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Citations for Thermo Fisher :
8501 - 8550 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... WT-PGP1 were labeled with 5 μM CellTracker™ Blue 7-amino-4-chloromethylcoumarin CMAC (Molecular Probes, #C2110), iNeuron-PGP1 was labeled with 2.5 μM CellTracker™ Green CMFDA (Molecular Probes ...
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bioRxiv - Molecular Biology 2019Quote: ... for 5 minutes and triturated 10x in 1ml RT DNase buffer (0.5g/L DNase, 0.35% Glucose, in BME –GIBCO-) to obtain single cell suspension ...
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bioRxiv - Genetics 2019Quote: Ten week-old mice were killed and PMs were collected by peritoneal lavage with 5 mL PBS (Gibco). The cell suspension was filtered on a 100 µm cell strainer and centrifuged at 800g for 10 minutes at 4°C ...
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bioRxiv - Genomics 2021Quote: ... They were cultured at 37°C with 5% CO2 in McCoy’s 5A medium GlutaMAX™ Supplement (Gibco, 36600021) with 10% FBS (Gibco ...
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bioRxiv - Genomics 2021Quote: ... and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems) with SYBR Green detection ...
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bioRxiv - Genetics 2021Quote: ... incubated at 37°C for 5 minutes with Tryple Express dissociation reagent (Fisher Scientific, Cat#: 12-605-010), and collected via scraping ...
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bioRxiv - Cell Biology 2021Quote: HEK293T and SH-SY5Y cell lines were maintained at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Biochrom KG) supplemented with 10% fetal bovine serum (Gibco), 100 U ml−1 penicillin (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... the TSCs were plated on 5 µg/ml Collagen IV (corning)-coated dishes with TSCM (DMEM/F12 (Gibco) medium supplemented with 1% ITS-X supplement (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... pDONR221-mBCAR3 was moved into pDEST-5’-3xFlag-pcDNA FRT/TO with a LR reaction (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 minutes prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Physiology 2021Quote: ... at a concentration of 1:500 for 5 min and mounted in SlowFade Diamond Antifade Mountant (ThermoFisher, S36967). Images were captured using a Leica TCS SP5 Confocal Microscope and processed using Fiji (ImageJ ...
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bioRxiv - Cell Biology 2021Quote: ... under standard cell culture conditions (37°C, 5% CO) in Dulbecco’s modified Eagle’s medium (DMEM) high glucose medium (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Physiology 2020Quote: ... 600 nM of HIV gag reverse primer and 5 U of Platinum Taq DNA polymerase (Life Technologies; USA). In the 2nd round PCR 5’ LTR region of HIV-1 was amplified using 1 μl of 1st round PCR amplicon as a template ...
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bioRxiv - Molecular Biology 2021Quote: ... and HeLa (ATCC CCL-2) cells were cultured at 37 °C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... and African green monkey kidney epithelial Vero E6 cells (ATCC) were maintained at 37⍰°C with 5% CO2 in high-glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: The immortalized hippocampal neuron HT22 was cultivated at 37 °C in a humidified 5% CO2 atmosphere and maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) with additions of 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... for microscopy slides or 0.8 mg/ml PEG-Silane and 0.2 mg/ml Biotin-PEG-Silane (5 K, Creative PEGWorks) for coverslips and 0.05 mg/ml NeutrAvidin (Invitrogen) in G-Buffer (2 mM Tris-Cl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: HEK293A and HEK293FT cells were cultured at 37 °C in 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% FBS and 100 U/mL penicillin and 100 μg/mL streptomycin ...
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bioRxiv - Biochemistry 2021Quote: ... by heating at 95 °C for 5 min with 2 µL of SDS-PAGE loading dye (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Then pH standard buffers (pH ~6.5 and ~7.5 (0.025 M HEPES, 0.105 M KCl, 0.001 M MgCl2)) were prepared with 10 μM nigericin (Invitrogen, N1495) and added to cells to equilibrate pHe and pHi as previously described.28 For each cell ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... using a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 × 0.32 mm, 5 μm, Thermo Fisher Scientific), installed in a Dionex Ultimate U3000 HPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... and mouse anti-UBE2J1 (1:250) diluted in blocking solution with 0.1% saponin and 5% goat serum (Gibco) for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... filter sterilized using a 5 mL syringe (309646, BD Vacutainer Labware Medical, Fisher Scientific, Hanover Park, IL, USA) with 0.2 µm filter (431219 ...
