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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... peptides were loaded on a C18 PepMap100 pre-column (300 μm i.d. x 5 mm, 100Å, Thermo Fisher Scientific) at a flow rate of 12 μL/min in 100% buffer A (0.1% FA in water) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were washed 3 times with WB and nuclei were stained for 5 min with Hoechst 33342 (Life Technologies) in WB ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% w/v sodium dodecyl sulfate) to which 400 μl of warm 5:1 Acid Phenol:Chloroform (Ambion, cat. # AM9722) were added and the resulting phase-separated mixture was agitated by vortexing at maximum speed for 15 sec ...
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bioRxiv - Biochemistry 2020Quote: ... 5 x 0.3mm) at a flow rate of 1.5 μl/min using an UltiMate 3000 LC (Thermo Scientific, Massachusetts). The trap was washed for 4 min with 0.1% formic acid in 90% ACN (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... CA) and maintained at 37 °C and 5% CO2 in complete medium: Dulbecco’s modified Eagle’s medium (DMEM, Gibco #11965118) supplemented with 10% (vol/vol ...
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bioRxiv - Bioengineering 2021Quote: Each sample (n=5) was placed in a 2 mL centrifuge tube containing 320 μL of collagenase II (Gibco, Thermo Fisher Scientific Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... #2) Sterilize the oxygen sensor by submerging the sensor tip in 70% ethanol (Thermo Fisher Scientific, 64-17-5) in an eppendorf tube for 10 sec ...
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bioRxiv - Bioengineering 2021Quote: ... 3% and 5% weight/volume (w/v) MeHA solutions were prepared in Dulbecco’s phosphate buffered saline (DPBS; ThermoFisher, 14190136) with 0.05% w/v Irgacure 2959 (I2959 ...
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bioRxiv - Bioengineering 2020Quote: ... HEK293T cells were seeded on 10-cm plates and transfected 24 h later with 5 μg of Δ19 S_covid-pCMV3 using Lipofectamine 2000 (Invitrogen). Cells’ media were replaced 6 h later to full medium ...
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bioRxiv - Biochemistry 2020Quote: ... at 37 °C with 5 % CO2 with 1 % penicillin/streptomycin (PS, 5000 U.mL-1 and 5000 μg.mL-1respectively; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies).
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bioRxiv - Biochemistry 2021Quote: ... Induction proceeded for 5 h at 37 °C prior to harvesting by centrifugation at 6,000 xg for 15 min (ThermoFisher). Cell pellets were stored at −80 °C until purification.
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bioRxiv - Biochemistry 2020Quote: COS-1 and HEK293T cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2020Quote: ... The staining solutions contained 5 μM CM-H2DCFDA or 3 μM MitoSOX™ (both Thermo Scientific, MA, Waltham, USA) diluted in HEPES/HSA for the detection of intracellular or mitochondrial ROS ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C in humidified atmosphere containing 5% CO2 and routinely passaged by trypsinization with 0.25% trypsin-EDTA (Gibco).
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bioRxiv - Neuroscience 2020Quote: ... the slices were rinsed 3 times in PBS and incubated in PBS with DAPI (5 μg/ml, Invitrogen, #D1306) and the following secondary antibodies at 4°C for 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequently for 1 h in chicken polyclonal antibody against NF200 (RRID: AB_2313552, Aves) diluted 1:1000 in 5% normal goat serum (Cat #PCN5000, ThermoFisher), 0.5% TritonTM X-100 (Cat #9002-93-1 ...
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bioRxiv - Neuroscience 2021Quote: ... Each well was washed 3 times for 5 mins with PBS followed by addition of Goat anti-Rabbit Alexa Fluor®488 (RRID:AB_2534096, ThermoFisher) (1:1000 in blocking buffer ...
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bioRxiv - Immunology 2021Quote: ... islets and tumors were maintained at 35-37°C with flow of 95% O2/5% CO2 saturated RPMI (Gibco). Islets and tumors were imaged on an Olympus FV100MPE microscope or a Leica SP8 DIVE upright multi-photon microscope as previously described (Friedman et al. ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from the differentiated retinal organoids (5 retinal organoids for each condition) using TRIzol reagent (Invitrogen) and was purified using RNeasy kit (QUIAGEN ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were initially incubated for 30 minutes at room temperature with 5 μM Fura-2-AM and 0.005% pluronic acid (ThermoFisher) in HEPES-aCSF solution (125 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Vero E6 and U2OS-ACE2 cell lines were cultured at 37°C in 5% CO2 in DMEM/GlutaMax (Invitrogen) supplemented with 10% heat-inactivated FBS (F9665 ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant protein (dialyzed and lyophilized) was diluted to 5 mg/mL in 200 µL of Dulbecco’s phosphate buffered saline (DPBS) buffer (Gibco; 2.66 mM KCL ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with Triton 0.1% X-100 for 5 minutes at room temperature and stained with Alex Fluor 546 Phalloidin (Invitrogen) 1:2000 for one hour and DAPI (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... and MA104 (ATCC CRL-2378.1) cells were maintained at 37°C in a fully humidified atmosphere with 5% CO2 in DMEM (Invitrogen) and M199 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated EVs in PBS (5 - 10 μg protein) were acidified with trichloroacetic acid (TCA; Fisher Scientific, Cat #A322-100) (10% vol:vol final ...
