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Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1 (Invitrogen). All experimental conditions were carried out in quadruplicates ...
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bioRxiv - Molecular Biology 2021Quote: ... Between 25-35ug nuclear extract was separated on home-made SDS-PAGE gels or NuPAGE™ 3-8% Tris-Acetate gels (Life Technologies, for large Mediator subunits). Gels were blotted onto nitrocellulose membranes using the Trans-Blot Turbo transfer system (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100, Biolegend; CD140a-APC 1:100, Thermo Fisher; CD29-APCe780 ...
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bioRxiv - Developmental Biology 2020Quote: ... HNFα4 (Proteintech, #26245-1-AP, 1:500, Albumin (Bethyl, #A80-129A, 1:500, AFP (Thermo Scientific, #RB-365-A1, 1:500), ki-67 (8D5 ...
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bioRxiv - Microbiology 2019Quote: ... the suspension was further diluted 1:50 in growth medium and mixed 1:1 with 1% agarose (TopVision Low Melting Point Agarose, Fisher Scientific). Each chamber in the fluidic chip was loaded with 5 μL bacteria-agarose and incubated at 4 °C for 5 min to solidify the agarose gel ...
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bioRxiv - Plant Biology 2022Quote: In vitro transcription was initiated with the addition of 9.6 µL of MegaScript T7 IVT mix (1:1:1:1:1:1 of CTP solution, GTP solution, UTP solution, ATP solution, 10X Reaction Buffer, T7 Enzyme Mix; ThermoFisher Scientific) to the sample then incubated at 37□ overnight ...
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bioRxiv - Neuroscience 2023Quote: ... consisting of N2B27 medium (DMEM/F-12 – Neurobasal in 1:1 ratio, 1% B27 w/o Vitamin A, 0.5% N2, 1% Penicillin-Streptomycin, all from Thermo Fisher Scientific) supplemented with 3 μM CHIR ...
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bioRxiv - Neuroscience 2023Quote: ... N2B27 medium (DMEM/F-12 – Neurobasal in 1:1 ratio, 1% B27 w/o Vitamin A, 0.5% N2, 1% Penicillin-Streptomycin, all from Thermo Fisher Scientific) supplemented with 3 μM CHIR ...
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bioRxiv - Cancer Biology 2023Quote: ... or 647 were stored at -20 °C diluted 1:1 in glycerol and used at 1:500 dilution in Abdil for 1 hour at room temperature (Invitrogen Molecular Probes A11029 ...
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bioRxiv - Immunology 2024Quote: ... have been incubated with 10 μg of either anti-BCL-3 (SantaCruz, sc-32741) or it’s corresponding isotype control antibody (Armenian Hamster IgG, Thermo Fisher Scientific, 16-4888-85) overnight at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... a 6 well plate containing 6 mL FACS Clean (Thermo Fisher, BD 340345), 6 mL FACS Rinse (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... and labelled with 0.7 μM 6-carboxyfluorescin dictate (6-CFDA; Thermo Fisher Scientific) for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... high dose IL-6 stimulation received 100 ng/ml IL-6 (Gibco; PHC0066) or 100 pM acetic acid vehicle stimulation for either 3-or 24-hours and immediately collected for analysis ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Microbiology 2021Quote: ... was added to the Lysing Matrix E tube and supplemented with 6 µL of lysozyme (50 mg mL-1, Thermo Fisher Scientific, Cat. no. 90082), followed by a 60 min incubation at 37°C with mixing at 300 rpm ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM) and underwent blasticidin (ThermoFisher Scientific; 10 μg/ml) selection for 6 days ...
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bioRxiv - Genetics 2019Quote: ... One μL of diluted cDNA (1:25) was used for real-time PCR in a QuantStuioTM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, California, USA). Peptidylprolyl isomerase A (PPIA ...
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bioRxiv - Cancer Biology 2023Quote: ... in PBST and stained for 1 hour with primary antibodies (ZEB1: Bethyl, IHC-00419; E-Cadherin: BD Biosciences, 610181; Collagen 6: Abcam, ab182744; αSMA: Thermo Fisher Scientific, 14-9760-82) and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs ...
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bioRxiv - Neuroscience 2023Quote: ... Fetal brains were evaluated by Western blot (corticotropin releasing factor receptor 1 [CRFR1]; glucocorticoid receptor [GR], interleukin [IL]-6 receptor [IL-6R]; IL-17A and β-actin, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: ... and TagRFP-T-VAPB(1-218)-eMagB or TagRFP-T-eMagB-PHOSBP (prey) and iRFP-P4C at a 3:2:1 ratio in OptiMEM-I (Thermo Fisher Scientific) (1:4 DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Fresh human NK cells were cultured for 3 days at a density of 1×106 cells/ml in RPMI 1640 medium (Gibco, Thermo Fisher) containing 10 ng/ml recombinant human IL-2 (Gibco ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We generated expression constructs using a 2876 bp promoter for the C isoform of kcnl-1 and a 3’ UTR from unc-54 by three-fragment Gateway cloning (Thermo Fisher, USA). See Table S2 for details ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were washed (PBS + 0.1% Triton X-100 for 3×15 minutes at RT) before applying the corresponding secondary fluorescent antibodies (Invitrogen, USA; 1:1000) (in PBS + 0.1% Triton X-100 + 10% FBS for 1 h at RT in darkness).
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in a ratio of 3:1 and total RNA was extracted with Ribopure Yeast Kit (Ambion, Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3×5mins in PBS and incubated for two hours in appropriate secondary antibodies (Invitrogen; 1:250 in blocking solution). For double labelling of Iba1 and GAD67 ...
