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Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... rabbit anti-phospho-S6 ribosomal protein (Serine244/247) (1:150, Invitrogen, #44-923G), rabbit anti-MS4A1/CD20 (1:250 for immunostaining ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were separated on 1-mm-thick 16% Tricine gels (Life Technologies) for 2 h at 120 V ...
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bioRxiv - Cell Biology 2023Quote: ... Streptavidin-HRP (peroxidase-conjugated streptavidin biotin-binding protein, Thermo Scientific, 21130, 1:10000).
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bioRxiv - Biophysics 2023Quote: ... protein digests were mixed with 1 mL HisPur Ni-NTA resin (ThermoFisher Scientific) and placed on a rotating mixer maintained at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of native marker (Native Marker unstained protein standard, LC0725, Life Technologies) diluted 400 x in the GF buffer was used for mass/contrast calibration that was monitored during 60 s using the AcquireMP software (Refeyn Ltd ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were reduced by adding 1 mM DTT (Thermo Fisher Scientific, A39255) and incubating for 20 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... Protein (8 µM) and cytidine triphosphate (CTP; Thermo scientific, cat R0451, 1 mM) were incubated in the reaction buffer (20 mM Tris.HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... and SARS-CoV-2 nucleocapsid protein (N, clone E16C, Fisher Scientific, 1:25) were diluted in blocking solution and then added and incubated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad ...
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bioRxiv - Neuroscience 2021Quote: ... rRNA were depleted from 1 μg of total RNA using the NEBNext rRNA depletion kit (Huma/Mouse) and ribo-depleted RNA was quantified by Quant-iT ™ RiboGreen ™ RNA Assay Kit (Invitrogen). Using a reverse transcriptase and random primers ...
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bioRxiv - Genetics 2023Quote: ... ADH enzyme activity (units: ΔAbs340nm min−1 mL−1) and total protein (units: mg mL−1; Pierce BCA Assay, Thermo Fisher Scientific) of each sample were measured 3 times in technical replicates ...
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bioRxiv - Molecular Biology 2019Quote: ... quickly washed with PBS+ before incubating with 70 µl of secondary antibody mix (Goat anti-mouse Alexa Fluor® 488 or Goat anti-mouse Alexa Fluor® 594 1/400 in PBS+, Invitrogen) for 1 h at RT in a moist chamber ...
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bioRxiv - Physiology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine, Invitrogen, T2762) for 45 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies used were goat anti-mouse IgG2b 594 and goat anti-mouse IgG1 647 (all from Molecular Probes, 1:500). After several washes in PBS-T ...
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Biochemistry 2020Quote: Due to the lack of aromatic amino acids within the small protein His6-sP26 the Pierce™ BCA protein assay kit (Thermo Fisher Scientific™, Waltham, USA) was used to determine the concentration of all proteins and was performed according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the resulting lysate supernatants were measured using the BCA assay (Pierce™ BCA Protein Assay Kit, Thermo Fisher Scientific, Ref: 23225, Lot: UE284353) and adjusted to 2.0 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: The cells were lysed by radioimmunoprecipitation assay (RIPA) buffer and protein concentrations of lysate were determined using a BCA Protein Assay Kit (#23225, Thermo Fisher Scientific Inc., Waltham, MA, USA). Equivalent amounts of proteins were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Microbiology 2022Quote: ... The purity of recombinant proteins was analyzed by 10% Tris/Tricine SDS-PAGE and silver staining techniques and the protein concentrations were determined by employing the Pierce™ BCA protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2023Quote: ... Samples were stored at −80 °C or directly used for the quantification of total protein concentrations with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, United States). Thereafter ...
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bioRxiv - Neuroscience 2024Quote: Total protein levels were quantified in tissue homogenates in triplicates using the Pierce Microplate BCA Protein Assay Kit (Thermo Scientific Pierce Protein Biology, Thermo Fisher Scientific, Waltham, MA, USA). Individual cytokine levels are reported as pg/mg of protein in each sample.
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein from respective groups were pooled together and precleared on ice using normal control mouse IgG (Life Technologies) for 30 minutes followed by incubating with A/G PLUS-Agarose beads (Santa Cruz ...
