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Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations for TRAP 5 amide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... A maximum of 5×103 live CD3+CD4+ T cells was sorted into 100 μL of RNAlater (Ambion) using a FACSAria III (BD Biosciences) ...
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bioRxiv - Biochemistry 2019Quote: ... Reduction of disulfide bonds was performed with 5 mM Tris(2-carboxyethyl)phosphine (TCEP) Bond-breaker (Thermo Scientific) at RT for 1 h ...
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bioRxiv - Immunology 2019Quote: ... counted and plated at a density of 5×105 cells/well (24 well-plates) in RPMI 1640 (Gibco) 10% FBS (Dutcher) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was synthesized by reverse transcription from 5 μg RNA using First Strand cDNA Syntesis Kit (Thermo Scientific). RTqPCR was performed on a 7500 Fast System (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: Weaned mice were ear-notched and samples were incubated in lysis buffer (100 mM Tris HCl pH 8.5, 5 mM EDTA, 200 mM NaCl, 0.2% SDS, 0.14 mg/mL Proteinase K, Ambion™) for 2 hours at 55°C under agitation (700 rpm) ...
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bioRxiv - Microbiology 2019Quote: ... RNA (5 ng µl−1) was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). 1 µl of cDNA was used as a template in a 10 µl qRT-PCR reaction performed with Power SYBR reagent (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... One million cells were suspended in 5 milliliters of tenogenic growth media comprising high glucose DMEM (Thermo Scientific), 10% Cellect™ Silver fetal bovine serum (MP Biomedicals) ...
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bioRxiv - Physiology 2020Quote: ... Larvae were sampled and placed in a liquid scintillation vial with 5 ml of EcoScint cocktail (Fisher Scientific). Samples were counted in a Beckman (Brea ...
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bioRxiv - Molecular Biology 2020Quote: Modified 5’ RLM RACE was performed according to the manufacturer’s instructions (FirstChoice™ RLM-RACE kit, ThermoFisher Scientific). In order to detect premature miRNAs ...
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bioRxiv - Immunology 2019Quote: ... and infected intravaginally with 5×105 FFU of PRVABC59 ZIKV in 15 µL of HBSS (Gibco, Thermo Fisher). Mock-infected mice received 15 µL of HBSS intravaginally ...
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bioRxiv - Immunology 2019Quote: ... and infected intravaginally with 5×105 FFU of PRVABC59 ZIKV in 15 µL of HBSS (Gibco, Thermo Fisher). Mock-infected mice received 15 µL of HBSS intravaginally ...
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bioRxiv - Biophysics 2019Quote: ... Nick translation was initiated by the addition of 5 µl of 250 µM biotin-11-dUTP (ThermoFisher Scientific) and 1.5 U of E.coli DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... YSM tissues from day 5 embryos were treated with collagenase (collagenase type 4, 17104019, ThermoFisher, Waltham, MA, USA) to partially digest the extracellular matrix and facilitate cell isolation [17] ...
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bioRxiv - Cell Biology 2019Quote: ... The membrane was blocked with 5% BSA (GenDEPOT, A0100) in 1% TBST (Fisher Scientific, BP2471-1, BP337-100) overnight ...
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bioRxiv - Microbiology 2019Quote: ... DNA was removed from 5 µg of total RNA using the TURBO DNase kit (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2019Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing 10K MWCO (Thermo Scientific) using a buffer that is high in salt and contains a reducing agent (buffer 1 ...
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bioRxiv - Immunology 2019Quote: ... were then infected with EV71 viral isolates at MOI 5 in serum-free Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco) (50) ...
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bioRxiv - Microbiology 2019Quote: Ligation reactions were performed with a 1:5 ratio of vector:insert using T4 DNA Ligase (15224017, ThermoFisher Scientific) in a 20ul reaction as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were allowed to attach overnight prior to transfection of plasmid DNAs (0.1-0.2 μg/well) using Lipofectamine 2000 (2-5 μL/well; Invitrogen) within a small volume of Opti-MEM (200 μL ...
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bioRxiv - Immunology 2019Quote: Bacterial cultures were incubated O/N in a 5 μM solution of Draq5 Fluorescent Probe Solution (ThermoFisher Scientific). Bacterial suspensions were washed 3 times with PBS before RAW264.7 macrophages were infected.
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bioRxiv - Cancer Biology 2019Quote: ... The cell suspension was filtered through a 70 μm cell strainer and centrifuged at 1200 rpm for 5 min and the pellet was re-suspended in 0.9 M sucrose in 0.5 x HBSS (Gibco). After further centrifugation for 20 min at 2000 rpm ...
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bioRxiv - Plant Biology 2019Quote: ... Total RNA samples from approximately 5 x 108 cells were extracted using mirVana™ miRNA Isolation Kit (Ambion). RNA concentration was measured by measuring absorbance at 260nm using the Eppendorf Biospectrometer ...
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bioRxiv - Bioengineering 2019Quote: ... HS-27a and HS-5 cell lines were cultured in RPMI 1640 (Life Technologies, Villebon-sur-Yvette, France) and hMSCs and MS-5 in MEM Alpha (Life Technologies) ...
