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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Bradford assay was performed for determining the protein concentration of each sample using Pierce BCA protein assay kit (Thermo Scientific, Pittsburgh, PA, USA, Ref.23225) and a Tecan Infinite 200 plate reader (Tecan Group Ltd. ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was collected and used to quantify total extracted protein using a BCA protein quantification kit compatible with denaturing agents such as SDS and ß-mercaptoethanol (Thermo Fisher Scientific, Whatham, USA). Fifty micrograms of protein for each treatment was loaded onto a (4-20)% acrylamide SDS-PAGE denaturing protein gel (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... D1-3 and D4-5 proteins were fluorescently labelled with Alexa Fluor™ 594 or 488 Microscale Protein Labeling Kits (Invitrogen™, A30008 or A30006) as described [15] ...
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bioRxiv - Biophysics 2023Quote: ... Total protein concentrations of tissue and cancer cell samples were determined using the Pierce BCA Protein Assay Kit (#RG235622, Thermo Fisher Scientific, Waltham, MA, USA). Further steps were performed as described for protein isolation from mouse organ tissues.
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
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Identification of ABHD6 as a regulator of lysophosphatidylserines in the mammalian liver and kidneysbioRxiv - Biochemistry 2024Quote: ... The protein concentrations of the membrane and soluble proteomic fractions were measured using the Pierce BCA Protein Assay Kits (Thermo Fisher Scientific, catalog no: 23225).
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bioRxiv - Molecular Biology 2024Quote: Nascent protein synthesis was analyzed in HPMs by using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen™, cat #C10428). Click-iT® HPG (L-homopropargylglycine ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were excluded using ThermoFisher LIVE/DEAD Fixable Aqua Dead Cell Stain and binding to Fc receptors was blocked using CD16/CD32 (clone 93, eBioscience/ThermoFisher). Absolute cell numbers were calculated using counting beads (123count eBeads ...
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bioRxiv - Immunology 2019Quote: ... and cells stably expressing the receptors of interest were selected and cultured in the presence of 800 µg/ml G418 (Invitrogen). The human embryonic kidney cell line HEK293T (CRL-3216 ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... For knockdown experiments MIN6 cells were resuspended with 25 nM antiGABA-B1 receptor or control siRNA (Dharmacon, siGENOME) pre-mixed with 1.6 μl/ml Lipofectamine RNAiMAX (Life technologies) in Opti-MEM I medium ...
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bioRxiv - Cell Biology 2020Quote: Parent CHO-K1 and CHO-K1 cells stably expressing extracellular ACP-tagged human insulin receptors were maintained at 37°C with 5% CO2 in HAM’s F-12 medium (Thermo Fisher) supplemented 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2022Quote: ... cells and baby hamster kidney 21 cells expressing the MHV receptor (BHK-R)(47) were maintained in DMEM containing 10% FBS (Invitrogen), 100 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... or with an antibody against the nicotinic acetylcholine receptor ɑ7 (nAChr ɑ7; Alomone Labs) followed by an Alexa594 goat-anti-rabbit conjugate (Invitrogen). Nuclear counterstain with Hoechst 33342 (Sigma ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Biochemistry 2020Quote: ... , S1 subunit mutants and the extracellular domain of ACE2 receptor (GenBank NM_021804.3) were Baculovirus-free produced in High Five cells (Thermo Fisher Scientific) by transient transfection as previously described in Bleckmann et al ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: CHO cell lines overexpressing FPR2 and FPR1 receptors were generated in-house and propagated in Ham’s F12 medium (Gibco Biosciences) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... and 20uL was added to each well of a 96 well TaqMan Array plate with chemokine/chemokine receptor primers (ThermoFisher, Mouse Chemokines & Receptors Array plate ...
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bioRxiv - Immunology 2021Quote: ... against Fcγ receptor RI (CD64)-FITC, FcγRIII (CD16)-PE, CD14-PerCP (Becton Dickinson, USA) and FcγRII (CD32)-ACP (Life technologies, USA) and isotype and fluorochrome matched negative control mAbs (BD ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293A cells expressing dopamine receptor sensors or other constructs were prepared on cover-glass-bottom dishes coated with 10 μg/ml of fibronectin (Gibco). Various concentration of dopamine or other ligands were added to the cells ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then processed for real-time polymerase chain reaction (RT-PCR) to assess the expression of the oxytocin receptor (primers and probe assay ID: Rn00564446_g1, purchased from Life Technologies). In particular ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Microbiology 2023Quote: ... 293T-hACE-2 (BEI resources, NIH, Catalog No. NR-52511) cells expressing the ACE2 receptors were cultured in DMEM (Gibco) supplemented with 5% FBS (Fetal Bovine Serum) ...
