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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with TO-PRO-3 Iodide (1:1000, Life Technologies) in 1x PBS for 20 minutes and then mounted with VectaShield mounting media (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... Synthesized cDNA was diluted 1:3 and QS5 or QS6 (Life Technologies) systems were used for performing RT-qPCR analyses ...
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bioRxiv - Microbiology 2024Quote: ... KD cell lines were additionally cultured with 3 µg/ml puromycin (Gibco). ORF11-CRISPR cells have been previously described (Murphy et al. ...
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bioRxiv - Microbiology 2024Quote: ... and the positive reaction was visualized with 3-diaminobenzidine substrate (Thermo Scientific). After counterstaining the specimens with 0.5% methyl green (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was treated with 3 units/mL RNase-free DNase (Ambion) for one hour at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Xanthine and 3-methyl xanthine were purchased from Acros Organics (Antwerpen, Belgium). 1-methyl xanthine and Paraxanthine were purchased from ChemScene (Monmouth Junction ...
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bioRxiv - Immunology 2024Quote: Data was analyzed with Compound Discoverer 3 (Thermo Fisher Scientific, Dreieich, Germany). Features were grouped and identified with a mass tolerance of 5 ppm and a retention time tolerance of 0.2 min ...
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bioRxiv - Immunology 2024Quote: ... and blocked using PBST supplemented with 3% non-fat milk (Life Technologies). Monoclonal antibodies were diluted to 30 μg/mL and then serially diluted 1:3 to a final concentration of 1.52x10-3 μg/mL in PBST supplemented with 1% non-fat milk (Life Technologies) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were run on NuPAGE 3-8% Tris Acetate gradient gels (Invitrogen) at a constant voltage ...
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bioRxiv - Immunology 2024Quote: ... and added 3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] (Life Technologies, Carlsbad, CA, USA) diluted to 5 μg/mL in RPMI 1640 ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR (qPCR) assays were performed using Quant Studio 3 (Applied Biosystems). A total volume of 25 μL PCR mixture included 12.5 μL of SYBR Green PCR master mix (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... Cells were then mixed in with 3% methylcellulose (Gibco, cat # 25030-081) and loaded into a 250μL Gastight syringe (Hamilton ...
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bioRxiv - Molecular Biology 2024Quote: ... and regularly checked for IL-3 addiction by performing 3x PBS (Gibco) washes and outgrowth in the absence of IL-3 to confirm cell death in the parental ...
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bioRxiv - Neuroscience 2024Quote: ... This medium was exchanged 2-3 hours after plating with Neurobasal (Gibco) supplemented with L-glutamine (2 mM) ...
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bioRxiv - Cancer Biology 2024Quote: ... Then sections were incubated in 3% H2O2 (30% H2O2 (Thermo Scientific #10687022) diluted in TBS ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA was isolated from 3 dpf TL embryos (RNeasy, Qiagen; RNAlater, Invitrogen), and AffinityScript QPCR cDNA Synthesis Kit (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... MMP-3 and TNF-α (Procarta Multiplex Assay, Thermo Fisher Scientific, USA) levels by Luminex multiplex according to manufactures’ instructions.
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed using the Quantstudio 3 apparatus (Thermo Fisher Scientific) with GoTaq qPCR master mix 2X with SYBR Green (Progema ...
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bioRxiv - Molecular Biology 2024Quote: Cells were transfected with plasmid DNA (Table 3) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in the second cross-linking solution - 3 mM DSG (ThermoFisher 20593) in DPBS – and incubated for 45 min at 25°C with agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... and cel-miR-54-3p (C. elegans sequence: 3’-UACCCGUAAUCUUCAUAAUCCGAG-5’, Invitrogen) were added to each sample and used as external controls.
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bioRxiv - Molecular Biology 2024Quote: ... in a QuantStudio 3 Real-Time PCR detection system (Thermo Fisher Scientific). For each sample tested ...
