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Citations for Thermo Fisher :
8151 - 8200 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells or MiniBAR-GFP stable cell lines were transfected with 25 nM of siRNAs targeting either luciferase or human Mini-BAR (5’-CUGCAAAUUUUACGGAUCA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... purified Treg cells (5 × 104) were cultured with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were incubated with 1:1000 diluted PE-conjugated F(ab’)2-anti-human-kappa-light-chain (Invitrogen, PA1-74408) in PBS/ 0.1% BSA (20 min ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Human TSC media (TSCm) used herein was previously described in 70 with slight modifications: 470 ml DMEM/F12 (Invitrogen 21331), 5 ml Commercial N2 supplement (Invitrogen 17502048) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Genomics 2023Quote: The human lung fibroblast cell line IMR-90 was purchased from ATCC and cultured in EMEM (ATCC) with 10% FBS (Gibco) and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: ... Unbound ACE2-Fc was removed by washing the cells with ice cold media and the bound ACE2 detected using goat anti-human secondary antibody conjugated to Alexa647 (ThermoFisher Scientific Cat ...
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bioRxiv - Genomics 2023Quote: ... These mRNAs were generated from cloned human cDNAs inserted into pCS2+ and in vitro transcribed using mMessage mMachine SP6 kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary human peripheral mononuclear blood cells (PBMCs) were cultured at 37 °C in 5% CO2 in RPMI 1640 (ThermoFisher, 61870143) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Transcriptome libraries were generated using automated library preparation with the Ion AmpliSeq Transcriptome Human Gene Expression Kit (Thermo Fisher Scientific). The transcriptome libraries were barcoded ...
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bioRxiv - Cell Biology 2023Quote: NK cells were transfected with scrambled or ON-TARGET plus human WWTR1 SMARTpool siRNA from Dharmacon Inc, (CCGCAGGGCUCAUGAGUAU, GGACAAACACCCAUGAACA, AGGAACAAACGUUGACUUA, CCAAAUCUCGUGAUGAAUC) using the Neon Transfection System (Invitrogen) and according to the manufacturer recommended parameters ...
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bioRxiv - Genomics 2023Quote: Pulmonary arterial adventitial cells isolated from idiopathic PAH and control lungs were obtained from PHBI and grown in Human Vascular Smooth Muscle Cell Basal Medium (M231500, ThermoFisher) supplemented with Smooth Muscle Growth Supplement (S00725 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for 2 hr at room temperature with the following antibodies at the manufacturer-recommended dilutions in 1× TFP Perm/Wash buffer: APC- eFluor780 anti-human CD3 (clone UCHT1, Invitrogen); PerCP-Cy5.5 anti-human CD4 (clone SK3 ...
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bioRxiv - Developmental Biology 2023Quote: ... floating on top of 500 µl of human lung ALI explant media (Advanced DMEM/F-12 (Thermo Fisher, Cat#12634010), 2 mM Glutamax (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... followed by C-terminal region of human FGF-21 (amino acids 166-209) into a vector pCEP4 (Thermo Fisher Scientific). The construct was then transiently transfected to Expi293F cells (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... The brain sections were then incubated with goat anti-mouse Alexa Fluor 488 and goat anti-human Alexa Fluor 647 (1:500, Invitrogen). Following secondary staining ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The donor template for homologous recombination was generated by PCR amplification of a human DNA sample carrying 51 CAG repeat into the pCR-Blunt II TOPO plasmid (Invitrogen). Subsequently ...
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bioRxiv - Cell Biology 2023Quote: ... in 6-well tissue culture plates at 37°C with 5% CO2 and 5% O2 They were grown in human embryonic stem cell (hESC-10) medium made up of a base of DMEM/F12 (50:50 Gibco) with 20% knock-out serum replacement (KOSR ...