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bioRxiv - Molecular Biology 2023Quote: ... fluorescently labeled GST-antibody (final concentration of 25 nM) and Nuclear Receptor Buffer F (A15668, A11131, PV4547, Thermo Fisher Scientific) supplemented with 1 μM compound or solvent only (DMSO ...
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature for all target proteins or with clear milk blocking buffer for β-actin protein (Pierce, Thermo Scientific). We used the following LARP1 (Bethyl Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody-bound NLGNs were incubated for 1 hour with 20 µL of protein G beads (Dyna-beads Protein G, Thermo Fisher Scientific) precipitated and washed 4 times with lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... microsphere mixtures were successively incubated with serum samples (1:400) biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific), and Streptavidin-R-Phycoerythrin (4 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: Each extracellular protein fraction (#1-15) collected by UF-SEC was collected and protein concentration determined by Micro BCA assay (Thermo Fisher Scientific) as according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... the membrane preparations were solubilized with 1% DDM and the total protein content was measured using Pierce™ 660 nm Protein Assay Reagent (Thermo Scientific) against the BSA standard curve (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: FITC and Alexa Fluor 647 labelling of purified proteins was done by using FluoroTag FITC Conjugation Kit (MilliporeSigma, FITC1-1KT) and Alexa Fluor™ 647 Antibody Labeling kit (Invitrogen, A20186), respectively ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... sections were transferred to secondary antibody cocktail (Alexa Fluor 568 donkey anti mouse, 1:500; Alexa Fluor 488 donkey anti rabbit, 1:500; NeuroTrace Nissl 640/660, 1:500; all from Life Technologies) for 2-hour incubation at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Monolayers were incubated with rabbit anti-mouse zonula occludens-1 (ZO-1) antibody (1:50; cat# 61-7300, Invitrogen, Camarillo, CA) or rabbit anti-mouse occludin antibody (1:200 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 h with secondary antibodies (anti-Mouse IgG 488, 1:1000 Invitrogen, #A11029; anti-rabbit IgG 488, 1:1000, Invitrogen, #A11034), followed by DAPI staining (5 μg/ml).
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bioRxiv - Microbiology 2020Quote: ... The membranes were washed and incubated with an HRP-conjugated secondary antibody (anti-mouse, 1:5,000, 31430; anti-rabbit, 1:5,000, 31460; anti-goat, 1:5,000, R-21459, Invitrogen, Grand Island, NY, USA). The membranes were then washed again ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Tissue sections were incubated with rat anti-mouse primary monoclonal mast cell protease-1 (MCPT-1) Ab (1:100, clone RF6.1; Thermo Fisher Scientific), followed by secondary staining with biotinylated rabbit anti-rat IgG (Abcam) ...
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bioRxiv - Genomics 2023Quote: ... rabbit anti-H3K9me3 (1:200), rabbit anti-H3K27me3 (1:1000) (Frapporti et al., 2019) and mouse anti-FLAG (1:200) (Thermo Fisher Scientific Cat#MA1-91878 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10, anti-mouse Minus-probe, 1:10, and anti-chicken Alexa Plus 647-secondary antibody, 1:1,000, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... were diluted in 50 mM carbonate buffer to a concentration of 1 μg/ml and coated at 50 ng/well on 96-well ELISA plates (MaxiSorp Nunc, Thermo Fisher Scientific K.K., Japan) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... α-SC35 (described previously98, Mouse 1:1000), α-NIN (Institut curie, Human 1:200), anti-Arl13b (proteintech, Rabbit, 1:4500) α-Rabbit Alexa594 (ThermoFisher, 1:400), α-Mouse IgG1 Alexa488 (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... containing LCMV peptides (1:1000, Peptide 2.0 Inc.) and Protein Transport Inhibitor Cocktail (1:500, Thermo Fisher Scientific). Cells were incubated for 4 hours at 37°C and then surface antigens were stained as described above ...