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bioRxiv - Immunology 2022Quote: ... Each of these medium samples was then applied to NA Protein G Spin Columns to purify the CspZ-YA mouse monoclonal IgG (ThermoFisher Scientific) and then quantitated as described in the vendor’s manual.
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bioRxiv - Biophysics 2024Quote: ... in PBS and incubated over night at 4 °C with SARS-CoV-2 Spike Protein S2 mouse monoclonal antibody 1A9 (Thermo Fisher) and TSG101 rabbit polyclonal antibody 14497-1-AP (Proteintech ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were incubated overnight at 4 °C with mouse anti-ee antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific( ...
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bioRxiv - Cancer Biology 2023Quote: ... The horseradish peroxidase (HRP)-conjugated Protein A (#101023) and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) for immunoblotting were from Thermo Fisher. Antibodies used for immunofluorescence studies were as follows ...
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bioRxiv - Plant Biology 2021Quote: ... The activity of the mitochondrial proteins (1-25 μg protein) were assayed spectrophotometrically using a Multiskan™ GO Microplate Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Molecular Biology 2022Quote: ... Wells were coated with 1 μg Gfp for anti-GfpNB or 1 μg commercially available SARS-CoV-2 Spike-RBD-domain protein for SARS-CoV-2 nanobody-Cts1-fusion proteins (Invitrogen, Waltham Massachusetts, USA). Recombinant Gfp was produced in E ...
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bioRxiv - Physiology 2024Quote: ... 1 x 5 min in TBS) prior to total protein staining using SYPRO Ruby Protein Blot Stain (Invitrogen Canada Inc, Burlington, ON, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... with 1 mL of 1 μg/mL of anti-mouse IgG secondary antibody conjugated with AlexaFluor 488 (Invitrogen). After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... at 37°C for 1 hour and then with anti-mouse IgG AlexaFluor 488 (Invitrogen A11029, 1:200) and anti-rat IgG AlexaFluor 555 (Invitrogen A21094 ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 1 h with secondary antibodies (Alexa Fluor 568 anti-mouse, 1:100, A-11031, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used for IF were monoclonal mouse Prox-1 (1:50, P21936, ThermoFisher Scientific, Massachusetts, USA), monoclonal rabbit Lyve-1 (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated for 1 hour with Alexa Fluor 647–conjugated goat anti–mouse antibody (1:500, Molecular Probes) and Hoechst 33258 (1:1000) ...
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bioRxiv - Neuroscience 2019Quote: ... Rabbit anti-GFP tagged FITC (1:500) and mouse anti-Gal4 (1:200) were acquired from Thermo Scientific. Dissected larvae were mounted in Vectashield (Vector Labs ...
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bioRxiv - Neuroscience 2021Quote: ... and a mouse monoclonal antibody against human KMO (# 60029-1-Ig 1:1000, Thermo Fisher Scientific, Massachusetts, EUA). The membranes were washed and incubated for 1 h at room temperature with an HRP-conjugated secondary antibody (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight with primary antibody (Invitrogen rabbit anti-GFP, 1:1,000; Invitrogen mouse anti-HA, 1:5,000). HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... S2 using the additional primary antibodies mouse anti-α-actinin-1 (1:200, Thermo Scientific clone BM 75.2) and mouse anti-vinculin (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... to visualize infection ii) mouse monoclonal anti-ZO-1-1A12 (1:500, Thermo Fisher cat no. 33-9100) and goat anti-mouse AlexaFluor 647 to visualize tight junctions ...
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bioRxiv - Cell Biology 2020Quote: ... acetylated α-tubulin (Cell Signaling, #5335T Rabbit at 1:2000 and Invitrogen, #32-2700 Mouse at 1:2000), TOM20 (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Blots were visualised with HRP-conjugated goat anti-rabbit antibody (1:10,000) or HRP-conjugated goat anti-mouse (1:10,000) (ThermoFisher) and developed using enhanced chemiluminescence (ECL ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 hr incubation with secondary antibodies anti-mouse Alexa Fluor 594 (1:500, #A11062, Life Technologies) and anti-rat Alexa Fluor 488 (1:500 ...