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bioRxiv - Bioengineering 2023Quote: Undifferentiated H1 (WA01; XY) human embryonic stem cells (hESCs) were maintained on Geltrex coated plates (1:100 in DMEM/F12; Gibco) in StemFlex Medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: NIH 3T3 immortalised mouse fibroblast and U2OS human osteosarcoma cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice with PBS and 100 μL of Goat anti-human IgG (H+L) Alexa647 secondary antibody (2 μg/mL, Invitrogen) and Aqua viability dye was added for 20 minutes at RT ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Biochemistry 2023Quote: Human embryonic kidney cells (HEK293T, ATCC, Catalog# CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: iPSCs colonies were harvested with Accutase® and dissociated into single cells using the complete human embryonic stem cell (hESC) medium consisting of DMEM/F12 (11320033, ThermoFisher), 5x knockout serum replacement (10828010 ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... hTDP-43 mRNA was measured using the PrimeTime Gene Expression Master Mix (IDT) with the following TaqMan probes: human TDP-43 (Hs00606522_m1; ThermoFisher Scientific) and mouse Actb (Mm02619580_g1 ...
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bioRxiv - Immunology 2024Quote: All the siRNA-mediated knockdown experiments in primary human macrophages were performed by electroporation using a Neon Transfection System (MPK5000; Invitrogen). Each reaction used 1.2 – 1.5 x 106 hMDM ...
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bioRxiv - Systems Biology 2024Quote: All RAW files acquired from TMT-MS of all cases were searched against a human reference protein database using the Proteome Discoverer suite (version 2.4, ThermoFisher Scientific). MS2 spectra were searched against the UniProtKB human proteome database containing Swiss-Prot human reference protein sequences (20,338 target proteins downloaded in 2019) ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF-10A cells were transfected with the human ON-TARGET plus siRNA library targeting PHGDH (Dharmacon) or non-target control (Dharmacon) using the Neon Transfection System (Invitrogen). Then 96 hours post transcription ...
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bioRxiv - Neuroscience 2024Quote: The quantification of the total amount of soluble tau in S1 fractions was performed by using an ELISA kit (human Tau, Invitrogen), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... human ileocecal cell line from 25 to 35 passages were cultured in Dulbecco’s Modified Eagle’s medium (DMEM) (Thermo Fisher Scientific) supplemented with 4mM L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Microbiology 2023Quote: ... cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 (anti-human CD3 for NK cell experiments) according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Molecular Biology 2023Quote: ... One hundred microliters of cell suspension from each well was stained with anti-human CD45 according to the manufacturer’s instructions and washed twice with PBS (Gibco, USA) (300×g ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from human hippocampal/frontal cortex samples and cultured cells was extracted with mirVana’s isolation kit (Ambion, TX, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Bioengineering 2023Quote: Blood plasma extracted from each group (n=5) was analyzed using the Inflammation 20-Plex Human ProcartaPlex™ Panel (Invitrogen). Briefly ...
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bioRxiv - Biophysics 2023Quote: BHK-21 and CFBE41o- human CF bronchial epithelial cell lines were grown in DMEM/F-12 (5% fetal bovine serum; Invitrogen) and MEM (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: Protein from rat and human fetal lung explants was isolated by incubation with cell extraction buffer (ThermoFisher Scientific, Waltham, MA) supplemented with protease inhibitors (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 cells were transfected with 50nm ON-TARGETplus Human SMARCA4 siRNA (Dharmacon L-010431-00-0005) or the corresponding siControl (D0012061305) by lipofectamine RNAiMAX (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: DLD 1 human colorectal cancer and Colon26 mouse colon cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)(Gibco, USA) containing 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: ... MSC-EV preparation vesicular structure and canonical surface marker expression were determined using EV bead-based flow cytometry with human CD81 detection beads (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: We used the cytokine/chemokine/growth factor 45-Plex Human ProcartaPlex™ Panel 1 (Cat# EPX450-12171-901, ThermoFisher, USA) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Lipofectamine™ 3000 transfection reagent (InvitrogenTM, Cat# L3000001) and human PAR-1 si-RNA (CGGUCUGUUAUGUGUCUAUdTdT) were purchased from Invitrogen (USA) and Eurogentec (Belgium ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 106 human cells in 35 mm dish per sample were washed with 1 × PBS and lysed with 600 μl DNAzol (Invitrogen), followed by precipitation with 300 μl 200 proof ethanol ...
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bioRxiv - Cancer Biology 2023Quote: ... at different timepoints as the percentage of hCD20pos cells (anti-human CD20 FITC, clone 2H7) compared to murine CD45pos cells (anti-mouse CD45 PE, clone 30F11, ThermoFisher). When indicated ...
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bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: African green monkey kidney cells (Vero) and Human Embryonic Kidney 293T (HEK293T) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal Bovine Serum (FBS ...