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Citations for Thermo Fisher :
8051 - 8100 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated with corresponding secondary antibodies for 1 hr at room temperature: goat anti-human-Alexa-488 and goat anti-mouse-Alexa-488 (Invitrogen) for 293T internalization studies and goat anti-rabbit-Alexa-488 and goat anti-human-Alexa-555 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... 60 mm dishes of BHK-21 cells were transiently transfected with 2.5 μg human ACE2 or no DNA for control using standard lipofectamine 2000 (Life Technologies) protocols ...
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bioRxiv - Biochemistry 2022Quote: ... containing N-terminal ten histidine tag (His-tag) and human rhinovirus 3C (HRV 3C) protease site was amplified by PCR and cloned into the pFastBac vector (Invitrogen) using seamless cloning method ...
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bioRxiv - Neuroscience 2022Quote: ... Affymetrix Human Transcriptome Array 2.0 ST arrays were hybridized according Affymetrix recommendations using the Ambion WT protocol (Life technologies, France) and Affymetrix labelling and hybridization kits ...
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bioRxiv - Cell Biology 2022Quote: ... cells were then incubated with pre-warmed DMEM containing 20 µg/ml of TxR-Tfn (from human serum, Molecular Probes) and 0.1 % BSA for the specified times ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from rat glomeruli tissue and human podocyte samples using the mirVanaTM Isolation Kit (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Human CPα (CAPZA1, cloneID 3047937) and CPβ (CAPZB, clone ID 6715709) were purchased from Dharmacon and inserted into pcDNA3 (Invitrogen) with N-terminal MYC or FLAG tags ...
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bioRxiv - Cell Biology 2022Quote: Cryo-preserved primary human hepatocytes (PHH) from single donors (BD Gentest, Tewksbury, MA; Lonza, Walkersville, MD; and Thermo Fisher Scientific) were used for all in vitro experiments ...
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bioRxiv - Immunology 2022Quote: ... in culture media or stimulated for 48h with α-CD3/CD28 coated beads (Dynabeads Human T-Activator, Thermo Fisher scientific) in a 1:1 ratio (beads to cells) ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF-7 human breast cancer cells were obtained from ATCC and cultured in Roswell Park Memorial Institute medium (RPMI-1640; Gibco) supplemented with penicillin/streptomycin and 10% FCS ...
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bioRxiv - Cancer Biology 2022Quote: ... total cell RNA was extracted from FACS-purified LP or HP cell populations from B16-F10 murine or LM-MEL-28 human melanoma cells using PureLink RNA Mini kits (ThermoFisher), including on-column DNase treatment ...
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bioRxiv - Biophysics 2022Quote: ... polyclonal anti-human MPO (IgG, sheep, 1:50) (AA16-718, Antibody online), monoclonal anti-human Histone H1 (IgG2a, mouse, 1:100) (MA5-13750, Invitrogen), or monoclonal anti-human NPM1 (IgG1 ...
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bioRxiv - Cell Biology 2022Quote: ... >1.5 kb 5’and 3’ homology arms were amplified from genomic DNA isolated from one 6-cm dish of immortalized human retinal pigment epithelial cells (hTERT-RPE1 cells, ATCC) using a Purelink Genomic DNA Mini Kit (ThermoFisher). The π-element was PCR amplified from plasmid #1 ...
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bioRxiv - Cell Biology 2022Quote: Toxoplasma gondii RH∆hxgprt strain was maintained by growth in confluent primary human foreskin fibroblasts (HFFs) in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... the pFastBac plasmid containing full-length human dynein or tail-truncated dynein was transformed into DH10Bac competent cells (Gibco, #10361012) with heat shock at 42 °C for 45 seconds followed by incubation at 37 °C for 4 hours in S.O.C ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... were designed and used to create three different human-yeast hybrid genes by PCR (AccuPrime Pfx DNA polymerase from Invitrogen). The primers also add the attB1 and attB2 sites at the 5’ and 3’ ends of the PCR for cloning in pDONR221 entry clones using the BP cloning strategy ...
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bioRxiv - Immunology 2022Quote: ... freshly isolated or cryopreserved human PBMC or purified CD8+ T-cells (1×106) were cultured in complete culture medium (RPMI 1640 (Gibco) supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... Sorted Treg cells from HD and IPEX patients were stimulated with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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bioRxiv - Genomics 2022Quote: Purified human and bacterial DNA were quantified for concentration with the Qubit dsDNA HS assay (Life Technologies, Q33231, MA, USA), and pooled by adding 94% of human DNA ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone) primary mAb and FITC goat anti-mouse secondary antibody (Invitrogen). The cells were then fixed using 4% paraformaldehyde before staining the nuclei with Hoescht 33342 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: Telomerase-transduced Human Foreskin Fibroblasts (HFFs) and MRC5 lung fibroblasts were cultured in Dulbecco’s modified Eagle serum (DMEM; Invitrogen #11965118) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... then co-cultured at the indicated ratios and activated with Dynabeads Human T-Expander (1 bead:16 CD3+ T cells; Gibco) for 4 days ...
