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Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... We used the following Taqman primers from Thermo Fisher (Supplementary Table 3). 18S transcripts were used as the internal control and data were expressed using the 2−ddCt method with Ct limit of 40 ...
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bioRxiv - Molecular Biology 2021Quote: Quantitative RT-PCR was performed on a QuantStudio 3 System (Applied Biosystems) using the PowerUp SYBER Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were coated in 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Percholate (DiI, Invitrogen) for post-procedure visualization of probe placement (Figure 1B) ...
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bioRxiv - Cell Biology 2020Quote: ... and SK-BR-3 cells were maintained in McCoy medium (ThermoFisher Scientific), respectively ...
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bioRxiv - Immunology 2022Quote: ... washed and labeled with Alexa Fluor Dye 488 DBCO (3 μM; ThermoFisher) in 3% BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... and 3 μM CHIR99021 in RPMI supplemented with 1X B27 supplement (Gibco), 1X GlutaMAX (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 volumes of 100% ethanol and 10 μL glycogen (ThermoFisher cat#R0561) were added ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR was performed on a QuantiStudio 3 thermal cycler (Applied Biosystems) from 10 ng of cDNA in a total volume of 20 μL containing Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Capsids were digested with 3 mg/mL proteinase K (Fisher Scientific: BP1700) in 100 mM KCl ...
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bioRxiv - Bioengineering 2022Quote: ... was diluted 1:250 in 3% BSA/0.1% tween-20 (Fisher Scientific) and incubated for 1 h ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs (passages 3-7) were washed with phosphate-buffered saline (PBS, ThermoFisher) and detached by trypsin/EDTA solution (ThermoFisher) ...
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bioRxiv - Biophysics 2022Quote: Samples were run on a 3-8% pre-casted gels (Thermo Scientific), in 1xTA buffer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and 3% horse serum (Gibco by Life Technologies, New York, NY, USA) for 1 h and then incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... in 4:3:1 ratio into 293FT cells (Thermo Fisher Scientific, #R70007) with Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Insulin Receptor β CT-3 mouse monoclonal antibody (1:1000, AHR0271, ThermoFisher), Perilipin-1/PLIN1 D1D8 rabbit monoclonal antibody (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μg/mL anti-CD3 antibody (ThermoFisher Scientific, Cat#16-0038-85), 5 μg/mL anti-CD28 antibody (ThermoFisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... cells were passaged 1:15 every 3 days using trypsin (Gibco, 25300054) and centrifugations at 200 xg ...
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bioRxiv - Immunology 2022Quote: ... 1.5% rat-tail collagen I was prepared from 3% stock (ThermoFisher Scientific), 1N NaOH ...
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The cosmopolitan appendicularian Oikopleura dioica reveals hidden genetic diversity around the globebioRxiv - Evolutionary Biology 2022Quote: ... gDNA was quantified using a Qubit 3 Fluorometer (Thermo Fisher Scientific, Q32850), and its integrity was measured using Agilent 4200 TapeStation (Agilent ...
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bioRxiv - Immunology 2022Quote: ... Approximately 3×106 cells were resuspended in 50 μl sterile HBSS (Gibco) and injected using a 28-gauge insulin needle via the retro-orbital plexus into CD45.1+ C57BL/6 lethally-irradiated recipients ...
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bioRxiv - Microbiology 2022Quote: ... and rescued with GCP medium (15 g/L proteose peptone 3 (ThermoFisher), 1 g/L soluble starch (ThermoFisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... at the 3’-end using terminal deoxynucleotidyl transferase (TdT) (Thermo Fisher, EP0162). Following size-exclusion purification in a Spin-X centrifuge column (Corning ...
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bioRxiv - Pathology 2022Quote: ... 2 and 3 were quantified using CyQUANT Proliferation Assay Kit (Invitrogen, C35011); For myofibroblast differentiation assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Dynabeads with precipitated proteins were washed 3 times with DynaMag-2 (Invitrogen) and eluted with LDS buffer (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... and measured using an ABI QuantStudio 3 sequence detection system (Applied Biosystems). Results were quantified using the 2-ΔΔCt and shown as relative expression from the control groups ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR reaction was performed with a QuantStudio 3 (ThermoFisher) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and labeled using the Pierce RNA 3′ End Desthiobiotinylation Kit (ThermoFisher Scientific). HOXC13-AS pulldown assay was performed using the Pierce Magnetic RNA-Protein Pull-Down Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC-neurons were incubated with 3 µM Fluo-4 (Life Technologies #F14201) at 37°C for 20 min and imaged directly following washout on an LSM Zeiss 880 inverted confocal microscope using a 63×oil 1.4 numerical aperture objective and a 488-nm argon ion laser ...
