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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... qPCR was conducted using QuantStudio 3 Real-Time PCR System (Applied Biosystems) with Power SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and nuclear dye (TO-PRO-3 Iodide, 1:400, Thermo Fisher T3605) were added to the blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... and a 1:400 dilution of TO-PRO-3 (Thermo Fisher T3605) for 24 hr at 4°C in the dark ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were run on 3-8% Tris-Acetate gels (Thermo Fisher, 12095655). Prestained protein ladder (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and run on a QuantStudio 3 Real Time PCR System (Applied Biosystems). mRNA expression levels were normalized to the expression level of chicken 18S ribosome (Himmels et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... The membrane was blocked with 3% BSA in TBST (Pierce, Thermo Scientific) with 0.1% Tween-20 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were pre-complexed with 3 µg lipofectamine 2000 (Life Technologies 11668019) in 200 µl Opti-MEM (Life Technologies 51985091 ...
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bioRxiv - Genomics 2020Quote: ... and 3 mg/mL collagenase type I (17100-017, Gibco, Carlsbad, CA) at 37 °C and 60 rpm for 16 h ...
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bioRxiv - Genomics 2020Quote: ... The sample was run on a 3-8% Tris Acetate Gel (Invitrogen) for 1 hour at 150 V ...
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bioRxiv - Immunology 2020Quote: ... 5’/VIC/CCTTGGGTTCTTGGGA/3’/MGB (Thermo Fisher Scientific; (Bruner et al., 2016)) ...
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bioRxiv - Immunology 2020Quote: ... 5’/NED/AAGCCAGGAATGGATGGCC/3’/MGB (Thermo Fisher Scientific; (Schmid et al., 2010)).
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bioRxiv - Physiology 2020Quote: ... using an Applied Biosystems QuantStudio 3 real-time PCR system (ThermoFisher Scientific) as we previously described 66 ...
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bioRxiv - Biochemistry 2020Quote: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Thermo Fisher Scientific) was added to cells at a final concentration of 2mM and incubated at 37°C in 5%CO2 for 1 h ...
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bioRxiv - Bioengineering 2020Quote: ... Thermocycling was performed in a qRT-PCR machine (Quantstudio 3, Applied Biosystems) with the following cycling conditions ...
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bioRxiv - Developmental Biology 2021Quote: ... and rabbit anti-activated Caspase-3 (Fisher Scientific/BD, BDB559565, 1:500). Antibody used for fluorescent in situ hybridization was mouse anti-Dig (Jackson ImmunoResearch 200-002-156 ...
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bioRxiv - Cancer Biology 2021Quote: ... Reactions were performed using a Quant Studio 3 PCR machine (Thermo Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... CellEvent™ Caspase-3/7 Green live staining detection reagent (Thermofisher Scientific) at 2 μM was prepared and added to the epithelial and vascular channels in order to visualize an apoptotic T-cell killing response ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was then performed on a QuantStudio 3 machine (Thermo Fisher Scientific) using SYBR Premix Ex Taq II (TaKaRa) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with urethane (3 g/kg body weight; Fisher Scientific) and transcardially perfused for one minute with gravity-fed ice-cold oxygenated (95% O2 ...
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bioRxiv - Microbiology 2021Quote: ... using QuantStudio 6 or 3 Flex Real-Time PCR System (Applied Biosystems). SARS-CoV-2 standards with known copy numbers were used to construct a standard curve and calculate copy numbers/mL or copy numbers/g.
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bioRxiv - Microbiology 2021Quote: ... Sections were cut at 3-5 μm on a cryotome (ThermoFisher Scientific) using C35 carbon steel blades (Feather ...
