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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for Mouse Receptor Interacting Serine threonine Protein Kinase 1 RIPK1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... The protein concentration of Matrigel was adjusted by dilution with 1:1 DMEM:F12 medium (Life Technologies, Carlsbad, CA). The molecular crowders ...
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bioRxiv - Developmental Biology 2019Quote: ... Chromatin was pre-cleared with 5 or 10 µl of 1:1 protein A:G Dynabeads (Life Technologies #10015D) and immunoprecipitated with H3K4me1 (Abcam #ab8895) ...
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bioRxiv - Cell Biology 2022Quote: Protein was added to 1× NuPAGE LDS Sample Buffer and 1× NuPAGE Reducing Agent (both Thermo Fisher Scientific) in water and incubated at 75°C for 10 minutes for protein denaturation ...
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bioRxiv - Developmental Biology 2021Quote: ... Mouse cell nucleocytoplasmic fractionation and RNA isolation was performed with the PARIS kit (ThermoFisher AM1921) according to the manufacturer’s instructions plus an additional ethanol precipitation step and rehydrated in nuclease-free water ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then lineage-depleted using Dynabeads Untouched Mouse CD4 Cells Kit (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse brain samples were fractionated using the Pierce Subcellular fractionation kit for tissues (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from mouse bladders using TRIzol Reagent and PureLink RNA Mini Kit (Invitrogen), according to manufacturers’ instructions ...
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bioRxiv - Physiology 2021Quote: ... RNA samples were prepared from the mouse tissues using the PureLink RNA Mini Kit (Invitrogen by ThermoFisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Total RNA was isolated from mouse liver using PureLink RNA mini KIT (Thermo Fisher Scientific). cDNA synthesis was carried out using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted from ferret blood using the Mouse RiboPure-Blood RNA Isolation Kit (Ambion). Both extraction methods followed the manufacturer’s protocols and incorporated a DNase treatment (QIAGEN ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells are enriched using MagniSort Mouse CD4+ T cell Enrichment Kit (Thermofisher scientific) and seeded at a density of 1,5.106 cells/ml with ½ fresh medium and ½ culture medium supplemented with IL-2 (10ng/ml) ...
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bioRxiv - Immunology 2019Quote: Fab preparation was performed using the Mouse IgG1 Fab preparation micro kit (Thermo Scientific #44680) and CD38 ...
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bioRxiv - Cancer Biology 2024Quote: siRNA for mouse-IGF1R was constructed in vitro using the Silencer siRNA Construction Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cochlear RNA was extracted from C57BL/6J mouse with Micro Scale RNA isolation Kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and other mediators by 22-plex ProcartaPlex mouse basic kit (Thermo Fisher Scientific, Waltham, USA) on a Luminex-200 instrument (BioRad ...
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bioRxiv - Biophysics 2024Quote: ... CD4+ cells were isolated using DynabeadsTM UntouchedTM Mouse CD4 Cell Kit (Cat #: 11416D, ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: A custom-made mouse Cytokine 26-Plex-assay kit was designed and purchased from Invitrogen. The kit included specific quantification of mouse Eotaxin ...
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bioRxiv - Plant Biology 2019Quote: ... the coding region of CRK2 or kinase-dead mutants (K353E or D450N) were recombined into pENTR/D-TOPO vector (Invitrogen). pDONRP4P1R/zeo-CRK2pro ...
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bioRxiv - Biochemistry 2020Quote: ... De-phosphorylated tsRNA or synthetic RNA (scrambled) were 5’-thiolated by incubation with 0.5 U/µL polynucleotide kinase (ThermoFisher Scientific) in the presence of 0.5 mM ATPγS (SIGMA ...
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bioRxiv - Microbiology 2021Quote: ... and were maintained and virally transduced in F-media with mitomycin C treated 3T3 cells and Rho Kinase inhibitor (Y-27632, ThermoFisher) as previously described (40).
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bioRxiv - Plant Biology 2021Quote: ... the coding sequence of the kinase domain of CRK10 was amplified with SalI and NotI restriction sites and cloned into pENTR1A Dual Selection Vector (Invitrogen) prior to recombination with pDEST17 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were stripped and developed with antibodies against phosphoglycerate kinase (Pgk1; Life Technologies, Grand Island, NY, USA, cat: 459250) as an internal loading control.
