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Citations for Thermo Fisher :
1001 - 1050 of 1284 citations for Fmoc Lys Boc Rink Amide Resin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... charged by Ni2+ ions or 1 ml Tricorn 5/10 column (Cytiva) packed by Ni-NTA resin (Thermo Fisher Scientific). Columns were equilibrated by the 50 mM Na-PO4 ...
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bioRxiv - Immunology 2020Quote: Serum antibodies were purified from 25mL of remaining serum of final bleeds using antigen conjugated to NHS-activated agarose resin (ThermoFisher). Columns were washed three times with 1X PBS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... the anti-camel antibody-resin was vortexed vigorously and 300 ul was aliquoted into the wells of a 96 well plate (Nunc™ 96-Well Polypropylene DeepWell™ Storage Plates) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Cell debris were removed by centrifugation and supernatants were incubated with 1 ml of HisPur Ni-NTA resin (Life Technologies), previously washed 3 times with Buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... Synthetic peptides (60 nmol each) were incubated with 18 μL streptavidin agarose resins (high capacity streptavidin agarose, Thermo Fisher Scientific) per experiment for 2 hr at room temperature in 80 μL of lysis buffer (1% NP-40 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then incubated overnight at 4°C and under agitation with 2 mL Ni-NTA resin (50% v/v, Thermofisher) pre-equilibrated with lysis buffer.
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bioRxiv - Microbiology 2021Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin for 2 h, then filter sterilized and supplemented with EDTA (Invitrogen) to 1 mM) ...
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bioRxiv - Microbiology 2020Quote: ... The supernatants were filtered through 0.4 μm syringe filters and loaded onto 10 mL spin columns with 2 mL HisPur cobalt resins (89964, Thermo Scientific). For purification ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were then incubated with streptavidin agarose resin (30 μL of 1:1 beads:lysis buffer slurry) (Thermo scientific, cat#20349) for 1.5 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: 100 μg of protein extracts were incubated with freshly washed Pierce Streptavidin Plus Ultra-Link Resin (Thermo Scientific, Cat. #53117) in protein binding buffer [150 mM NaCl ...
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bioRxiv - Systems Biology 2022Quote: ... 130 μl was incubated with equal volume (130 μl) of High Select Top14 Abundant Protein Depletion Resin (ThermoFisher Scientific, A36372) at room temperature in centrifuge columns (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were first purified by affinity chromatography on a Ni-nitrilotriacetic acid (Ni-NTA) agarose resin (Thermo fisher scientific). The column was washed with buffer A supplemented with 20 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by spinning for 15 minutes at 25,000 rcf and the retained supernatant was flowed twice over HisPur Cobalt Resin (Thermo Scientific) pre-equilibrated with RecA Lysis Buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was filtered through 5 µM and 1.2 µM filters and applied to a column containing SulfoLink resin (ThermoFisher, 20402) coupled to PDZ ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated GPC-I53-50As and Fabs of 12.1F and S370.7 were purified by rolling the culture supernatant overnight at 4°C with Ni-NTA Agarose resin (Thermo Scientific). The next day ...
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bioRxiv - Biochemistry 2022Quote: ... Transfected supernatants were harvested and filtered five days post-transfection and antibodies were purified using Protein A resin (Thermo Fisher). Eluted antibodies were then buffer exchanged into PBS.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Peptides were separated on an in-house packed 100 μm inner diameter column packed with 35 cm of Accucore C18 resin (2.6 um, 150 Å, ThermoFisher), using a gradient consisting of 5–35% (ACN ...
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Immunology 2022Quote: ... Undigested IgG and Fc fragments were removed by a 2 h incubation with CaptureSelect IgG-Fc resin (Thermo Fisher Scientific). Resin was spun down and the supernatant run on a Superdex 200 increase 10/300 GL column (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Control pull-down contained lysate and 40ul of high-capacity streptavidin agarose resin (Thermo Fisher Scientific, 20357, 50% w/v), whereas the nanobody condition contained the same beads plus 2μg of biotinylated Nanoglue ...
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bioRxiv - Systems Biology 2024Quote: The plasma-resin mixture is then transferred to a Pierce™ Micro-Spin Column (Thermo Fisher Scientific, Waltham, United States) placed in a 2mL Protein LoBind® Eppendorf tube and centrifuged at 1000g for 2 minutes at RT (Microcentrifuge 5415 ...
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bioRxiv - Immunology 2024Quote: ... was dissolved into the reaction before loading the digested IgG mixture over a CaptureSelectTM IgG-CH1 affinity resin (ThermoFisher Scientific) to bind the Fabs ...
