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Citations for Thermo Fisher :
8201 - 8250 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Human A549 or A549ACE2 cells were cultured in a Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco catalog no. 11875) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: ... used in this study were previously derived from in-house grown dermal fibroblasts from three healthy human donors reprogrammed with Cytotune Sendai viruses (ThermoFisher) (Ethics Committee ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Cell Biology 2024Quote: Human induced pluripotent stem cells (iPSCs) were obtained from Stanford Cardiovascular Institute and cultured in E8 medium (Gibco, Life Technologies) on 6-well plates coated with Matrigel (Corning) ...
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bioRxiv - Pathology 2024Quote: ... genomic DNA isolated from blood lymphocytes was subjected to exome capture using Agilent SureSelect human exome capture arrays (Life Technologies), followed by next generation sequencing on the Illumina® sequencing platform ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with goat anti-human IgM-FITC (1:100 dilution, Cat. no. A18842, Invitrogen), and IgG-FITC (1:200 dilution ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... Purified human primary pancreatic ductal cells (HPPD) were immediately cultured in a low-attachment plate in RMPI DMEM/F12 medium (Gibco), supplemented with 10% FBS and penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... The plate was washed three times using 200 µL of 1 x PBS containing 0.5 % Tween-20 and incubated with 100 µL of secondary antibody solution containing goat anti-human IgG conjugated with HRP (Cat. #A18805 from Invitrogen) at a dilution of 1:10000 in 5% BSA solution for 1hour ...
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bioRxiv - Microbiology 2024Quote: ... with a plasmid encoding for a human furin ectodomain (residues 1–794) into 40 mLs of FreeStyle 293-F cells (ThermoFisher). Cells were transfected at a density of 1 million cells/mL using 25 kDa linear polyethylenimine (PEI) ...
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bioRxiv - Microbiology 2024Quote: ... and FCR-CD36 strains were maintained in culture under standard conditions in O+ human erythrocytes in RPMI 1640 containing L-glutamine (Invitrogen) supplemented with 5% Albumax I ...
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bioRxiv - Biophysics 2024Quote: The immortalized human keratinocytes (HaCaT cells) were cultured in low calcium media made from no-calcium DMEM (21068028, ThermoFisher Scientific), 10% FBS and 1% penicillin-streptomycin at 37°C supplied with 5% CO2 to maintain the dividing state.
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bioRxiv - Immunology 2024Quote: ... Freshly purified CD8+ T cells (day 0) were activated by adding Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) at a cell/bead ratio of 1:0.5 for 48-64h and expanded in complete R10 medium [RPMI-1640 medium containing 20 mM HEPES and 2.05 mM L-glutamine (#7388 ...
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bioRxiv - Cell Biology 2024Quote: Previously generated microarray data from microdissected human glomeruli sourced from individuals with kidney disease and healthy donors were used (Affymetrix HGU133Plus2.0 ...
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bioRxiv - Immunology 2024Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Human fetal lung fibroblasts (MRC-5; ATCC, CCL-171) and human uterine cervix cancer cells (HeLa) were cultured in minimum essential medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Microarray assays were performed using Affymetrix GeneChip Rat Gene ST arrays (for McA cells) or GeneChip Human Gene 2.0 ST arrays (for Huh7 cells) (Applied Biosystem/Thermo Fisher). GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G running Affymetrix Gene Command Console ver 3.2 ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with Alexa Fluor 488-conjugated goat anti-human IgG to stain the RBD (Thermo Fisher Scientific; A11013) at 4℃ for 1 hour ...
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bioRxiv - Physiology 2024Quote: Cells from the human brain microvascular EC line hCMEC/D3 [37] were seeded on collagen I-coated 35 mm tissue culture dishes (Nunc) and grown in EGM2-MV (Lonza).
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bioRxiv - Microbiology 2024Quote: ... or the type II ME49 strain Δku80/Δhxgprt background (Waldman et al., 2020) were grown in human foreskin fibroblasts (HFFs, ATCC SCRC-1041) maintained in DMEM (GIBCO) supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... compound was incubated with cells expressing human S1PR1 and Gα15 in the culture medium consisting of Ham’s F-12 Nutrient Mixture (Thermo Fisher Scientific) supplemented with 10% fetal bovine serum for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The murine macrophage RAW264.7 cell line and Human colon adenocarcinoma Caco-2 cell line was cultured in DMEM (Gibco: 11995065) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Transcriptome analysis was done using the GeneChip Human Transcriptome Array 2.0 for in combination with the GeneChip WT PLUS Reagent kit (Applied Biosystems). 33,3ng total RNA was used as input for the analysis that was done according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... ELISAs for total IgM or IgG were also performed in parallel using human uncoated IgM or IgG ELISA kits (Invitrogen), following manufacturer’s instructions.
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bioRxiv - Biophysics 2024Quote: ... Secondary antibodies include donkey anti-human IgG Alexa Fluor 555 (1:600) and Goat anti-mouse IgG Alexa Fluor 405 (1:500, Invitrogen). Confocal microscopy was performed using a laser scanning confocal microscope (LSM 880 ...