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Tumor Spheroid Uptake of Fluorescent Nanodiamonds is limited by Mass Density: a 4D Light-Sheet AssaybioRxiv - Biophysics 2024Quote: Fully formed spheroids were rinsed 3 times with phosphate buffer saline (Gibco) and were supplemented with a cell impermeable dye (Alexa Fluor 488 Hydrazide ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were counted using a Countess 3 Automated Cell Counter (Thermo Fisher) by performing four counts on the same chip and using the average cell number to increase seeding precision ...
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bioRxiv - Cell Biology 2024Quote: ... on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). EBOV or MARV standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g.
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bioRxiv - Immunology 2024Quote: ... at a DNA mass ratio of 3:1 in Opti-MEM (ThermoFisher). After a 10 minute incubation ...
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bioRxiv - Immunology 2024Quote: ... 3×105 cells/ml were seeded and cultured in RPMI 1640 (Invitrogen) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... NRDE-3-bound RNAs were isolated using TRIzol reagent (Thermo Fisher, 15596018), followed by chloroform extraction and isopropanol precipitation ...
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bioRxiv - Bioengineering 2024Quote: ... mice were injected intraperitoneally with 3 mg D-Luciferin (Thermo Fisher, 88291) in 200 μl 1X PBS ...
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bioRxiv - Genetics 2024Quote: ... and separated on 3%–8% Tris-acetate NuPAGE precast gels (Life Technologies) at 150 V for 70 min ...
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bioRxiv - Neuroscience 2024Quote: ... TO-PRO-3 (1:3000; Thermo Fisher Scientific, cat. #T3605, Waltham, USA) or DAPI (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... Samples were then run in triplicate on the QuantStudio 3 (Applied Biosystems) using the default Fast program ...
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bioRxiv - Biophysics 2024Quote: ... and then transferred to the membrane using iBlot 3 Transfer Stacks (Invitrogen). Blocking was performed for 1 hour with 5% nonfat dry milk in TBST and blotting was performed with primary antibodies overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... were cleaned and silanized with 1% 3-aminopropyl-trimethoxysilane solution (Acros Organics) for 10 min and then treated with 0.5% glutaraldehyde (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and regularly checked for IL-3 dependence by performing 3x PBS (Gibco) washes and outgrowth in the absence of IL-3.
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bioRxiv - Cell Biology 2024Quote: ... 3-4 or 5-6 and Phusion DNA polymerase (Thermo Fisher Scientific). The resulting PCR mix was DpnI digested and 4 μl of the mix was used for transformation of E ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were run in triplicate on a QuantStudio 3 (ThermoFisher Scientific). No template controls were included in each PCR run.
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bioRxiv - Cell Biology 2024Quote: ... and TaqmanTM Gene Expression assays (Thermo Fisher Scientific, listed in Table 3). Three technical replicates were performed for all qPCR experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... Calu-3 cells were maintained in DMEM low glucose medium (Gibco, 31885023) supplemented with 10% FBS and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then analysed using Countess™ 3 Automated Cell Counter (Invitrogen) to determine cell survival.
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bioRxiv - Evolutionary Biology 2024Quote: ... The mounting slide (3’’ x 1’’ x 1mm, Fisher Scientific, Pittsburgh, PA) was placed on top of the cover slide to finish preparation ...
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bioRxiv - Cell Biology 2024Quote: ... 3 uL GlycoBlue Coprecipitant (final conc. 75 ng/uL) (Thermo Fisher Scientific), 50 mM NaCl ...
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bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic stem cell (hESC; H9)-derived neural stem cells (NSC) (Thermo Fisher Scientific) were cultured ...
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bioRxiv - Microbiology 2020Quote: A549 human epithelial cells (ATCC ref# CCL-185) were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Cell Biology 2020Quote: ... Taqman probes targeting human GAPDH (Hs02758991_g1) and IFIT1 (Hs03027069_s1) were purchased from Life Technologies. qRT-PCR data were collected on a StepOnePlus™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: Membranes were coated with an anti-human IgG capture antibody (Thermo Fisher Scientific, A18813). Cells were then added to the plate in duplicate in 2 fold serial dilutions and incubated for 24hrs prior to detection using an alkaline phosphatase-linked anti-human IgG detection antibody (Jackson ...