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bioRxiv - Neuroscience 2021Quote: ... was dissected from embryonic days 13.5 CD1 mouse embryo (50 ganglia per embryo) and incubated with 0.05% trypsin solution containing 0.02% EDTA (Gibco) at 37°C for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... were cultured at 30 °C in the presence of 5% CO2 in medium composed of high glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 5% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: HCT116 MTAP -/- were cultured at 37°C in 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Life Technologies), supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... 5 µl of equal volume supernatants were loaded onto Novex 10% gelatin gels (Life Technologies, Grand Island, NY). Zymography assays were then performed per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and the cells were stimulated with 5 μM A23187 in 400 μL FreeStyle 293 Expression medium (Thermo Fisher) per well ...
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bioRxiv - Neuroscience 2021Quote: ... 5×105 cells were reseeded into T25 flask and transfected using TurboFect (Thermo Fisher Scientific, Waltham, MA, USA) and 4 μg plasmid DNA per flask of the respective construct in pCDNA3.1(+ ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were then rinsed again 5 times with PBS and a 300 nM DAPI solution (Thermo Fisher) was added for 10 minutes.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The qPCR was performed using a QuantStudio 5 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Each sample was run in technical triplicate and results are reported in log bsed format (16S rRNA gene copies/mL).
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bioRxiv - Molecular Biology 2021Quote: ... 5 μm sections were cut from each paraffin block using a microtome (Shandon AS325, ThermoFisher Scientific, Waltham, USA). The resultant sections were haemoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary gastric adenocarcinoma (AGS) cells were maintained at 37°C with 5% CO2 in RPMI-1640 media (Gibco). All media was supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 × 105 per wells were seeded in each well of a six-well culture plate (Thermo Fisher Scientific) and kept overnight ...
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bioRxiv - Cell Biology 2021Quote: ... the block was loaded into the FIB-SEM (Helios 5 UX DualBeam, Thermo Fisher Scientific, Brno, Czech Republic), and the identified regions of interest were located by secondary electron imaging at 5 KeV and 800 pA ...
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bioRxiv - Cell Biology 2020Quote: ... 2 500 HEK293T cells in 50 µl culture medium were reverse transfected using 5 µl of 0.2 µM (Dharmacon) or 0.05 µM (Ambion) siRNA and 0.05 µl RNAiMAX in 10 µl serum-free RPMI 1640 medium per well ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 37°C (5% CO2) in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies), supplemented with 10% fetal bovine serum (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: Raji cells (ATCC) were cultivated at 37°C and 5% CO2 in RPMI 1640 (Gibco, Thermo Fisher, USA) supplemented with 8% FCS (Lot BS225160.5 ...
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bioRxiv - Cell Biology 2019Quote: ... was neutralized with reconstitution buffer (2.2% NaHCO3, 0.05 N NaOH, and 200 mM HEPES) and diluted with 5× DMEM (Gibco). MDCK cells (1.5 × 105 cells/ml of collagen gel ...
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bioRxiv - Cell Biology 2020Quote: ... Cells used in this study were within 20 passages after thawing and were cultured (37°C, 5% CO2) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: Human U-2 OS cells and U-2 OS cells expressing 53BP1-mScarlet from the endogenous promoter25 were grown under standard cell culture conditions (humidified atmosphere, 5% CO2) in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (GIBCO) and penicillin-streptomycin antibiotics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N; Thermo Fisher Scientific). When seeding the cells ...
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bioRxiv - Genetics 2021Quote: RNAseq: 5 μg of total Trizol-purified RNA purified was treated with Turbo DNase free kit (Thermo Fisher), ribosomal depleted using Ribo-Zero Kit (Epicentre ...
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bioRxiv - Genomics 2020Quote: ... Skin samples were washed in hank’s balanced salt Solution (HBSS) supplemented with 5% fetal bovine serum (FBS, Gibco), 2% antibiotic and antimycotic solution (AA ...
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bioRxiv - Microbiology 2020Quote: ... 5 μL of ZIKV RNA was used in a cDNA synthesis reaction with a SuperScript IV kit (Invitrogen) in addition to 6 μL of nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C with 5% CO2 with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies).
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bioRxiv - Evolutionary Biology 2021Quote: ... and a final extension at 72 C for 5 min (Phusion High-Fidelity PCR Master Mix, Thermo Scientific). A final library clean-up step was performed before sequencing (QiAquick PCR purification kit ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies used for IF staining were anti-Pax7 (DSHB, cat# PAX7, RRID:AB_528428; 1/5 dilution; or Invitrogen, cat# PA1-117 ...
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bioRxiv - Molecular Biology 2021Quote: ... Then embryos were washed three times PBTX and blocked with PBTX containing 5% normal goat serum (NGS, Invitrogen) for 1 hour.
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bioRxiv - Molecular Biology 2021Quote: The purified immunocomplexes were boiled during 5 min at 96°C in NuPAGE 4X loading buffer (Life Technologies) and NuPAGE™ Sample Reducing Agent (Life Technologies) ...