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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Cells were cultured at 37 °C and 5 % CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Thermo Fisher Scientific) containing 4.5 g l−1 D-glucose and L-glutamine supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... were grown at 37°C in 5% CO2 with 1% Penicillin/Streptomycin (PS; 5000U.mL-1 and 5000µg.mL-1; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... the proliferation capacity of NK92 cells was measured by an EdU (5-ethynyl-2 ‘-deoxyuridine, Invitrogen, CA, USA) proliferation assay ...
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bioRxiv - Developmental Biology 2020Quote: ... Approximately 5 μg of the digested total RNA was reverse-transcribed using the M-MLV reverse transcriptase (Invitrogen) and random primers (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... 1:200 in 1% PBSA and then incubated for 5 min with Syto9 (1:1000) ® (Thermo Scientific) for nuclear staining ...
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bioRxiv - Immunology 2020Quote: ... mice were injected in the tail vein with 0.1 ml of 5% dextran (3,000 kDa Texas Red conjugated, Invitrogen) diluted in PBS 10 min prior to blood feeding ...
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bioRxiv - Microbiology 2021Quote: ... CD4(+) T cells were stimulated with 5 μg/mL phytohemagglutinin (PHA; MiliporeSigma) in complete RPMI-1640 (Thermo Fisher) supplemented with 10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... 5% CO2 for a further 72 hours following which the cells were stained with NucBlue dye (Invitrogen, #R37605) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: THP-1 cells (ATCC, TIB-202) were cultured at 37°C with 5% CO2 in RPMI 1640 (Invitrogen) supplemented with 10% heat inactivated FBS (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: NK cells were cultured in CTS AIM V SFM containing 5% CTS Immune cell SR (ThermoFisher, Waltham, MA), Penicillin/Streptomycin ...
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bioRxiv - Biophysics 2021Quote: ... Cells were incubated with 10 μM of the synthetic nucleoside 5-Ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific) for 25 min at 37°C and 5% CO2.
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bioRxiv - Bioengineering 2020Quote: ... hESCs and iPSCs were single-cell dissociated by 5 min incubation in EDTA (0.5 mM, Thermo Fisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... DNA was stained with Sytox green nucleic acid stain (5 mM solution in DMSO from Thermo Fisher Scientific) and analyzed on a BD Accuri flow cytometer.
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell suspension was obtained by detaching preadipocytes with 5 mL of 0.25% trypsin (ThermoFisher Scientific Inc./Gibco) for 3 min at 37C ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell suspension was obtained by detaching preadipocytes with 5 mL of 0.25% trypsin (ThermoFisher Scientific Inc./Gibco) for 3 min at 37C ...
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bioRxiv - Genetics 2022Quote: ... can be measured in live cells by using 5-(pentafluorobenzoylamino) fluorescein Di-β-D-glucopyranoside (PFB-FDGlu; ThermoFisher) as a substrate that is taken up into acidic vesicles only ...
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bioRxiv - Microbiology 2022Quote: ... vaginalis prelabeled with 5 μM of carboxyfluorescein diacetate succinimidyl ester dye (CFSE; CellTraceTM, Thermo Fisher Scientific, Massachusetts, USA), were inoculated by a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... and 1.2 ul TMPRSS2/pLX304 or ACE2/pLJM1 (1.64 pmol) in 5 ml OptiMEM medium (Thermo Scientific 31985062) mixed by pipetting and incubated for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: Intracellular ROS production was detected by staining cells with 5 μM MitoSOX Red (Life Technologies, Frederick, Maryland, USA) in DMSO ...
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bioRxiv - Systems Biology 2022Quote: ... or ‘cellROX orange’ to stain intracellular ROS (at 5 µM final concentration, Thermo Fisher scientific Cat. No. C10443). Finally ...
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bioRxiv - Immunology 2022Quote: ... Plates were developed for 5-15 min at RT using 100µl of TMB (Invitrogen Ref. 50-112-9758) and the reaction stopped with H2SO4 ...
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bioRxiv - Genetics 2022Quote: ... A second DNase treatment was performed on the purified RNA using 5 μL of Turbo DNase (Life Technologies) in 300 μL of total volume for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: HeLaM-iORF7a cells were incubated in cysteine- and methionine-free Dulbecco’s modified Eagle’s medium supplemented with 5% dialyzed fetal bovine serum (Invitrogen), 1% Glutamax ...
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bioRxiv - Cell Biology 2022Quote: ... The MSCs used in the confocal imaging study were also stained with 5 mg/L Hoechst 33342 (Thermofisher) in the same 50 mL conical tube ...