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bioRxiv - Cell Biology 2021Quote: ... JEG-3 cells were maintained in phenol red-free MEM (ThermoFisher #51200038) supplemented with the same additives and in addition 20 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 1 nl solution of 3 μM FM 4-64 (Molecular Probes, Invitrogen) dissolved in DMSO was injected in the otic cavity of 96 hpf zebrafish embryos mounted laterally in low gelling agarose (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... blotted for 3–4 s in a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Neuroscience 2020Quote: ... PSCs were loaded with the fluorescent Ca2+ indicator Rhod-3 (Life Technologies) by incubating muscles twice for 45 min in preoxygenated Ringer containing 5 μM Rhod-3 AM (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... NaCl (S271-3) and HCl (A144-212) were obtained from Fisher Scientific. For PER assays ...
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bioRxiv - Biochemistry 2021Quote: ... A predesigned TaqMan assay targeting exon 2–3 boundary (Hs00213726_m1; Life Technologies) was also used to ensure equal amount of the endogenous A4GALT transcript in transfected and non-transfected cells ...
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bioRxiv - Microbiology 2021Quote: ... to a DNA:lipofectamine ratio of 1:3 and 100μl Opti-MEM (Gibco). Cells were collected 24 hours post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-phospho-Pak1-2-3 (pSer141) (44-940G, 1:2000) from Invitrogen/Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: ... Jade1/2/3 mRNA was assessed by SYBR Green (Thermo Fisher Scientific) qPCR using Hprt1 as endogenous control ...
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bioRxiv - Cell Biology 2021Quote: ... phospho-PAK1/2/3 (Thr402) (ThermoFisher PA1-4636, 1:1000 for WB), phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: Calu-3 cells were cultured in RPMI 1640 (Life Technologies 22400-105) with 10% FBS (VWR 89510-186) ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 5 μl Turbo DNase buffer and 3 μl Turbo DNase (ThermoFisher Scientific) were added to each reaction and incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 5 µM CellEvent™ Caspase-3/7 Green Detection Reagent (ThermoFisher Scientific) were applied for monitoring effector caspase activation and 2.5 µM AlexaFluor647 hydrazide for detecting cell lysis.
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bioRxiv - Microbiology 2020Quote: ... Calu-3 2B4 cells were grown in MEM (GIBCO, Grand Island, NY) supplemented with 20% FBS ...
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bioRxiv - Microbiology 2020Quote: ... PobA activity was inhibited with methyl 4-hydroxy-3-iodobenzoate (Fisher Scientific) at a saturating concentration (0.48 mM) ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 3% fetal bovine serum (heat-inactivated; Thermo Fisher Scientific, Poland) and antibiotics ...
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bioRxiv - Microbiology 2021Quote: ... Stable transfectants were initially selected at 3 µg/ml Geneticin (ThermoFisher Scientific), and then maintained at 6 µg/ml Geneticin ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with Hank’s Balanced Salt Solution (HBSS, Gibco, 14025-092) and mechanically dissociated using borosilicate pipets with opening sizes of 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were grown for 3-4 days in Neurobasal medium (Thermo Fisher) supplemented with 2% FBS (HyClone) ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed on a QuantStudio 3 PCR machine (Applied Biosystems) using PowerUp Syber Green mastermix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells (3×105) were transiently transfected using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... (2002) on a QuantStudio™ 3 Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Synthetic Biology 2021Quote: ... samples were diluted 3:1 in 4× NuPAGE LDS sample buffer (Invitrogen) supplemented with 10% beta-mercaptoethanol (v/v) ...
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bioRxiv - Synthetic Biology 2021Quote: ... elegans were generated using 3 Fragment Multisite Gateway Cloning (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... nuclear extracts were separated using 3–8% Tris-Acetate gradient gels (Invitrogen). Total proteins on the gel were transferred to a PVDF membrane (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... at 4 °C for 3 h on a Labquake rotator (ThermoFisher Scientific). The beads were washed with 1 mL cold lysis/binding buffer four times at 300 g for 4 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... gels or NuPage Tris-Acetate 3-8% gels (Invitrogen, Carlsbad, CA, USA). Proteins were transferred using iBlot dry transfer system (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: PfJ2apt parasites were incubated with 3 mM divinyl sulfone (DVSF, Fisher Scientific) in 1x PBS for 30 minutes at 37°C ...