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Citations for Thermo Fisher :
8451 - 8500 of 9091 citations for Recombinant Human SELE Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... samples were washed in PBS and a mixture of goat anti-Human Alexa488 conjugated secondary antibody (Thermo Fisher, diluted 1:2000) and DAPI (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Madin Darby canine kidney (MDCK) cells and human embryonic kidney 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: PBMC culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel (Thermofisher Scientific, Carlsbad, CA), following previously documented procedures.43,44 This is a preconfigured multiplex immunoassay kit that measures 65 cytokines ...
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bioRxiv - Immunology 2024Quote: ... T cells (1 million cells/ml) were activated with Dynabeads Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermo Fisher) in culture medium ...
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bioRxiv - Microbiology 2024Quote: Serum samples were analyzed using the Immune Monitoring 65-Plex Human ProcartaPlex Panel (Cat# EPX650-16500-901; ThermoFisher Scientific, Vienna, Austria), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Approximately 800,000 U2OS cells were reverse transfected with 20 nM siRNA against the 3’ UTR of endogenous TIRR or a negative control siRNA (Dharmacon ON-TARGETplus Human NUDT16L1 siRNA J-014872-23-0005 or ON-TARGETplus Non-targeting Control) using 3 uL of RNAiMAX lipofectamine (Thermo Fisher) in a 6-well plate ...
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bioRxiv - Neuroscience 2024Quote: ... Human peripheral blood mononuclear cells (ATCC #PCS-800-011) were thawed as recommended and resuspended in RPMI 1640 medium (Gibco #11875093) with 1X GlutaMAX (Gibco #35050061) ...
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bioRxiv - Genetics 2024Quote: Flag-tagged wild type and mutated human RBBP5 plasmid were transfected to HEK293 cells by lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, CA, USA). Cells were lysed by RIPA buffer and 20μg of whole cell lysate were used to assess protein expression in Western blot ...
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bioRxiv - Cancer Biology 2024Quote: ... #D-001810-10-05) or human GPX3 siRNA (Dharmacon, #L-006485-00-0005) were delivered to the cells using Lipofectamine RNAiMAX (Invitrogen, 13778150). After 24 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20 ...
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bioRxiv - Neuroscience 2023Quote: eGFP/Luc human iPSC-NSCs were seeded at a density of 1 x 104 cells per well in a Geltrex (Life Technologies) coated 96-well plate (flat bottom ...
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bioRxiv - Neuroscience 2023Quote: Low-passage-number Human embryonic kidney cells transformed with SV40 T-antigen (HEK293T) cells were cultured in DMEM (Thermo Fisher Scientific) with 10% fetal bovine serum at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Biophysics 2023Quote: ... University of Belgrade-Faculty of Biology which was previously produced by several reactions of cloning and subcloning of CTG repeats originating from human DMPK locus using pJet1.2/blunt cloning vector from CloneJET PCR Cloning Kit (Thermo Fisher Scientific). The subcloned region sequence was verified with Sanger sequencing before prior use ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Cell Biology 2024Quote: Cytokine secretion of PMA treated THP-1 cells was analyzed using Cytokine & Chemokine 34-Plex Human ProcartaPlex™ Panel 1A (Invitrogen) and measured with xMAP LuminexTM system (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Human TS cells were cultured in six-well plates precoated with 5 μg/mL of CorningTM collagen IV (CB40233, Thermo Fisher) and Basal Human TS Cell Medium [Dulbecco’s Modified Eagle Medium/F12 (DMEM/F12 ...
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bioRxiv - Genomics 2024Quote: ... and were activated for 72h using Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermofisher, 11161D) according to the manufacturer’s instructions at 37°C under 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... and were activated for 72h using Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation (Thermofisher, 11161D) according to the manufacturer’s instructions at 37°C under 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated overnight at 4°C with rat anti-human HLA-DR (clone: YD1/63.4) monoclonal antibody (Thermo-Fisher Scientific, USA), rabbit anti-human CD16 (clone ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from the CD8-depleted PBMC fraction using the MagniSort human CD4+ T cell enrichment kit (Thermo Fisher). T cell purity was routinely checked by flow cytometry (>80%) ...
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bioRxiv - Immunology 2024Quote: Human monocytic cell line THP1 (TIB-202, ATCC, Manassas, VA, USA) were seeded 106/ml on glass chamber slides (Thermo Scientific) precoated with poly-L-lysine (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... primary T cells were activated and stimulated with a 1:1 ratio of anti-human CD3/CD28 magnetic Dynabeads (Thermo Fisher) to cells in X-vivo 15 medium supplemented with 5% human AB serum (Heat inactivated) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ptf1aCreER;Insrf/f;nTnG acinar cells and the control cells were cultured with Human plasma-like media (Gibco, Cat. No. A4899102), instead of RPMI1640 ...
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bioRxiv - Biophysics 2024Quote: ... primary stained with goat anti-Syndecan-1 antibody and mouse anti-human choriogonadotropin (β-hCG) antibody (#14-6508-82, 1:500 dilution in the buffer solution, ThermoFisher), and secondary stained as previously described ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were then transfected with 25 nM of either ON-TARGETplus Human ABCB1 (5243) SMARTpool or non-targeting pool siRNAs (negative control, Dharmacon) using the Lipofectamine RNAiMAX reagents (Thermo Fisher). Following 24 hours of incubation ...