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bioRxiv - Cancer Biology 2020Quote: All DLBCL cell lines were cultured at 37°C in 5% CO2-containing humidified incubators using Advanced RPMI (Gibco) containing 5% fetal bovine serum (Hyclone or Seradigm ...
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Cell Biology 2021Quote: ... amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher) with the primers shown in Supp ...
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bioRxiv - Cell Biology 2021Quote: ... cells and cervical adenocarcinoma epithelial (HeLa) cells were cultured at 37°C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Neuroscience 2020Quote: ... Cell cultures were maintained in an incubator with 5% CO2 (Forma Series II Water Jacket CO2 Incubator, Thermo Scientific) at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... The marrow was flushed out of the bones via 2 - 5 mL 4 °C DPBS (Dulbecco’s Phosphate-Buffered Saline; Cat. No. 21-031-CV; Gibco) injection ...
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bioRxiv - Biochemistry 2021Quote: ... After loading onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, Thermo Scientific), peptides were separated on a reversed-phase Easy Spray C18 column (50 cm x 75 μm ID ...
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bioRxiv - Biochemistry 2021Quote: ... were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was diluted to 50 µM and labelled with a 5:1 molar excess of fluorophore:protein with either AlexaFluor 488 C5- Maleimide (Invitrogen) (AF488) ...
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bioRxiv - Biochemistry 2020Quote: StNFeoB proteins (2-5 µM each) were incubated with a range of substrate (GTP for molecular biology, Thermo Scientific) concentrations (25 µM to 5000 µM) ...
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bioRxiv - Cell Biology 2021Quote: ... ALI-differentiation medium was removed and refreshed with ALI-differentiation medium supplemented with DAPT (5 mM, Fisher Scientific #15467109) for 14-18 days ...
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bioRxiv - Cell Biology 2021Quote: ... 5% of total RNA was DNase-treated in 50 µl reactions with TURBO DNase (Thermo Fisher Scientific, Cat# AM1907) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... MSn fragmentation data of 5 was acquired using an ESI-LTQ Orbitrap XL (Thermo Fisher Scientific GmbH, Bremen, Germany) MS spectrometer.
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bioRxiv - Systems Biology 2021Quote: ... A 1:5 dilution of this protein solution was quantified using a Pierce BCA protein assay kit (Thermo scientific). For analysis of the relative composition of amino acids ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of total RNA was treated with TURBO DNA-free™Kit (Invitrogen, Thermo Fisher Scientific, MA, USA) to remove any potential genomic DNA contaminants ...
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bioRxiv - Microbiology 2020Quote: ... African green monkey kidney epithelial Vero E6 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 5% fetal bovine serum (FBS; HyClone Laboratories, South Logan, UT) and 1% antibiotic/ streptomycin (Gibco). Human lung adenocarcinoma epithelial Calu-3 2B4 cells were maintained in a high-glucose DMEM supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were blocked with 5% (w/v) non-fat milk in TBS- Tween-20 (TBST) buffer (Thermo Scientific) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Microbiology 2020Quote: ... ATCC) monolayers in Dulbecco modified Eagle medium (DMEM) supplemented with 5% heat-inactivated fetal bovine serum (FBS) (Gibco/Invitrogen), 100 U/ml penicillin ...
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bioRxiv - Microbiology 2020Quote: ... a DNA fragment comprising the entire coding region of kpfR plus approximately 550 pb of 3’ and 5’ flanking regions (Table S4 in the supplemental material) was inserted on pCR2.1-TOPO vector (Invitrogen) previously cloned with erythromycin-resistance gene ...
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bioRxiv - Plant Biology 2020Quote: ... two-day-old etiolated seedlings were incubated in half strength OS medium containing 5 µM FM4-64 (Thermo Fisher) for 1 h with blue light treatment ...