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Citations for Thermo Fisher :
8351 - 8400 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated at a final concentration of 5 μM CellROX® Orange reagent (Life Technologies, Carlsbad, USA) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... COS-7 cells (ATCC) were grown at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (Gibco) containing 10% fetal bovine serum and 1% penicillin/streptomycin (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... slides were washed 3 times for 5 min with TNT and mounted with Prolong Gold (P36930, Life Technologies).
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bioRxiv - Cell Biology 2022Quote: U2OS osteosarcoma cells were maintained at 37°C under 5% CO2 and propagated using Dulbeco’s modified Eagle’s medium (DMEM) (no. 11965-084, Gibco) supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... the IP sample was spun down at 13,000 r.p.m for 5 min and the supernatant was added to 60 μl of Dynabeads protein G (Invitrogen), previously equilibrated with IP buffer ...
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bioRxiv - Immunology 2022Quote: Two groups of PBMCs (5×106 cells/ml) were stimulated with 1μg/ml anti-CD3/CD28 antibodies (Invitrogen) at 37°C under 5% CO2 for 18 hours in RPMI-1640 medium ...
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bioRxiv - Immunology 2022Quote: ... primed cells were washed and then activated with 5 mM ATP diluted in Opti-MEM media (Thermo Fisher) for 60 min and supernatants collected.
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bioRxiv - Molecular Biology 2022Quote: Cos7 cells were cultivated at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM (Gibco/ ThermoScientific)) with 1% pyruvate ...
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bioRxiv - Neuroscience 2022Quote: ... The heads were then homogenized in loading buffer (4X Bolt LDS, Invitrogen, Novex with 5% β-mercaptoethanol added) and heated for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... OT-I and OT-II cells were labeled with 5 μM of carboxyfluorescein succinimidyl ester (CFSE, ThermoFisher Scientific). Ag-loaded APCs were cultured with equivalent number of OT-I cells ...
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bioRxiv - Biochemistry 2022Quote: ... All cell lines were cultivated at 37°C and 5% CO2 in a humidified incubator using DMEM (ThermoFisher) + 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... 5 μl of 7-amino-actinomycin D (7AAD, 50 μg/ml working solution in PBS, Thermo Fisher Scientific) was added to the stained cells 5–10 min before measurement.
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bioRxiv - Immunology 2022Quote: ... mice were fasted overnight and then gavaged with 100 μl of rhodamine B-dextran (5 mg/ml; ThermoFisher) in 2% methylcellulose ...
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bioRxiv - Plant Biology 2022Quote: ... an additional cleaning step with acid phenol was added before precipitation of the RNA by adding an equal volume of Acid-Phenol:Chloroform 5:1 solution pH 4.5 (Ambion). Total RNA was quantified with a Nanodrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged at 10,000 ×g for 5 min with a benchtop centrifuge (Thermo Fisher Scientific). After removing the supernatant ...
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bioRxiv - Molecular Biology 2022Quote: ... HCT116 were cultured at 37 °C in a 5% CO2 humidified incubator in McCoy’s 5A (Modified) Medium (ThermoFisher) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... to stain AAV2/5-GfaABC1D::eGFP and Alexa 488 anti-chicken (1:400, Thermo Fisher Scientific, The Netherlands). Finally ...
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bioRxiv - Microbiology 2022Quote: BHK-21 cells were maintained at 37°C with 5% CO2 in Dulbecco modified Eagle medium (DMEM, Gibco) plus 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... according to the manufacturer’s instructions in optical plates on a QuantStudio 5 Real-Time PCR system (ThermoFisher Scientific) equipped with 384-well block ...
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bioRxiv - Microbiology 2022Quote: 5 μL of RNA from infected cell monolayers was reverse transcribed using random hexamers and Superscript III (ThermoFisher) to generate the first cDNA strand for each sample according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... We then switched the medium from LB to LB containing the antibiotic colistin (5 μg/mL, Acros organics) and the dye Propidium Iodide (5 μM ...
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 2 hours with a solution containing 0.5% TritonX-100 and 5% donkey serum (Thermo Fisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Cell Biology 2022Quote: HCT 116 cells were cultured at 37°C and 5 % CO2 in FluoroBrite DMEM (Cat# A1896701, DMEM, Gibco) with 4.5 g/L glucose ...