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bioRxiv - Genomics 2019Quote: ... and washed in Buffer F (50 mM Tris-HCl pH 8.0, 40% glycerol, 5 mM MgCl2, 0.5 mM DTT, 4 u/mL RNase inhibitor [SUPERaseIN, Ambion]). Washed permeabilized cells were finally resuspended in Buffer F at a density of 1×106 cells/30 μL and immediately frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... 293-T-REx™ cells were cultured in the presence of 5 µg/mL blasticidin (Gibco; #A11139-03) to maintain the pcDNA™6/TR plasmid carrying the ORF for tetracycline (Tet ...
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bioRxiv - Developmental Biology 2020Quote: ... blastocysts were incubated in PBS containing 1 mM EDTA for 5 min followed by incubation in 0.05% (w/v) trypsin-0.53 mM EDTA solution (Invitrogen) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... 18 and 20 hpf in 5 ml borosilicate glass tubes (Fisher Scientific, Waltham, MA. Cat.No. 14-961-26) and washed with 2 ml calcium- and magnesium-free artificial seawater (CMF-ASW ...
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bioRxiv - Cell Biology 2019Quote: ... SUM159 cells were grown at 37°C and 5% CO2 in DMEM/F-12/GlutaMAX (GIBCO, Langley, OK), supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2021Quote: ... HCT116 cells were incubated at 37°C with 5% CO2 and cultured in Dulbecco’s Modified Eagle Medium (GIBCO) medium ...
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bioRxiv - Genetics 2021Quote: Embryo pellets were resuspended 100uL minimal salts solution (M9)5 and 1ml of TRIzol® Reagent (ThermoFisher 15596026) then snap-frozen in liquid N2 and stored at −80°C until all samples were collected ...
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bioRxiv - Genetics 2020Quote: ... Then 5 × 106 cells were used to perform β galactosidase activity using Tropix Gal-Screen System (Applied Biosystems). The readings were normalized to MTG16 wild type activity ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with PBS four times and blocked using 1% BSA and 5% normal goat serum (Invitrogen) in PBS for 1 hour ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the putative Y-specific BAC labeled with ChromaTide Alexa Fluor 568-5-dUTP (Invitrogen, Carlsbad, CA, USA). Starting with these verified Y-specific BACs ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 37°C in a 5 % CO2 humidified atmosphere using MCDB-131 medium (Gibco, Ireland) with phenol red and supplemented with 15 % fetal bovine serum (Natocor ...
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bioRxiv - Immunology 2021Quote: ... the cell pellets were resuspended in 5 mL of commercially available 1x Red Blood Cell Lysis buffer (ThermoFisher) for 3 minutes ...
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bioRxiv - Microbiology 2021Quote: ... A2’p5’A standard was prepared by incubating 5’pA2’p5’A with shrimp alkaline phosphatase (ThermoFisher, USA) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Both human and simian hepatocytes were maintained at 37 °C in 5% CO2 in William’s E medium (Gibco) supplemented with 10% fetal clone III serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: ... After 30 min of incubation at 37 °C and 5 % CO2 in a humid atmosphere in DMEM (Invitrogen), hemispheres were fixed in 4% PFA (in 1X PBS ...
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bioRxiv - Biochemistry 2020Quote: ... were cultivated at 37 °C and an atmosphere containing 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) containing 10% fetal bovine serum (Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were maintained at 37°C with 5% CO2 in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... were coated with 5 μg/mL anti-DPEEAE neoepitope antibody (Cat n. PA1-1748A, Life Technologies, Paisley, UK) in carbonate buffer pH 9.6 (16 h ...
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bioRxiv - Biochemistry 2020Quote: ... Reverse transcription was initiated by incubating with 5 µL Superscript™ reverse transcriptase (200 U/µL) (Fisher Scientific) for 50 min at 42°C ...
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bioRxiv - Bioengineering 2020Quote: ... hBMSCs (passage 3) were expanded at 37°C and 5% CO2 in expansion medium (Dulbecco’s modified Eagle’s medium (DMEM; GIBCO) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: All cells were maintained at 37 ± 0.5 °C in a humidified 5% CO2 incubator (Isotemp, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2021Quote: ... All samples were then incubated with nucgreen™ Dead 488 (Invitrogen™, 1 drop/5 ml in PBS) overnight for spheroids and 4 h for cell monolayers ...
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bioRxiv - Bioengineering 2020Quote: ... and fusion medium (low-serum medium - 94 % Dulbecco’s Modified Eagle’s Medium, Gibco, 5 % horse serum, 1 % antibiotic/ antimycotic Gibco) were changed every other day ...
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bioRxiv - Bioengineering 2019Quote: Cells were cultured in a humidified atmosphere with 5% CO2 at 37°C in Dulbecco’s modified Eagle’s medium (DMEM GlutaMAX, Gibco) with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were cultured in a humidified atmosphere with 5% CO2 at 37°C and high glucose Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... by incubating muscles twice for 45 min in preoxygenated Ringer containing 5 μM Rhod-3 AM (Life Technologies), 0.02 % Pluronic acid (Life Technologies ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...