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bioRxiv - Immunology 2024Quote: Total IgG from rat serum was purified on drip columns packed with Pierce Protein G agarose resin (Thermo Fisher Scientific). Pierce protein G IgG binding buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). The peptides were separated using a 180 min linear gradient from 5% to 32% buffer B (90% ACN + 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified at 4°C by the batch method over 1 mL Ni-NTA resin according to the manufacturer’s instructions (HisPur, Thermo Scientific). All buffers were 50 mM NaPO4 ...
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bioRxiv - Cancer Biology 2024Quote: ... the resin underwent trypsin digestion and peptides were run on an Orbitrap Fusion™ Lumos™ mass spectrometer (ThermoFisher Scientific) at the UNMC Proteomics Core and analyzed using Proteome Discoverer (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international). TALON® Metal Affinity Resin was purchased from Takara Bio (Mountain View ...
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bioRxiv - Cell Biology 2024Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 180 min per fraction ...
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bioRxiv - Cell Biology 2024Quote: ... A 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 180 min per fraction ...
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bioRxiv - Genetics 2024Quote: ... tryptic peptides were then separated by reversed-phase XSelect CSH C18 2.5-μm resin (Waters) on an in-line 150 x 0.075–mm column using an UltiMate 3000 RSLCnano system (Thermo Fisher). Peptides were eluted using a 60-minute gradient of buffer A/buffer B from 98:2 to 65:35 ...
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Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... The 100 μm capillary column was packed with 35 cm of Accucore 150 resin (2.6 μm, 150 Å; ThermoFisher Scientific).
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bioRxiv - Biophysics 2024Quote: Thermo Scientific™ HisPur™ Ni-NTA Resin used in the IMAC purification step was purchased from Fisher Scientific (#88221). Prepacked SP Ion Exchange column used in the pNTS1(H8-Ctail ...
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bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant after centrifugation was applied to a gravity-flow immobilized metal ion affinity chromatography (IMAC) column (HisPur cobalt resin, ThermoFisher). The column was washed with buffer B (50 mM Tris/HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: Endotoxin levels in OVA-ZE and pZR-ELP were reduced by Pierce™ High Capacity Endotoxin Removal Resin (Thermo Fisher). To confirm low endotoxin content within proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto a 100 µm (inner diameter) capillary column (∼30 cm) packed in-house with C18 resin (2.6 µm, 150Å, ThermoFisher Scientific). Peptide fractions were separated with a 4 h acidic acetonitrile gradient from 5-35% Buffer B (Buffer A = 0.125% formic acid ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 150 Å, ThermoFisher). For each analysis ...
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bioRxiv - Immunology 2023Quote: ... or immunoaffinity chromatography (D1.3 for HEL and 2G12 for gp120) where columns were prepared with AminoLink Plus resin (Life Technologies), both used according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... packed with Halo C18 reversed-phase resin (2.7 µm particle size, 90 nm pore size, MichromBioresources.) was used with EASY-nLC 1200 system (Thermo Fisher). The nanospray ESI was fitted onto the Thermo Q-Exactive plus mass spectrometer (Thermo Electron ...
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bioRxiv - Biochemistry 2022Quote: ... The filtrate was then incubated for 1 h rotating at 4 °C with 2 mL Ni-NTA resin (Thermo Fisher) per 1.5 L of expression culture ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting lysate was centrifuged at 40,000 g and supernatant was loaded onto 6 ml of HisPure Ni-NTA resin (Thermo Scientific). The column was then washed with 7-column bed volumes (CV ...
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bioRxiv - Plant Biology 2023Quote: ... 75 μm inner diameter C18-reversed phase column packed with 3 μm resin (EASY-Column Capillary Columns, Thermo Fisher Scientific) in buffer A (2% [v/v] acetonitrile and 0.1% [v/v] formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... A 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 90 min per fraction ...
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bioRxiv - Molecular Biology 2023Quote: ... The 100 µm capillary column was packed in-house with 35 cm of Accucore 150 resin (2.6 μm, 150Å; ThermoFisher Scientific). Data were acquired for 120 min per fraction ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was spun at 20,000 rpm for 45 min to remove cell debris and the supernatant was applied to a column packed with HisPur Ni-NTA Agarose Resin (5 mL, ThermoFisher) and equilibrated with binding buffer (50 mM Tris HCl ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysates were passed through a 0.45-μm filter and incubated with 1 ml of Ni-NTA (nitrilotriacetic acid) resin (Thermo Scientific) at 4°C over-night (O/N ...
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bioRxiv - Neuroscience 2023Quote: ... gradient on a 35-40 cm long 100 µm inner diameter column packed with Accucore (2.6 μm, 150Å) resin (ThermoFisher Scientific). For cortex slices the FAIMS voltages were set at −40 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV particles were captured by affinity chromatography using POROS™ CaptureSelect™ resins (AAV9 and AAVX, Thermo Fisher Scientific, MA). AAV suspension was concentrated using centrifugal devices (Amicon® Ultra-15 100 kDa centrifugal filter devices ...