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bioRxiv - Bioengineering 2024Quote: ... Secondary antibodies or co-stains were as follows: Alexa Fluor 647-conjugated streptavidin (surface display staining other than TfR human/mouse cross-reactivity, ThermoFisher S21374); iFluor 647-conjugated anti-His-tag antibody (pilot CYpHER detection and TfR human/mouse cross-reactivity ...
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bioRxiv - Bioengineering 2024Quote: Human fibroblast (ATCC) and SKOV-3 cells (graciously donated from the Dr. Hoshino lab) were cultured on T75 flasks (Fisher Scientific) at 5% CO2 at 37° C using McCoys 5A media (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and PANC-1 (CRL-1469, ATCC) and the human colorectal cancer cell line HT29 (HTB-38, ATCC) were grown in RPMI-1640 (31870025, Thermofisher Scientific), DMEM (11965092 ...
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bioRxiv - Cancer Biology 2024Quote: ... and human gastrointestinal stromal tumour cells (GIST882) (National Infrastructure of Cell Line Resource, Beijing, China) were seeded in confocal dish (ThermoFisher Scientific) and grew in DMEM in 5% CO2 in a 37 °C incubator ...
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bioRxiv - Cell Biology 2024Quote: ... human EGF (10 µg/ml) and bovine insulin (10 µg/ml) in low-attachment plates (Nunclon sphera, Thermo Scientific™ #174932) and maintained at 37°C in a humidified 5% CO2 incubator for 3 days before harvesting ...
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bioRxiv - Microbiology 2023Quote: ... falciparum 3D7 blood-stage cultures were maintained at 4% hematocrit in human A+ erythrocytes in Rosewell Park Memorial Institute (RPMI) 1640/Hepes medium (Gibco, 11875119) supplemented with 0.5% Albumax II (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... and was then incubated (1:1000 dilution) for 1 hour with a mouse anti-human E-Cad ectodomain antibody (HECD-131700, 1:1000 dilution; Life Technologies). After two washes ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used in this study were all anti-human: STING (1:1000, CST, #13647 or 1:500, Thermo Fisher, MA526030), UBIQUITIN (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... a human brain organoid cultured during 4 months has been dissociated in TRIzol RNA isolation reagent (Thermo Fisher Scientific ref: 15596026). Extracted RNA has been processed with the NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The plates were then washed and incubated for 1 hour with horseradish peroxidase (HRP)-conjugated goat anti-human IgM (Invitrogen #A18835), goat anti-human IgA (Jackson #109-036-011) ...
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bioRxiv - Cell Biology 2023Quote: NIH 3T3 immortalised mouse fibroblast and U2OS human osteosarcoma cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 million SH-SY5Y cells stably overexpressing human A53T mutated α-synuclein (Danzer et al., 2007) were resuspended in electroporation buffer R (NEON electroporation system, ThermoFisher) and added to the antibody-fibril preparation (final volume 100 μl) ...
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bioRxiv - Cancer Biology 2022Quote: 10 ng of total RNA was reverse transcribed with MegaplexTM miRNA-specific stem-loop RT Primers Human Pool A v 2.1 (Thermo Fisher Scientific) and TaqMan® MicroRNA reverse transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... ribosomal RNA was depleted from the samples using the RiboMinus™ Human/Mouse Transcriptome Isolation Kit (Thermo Fisher Scientific, Waltham, MA). All samples were sequenced on an Illumina Hi-Seq sequencer to produce 150 bp paired-end reads ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-YB-1 and EGFP-YB-1 (S1 File) in human cells were synthesized and subcloned in the vector pcDNA3.1(+) at GeneArt (Thermo Fisher Scientific). FLAG-M2-2 gene was inserted between the NcoI and AflII restriction sites ...
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bioRxiv - Genetics 2023Quote: RT-qPCR experiments were performed as previously described (Coutton et al., 2019) with cDNAs extracted from various human tissues purchased from Life Technologies® ...
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bioRxiv - Bioengineering 2023Quote: The microdevices containing normal human lung fibroblasts with or without macrophages were fixed using a solution of 2% glutaraldehyde (ACROS organics) in PBS (containing Ca2+/Mg2+ ...
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bioRxiv - Immunology 2023Quote: ... or with rabbit-anti-human TG3 (PA596450, Thermo Fischer Scientific) followed by detection with Alexa 488 donkey anti-rabbit (Molecular Probes) antibody ...
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bioRxiv - Immunology 2023Quote: ... ROR2 (4-1) (heavy and light chains connected by human IgG3 upper+middle Hinge) were synthesized by GeneArt (ThermoFisher, Regensburg, Germany) and subcloned into lentiviral backbones containing IgG3- or CD8α-derived spacer domains ...
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bioRxiv - Microbiology 2023Quote: The human type II lung epithelial cell line A549 (ATCC CCL-185) was cultured in DMEM/F12 with GlutaMAX (Life Technologies) supplemented with 10% FBS (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Cell Biology 2023Quote: The C28/I2 human chondrocytes were cultured in T-75 flasks with Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen Corp., Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: Human cervical carcinoma cell line HeLa was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...