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bioRxiv - Biochemistry 2022Quote: ... After washing 3 times Alexa Fluor 594 coupled anti-rabbit (Life Technologies) was added at a dilution of 1:500 for 1h at RT ...
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bioRxiv - Cell Biology 2022Quote: ... TOGARAM1 was depleted using siRNA with sequence 5′-CCUCGUAAUUCCUUAGAAA-3′ (Thermo Scientific) Cells were seeded onto coverslips at 70% confluency and transfected with 50 nM of siRNA in two sequential transfections using Lipofectamine RNAiMAX (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear extracts were resolved using 3–8% Tris-Acetate gradient gels (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... and 400 µM 3’-dGTP [>99% purity for UTP and CTP (ThermoFisher); ≥95% purity for 3’-dGTP (Jena Bioscience)] and incubated 2 min at 37°C (to “chase” non-terminated complexes) ...
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bioRxiv - Systems Biology 2022Quote: We prepared fresh 3% low melting agarose (Fisher Scientific, Pittsburgh, PA, USA) in LB in a 70°C water bath ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary cortical neurons were transfected at 3 DIV using Lipofectamine 2000 (Invitrogen) following the manufacturer’s recommendations and the proportion 0.3 μg DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Worm extracts were loaded on NuPAGE 3-8% Tris Acetate gels (Invitrogen). Proteins were then transferred onto nitro-cellulose membranes which were incubated with 1 µg/µL primary antibodies in 1X TBS-Tween (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... fluorescent dextran dyes 3 kDa and 70 kDa (D3329, D1540 respectively; Thermofisher) were added to the top chamber at a concentration of 1 mg/ml ...
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bioRxiv - Pathology 2024Quote: ... PCR cycling was performed on QuantStudio 3 Real-Time PCR system (ThermoFisher) with the following conditions ...
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bioRxiv - Neuroscience 2024Quote: ... or the long TMEM106B 3’ UTR using Lipofectamine 3000 (Thermo Fisher, L3000001). Two days later ...
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bioRxiv - Neuroscience 2024Quote: ... Differentiation was induced sequentially with media A - 3 days (Neurobasal media (Gibco) + N2 supplement (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μm (Dr. Maisch GmbH)) on an EASY-nLC system (Thermo Scientific). The peptides were eluted with an organic solvent gradient from 100% buffer A (0.1% FA ...
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bioRxiv - Pathology 2024Quote: ... PCR cycling was performed on QuantStudio 3 Real-Time PCR system (ThermoFisher) with the following conditions ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed with 3% hydrogen peroxide (Fisher Scientific, Waltham, MA, USA) for 10 minutes to block the activity of endogenous peroxidases ...
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bioRxiv - Molecular Biology 2024Quote: ... the chromatin was incubated for 3 hours with Protein G Dynabeads (ThermoFisher; 10004D ...
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bioRxiv - Immunology 2023Quote: ... Apoptosis was assessed using the CellEvent Caspase-3/7 Detection Reagent (Invitrogen) over TCB-treatment duration or at specific intervals ...
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bioRxiv - Microbiology 2023Quote: ... The Silencer™ siRNA Labeling Kit with Cy™3 dye (Invitrogen) was used to label dsRNA following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Plates were run on QuantStudio 3 Real-Time PCR system (Thermo Fisher). Cycle conditions were as follows ...
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen) and DiD (1,1’- Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; Invitrogen) crystals were inserted under a stereo fluorescence microscope (MZ10 F ...
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bioRxiv - Microbiology 2024Quote: ... for 1h at 37°C and then with 3 µM DAPI (Invitrogen) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 μL of OptiMEM containing 3 μL of PLUS reagent (Thermo Fisher) was mixed with the cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... while EASY-Spray C18 (Thermo Scientific, 15 cm×75 μm, 3 μm) was used as an analytical column ...