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bioRxiv - Microbiology 2022Quote: ... infected Jurkat cells were initially preloaded with 5 µM of CellTracker CMAC (7-amino-4-chloromethylcoumarin) (Life Technologies) and cocultured for 6 or 24 h with MDMs plated onto coverslips ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μl of the dsDNA was used to quantify the DNA concentration with the Qubit assay (Invitrogen, Q32853) according to the manufacture’s protocol.
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bioRxiv - Microbiology 2022Quote: ... The reactions were then run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA).
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... QPCR was done using RealQ Plus 2x Master Mix Green (Ampliqon, #A324402) on a QuantStudio 5 (Applied Biosystems) machine ...
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bioRxiv - Bioengineering 2022Quote: ... and cultured at 37°C in a humidified incubator with 5% CO2 in complete growth media consisting of Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely cultured at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM} supplemented with 10% FBS (Gibco), 100 U/mL penicillin and 100 μg/mL streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured for 5-7 days at P0 before been passaged with Tryspin - EDTA 0.05% (Thermo Scientific, 25300054). Cells were passaged every 4-5 days and until p8 ...
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bioRxiv - Developmental Biology 2022Quote: ... The sliced tissue was then transferred to a glass tube along with a magnetic stir bar and 1.5 ml digestion buffer in Dulbecco’s modified Eagle’s medium containing collagenase type II (250 U/ml) (17101015; Gibco), collagenase type IV (300 U/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL were injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (Thermo Scientific,Waltham MA, USA). Peptides were loaded onto a μ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37 °C and 5% CO2 in EMEM (ATCC) supplemented with 0.01 mg/mL human recombinant insulin (Gibco) and 10% (vol/vol ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were spun down at 500 g for 5 min washed with phosphate-buffered saline (PBS; Thermo Fisher, catalog no ...
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bioRxiv - Microbiology 2020Quote: Cells grown until A600 of ∼0.1 were labelled with 10 μM EdU (5-Ethynyl-2’-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed ...
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bioRxiv - Neuroscience 2019Quote: ... The right sciatic nerve was exposed and injected with 5 μl of WGA alexa-fluor-488 (Invitrogen, USA), using a 10-μl microsyringe ...
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bioRxiv - Microbiology 2021Quote: ... as the basal carbon source and were supplemented with 5% FBS and different concentrations of glucose (11.1mM, 22.2mM and 44.4mM) (Gibco, USA) and keeping basal glucose concentration at 11.1mM ...
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In vivo imaging of the kinetics of microglial self-renewal and maturation in the adult visual cortexbioRxiv - Neuroscience 2020Quote: ... clone G-A-5) followed by a secondary antibody solution (4 h, RT, AlexaFluor 594, 1:500, Invitrogen), mounted and coverslipped ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue homogenates were then cleared by centrifugation twice (10.000g, 10min and 10.000g, 5 mins) and assayed for protein concentration using the BSA kit from ThermoFisher.
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bioRxiv - Plant Biology 2021Quote: ... Proteins in the clear supernatant (protein extract) were cross-linked with 5 mM DSP (Thermo Fisher Scientific Pierce) for 2 h with agitation ...
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bioRxiv - Immunology 2021Quote: ... cells were dumped and washed off the plate with 1x PBS and blocked with 5% FBS (Life Technologies) in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T cells were cultured at 37°C in a humidified 5% CO2 atmosphere in DMEM (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum and penicillin/streptomycin.
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bioRxiv - Neuroscience 2021Quote: ... the washing medium was removed and 80 L of 5 M cell permeant Fluo-4 AM (Life technologies) in neurobasal medium was added to wells at room temperature and incubated at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... and grown at 37°C in 5% CO2 in growth medium (GM): high-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... at 37°C with 5% CO2 with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies).
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... WT cells were stained directly in the 24 well plate with 5 ng/ml FM4-64 (Thermo Fisher) dye ...
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bioRxiv - Microbiology 2020Quote: ... 1976) and maintained in 6% O2 and 5% CO2 and grown in sterile filtered RPMI 1640 media (Gibco) supplemented with 2 M HEPES ...