Labshake search
Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
-
bioRxiv - Genomics 2021Quote: ... Human K562 erythroleukemia cells were cultured in Roswell Park Memorial Institute (RPMI, Life Technologies) 1640 medium supplemented with 10% FBS ...
-
bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated from human desmoid tumor cell lines using Trizol reagent (Invitrogen) according to the manufacturer instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... Primed human ESC and iPSC cells were harvested with 0.25% trypsin-EDTA solution (Invitrogen), and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System ...
-
bioRxiv - Microbiology 2022Quote: ... human foreskin fibroblast (HFF) monolayers were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 25 mM glucose (Sigma-Aldrich) ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
-
bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... except for human XBP1S that was assessed using SYBR green master mix (Thermofisher Scientific) using primers in Supplementary Table 3.
-
bioRxiv - Genomics 2020Quote: The THP1 (human acute monocytic leukemia derived; ATCC number TIB-202 purchased from Thermofisher) cell line[18] was regularly maintained in RPMI 1640 medium containing GlutaMAX™ ...
-
bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
-
bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA ...
-
bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
-
bioRxiv - Immunology 2021Quote: ... coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher, #H17000) in 0.1 M carbonate buffer ...
-
bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific) and cultured in DMEM medium (Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: Human GCDH cDNA (clone ID 3142978) in pOTB7 vector was obtained from Thermo Fisher Scientific Open Biosystems repository (now part of Horizon Discovery) ...
-
bioRxiv - Biochemistry 2020Quote: ... 7.5 μg/mL human IgG and 50 μg/mL BSA-biotin (Thermo Fisher Scientific) were also printed to serve as a printing control and “intra-well signal normalizer” ...
-
bioRxiv - Biochemistry 2020Quote: ... and negative selection using the Dynabeads Untouched Human CD4 T Cells kit (Invitrogen 11346D) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... Gibco™ FGF-Basic AA 1-155 recombinant human protein (bFGF, PHG0264, Fisher Scientific), endothelial cell basal medium 2 (EGM 2 ...
-
bioRxiv - Biophysics 2021Quote: ... CD4+ T cells were stimulated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher Scientific) and cultured in AIM V Medium (ThermoFisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: Human embryonic kidney293 (HEK293) cells were maintained in Dulbecco’s Modified Eagle’s Medium (Life Technologies) supplemented with 10% fetal bovine serum (Atlanta biologicals ...
-
bioRxiv - Biophysics 2021Quote: ... The human Gβ1 and Gγ2 subunits were integrated into the pFastBac Dual vector (Invitrogen) with an N-terminal 6 × His-tag at the N terminus of Gβ1 ...
-
bioRxiv - Cancer Biology 2020Quote: ... T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher, Waltham, MA) at a 2:1 bead:cell ratio for 48 h prior to electroporation ...
-
bioRxiv - Cell Biology 2021Quote: Undifferentiated human neuroblastoma SH-SY5Y cells were cultured in DMEM/F12 medium (Gibco, 11320033) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: ... Rb polyclonal antibody anti-GMAP-210 (proteintech) or Ms monoclonal anti-Trip230 human (Invitrogen). Images were acquired on a Leica confocal TCS SP8 STED microscope ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1ẞ high sensitivity kit sold by Invitrogen. The sample for our ELISA consisted of cell culture media (referred to as sample hereafter ...
-
bioRxiv - Bioengineering 2021Quote: ... 100 μL of 5 μg/mL fibrinogen from human plasma conjugated to AF488 (Invitrogen) was added to each well ...
-
bioRxiv - Neuroscience 2020Quote: ... The following primary antibodies were used: Mouse anti-human synuclein (1:5000, Thermo Fisher Scientific ...
-
bioRxiv - Systems Biology 2020Quote: ... anti-human CD20 mouse IgG2 monoclonal antibody clone 2H7 (Invitrogen, cat: 14-0209-82), anti-human CD38 chimeric monoclonal antibody kindly provided by Centrose LLC ...
-
bioRxiv - Microbiology 2021Quote: ... Goat anti-human and anti-mouse antibodies pre-coupled to Alexa Fluor 647 (Invitrogen) were used as secondary antibodies in flow cytometry experiments ...
-
bioRxiv - Microbiology 2021Quote: ... A mating strategy was used to screen a commercial human spleen cDNA library (Invitrogen) established in the pPC86 vector to express cellular proteins in fusion downstream of the GAL4 transactivation domain (GAL4-AD) ...
-
bioRxiv - Microbiology 2021Quote: ... Human DDX42 cDNA was amplified by RT-PCR using the SuperScript III™ (Invitrogen) from mRNAs of MDMs using primers DDX42-forward 5’-AATTAATTTAGGATCCATGAACTGGAATAAAGGTGGTCCTG and DDX42-reverse 5’-AATTAATTTACTCGAGCTAACTGTCCCATCGACTTTTCTTGCG ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNA from mouse and human atrial tissue was extracted using Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... The Taqman probes used for human and mouse genes were purchased from Applied Biosystems and listed in Table S5.
-
bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...
-
bioRxiv - Cancer Biology 2020Quote: RNA was extracted from human breast tissue after homogenization into TRIzol (Ambion, Life Technologies) using Qiagen Tissue Lyser II with metal beads ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary human lung fibroblasts were maintained in DMEM (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Sequencing libraries were generated using Ion AmpliSeq Transcriptome Human Gene Expression Panel (ThermoFisher Scientific). Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... FITC conjugated F(ab’)2 goat anti-human IgA (Thermo Fisher Scientific, Illkirch, France), Brilliant Violet 650 conjugated streptavidin (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... Human and mouse IL-1β secretion was quantified by ELISA kits (Thermo Fisher Scientific) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... followed by coating with antibodies: anti-human CD3 (clone OKT3, 10 μg/mL, Invitrogen) and anti-human CD28 (clone CD28.2 ...
-
bioRxiv - Cell Biology 2022Quote: RNAs were collected from the human Sertoli cells using TRIzol (Life Technologies, Waltham, MA) and cDNA was synthesized ...
-
bioRxiv - Cancer Biology 2022Quote: ... T cells were mixed with Human T-activator CD3/CD28 DynaBeads (Thermo Fisher Scientific) in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... was performed using the cDNA of human GPRC6A cloned in the vector pcDNA3 (Invitrogen) as template ...
-
bioRxiv - Bioengineering 2021Quote: ... anti-CD9 (1:500, mouse monoclonal anti-human CD9, clone MM2/57, Invitrogen, AHS0902). The membranes were then washed three times with 0.1% Tween 20 in PBS for 5 minutes at RT ...
-
bioRxiv - Microbiology 2022Quote: ... The goat anti-human IgG conjugated with Alexafluor-647 was purchased from Invitrogen (A21445).
-
bioRxiv - Cancer Biology 2022Quote: Human primary fibroblasts FIB-1 and FIB-2 were cultured in DMEM medium (Gibco) supplemented with 10% FBS and Pen-Strep antibiotics ...
-
bioRxiv - Immunology 2022Quote: ... the following primary antibodies were used: CD69 mouse anti-human (#MA5-15612 Thermo Fisher), IFN-alpha rabbit polyclonal (#PA5-115430 ThermoFisher) ...