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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The antibodies used in immunoprecipitation were Pol II (#39097, Thermo Fisher, USA) and HIF1α (#14179 ...
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bioRxiv - Genetics 2023Quote: ... resuspended and denatured in Gel Loading Buffer II (AM8546G Thermo Fisher Scientific), and run on a 15% TBE/Urea gel ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.5% AlboMAX II Lipid-Rich BSA (gibco, Thermo Fisher Scientific), 0.2 mM hypoxanthine (c.c ...
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bioRxiv - Plant Biology 2023Quote: ... Recombination into destination vectors was achieved using Gateway LR Clonase II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription was performed using the Superscript II reverse transcriptase system (Invitrogen). PCR reactions (Supplementary Fig ...
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bioRxiv - Microbiology 2023Quote: ... enhanced with 5 g/l of Albumax II (Thermo Fisher Scientific, 11021037), 0.12 mM hypoxanthine (prepared by adding 1.2 ml of 0.1M hypoxanthine to 1 M NaOH) ...
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bioRxiv - Biophysics 2023Quote: ... Labtek chambers (Lab-Tek II chambered coverglass system, ThermoFisher Scientific, MA, USA) were passivated with 1 mg/ml BSA (PAA laboratories GmbH ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell number was counted with a Countess II Automated Cell Counter (Invitrogen) on days 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... as confirmed with 0.2% (w/v) Trypan Blue staining (Countess II, Invitrogen). Cells were captured in droplets ...
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bioRxiv - Cell Biology 2023Quote: ... cells were counted using an automated Countess II cell counter (Thermo Fisher).
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bioRxiv - Biochemistry 2023Quote: ... The Sf9 cells were cultured in Sf-900 II SFM (Gibco, USA). Bacmid DNAs were generated and transfected into Sf9 cells to produce and amplify baculovirus ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C in Sf-900 II SFM medium (Gibco) supplemented with 5% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... were grown at 27 °C in Sf-900 II SFM medium (Gibco) supplemented with 5% (v/v ...
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bioRxiv - Genomics 2023Quote: cDNA synthesis was performed following the Superscript II Reverse Transcriptase (ThermoFisher-18064014) (or Maxima H Minus Reverse Transcriptase-ThermoFisher-EP0752 ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription of total RNA was done using Superscript II kit (Invitrogen) and both random hexamers (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... were transfected with 1 μg of bacmid using Cellfectin II reagent (Gibco) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Phusion Green Hot Start II High-Fidelity PCR Master Mix (Thermo Fisher) and primers annealing to the target region (Table S13) ...
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bioRxiv - Plant Biology 2024Quote: ... the entry vector and the Gateway LR Clonase II enzyme mix (Invitrogen) were used to integrate the ABCC4 coding region into Gateway binary vectors pER8-GW-HA (Zuo et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Nuclei were quantified using a Countess II FL automated cell counter (ThermoFisher) and ∼10,000 nuclei were targeted for transposition and capture using a 10X Chromium controller ...
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bioRxiv - Cell Biology 2024Quote: ... using the Gateway™ LR Clonase™ II Enzyme mix (ThermoFisher Scientific). The final vectors sequences were validated by Sanger sequencing (Eurofins Discovery).
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bioRxiv - Cell Biology 2024Quote: ... using the Gateway™ BP Clonase™ II Enzyme mix (ThermoFisher Scientific). The sequences were subsequently cloned in the destination vector MAC-tag-C ...
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bioRxiv - Immunology 2024Quote: ... The ORFs were moved into pTRIP.CMV.IVSb.ires.TagRFP-DEST using LR Clonase II (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using the Superscript II First-Strand Synthesis Kit (Invitrogen). SYBR Green qPCR was performed using a CFX96 thermocycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... coated Nunc Lab-Tek II chamber Slide System (Thermo Fisher Scientific, 154526) 4 hours prior to siRNA transfection as described above ...
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bioRxiv - Cell Biology 2024Quote: ... 1 uL of Platinum SuperFi II DNA Polymerase (12361010, Thermo Fisher Scientific), 10 uL of the 5X SuperFi II Buffer and nuclease free water in a total volume of 50 µL ...
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bioRxiv - Microbiology 2024Quote: ... Purified RNA was reverse-transcribed using SuperScript II (#18064-014, Invitrogen, USA) and the DROAA primer ...
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bioRxiv - Neuroscience 2024Quote: ... pegRNA oligonucleotides were sub-cloned into pCR Blunt II-TOPO backbone (ThermoFisher). A golden gate assembly (GGA ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthetized using SuperScript II and random hexamer (both from Invitrogen). Quantitative gene expression was performed using SYBR Green master mix on a LightCycler 480 Instrument (both from Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... an eGFP open reading frame was transferred from pDONR221- eGFP (Addgene #25899)37 into pInducer20 (Addgene #44012)38 using the Gateway LR Clonase II Enzyme Mix (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified with a Micro BCA protein assay kit (Thermo Scientific, Rockford, IL) using the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The protein concentration was determined using the Bicinchoninic Acid protein assay (Thermo Scientific, US). Lysates (3μg/μl in 1xSDS ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was adjusted using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). For co-immunoprecipitation assay ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins separated by SDS-PAGE were stained with PageBlue Protein Staining Solution (Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentrations were determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), before extracts were stored at −80°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations were determined using a bicinchoninic acid protein assay kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were estimated using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific# 23227). 400-500μg of protein from the stably transfected HEK293 cells were used to immunoprecipitate FLAG-tagged MIB1 using EZview™ Red ANTI-FLAG® M2 affinity gel (MilliporeSigma# F2426 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a Pierce BCA Protein Assay kit (Thermofisher Scientific, 23225) colorimetric reaction and quantified on a SpectraMax i3 Multi-Mode Platform (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... 40uL of a 1:1 mixture of Protein A and Protein G Dynabeads (Invitrogen) were added to each well and then incubated at 4ºC for 4 hours on a rotator ...
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bioRxiv - Immunology 2021Quote: ... Total protein concentration was assessed using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts of whole-cell protein lysates samples were boiled in Laemmli loading buffer ...
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bioRxiv - Genetics 2021Quote: ... Protein amounts were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher). Equal amounts of protein (in vitro samples ...
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bioRxiv - Microbiology 2021Quote: ... Protein concentration was determined via the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted with Tissue Protein extraction reagent (T-PER, Thermofisher Scientific, USA) + Protease inhibitor cocktail (Pierce #A32961) ...
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bioRxiv - Neuroscience 2020Quote: ... Data were normalized to total protein concentration as determined by BCA protein assay (ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: ... protein concentration of lysates was measured using Pierce™ BCA Protein Assay Kit (ThermoFisher), to ensure equal loading of proteins ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts (100 µg of protein) were incubated with streptavidin agarose beads (Thermo Scientific) for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were measured using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). Cytoplasmic and nuclear samples from each brain region containing 5μg total protein was boiled in Laemmli loading buffer and run on a 4%–20% Bio-Rad precast gel ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein content was quantified with a Pierce BCA Protein Assay Kit (Thermo Scientific). The lysate [50 µg protein/50 µL] was incubated with 0.5 µL of 1 M DTT solution at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and the protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher). 50 μg of protein was loaded into each lane of Mini-Protean TGX gels (Biorad ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein amount was confirmed with a BCA protein assay kit (Thermo Fisher Scientific). Proteins were reduced by treatment with 10 mM DTT for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific). Afterwards ...