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bioRxiv - Biochemistry 2022Quote: ... recombinant human RPA or RPA-variants (2 μM) were incubated with 250 nM of recombinant Aurora B kinase (Invitrogen Inc.) in kinase reaction buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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bioRxiv - Neuroscience 2022Quote: ... Kinase assay was then performed by incubating with 0.5mM ATP and Halt™ Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher) for 60min at 30°C on a shaking heat block ...
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bioRxiv - Biophysics 2024Quote: ... The linear PCR product was phosphorylated with T4 Polynucleotide kinase and circularized using T4 DNA-ligase according to manufacturer’s instructions (Thermo Scientific). All constructs were verified by Sanger Sequencing.
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bioRxiv - Biochemistry 2023Quote: Kinase reactions (10 μL) were briefly subjected to SDS-PAGE for 5 min using the Bolt Bis-Tris system (Invitrogen). The gel was stained with 25 mL of InstantBlue Coomassie Protein Stain (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with 100nM non-targeting control or individual kinase targeting On-TARGETplus SMARTPool siRNAs (horizon; Table S6) including four unique target sequences and 5µl RNAiMAX (ThermoFisher #13778150) in Opti-MEM (ThermoFisher #31985088 ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Plant Biology 2024Quote: ... The probe was 5’-end labeled with [γ-32P]-ATP and T4 Polynucleotide Kinase (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to a kinase buffer (50 mM Tris pH 8.0, and 100 mM NaCl) using Zeba Spin Desalting columns (ThermoFisher Scientific). Proteins were separated by SDS-PAGE and stained with Coomassie blue ...
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bioRxiv - Biophysics 2021Quote: ... 10 μl protein (0.15 μg μl−1) with 10x SYPRO Orange dye (Invitrogen) end concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Protein samples were detected with 1:2,000 diluted anti-GFP (A6455; Thermo Scientific) and 1:1,000 diluted anti-RFP (AB233 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) followed by an incubation with antibody/protein A Dynabeads (Invitrogen) complex at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1-3 mg/ml and Dynabeads® Protein G (Thermo Fisher Scientific) according to the manufacturer’s instruction.
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bioRxiv - Bioengineering 2021Quote: ... Recombinant protein expression was triggered using 0.5mM isopropyl β-D-1-thiogalactopyranoside (ThermoFisher) and incubated for 18 h in an orbital shaker at 18 °C ...
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bioRxiv - Neuroscience 2021Quote: ... along with a 1:1000 dilution of Sypro Orange Protein Gel Stain (Invitrogen) and a 1:10 dilution of 200 μM Tris pH 7.5 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 unit of chromatin was immunoprecipitated by protein A magnetic beads (Dynabeads, Invitrogen) after incubation with antibodies specific for HDAC1 (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... green fluorescent protein (GFP, conjugated with Alexa 488; rabbit, 1:1000, A21311, Invitrogen; or unconjugated ...
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bioRxiv - Biochemistry 2022Quote: ... Protein cleavage was carried out by addition of 1 μL TEV protease (Invitrogen) to cleave the SMC compartment and 1 μL HRV 3C protease (Pierce ...
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bioRxiv - Biochemistry 2022Quote: ... Protein cleavage was carried out by addition of 1 μl TEV protease (Invitrogen) to cleave the SMC compartment and 1 μl HRV 3C protease (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-green fluorescent protein (GFP) (1:500, #A11122, Life Technologies, Waltham, Massachusetts) and rat anti-N-Cadherin (1:50 ...
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bioRxiv - Molecular Biology 2019Quote: 30 μg of aggregated proteins in 1 x LDS loading buffer (Life Technologies) supplemented with 100 mM DTT were separated on 4-12% Bis-Tris gels in MOPS running buffer ...
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bioRxiv - Bioengineering 2020Quote: ... and 1% (v/v) PFHM-II protein-free hybridoma medium (Thermo Fisher Scientific). On day 4 of differentiation ...
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bioRxiv - Genomics 2021Quote: ... Fifteen µl of Pre-blocked (with 1% BSA) Protein G Dynabeads (10004D, Invitrogen) was added to the tubes and rotated for 4h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... Associated proteins were removed by digesting with 1 mg/ml proteinase K (Invitrogen) for 20 minutes at 37°C in PK buffer (100 mM Tris-HCl pH7.4 ...
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bioRxiv - Microbiology 2024Quote: ... NS1 proteins were detected using DENV2 NS1 polyclonal antibody (1:1,000 ThermoFisher Scientific) with overnight incubation at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and digested with sequencing grade trypsin (∼1:100 enzyme/protein ratio; Thermo Fisher) at 37