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bioRxiv - Cell Biology 2019Quote: ... HACAT and KB 3-1 cells were seeded into white 1536-well plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 250 ...
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bioRxiv - Developmental Biology 2019Quote: ... and biotinylated at a molar ratio biotin/protein (3:1) for 30 min at room temperature (EZ-Link NHS-PEG4-Biotin (1189-1195, Fisher Scientific, Germany)) ...
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bioRxiv - Cell Biology 2019Quote: ... RNA interference was done by transfecting 100 nM siRNA (TPD54 1: GUCCUACCUGUUACGCAAU, TPD54 2:CUCACGUUUGUAGAUGAAA, TPD54 3:CAUGUUAGCCCAUCAGAAU) with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then washed 3 times with PBS + TritonX and a secondary anti-mouse antibody Alexa Flour 488 (Life Technologies: 1:1000) was added for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue sections were then washed 3× 10 min with PBS and incubated with the appropriate AlexaFluor-conjugated secondary antibody (1:200; Invitrogen/ThermoFisher Scientific) for 2h ...
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bioRxiv - Biochemistry 2020Quote: ... N-((6-(2,4-DNP)Amino)Hexanoyl)-1-(BODIPY™ FL C5)-2-Hexyl-Sn-Glycero-3-Phosphoethanolamine (PED-A1) and BODIPY™ FL C5 were purchased from Thermofisher scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Dishes were washed 3 times with PBS-Triton X and incubated with Alexa-Fluor 488 conjugated secondary antibody 1:1000 (Molecular Probes-Invitrogen) in dilution buffer for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The brain slices were then transferred into a 3% Triton-X PB solution supplemented with Alexa Fluor 555 streptavidin (1:1000, Thermo Fisher Scientific) and DAPI (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were adjusted to 1-3 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007) (Thermo Fisher Scientific) was added to lysates ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μl of a solution at 3 µg/mL of mouse anti-AP (IgGAb-1 clone 8B6.18 Thermo Fisher Scientific; Waltham, MA) in 1X PBS was added to each well of 384-well plates using an automated multichannel pipette (Viaflo Assist ...
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bioRxiv - Neuroscience 2019Quote: ... DRAQ7 was added to each well at a concentration of 3 µM together with Hoechst 33342 (1:1000 Thermo Fisher Scientific, H1399) and incubated for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: Un-stimulated and LPS-stimulated cells were washed 3 times with 1x external solution and then incubated with CTxB conjugated to AlexaFluor 555 (1:500, Invitrogen, C-34776) +/− LPS in 1x external solution with rotation for 30 minutes at 4°C to limit internalization ...
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bioRxiv - Biochemistry 2021Quote: ... washed 3 times with blocking buffer and incubated for 2 h with Alexa 647 anti-rabbit (1/250, A21247, Thermo Fisher Scientific). After washing the cells 3 times with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... approximately 1 pmol injected onto a Acclaim PepMap 100 guard column (75 μm×2 cm C18, 3 μm particles, 100 Å; Thermo Scientific) and separated on a 50 cm PepMap RSLC C18 (75 μm×50 cm ...
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bioRxiv - Neuroscience 2020Quote: ... Then the sections were washed 3x 10 min at RT in PBS and incubated on a shaker for 3 hours at RT with secondary reagents: streptavidin Alexa Fluor® 405 (1:200, Life Technologies (Invitrogen), S32351 ...
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bioRxiv - Microbiology 2020Quote: Cell viability was assessed using the 3-(4,5-dimethylthiazo-1-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Thermo Fisher Scientific, Waltham, MA, USA). A549 cells were seeded independently in 96-well tissue culture plates (10,000 cells/well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested using 0.25% Trypsin-EDTA and washed with 3 mL of 1% BSA (Capricorn, #Cat no: BSA-1s) in PBS (Gibco, #Cat no:003002) and centrifuged at 1500 rpm for 5 mins ...
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bioRxiv - Cell Biology 2021Quote: ... polyadenylated mRNA was isolated from 1-3 µg of high-quality total RNA using the Dynabeads mRNA DIRECT Micro kit (Thermo Fisher Scientific). The mRNA was subsequently fragmented and used for cDNA synthesis and library preparation using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed with TBS-BSA + 0.1% Triton X-100 for 3 × 10 minutes with vigorous shaking and then mounted using ProLong® Gold antifade reagent (Molecular Probes). For visualization of Mad2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Light trypsinization consisted of a 3 min incubation with 1 mL of trypsin (Trypsin-EDTA 0.25%, Gibco™/ ThermoFisher, cat. no. 25200056), followed by washing with complete medium ...
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bioRxiv - Cancer Biology 2021Quote: ... Light trypsinization consisted of a 3 min incubation with 1 mL of trypsin (Trypsin-EDTA 0.25%, Gibco™/ ThermoFisher, cat. no. 25200056), followed by washing with complete medium ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were further cultured for an additonal 3 days to induce hepatic endodermal cells in RPMI 1640 medium supplemented with 1% B27 (Thermo Fisher Scientific), 20 ng/mL bone morphogenetic protein 4 (BMP4 ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Cloned mAb vectors for IgG1 heavy chain and Kappa or Lambda light chains were co-transfected at a ratio of 1:3 (H:K/L) into Expi293F cells (Thermo Fisher Scientific Inc.) using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Microbiology 2021Quote: ... total nucleic acids were extracted followed by reverse transcription with random hexamers and oligo-dT priming (ratio 3:1) using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific). The generated cDNA was used as input for 14 overlapping PCR reactions (ca ...