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bioRxiv - Synthetic Biology 2022Quote: ... cryopreserved PBMCs or T cells were thawed on day 0 and cultured with Human T-Activator αCD3/CD28 Dynabeads (Gibco) at 3:1 bead:cell ratio in AIM-V media (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... CVCL_1926) and HEK293T cells stably expressing human ACE2 (BEI NR-52511, RRID: CVCL_A7UK) were maintained in DMEM (Gibco, 11965-092) with 10% FBS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Molecular Biology 2022Quote: ... and 24 hours later cells were transfected with the Bluescript vector (empty vector control) and Human L1 plasmid (used in L1 retrotransposition assay) using Lipofectamine 2000 reagent (Invitrogen). Cells were harvested 36 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: SF268 (human glioblastoma) cells were cultured at 37 °C with 5% CO2 in RPMI medium supplemented with 10% FBS (Gibco). Vero (African green monkey kidney ...
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bioRxiv - Synthetic Biology 2023Quote: ... Syncytin1 was recoded based on the amino acid sequence from the The Human Protein Atlas and ordered as a gBlock (ThermoFisher). PCR reactions were performed using a high-fidelity polymerase (KOD-Xtreme ...
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bioRxiv - Microbiology 2022Quote: ... PBLs as well as human Jurkat T lymphocytes (human T-cell leukemia cell line) (ATCC-CRL-2899TM) were cultured in RPMI 1640 plus Glutamax (GIBCO) supplemented with 10% fetal bovin serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... 2×105 cells were transfected with 20 pmol of ON- TARGETplus Human HMG20A (10363) siRNA-SMART pool (Dharmacon) using Oligofectamine™ according to the manufacturer’s instructions (Invitrogen). Cells were cultured for 3 days before being harvested for follow-up experiments.
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bioRxiv - Neuroscience 2022Quote: ... human BDNF-biotin (Alomone labs; B-250-B) was conjugated to Quantum dots-605 (QD-605) (Thermo Fisher Scientific; Q10103MP). Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: Total RNA from isolated HeLa nucleoli was depleted of rRNA by the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Invitrogen). RNA was converted to double stranded cDNA using random hexamer with the NEBNext Ultra RNA First Strand and NEBNext Ultra RNA 2nd Strand Synthesis Kits (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... or Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (1:30 bead:T-cell ratio; Gibco) or (2 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Plant Biology 2024Quote: ... Recombinant protein with N-terminal 9x-His and GST tag was produced by amplifying the coding sequences of NRL5 and NRL5P335L and cloning into the expression vector pT7CFE1-NHIS-GST-CHA (Human In Vitro Protein Expression Vector, ThermoFisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... A mouse anti-human CD11b activated labeled with FITC (clone CBRM1/5, eBioscience) and elastin (EnzChek Elastase Assay Kit, ThermoFisher) were used in association with a rat anti-mouse LY6G antibody for the evaluation of neutrophils activation in cytometry ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Molecular Biology 2024Quote: ... the RAD54L2 cDNA was assembled from a partial cDNA clone encoding Met109 to Lys1467 was from the human ORFeome collection (Lamesch et al, 2007) and a synthetic DNA fragment encoding Met1 to Glu108 (Invitrogen). RAD54L2K311R was constructed by QuikChange site-directed mutagenesis with primers (5’-AGATCACTTGCAAAGTTCTCCCCAGACCCATGCTG-3’ and 5’-CAGCATGGGTCTGGGGAGAACTTTGCAAGTGATCT-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... that were derived from healthy human donor cells (isWT1.14, kindly provided by the UMG Stem Cell Unit) were cultured in StemFlex medium (Gibco A3349401) and differentiated to atrial cardiomyocytes according to an established protocol (Kleinsorge & Cyganek ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA encoding full-length human ADNP2 (amino acid residues 1–1131) was PCR amplified and cloned into a pFast-Bac-derived vector (Invitrogen) in frame with an N-terminal His6-tag ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked for 1h at room temperature with blocking buffer (3% skim milk powder dissolved in tris-buffered saline supplemented with 0.1% Tween-20 [TBS-T]) and then probed with CV3-25 mAb (1ug/mL) followed by HRP-conjugated anti-human IgG (1:3000, Invitrogen). HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences ...
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bioRxiv - Neuroscience 2024Quote: Organoid culture supernatants and lysates were analyzed for secreted proteins using single- and multiplexed Human ProcartaPlex™ Panel (Thermofisher Scientific), following previously documented procedures.37,38 The plates were read using the Luminex MAGPIX xMAP instrument and xPONENT® software ...
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bioRxiv - Microbiology 2024Quote: ... Huh7 human hepatoma and 293T cells (Riken, Japan) were grown and maintained in Dulbecco’s modified Eagle medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2024Quote: Human foreskin fibroblasts (HFFs) monolayers were grown in DMEM GlutaMAXTM (4.5 g/L D-glucose) (Life Technologies #10-566-024) supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...