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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 462611_mat for mice and assay ID 002275 Cat# 4427975 for human samples (Applied Biosystems) and normalized to U6 expression (Assay ID 001973 ...
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bioRxiv - Biochemistry 2024Quote: ... HeLa cell lysate from the 1-Step Human IVT Kit (Thermo Fisher Scientific #88882) and yeast tRNA (Thermo Fisher Scientific #AM7119 ...
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bioRxiv - Genetics 2024Quote: ... Pooled Invitrogen™ LentiArray™ Human gRNA-PuroR CRISPR-KO Library gRNAs (ThermoFisher #A31949) (MOI 0.3-0.5 ...
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bioRxiv - Biophysics 2024Quote: The wild-type human CCR1 cDNA was cloned into a modified pFastBac1 vector (Invitrogen), incorporating an N-terminal FLAG tag and a C-terminal 8×His-tag for affinity purification ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were activated using Human T-Activator CD3/CD28 DynabeadsTM (Thermo Fisher Scientific) at a bead-to-cell ratio of 1:1 for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 488 Mouse Anti-Human CD274 antibody (Cat# 53-5983-42, Invitrogen)) by Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: Human CTLs were stained for 30 min with Lysotracker red DND-99 (2µM) (Invitrogen) at 37°C/5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... the medium was switched to hECSR1 [Human Endothelial SFM (Thermo Fisher Scientific, Cat.# 11111044) supplemented with 20 ng/mL bFGF (R&D Systems ...
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bioRxiv - Bioengineering 2024Quote: ... the human macrophages were incubated with 10 mM ethylenediaminetetraacetic acid (EDTA, 15575020, Thermo Fisher) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Antibodies used for immunofluorescence included mouse anti-human collagen (1:500; PA5-95137, Invitrogen), mouse anti-human FN (1:1000 ...
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bioRxiv - Bioengineering 2024Quote: ... Human podocytes were cultured in RPMI Medium 1640 (Gibco, ThermoFisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit 594 and anti-human 647 IgG (H+L; Invitrogen, 1:200 dilution), and DAPI (1:500 ...
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bioRxiv - Bioengineering 2024Quote: ... human embryonic kidney HEK293T cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) complemented with fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from human LSC and HSC populations using TRIzol Reagent (Invitrogen) and cleaned-up using RNeasy column (Qiagen) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ng/mL human Fibroblast Growth Factor-2 (FGF-2) (Thermo Fisher, #100-18B), and 1% antibiotic-antimycotic solution (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from human and bovine myotubes using TRIzol reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Human Embryonic Kidney 293T cells (ATCC CRL-3216) were maintained in DMEM (Gibco 11995065) with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Human PAECs were transfected at about 70% confluency using OptiMEM media (Thermo Fisher Scientific) and Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Human iPSCs were cultured in chemically defined conditions in Essential 8 medium (Life Technologies) and grown on Geltrex™ LDEV-Free ...
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bioRxiv - Cell Biology 2020Quote: ... DMEM High-glucose [Gibco] without Riboflavin and Phenolred containing 10% [w/w] FCS [Gibco], 1% [w/w] P/S [Gibco] and 1 % [w/w] Glutamax [Gibco]) supplemented with 1 μM TO-PRO®-3 (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES, Life Technologies, and 2% FCS, Life Technologies) for 30 min ...
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bioRxiv - Pathology 2022Quote: Cells isolated as described in “Erythrocytic Compartment Preparation” and “Tissue Leukocyte Isolation” were incubated with Fc block (1:100) and LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (ThermoFisher Scientific, #L34959; 1:5000 in PBS)(10min. ...
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bioRxiv - Microbiology 2020Quote: ... which were maintained in Minimal Essential Medium (MEM) Rega-3 (Thermo Fisher Scientific) + 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... with single-cell passaging every 2-3 days using accutase (ThermoFisher Scientific A1110501) at split ratios between 1:5 and 1:12.
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were washed 3 times in PBS and secondary antibodies (Oregon Green - Thermofisher O-6382 ...
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bioRxiv - Immunology 2021Quote: ... qPCR was performed on the QuantStudio 3 Real-Time PCR System (Applied Biosystems) using SYBR Green ...
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bioRxiv - Immunology 2021Quote: ... on the QuantStudio 5 or QuantStudio 3 Real-Time PCR System (ThermoFisher Scientific). Relative transcript levels were normalized to TATA-binding protein (Tbp ...
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bioRxiv - Plant Biology 2020Quote: ... from 2-3 weeks old seedlings and 500ng was treated with DNaseI (Ambion) and used for reverse transcription ...
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bioRxiv - Genetics 2020Quote: ... to add 3’A overhangs prior to TOPO TA-cloning (K4600-01, Invitrogen). We then performed colony PCR using the same PCR primers and conditions used to amplify the hmx1 sequence from cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s instructions and run on a Quant Studio 3 System (ThermoFisher) using the default settings for SYBR Green reagents and the fast run mode in the QuantStudio Design & Analysis Software v.1.4.2 ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were performed on a QuantStudio 3 Real-Time PCR System (Thermo Fisher) with the amplification conditions as previously described (14).
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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bioRxiv - Developmental Biology 2021Quote: ... These lines were routinely passaged every 3-4 days using Versene (ThermoFisher; 15040066). All lines were routinely screened for differentiation and tested for mycoplasma contamination.
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bioRxiv - Neuroscience 2020Quote: ... and 0.175 g/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Invitrogen). Alkaline phosphatase staining reaction was proceeded o/n at RT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Gels were stained for 3 minutes with SYBR Gold (Thermo Fisher Scientific S11494), and the region corresponding to the 18-50 nucleotide sized fragments excised ...
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was done on QuantStudio 3 Real Time PCR Systems (Thermo Fisher Scientific) with the TaqMan gene expression Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... or a positive control probe 5’-5Alexa488N/(ATA)8TUU (ATA)7-3’ (Invitrogen). Reactions were incubated in a water bath at 37°C for 2 hrs ...
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bioRxiv - Microbiology 2022Quote: ... JEG-3 (ATCC) were cultured in EMEM (ATCC) supplemented with 10% FBS (Gibco), penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell counts were performed 3-days after seeding using Trypan Blue (Invitrogen T10282) on Countess slides (Invitrogen C10283 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with siRNA (Table 3) using Lipofectamine RNAiMax (Life Technologies, 13778075) according to the manufacturer’s protocol for transfecting short oligonucleotides ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2021Quote: ... TRPA1−/− male mice were loaded with Fura-2 (3 μg/mL, Life Technologies) and pluronic acid (1:1 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 (high) or 3 (vhigh) ul of RNase I (EN0601, Thermo Fisher Scientific) per 30 ug of RNA were used for digestion ...
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bioRxiv - Molecular Biology 2020Quote: Cells were transfected with plasmid DNA (Supplementary Table 3) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Ca2+ levels were determined by the Ca2+ indicator Fura 3 AM (Thermo Scientific) using confocal microscopy imaging as described previously ...
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bioRxiv - Biophysics 2022Quote: ... DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate salt) was purchased from ThermoFisher scientific (Molecular probes ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Amplification was monitored using the Quantstudio 3 Real time cycler (Thermo Fisher Scientific). ß-globin (primers forward ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 μl of Lipofectamine 2000 in 47 μl of OptiMEM (ThermoFisher Scientific) by pipetting up and down ...
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bioRxiv - Immunology 2020Quote: ... cells were treated with 2 mM caspase-3/7 detection reagent (Fisher Scientific) for 30 min ...