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Citations for Thermo Fisher :
8301 - 8350 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The slices were then washed 3 x 5 min in 0.5% PBST followed by incubation in secondary antibodies (goat anti-mouse Alexa Fluor 405 1:500, Invitrogen A31553, RRID:AB_221604; goat anti-rabbit Alexa Fluor 488 1:500, Invitrogen A11034 ...
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bioRxiv - Biophysics 2024Quote: ... Mouse embryonic stem cells were expanded with 1:1 DMEM/F12 (ThermoFischer 31331028) and neurobasal medium (Gibco 21103049) supplemented with 1x N2 (Gibco 17502048) ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 488 anti-rabbit (1:1000, green) and 568 anti-mouse (1:1000, red) (both Invitrogen; Table 4) secondary antibodies were also diluted in 1% BSA and 10 % goat serum in TBS and incubated in the dark for 45 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 150mM NaCl and 0.1% Tween20) for 1 hour and incubated with mouse anti-HA (Invitrogen #26183, 1:500) in blocking solution overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Immunology 2023Quote: ... This was followed by another wash step and the addition of Goat anti-Mouse IgG (1:10,000) or IgM (1:5,000) as a secondary antibody (ThermoFisher). The plates were then incubated at room temperature for one hour ...
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bioRxiv - Immunology 2023Quote: ... Isolated CD8 T cells were activated via 1:1 culture with Dynabeads Mouse T-Activator CD3/CD28 (Invitrogen) in T cell media + BME in 96 well U-bottom plates for 48 hours ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Neuroscience 2022Quote: ... a cell surface biotinylation assay was performed using a commercially available kit (Pierce Cell Surface Protein Isolation Kit, Thermo Fisher Scientific, catalog #89881). Eluates were subjected to immunoblot analysis using a Plexin-A4 antibody (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2023Quote: The immunoprecipitation of endogenous PSY was performed using P-PER® Plant Protein Extraction Kit and Pierce Co-Immunoprecipitation kit (both from Thermo Fisher Scientific) following manufacturer’s instructions and is summarised below.
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bioRxiv - Neuroscience 2024Quote: ... The total protein content from the crude membrane extracts was determined using the bicinchoninic acid assay 46 with a commercially available kit (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Immunology 2019Quote: All the receptors used in this study were either cloned into bicistronic pEFIN3 vector (Euroscreen) for stable expression or pcDNA3 vector (Life Technologies) for transient expression (S1 Table) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Immunology 2020Quote: The Fab fragments for anti-SARS-CoV-2 antibodies ION_300 and ION_360 and SARS-CoV-2 receptor binding domain (RBD) were expressed in Expi293F™ cells (Thermo Fisher, A14527). The antibody:RBD complexes were mixed and co-purified by size exclusion chromatography in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Neuroscience 2020Quote: Cells were incubated with anti-human Fc receptor (0,005 µg/ml eBioscience, 14-9161-73) for 10 min in Medium A without phenol red (HBSS (Gibco, 14170-053) containing 15 mM HEPES (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... the P2X2 receptor was conditionally expressed into SST+ interneurons and selectively stimulated by laser uncaging of DMNPE-caged ATP (100μM, Life Technologies, UK). LSPS was performed using an ultraviolet (UV ...
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bioRxiv - Cancer Biology 2020Quote: ... SSOs specific to insulin receptor pre-mRNA were transfected in cells with either Lipofectamine 2000 (Catalog Number 11668030) from Life Technologies.
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Plant Biology 2023Quote: ... coding sequences of receptor and effector genes with or without stop codons were either synthesized as pDONR221 entry clones from GeneArt (Thermo Scientific), or were published previously (Saur et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Neuroscience 2023Quote: ... A DNA fragment containing the nucleotide sequence of the membrane-bound EGFP link via a T2A cleaving site to the TVATC66 receptor Field 69 coding sequence was designed in an inverted orientation and ordered via GeneArts (ThermoFisher Scientific). This DNA sequence was subsequently amplified by Q5 Hot Start High Fidelity DNA polymerase-based PCR (New England Bioscience ...
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bioRxiv - Cell Biology 2019Quote: ... For the negative control primary antibodies mouse anti-plasmepsin V (From D. Goldberg, 1:1) and rabbit anti-GFP A-6455 (Invitrogen, 1:200) were used.
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The blot was then exposed to secondary antibodies (dilution 1:10,000 for Tuj-1 and 1:5000 for pan-Ago, Goat Anti-Mouse, Thermofisher Scientific, Catalog# A11030) for 3 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... Germany) has been conjugated to AF546 using the AlexaFluor® 546 protein labeling kit (Molecular probes) and anti-NF200 has been conjugated to AF647 using the AlexaFluor® 647 protein labeling kit (Molecular probes).
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration in the supernatant was quantified using the Pierce BCA Assay Kit (Thermo Scientific #23227). For immunoblotting ...
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bioRxiv - Cell Biology 2019Quote: ... 0.5% SDS) and immunoprecipitation carried out using the Dynabeads Protein G Immunoprecipitation Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The blotted proteins were developed using an enhanced chemiluminescence kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The protein content was determined using the bicinchoninic acid assay kit (Pierce™, Thermo Fisher Scientific) to adjust the protein concentration for immunoblotting and to calculate relative proinsulin and insulin content.
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bioRxiv - Genetics 2021Quote: ... clarified by centrifugation for 10 minutes and quantified using a microBCA Protein Assay Kit (Thermo Scientific). For membrane based western blot ...
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bioRxiv - Cell Biology 2022Quote: ... cells were prepared using the Subcellular Protein Fractionation Kit for Cultured Cells (78840 from ThermoFisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein levels were measured using the Pierce™ BCA Assay Kit (Thermo Fisher cat# 23225).
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bioRxiv - Pathology 2019Quote: ... spectrophotometer with a 562 nm filter using the Pierce™ BCA Protein Assay kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... Total protein was determined using Pierce BCA assay kit (Thermo Fisher Scientific Inc., Waltham, MA, USA) per the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... protein concentration was determined using the enhanced protocol of the Pierce BCA assay kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Protein bands were detected using chemiluminescence according to the manufacturer’s instructions (SuperSignal West Pico kit (ThermoFisher)).
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bioRxiv - Biophysics 2019Quote: ... The antibody was labeled by TexasRed (Texas Red™-X Protein Labeling Kit, ThermoFisher, CA, USA). Labeling efficiency of the antibody was determined as 1.52 TexasRed/antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Co-immunoprecipitation was carried out using Pierce Protein A/G magnetic beads kit (Thermo Scientific, 88802) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Pierce Bicinchoninic acid (BCA) and MicroBCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). Quant-iT PicoGreen dsDNA assay kit was procured from Molecular Probes ...
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bioRxiv - Bioengineering 2020Quote: ... The amount of collagen was determined using Pierce™ BCA Protein Assay Kit (Fisher Scientific, PA). Standard curve was constructed using known concentrations of collagen type-1.
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bioRxiv - Cell Biology 2020Quote: ... Cleared lysates were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Equal amounts of proteins were resolved on 6 or 10% SDS-PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... the concentrations of each samples were measured using BCA protein assay kit (#23225, Thermo Fisher Scientific). Equal volumes (30 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were equalized using the Pierce BSA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA), per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of the lysates was measured with the BCA protein assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... The concentration of conjugated oligo DNA and antibody with BCA Protein Assay Kit (Thermo Scientific 23225) were quantified using Nanodrop.