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bioRxiv - Biochemistry 2024Quote: The human dynein motor domain locked in α-registry was expressed and purified from insect cells (ExpiSf9 cells; Life Technologies) as previously described (21) ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 mM L-glutamine at 37°C and in 5% CO2. Human RPE-1 cells (a gift from Dr. Hongtao Yu) were cultured in DMEM: F-12 medium (Invitrogen) supplemented with 10% FBS and 10mM L-glutamine at 37°C and in 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media (Gibco, Thermo Scientific, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin antibiotics at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Biochemistry 2024Quote: Human cervical carcinoma HeLa cells (CCL-2, ATCC) were cultured in DMEM/F-12 media (Gibco, Thermo Scientific, MA, USA) supplemented with 10% fetal calf serum and 1% penicillin/streptomycin antibiotics at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were transferred into a fresh falcon tube and gently resuspended in 14mL of human plasma-like media (HPLM) (Gibco) containing 10% dialysed FBS (dFBS ...
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bioRxiv - Biochemistry 2024Quote: The purified full-length human and mouse CNPase (hCNPase and mCNPase) were immobilised overnight at +4 °C on a 96-well immunosorbent plate (Nunc). All the following steps were done by gentle shaking on an orbital shaker ...
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bioRxiv - Cell Biology 2024Quote: Primary human skeletal muscle cells were seeded and differentiated on 4-well Nunc Lab-Tek II Chamber Slides (Thermo Scientific) coated with laminin (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes from PBMC buffy coats were isolated with anti-human CD14 magnetic beads (Miltenyi) and cultured in RPMI containing 10% heat-inactivated fetal bovine serum (FBS, Gibco), 50 U/ml penicillin ...
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bioRxiv - Microbiology 2024Quote: ... Colon tissue sections were prepared for immunofluorescence staining as previously described [27] using goat anti-human-AF647 and rabbit anti-GFP-AF488 conjugated antibodies (ThermoFisher, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... chronic infected patient and HD groups by Luminex using the Human Cytokine/Chemokine/Growth Factor 45-Plex ProcartaPlex Panel 1 (#EPX450-12171-901 ThermoFisher) according supplier’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... 1×106 cells were stained with the panel of antibodies in 100 µl of staining buffer containing 0.5 % human serum albumin (HSA, Albunorm, Octapharma) and 2 mM EDTA (ThermoFisher Scientific) in PBS (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: Immortalized human HaCaT keratinocytes [46] and murine K38 keratinocytes [59] were cultured in high glucose DMEM media (GIBCO 11965-092) supplied with penicillin and streptomycin (GIBCO 15140-122 ...
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bioRxiv - Cell Biology 2024Quote: ... Three technical replicates of 100 µg protein from each biological replicate diluted with diluent supplied by the manufacturer were added to the antibody-coated wells (Ferritin Human ELISA Kit, Invitrogen). All the steps were carried out following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The iPSCs were resuspended in human embryonic stem media with 10 ng/mL of bFGF (beta fibroblast growth factor, Invitrogen) (HES-10 ...
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bioRxiv - Cell Biology 2024Quote: ... the human embryonic kidney cell line HEK293T (purchased from ATCC) and the human hepatocellular carcinoma HepG2 (purchased from ATCC) were maintained in DMEM medium (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: The HT29-MTX human colorectal adenocarcinoma cell line 37 was grown in Dulbecco’s modified Eagle medium (DMEM, supplied by Thermo Fisher) supplemented with 10% heat-inactivated (HI ...
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bioRxiv - Molecular Biology 2020Quote: ... pcDNA-TruB1 was generated by inserting the ORF of human TruB1 with a Flag sequence into pcDNA.3.1(+) (Thermo Fisher Scientific) at the Nhe1 and EcoR1 sites ...
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bioRxiv - Cell Biology 2019Quote: ... and SqCC/Y1 cells and primary keratinocytes were routinely maintained in EpiLife medium (with 60 mM calcium concentration) (MEPI500CA) supplemented with Human Keratinocyte Growth Supplement (HKGS; ThermoFisher Scientific). For the experiments ...
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bioRxiv - Cell Biology 2020Quote: ... pseudokinase domain of mouse and human MLKL were expressed in Sf21 insect cells using the Bac-to-Bac system (ThermoFisher Scientific) and purified using established procedures28 ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Cell Biology 2019Quote: ... cells were subsequently pulsed at 370C for different time points with 25µg/ml Alexa flour-488 or- 568-conjugated human transferrin (Invitrogen, ThermoFisher Scientific) reconstituted in starvation medium and clarified by centrifugation at 15,000 × gmax at 40C for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... were prepared on the Ion Chef System using the Ion Ampliseq Chef DL8 materials and the Ion AmpliSeq Transcriptome Human Gene Expression Panel Chef-ready Kit (Thermo Fisher). Libraries were then purified via solid phase reversible immobilization (SPRI ...
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bioRxiv - Developmental Biology 2020Quote: ... Relative mRNA levels of a panel of inflammation-associated genes were initially screened using a Human Immune Taqman PCR array (4418718, ThermoFisher Scientific) and Taqman Fast Advanced Master Mix ...