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bioRxiv - Cell Biology 2022Quote: ... the chemiluminescent signal was elicited by 5 minute incubation with enhanced chemiluminescence substrate (ECL, Pierce, Thermo Fisher Scientific) and captured photographically on film (CL-XPosure ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections of 5 μm were stained for H&E and mounted with Permount Mounting Medium (Thermo Fisher Scientific). For immunohistochemistry ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5 μg of RNA was reverse transcribed using Maxima cDNA Synthesis Kit following manufacturer’s protocol (Fisher Scientific). The resulting cDNA was submitted to PCR analysis using PCRBIO VeriFi™ Mix (PCRBIOSYSTEMS ...
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bioRxiv - Cell Biology 2022Quote: ... and media was replaced with 5 mL complete mTeSR media supplemented with 1X ReVitaCell ROCK Inhibitor (Thermo Fisher). Cells were gently ...
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bioRxiv - Microbiology 2022Quote: ... Between 1 and 5 μg of RNA was boiled at 95 °C with Gel II Loading Buffer (Invitrogen) and loaded on a urea-PAGE gel (Sequagel ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The plates were run on a 384-well QuantStudio™ 5 Real-Time PCR System (Applied Biosystems A28575) using the pre-set comparative-CT-Sybr with melt curve program with the reaction volume adjusted to 10 μL ...
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bioRxiv - Microbiology 2022Quote: For the neutralization assays Vero E6 cells were cultured at 37°C and 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% (vol/vol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections of 5 μm-thick were cut with a microtome before mounted on Superfrost-Plus slides (ThermoFIsher Sci.). Haematoxylin and eosin staining was performed using a Leica Autostainer XL (Leica Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... All cell lines were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, MA, USA) with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Procyclic forms were cultured at 27°C in MEM-Pros medium [21] supplemented with 5% Penicillin/Streptomycin (Gibco), 7.5 mg/l hemin and 10% of heat inactivated fetal bovine serum at a density between 105 - 4×106 cells/ml.
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Immunology 2019Quote: ... An eight-point dilution series of patient serum diluted at 1:5 in DMEM (Gibco, Life Technologies, USA) supplemented with 2% FBS were incubated with a constant amount of DENV1 - West Pac 74 and DENV-2 S16803 ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesized from 5 µg RNA with 0.5 µg of oligo(dT)12-18 primer (Thermo Fisher) using SuperScript III reverse transcriptase (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... miR-125b+A or control RNA 3’O-methyl-modified mimic using 5 μl Lipofectamine 2000 (Life Technologies) in a 500 μl reaction mixture according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... T47D:A18 cells and T47D:A18-4HT16 cells were grown in 5% fetal bovine serum (FBS) in RPMI (Life Technologies). T47D:C4217 cells were grown in 5% charcoal stripped calf serum in phenol red free modified RPMI media (Life Technologies) ...
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bioRxiv - Biochemistry 2019Quote: ... 5 mM MgCl2) with 1.5% n-Dodecyl β-D-maltoside (DDM) and 1X Halt Protease Inhibitor (Thermo Scientific) for 30 minutes on ice ...
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bioRxiv - Cell Biology 2019Quote: ... at 4% haematocrit in supplemented RPMI media (RPMI-HEPES, 0.2% NaHC03, 5% heat-inactivated human serum [Australian Red Cross], 0.25% AlbumaxII [GIBCO] ...
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bioRxiv - Synthetic Biology 2019Quote: ... incubated for 5 minutes at RT and transferred to the DynaMag-96 Side Magnet (#12331D, Thermo Fisher Scientific). Following incubation of the sample on the magnet for 2 minutes (or until sample is clear) ...
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bioRxiv - Cell Biology 2019Quote: ... and III (DE) (1.7 kb) of the Cyclin E gene 5’ flanking sequence were synthesized by GeneArt (Invitrogen) with appropriate restriction sites at both ends and subcloned into the pMA plasmid to generate pMA-P ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from fly heads was isolated from 5-day-old female flies with TRIzol reagent (Invitrogen, USA). Reverse transcription of RNA to cDNA was achieved using 2 μg of total RNA with random hexamer primers using RT-PCR SuperMix Kit (TransGen Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then treated with ExoSAP-IT enzyme (2 μl of enzyme for 5 μl of sample, ThermoFisher) at 37 °C for 15 min followed by a 15 min inactivation at 80 °C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and 2 pmol of each primer (5’ primers labeled with Applied Biosystems fluorescent dyes FAM, HEX or NED). Thermal cycling (under mineral oil ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
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bioRxiv - Neuroscience 2019Quote: ... rinsed again (3 × 5 min) in 1X PBS and mounted using SlowFade™ Diamond Antifade Mountant (Invitrogen; S36963). Fluorescence images were acquired with a confocal laser scanning microscope (LSM 700 or 710 ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Microbiology 2019Quote: ... PUUV-Gc-rev 5’-ATTCCCGGGCTTGTGCTCCTTC-3’ to PCR amplify (Phusion Flash High-Fidelity PCR Master Mix, ThermoFisher Scientific) the inserts from codon optimized PUUV GPC described in Iheozor-Ejiofor et al. ...