (ThermoFisher) in 200ul of PBS for 20 minutes at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... and the concentration of the recombinant plasmids was determined using the Qubit dsDNA HS Assay Kit (ThermoFisher, Q32851). 293FT cells were transfected with 1 µg of recombinant plasmids or blank vectors with 40 ng of pRL-TK Renilla luciferase control vector using Lipofiter ...
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bioRxiv - Genomics 2023Quote: ... residual RNA contamination was removed by incubating the samples in 10µL of 1mg/mL recombinant RNase (Ambion, #AM2269). Sample aliquots of 100µL were kept at -80 °C.
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bioRxiv - Neuroscience 2023Quote: ... 0.134% Triton X-100 [Sigma], 0.5 U/µL recombinant RNase inhibitor [Takara, 2313B], 3.33 mM each dNTP [ThermoFisher, R1121] ...
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bioRxiv - Bioengineering 2023Quote: ... The recombinant antibodies were transiently expressed in both Expi CHO and Expi293 cells according to manufacturer’s protocol (Invitrogen). The supernatants from 1mL transient transfections of the antibodies were purified using the AssayMAP BRAVO platform with 5mL Protein A cartridges (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... alanine derivatives were used in a concentration of 10 mM and RNaseOUT recombinant ribonuclease inhibitor (1 µl) (Invitrogen) was added ...
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bioRxiv - Evolutionary Biology 2023Quote: ... secondary antibody (goat anti-mouse IgG H + L superclonal recombinant secondary antibody with Alexa Fluor 488; Invitrogen #A28175) 1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with a mixture of ketamine/xylazine and then infected via retroorbital injection of 100µl containing 1×105 genome equivalents (G.E.) of recombinant NrHV diluted in DPBS (Gibco) (N = 5/strain) ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Genetics 2024Quote: ... The recombinant plasmids were transfected into 293T cells using LipofectamineTM 3000 reagent (Thermo Fisher Scientific, Waltham, MA, USA). For RT-qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant baculoviruses were prepared in the Spodoptera frugiperda (sf9) cells using the Bac-to-Bac system (Life Technologies). HIF2α and BMAL1 were co-expressed in Trichoplusia ni Hive Five cells by infection of 25 ml each of baculoviruses per 1 L of High Five culture ...